Mechanisms of HAHV-1 Interaction with Hemocytes in Haliotis diversicolor supertexta: An In Vitro Study

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 121 - 121

Published: Jan. 24, 2025

Haliotid herpesvirus 1 (HAHV-1) causes significant damage to the abalone aquaculture industry. Knowledge of HAHV-1 invasion and host defense mechanisms is limited due lack stable molluscan cell lines. The present study established an in vitro infection model using primary suspension cultures hemocytes from Haliotis diversicolor supertexta discus hannai. cytopathic effects on adherent-cultured both species were also investigated. DNA loads firstly monitored by means quantitative PCR during development viral infection, subsequently mechanism interaction between was explored a transcriptome analysis. H. exhibited high degree susceptibility HAHV-1, with reaching peak 4.0 × 10⁷ copies/ng DNA. In contrast, no replication observed hannai hemocytes. Transcriptome analysis revealed that evades immune response early stages hijacks host’s energy redox metabolism promote its at late stages. Consequently, this provides valuable reference point for investigation virus−host vitro.

Language: Английский

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Viral communities on the sandstone monuments of Angkor Wat, Bayon and Preah Vihear in Cambodia

International Biodeterioration & Biodegradation, Journal Year: 2025, Volume and Issue: 199, P. 106024 - 106024

Published: Feb. 17, 2025

Language: Английский

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Foot-and-mouth disease virus activates glycolysis and hijacks HK2 to inhibit innate immunity and promote viral replication

Veterinary Research, Journal Year: 2025, Volume and Issue: 56(1)

Published: April 1, 2025

Abstract Foot-and-mouth disease (FMD) severely restricts the healthy development of global animal husbandry, and unclear pathogenic mechanism FMD virus (FMDV) leads to difficulty in preventing purifying FMD. Glycolytic remodelling is considered one hallmarks viral infection, providing energy precursors for assembly replication. In this work, interaction between FMDV glycolysis were explored from perspective immune metabolism. We found that infection increased extracellular acidification rate, lactic acid accumulation, HK2 level. addition, during enhances glycolytic activity mediates autophagic degradation IRF3/7 antagonize innate response, thereby promoting Our findings provide evidence closely correlated with host metabolism, increasing understanding facilitate new ideas further elucidating FMDV.

Language: Английский

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Investigating how dengue virus-induced metabolic changes affect the host immune response and how to develop Immunomodulatory strategies

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 25, 2025

Dengue virus (DENV) infection imposes a significant global health burden, driven by its ability to manipulate host cellular processes facilitate replication and evade immune defenses. This review explores the complex interplay between DENV, immunometabolism, signaling pathways. DENV induces metabolic reprogramming, including glycolytic upregulation, lipid droplet utilization through lipophagy, alterations in amino acid metabolism, fulfill energy biosynthetic needs. The also disrupts mitochondrial dynamics, leading increased reactive oxygen species (ROS) production, which modulates responses but may contribute oxidative stress severe pathology. Concurrently, hijacks pathways, PI3K/Akt, NF-κB, JAK/STAT, suppress apoptosis, type I interferon responses, drive pro-inflammatory cytokine production. these pathways highlights dual role of processes: enabling viral while activating antiviral responses. examines potential therapeutic strategies targeting combat infection, glycolysis inhibitors, metabolism modulators, host-directed therapies. While progress has been made understanding DENV-induced further research is needed elucidate precise molecular mechanisms translate findings into clinical applications. study underscores importance integrating insights identify novel targets against related flaviviruses, addressing critical need for effective interventions.

Language: Английский

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Study on the detection rate, genetic polymorphism, viral load, persistent infection capacity, and pathogenicity of human papillomavirus type 33

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 26, 2025

There is a lack of research on the relations among genetic polymorphisms, viral load, adaptability, persistent infection ability, and pathogenicity human papillomavirus (HPV) type 33. Understanding these crucial for revealing its pathogenic mechanisms formulating prevention strategies. Exfoliated cervical cells were harvested from female participants in three hospitals located southwestern region China (Guizhou, Sichuan, Chongqing). Real-time fluorescence PCR technology was used HPV genotyping genomic quantification, Sanger sequencing to obtain gene sequence. then, changing trends HPV33 detection rates E6/E7 allele frequencies compared. Positive selection, pathogenicity, capacity different variants/mutations analyzed. Among 239,743 samples, number 56,681, rate 3.72% (2,110/56,681) all detected genotypes. Between 2009 2023, downward trend observed. The E6 + E7 prototype (E6 same variant consistent with reference sequence) dominant variant, significantly increased frequency. This showed higher relative risk causing diseases (cervical intraepithelial neoplasia cancer). load disease group than that lesion-free group, clearance group. no correlation between major variants/mutations. has significant impact HPV33. Viral positively correlated capacity. It may serve as biomarker predicting progression during screening. Other underlying replacement require further investigation.

Language: Английский

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Engineered cell membrane-based nano therapies fight infectious diseases

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113884 - 113884

Published: May 1, 2025

Language: Английский

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Diverse virome and microbiome and their interactions for biodeterioration of Angkorian Bayon Temple in Cambodia

Published: May 1, 2025

Language: Английский

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STING exerts antiviral innate immune response by activating pentose phosphate pathway

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 18, 2024

The innate immune system serves as the host's first line of defense against invading pathogens. Stimulator interferon genes (STING) is a key component this system, yet its relationship with glucose metabolism, particularly in antiviral immunity, remains underexplored. Metabolomics analysis was used for detecting metabolic alterations spleens from STING knockout (KO) and wild-type (WT) mice. Co-immunoprecipitation employed determining ubiquitination TKT. Mass spectrometry interaction proteins STING. Enzyme activity kits were activities TKT G6PD. In study, we demonstrate that herpes simplex virus (HSV) infection activates pentose phosphate pathway (PPP) host cells, thereby initiating an response. Using STING-manipulated cells systemic mice, show positively regulates PPP, which, turn, limits HSV infection. Inhibition PPP significantly reduced production factors dampened STING-induced responses. Mechanistically, discovered interacts transketolase (TKT), enzyme non-oxidative branch reduces via E3 ubiquitin ligase UBE3A, stabilizing Silencing or inhibiting oxythiamine diminished factor production. Our findings reveal plays synergistic role generating during viral suggest activation could serve adjunct strategy therapy.

Language: Английский

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Exploring the action mechanism and effective components of Yupingfeng powder on influenza based on computational system pharmacology and metabolomics

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 336, P. 118678 - 118678

Published: Aug. 8, 2024

Yupingfeng powder (YPF) is a classic traditional Chinese medicine prescription with long history of clinical application. However, there consensus on the efficacy YPF in prevention and treatment influenza, underlying pharmacological mechanisms functional substances have not been thoroughly investigated.

Language: Английский

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