Mechanisms of HAHV-1 Interaction with Hemocytes in Haliotis diversicolor supertexta: An In Vitro Study
Biology,
Год журнала:
2025,
Номер
14(2), С. 121 - 121
Опубликована: Янв. 24, 2025
Haliotid
herpesvirus
1
(HAHV-1)
causes
significant
damage
to
the
abalone
aquaculture
industry.
Knowledge
of
HAHV-1
invasion
and
host
defense
mechanisms
is
limited
due
lack
stable
molluscan
cell
lines.
The
present
study
established
an
in
vitro
infection
model
using
primary
suspension
cultures
hemocytes
from
Haliotis
diversicolor
supertexta
discus
hannai.
cytopathic
effects
on
adherent-cultured
both
species
were
also
investigated.
DNA
loads
firstly
monitored
by
means
quantitative
PCR
during
development
viral
infection,
subsequently
mechanism
interaction
between
was
explored
a
transcriptome
analysis.
H.
exhibited
high
degree
susceptibility
HAHV-1,
with
reaching
peak
4.0
×
10⁷
copies/ng
DNA.
In
contrast,
no
replication
observed
hannai
hemocytes.
Transcriptome
analysis
revealed
that
evades
immune
response
early
stages
hijacks
host’s
energy
redox
metabolism
promote
its
at
late
stages.
Consequently,
this
provides
valuable
reference
point
for
investigation
virus−host
vitro.
Язык: Английский
Viral communities on the sandstone monuments of Angkor Wat, Bayon and Preah Vihear in Cambodia
International Biodeterioration & Biodegradation,
Год журнала:
2025,
Номер
199, С. 106024 - 106024
Опубликована: Фев. 17, 2025
Язык: Английский
Foot-and-mouth disease virus activates glycolysis and hijacks HK2 to inhibit innate immunity and promote viral replication
Veterinary Research,
Год журнала:
2025,
Номер
56(1)
Опубликована: Апрель 1, 2025
Abstract
Foot-and-mouth
disease
(FMD)
severely
restricts
the
healthy
development
of
global
animal
husbandry,
and
unclear
pathogenic
mechanism
FMD
virus
(FMDV)
leads
to
difficulty
in
preventing
purifying
FMD.
Glycolytic
remodelling
is
considered
one
hallmarks
viral
infection,
providing
energy
precursors
for
assembly
replication.
In
this
work,
interaction
between
FMDV
glycolysis
were
explored
from
perspective
immune
metabolism.
We
found
that
infection
increased
extracellular
acidification
rate,
lactic
acid
accumulation,
HK2
level.
addition,
during
enhances
glycolytic
activity
mediates
autophagic
degradation
IRF3/7
antagonize
innate
response,
thereby
promoting
Our
findings
provide
evidence
closely
correlated
with
host
metabolism,
increasing
understanding
facilitate
new
ideas
further
elucidating
FMDV.
Язык: Английский
Investigating how dengue virus-induced metabolic changes affect the host immune response and how to develop Immunomodulatory strategies
Virology Journal,
Год журнала:
2025,
Номер
22(1)
Опубликована: Апрель 25, 2025
Dengue
virus
(DENV)
infection
imposes
a
significant
global
health
burden,
driven
by
its
ability
to
manipulate
host
cellular
processes
facilitate
replication
and
evade
immune
defenses.
This
review
explores
the
complex
interplay
between
DENV,
immunometabolism,
signaling
pathways.
DENV
induces
metabolic
reprogramming,
including
glycolytic
upregulation,
lipid
droplet
utilization
through
lipophagy,
alterations
in
amino
acid
metabolism,
fulfill
energy
biosynthetic
needs.
The
also
disrupts
mitochondrial
dynamics,
leading
increased
reactive
oxygen
species
(ROS)
production,
which
modulates
responses
but
may
contribute
oxidative
stress
severe
pathology.
Concurrently,
hijacks
pathways,
PI3K/Akt,
NF-κB,
JAK/STAT,
suppress
apoptosis,
type
I
interferon
responses,
drive
pro-inflammatory
cytokine
production.
these
pathways
highlights
dual
role
of
processes:
enabling
viral
while
activating
antiviral
responses.
examines
potential
therapeutic
strategies
targeting
combat
infection,
glycolysis
inhibitors,
metabolism
modulators,
host-directed
therapies.
While
progress
has
been
made
understanding
DENV-induced
further
research
is
needed
elucidate
precise
molecular
mechanisms
translate
findings
into
clinical
applications.
study
underscores
importance
integrating
insights
identify
novel
targets
against
related
flaviviruses,
addressing
critical
need
for
effective
interventions.
Язык: Английский
Study on the detection rate, genetic polymorphism, viral load, persistent infection capacity, and pathogenicity of human papillomavirus type 33
Qiongyao Li,
Qimin Cheng,
Di Tian
и другие.
Virology Journal,
Год журнала:
2025,
Номер
22(1)
Опубликована: Апрель 26, 2025
There
is
a
lack
of
research
on
the
relations
among
genetic
polymorphisms,
viral
load,
adaptability,
persistent
infection
ability,
and
pathogenicity
human
papillomavirus
(HPV)
type
33.
Understanding
these
crucial
for
revealing
its
pathogenic
mechanisms
formulating
prevention
strategies.
Exfoliated
cervical
cells
were
harvested
from
female
participants
in
three
hospitals
located
southwestern
region
China
(Guizhou,
Sichuan,
Chongqing).
Real-time
fluorescence
PCR
technology
was
used
HPV
genotyping
genomic
quantification,
Sanger
sequencing
to
obtain
gene
sequence.
then,
changing
trends
HPV33
detection
rates
E6/E7
allele
frequencies
compared.
Positive
selection,
pathogenicity,
capacity
different
variants/mutations
analyzed.
Among
239,743
samples,
number
56,681,
rate
3.72%
(2,110/56,681)
all
detected
genotypes.
Between
2009
2023,
downward
trend
observed.
The
E6
+
E7
prototype
(E6
same
variant
consistent
with
reference
sequence)
dominant
variant,
significantly
increased
frequency.
This
showed
higher
relative
risk
causing
diseases
(cervical
intraepithelial
neoplasia
cancer).
load
disease
group
than
that
lesion-free
group,
clearance
group.
no
correlation
between
major
variants/mutations.
has
significant
impact
HPV33.
Viral
positively
correlated
capacity.
It
may
serve
as
biomarker
predicting
progression
during
screening.
Other
underlying
replacement
require
further
investigation.
Язык: Английский
Engineered cell membrane-based nano therapies fight infectious diseases
Journal of Controlled Release,
Год журнала:
2025,
Номер
unknown, С. 113884 - 113884
Опубликована: Май 1, 2025
Язык: Английский
Diverse virome and microbiome and their interactions for biodeterioration of Angkorian Bayon Temple in Cambodia
Опубликована: Май 1, 2025
Язык: Английский
STING exerts antiviral innate immune response by activating pentose phosphate pathway
Cell Communication and Signaling,
Год журнала:
2024,
Номер
22(1)
Опубликована: Дек. 18, 2024
The
innate
immune
system
serves
as
the
host's
first
line
of
defense
against
invading
pathogens.
Stimulator
interferon
genes
(STING)
is
a
key
component
this
system,
yet
its
relationship
with
glucose
metabolism,
particularly
in
antiviral
immunity,
remains
underexplored.
Metabolomics
analysis
was
used
for
detecting
metabolic
alterations
spleens
from
STING
knockout
(KO)
and
wild-type
(WT)
mice.
Co-immunoprecipitation
employed
determining
ubiquitination
TKT.
Mass
spectrometry
interaction
proteins
STING.
Enzyme
activity
kits
were
activities
TKT
G6PD.
In
study,
we
demonstrate
that
herpes
simplex
virus
(HSV)
infection
activates
pentose
phosphate
pathway
(PPP)
host
cells,
thereby
initiating
an
response.
Using
STING-manipulated
cells
systemic
mice,
show
positively
regulates
PPP,
which,
turn,
limits
HSV
infection.
Inhibition
PPP
significantly
reduced
production
factors
dampened
STING-induced
responses.
Mechanistically,
discovered
interacts
transketolase
(TKT),
enzyme
non-oxidative
branch
reduces
via
E3
ubiquitin
ligase
UBE3A,
stabilizing
Silencing
or
inhibiting
oxythiamine
diminished
factor
production.
Our
findings
reveal
plays
synergistic
role
generating
during
viral
suggest
activation
could
serve
adjunct
strategy
therapy.
Язык: Английский
Exploring the action mechanism and effective components of Yupingfeng powder on influenza based on computational system pharmacology and metabolomics
Journal of Ethnopharmacology,
Год журнала:
2024,
Номер
336, С. 118678 - 118678
Опубликована: Авг. 8, 2024
Yupingfeng
powder
(YPF)
is
a
classic
traditional
Chinese
medicine
prescription
with
long
history
of
clinical
application.
However,
there
consensus
on
the
efficacy
YPF
in
prevention
and
treatment
influenza,
underlying
pharmacological
mechanisms
functional
substances
have
not
been
thoroughly
investigated.
Язык: Английский