Biomaterials Science,
Год журнала:
2023,
Номер
12(1), С. 8 - 56
Опубликована: Окт. 25, 2023
Hydrogels
have
established
their
significance
as
prominent
biomaterials
within
the
realm
of
biomedical
research.
However,
injectable
hydrogels
garnered
greater
attention
compared
with
conventional
counterparts
due
to
excellent
minimally
invasive
nature
and
adaptive
behavior
post-injection.
With
rapid
advancement
emerging
chemistry
deepened
understanding
biological
processes,
contemporary
been
endowed
an
"intelligent"
capacity
respond
various
endogenous/exogenous
stimuli
(such
temperature,
pH,
light
magnetic
field).
This
innovation
has
spearheaded
revolutionary
transformations
across
fields
such
tissue
engineering
repair,
controlled
drug
delivery,
disease-responsive
therapies,
beyond.
In
this
review,
we
comprehensively
expound
upon
raw
materials
(including
natural
synthetic
materials)
principles
these
advanced
hydrogels,
concurrently
providing
a
detailed
discussion
prevalent
strategies
for
conferring
stimulus
responsiveness.
Finally,
elucidate
latest
applications
"smart"
stimuli-responsive
in
domain,
offering
insights
into
prospects.
Pharmaceutics,
Год журнала:
2022,
Номер
14(6), С. 1177 - 1177
Опубликована: Май 31, 2022
Tissue
engineering
has
become
a
medical
alternative
in
this
society
with
an
ever-increasing
lifespan.
Advances
the
areas
of
technology
and
biomaterials
have
facilitated
use
engineered
constructs
for
issues.
This
review
discusses
on-going
concerns
latest
developments
widely
employed
biomaterial
field
tissue
engineering:
gelatin.
Emerging
techniques
including
3D
bioprinting
gelatin
functionalization
demonstrated
better
mimicking
native
by
reinforcing
gelatin-based
systems,
among
others.
breakthrough
facilitates,
on
one
hand,
manufacturing
process
when
it
comes
to
practicality
cost-effectiveness,
which
plays
key
role
transition
towards
clinical
application.
On
other
can
be
concluded
that
could
considered
as
promising
future
trends,
focus
might
detection
diagnosis
diseases
rather
than
treatment.
Abstract
Paracrine
is
an
important
mechanism
in
mesenchymal
stem
cells
(MSCs)
that
promotes
tissue
regeneration.
However,
anoikis
attributed
to
unsuitable
adhesion
microenvironment
hindered
this
paracrine
effect.
In
study,
a
living
and
injectable
porous
hydrogel
microsphere
with
long‐term
activity
constructed
via
the
freeze‐drying
microfluidic
technology
incorporation
of
platelet‐derived
growth
factor‐BB
(PDGF‐BB)
exogenous
MSCs.
Benefiting
from
structure
superior
mechanical
property
methacrylate
gelatin
(GelMA)
microspheres
(GMs),
are
able
adhere
proliferate
on
GMs,
thereby
facilitating
cell‐to‐extracellular
matrix
(ECM)
cell‐to‐cell
interactions
enhancing
Furthermore,
sustained
release
PDGF‐BB
can
recruit
endogenous
MSCs
prolong
GMs.
vitro
vivo
experiments
validated
GMs
exhibit
secretion
properties
anti‐inflammatory
efficacy
attenuate
osteoarthritis
(OA)
progression
by
favoring
adherent
utilizing
synergistic
effect
Overall,
enhance
fabricated
anticipated
hold
potential
future
clinical
translation
OA
other
diseases.
Bioactive Materials,
Год журнала:
2022,
Номер
14, С. 377 - 388
Опубликована: Фев. 1, 2022
Exosomes
derived
from
mesenchymal
stem
cells
(MSCs)
have
demonstrated
regenerative
potential
for
cell-free
bone
tissue
engineering,
nevertheless,
certain
challenges,
including
the
confined
therapeutic
potency
of
exosomes
and
ineffective
delivery
method,
are
still
persisted.
Here,
we
confirmed
that
hypoxic
precondition
could
induce
enhanced
secretion
human
exfoliated
deciduous
teeth
(SHEDs)
via
comprehensive
proteomics
analysis,
corresponding
(H-Exo)
exhibited
superior
in
promoting
cellular
angiogenesis
osteogenesis
significant
up-regulation
focal
adhesion,
VEGF
signaling
pathway,
thyroid
hormone
synthesis.
Then,
developed
a
platform
technology
enabling
effective
with
sustained
release
kinetics
to
irregular-shaped
defects
injection.
This
is
based
on
simple
adsorbing
technique,
where
adsorbed
onto
surface
injectable
porous
poly(lactide-co-glycolide)
(PLGA)
microspheres
bioinspired
polydopamine
(PDA)
coating
(PMS-PDA
microspheres).
The
PMS-PDA
effectively
adsorb
exosomes,
show
H-Exo
21
days
high
bioactivity,
vascularized
regeneration
5-mm
rat
calvarial
defect.
These
findings
indicate
microsphere-based
exosome
efficient
inducing
regeneration,
hence
facilitating
clinical
translation
exosome-based
therapy.
Advanced Materials,
Год журнала:
2023,
Номер
35(18)
Опубликована: Фев. 13, 2023
Aging
impairs
tendon
stem/progenitor
cell
function
and
homeostasis,
however,
effective
treatments
for
aging-induced
diseases
are
lacking.
Exosomes
naturally
derived
nanoparticles
that
contain
bioactive
molecules,
therefore,
have
attracted
great
interest
in
tissue
engineering
regenerative
medicine.
In
this
study,
it
is
shown
young
exosomes
secreted
by
stem
cells
from
human
exfoliated
deciduous
teeth
(SHED-Exos)
possess
abundant
anti-aging
signals.
These
bio-nanoparticles
can
alleviate
the
aging
phenotypes
of
aged
(AT-SCs)
maintain
their
tenogenic
capacity.
Mechanistically,
SHED-Exos
modulate
histone
methylation
inhibit
nuclear
factor-κB
to
reverse
AT-SC
aging.
a
mouse
model,
systemic
administration
SHED-Exo
retards
degeneration.
Interestingly,
local
delivery
SHED-Exos-loaded
microspheres
confers
phenotypes,
including
reduced
senescent
decreased
ectopic
bone
formation,
thereby
functionally
structurally
rescuing
endogenous
regeneration
repair
capacity
rats.
Overall,
SHED-Exos,
as
natural
nanoparticles,
promising
translational
therapeutic
potential
aging-related
diseases.
Rapid
and
effective
repair
of
injured
or
diseased
bone
defects
remains
a
major
challenge
due
to
shortages
implants.
Smart
hydrogels
that
respond
internal
external
stimuli
achieve
therapeutic
actions
in
spatially
temporally
controlled
manner
have
recently
attracted
much
attention
for
therapy
regeneration.
These
can
be
modified
by
introducing
responsive
moieties
embedding
nanoparticles
increase
their
capacity
repair.
Under
specific
stimuli,
smart
variable,
programmable,
controllable
changes
on
demand
modulate
the
microenvironment
promoting
healing.
In
this
review,
we
highlight
advantages
summarize
materials,
gelation
methods,
properties.
Then,
overview
recent
advances
developing
biochemical
signals,
electromagnetic
energy,
physical
including
single,
dual,
multiple
types
enable
physiological
pathological
modulating
microenvironment.
discuss
current
challenges
future
perspectives
regarding
clinical
translation
hydrogels.
Biomacromolecules,
Год журнала:
2023,
Номер
25(4), С. 2075 - 2113
Опубликована: Июль 5, 2023
The
field
of
bone
tissue
engineering
has
seen
significant
advancements
in
recent
years.
Each
year,
over
two
million
transplants
are
performed
globally,
and
conventional
treatments,
such
as
grafts
metallic
implants,
have
their
limitations.
Tissue
offers
a
new
level
treatment,
allowing
for
the
creation
living
within
biomaterial
framework.
Recent
advances
biomaterials
provided
innovative
approaches
to
rebuilding
function
after
damage.
Among
them,
gelatin
methacryloyl
(GelMA)
hydrogel
is
emerging
promising
supporting
cell
proliferation
regeneration,
GelMA
exhibited
exceptional
physicochemical
biological
properties,
making
it
viable
option
clinical
translation.
Various
methods
classes
additives
been
used
application
with
incorporation
nanofillers
or
other
polymers
enhancing
its
resilience
functional
performance.
Despite
results,
fabrication
complex
structures
that
mimic
architecture
provision
balanced
physical
properties
both
vasculature
growth
proper
stiffness
load
bearing
remain
challenges.
In
terms
utilizing
osteogenic
additives,
priority
should
be
on
versatile
components
promote
angiogenesis
osteogenesis
while
reinforcing
structure
applications.
This
review
focuses
efforts
advantages
GelMA-based
composite
engineering,
covering
literature
from
last
five
Abstract
Bone
defects
are
encountered
substantially
in
clinical
practice,
and
bionic
scaffolds
represent
a
promising
solution
for
repairing
bone
defects.
However,
it
is
difficult
to
fabricate
with
structures
reconstruct
the
microenvironment
fulfill
satisfying
repair
effects.
In
this
review
article,
we
first
discuss
various
strategies
design
construction
of
promote
defect
repair,
especially
including
structural
scaffold
integration
bioactive
substances
together
application
external
stimuli.
We
then
roles
artificial
intelligence
medical
imaging
aiding
treatment.
Finally,
point
out
challenges
future
outlooks
developing
multifunctional
scaffolds,
aiming
provide
insights
improving
regeneration
efficacy
accelerating
translation.
Biomacromolecules,
Год журнала:
2023,
Номер
24(6), С. 2928 - 2941
Опубликована: Май 22, 2023
Gelatin
methacryloyl
(GelMA)
hydrogels
have
been
extensively
used
for
drug
delivery
and
tissue
engineering
applications
due
to
their
good
biocompatibility,
biodegradability,
controllable
photocurable
efficiency.
Phosphate
buffer
solution
(PBS)
is
the
most
widely
reaction
system
GelMA
synthesis.
However,
carbonate–bicarbonate
(CBS)
has
tried
recently
synthesizing
its
high
there
a
lack
of
systematic
investigation
into
possible
differences
in
structure
properties
synthesized
PBS
CBS,
respectively.
Therefore,
current
study,
molecules
with
two
degrees
methacryloylation
(∼20
∼80%)
were
under
CBS
systems,
respectively,
comparable
conditions.
The
results
showed
that
because
functionalization
methacrylate
groups
gelatin
chains,
which
could
interfere
intrachain
interchain
interactions,
such
as
hydrogen
bonding,
had
distinct
physical
structures
exhibited
different
comparison
those
produced
CBS.
higher
gel–sol
transition
temperatures
better
efficiencies,
mechanical
strength,
biological
properties.
In
contrast,
advantages
swelling
performance
microstructures,
pore
sizes
porosities.
addition,
possessing
degree
(the
"GelMA-PH"
polymer)
great
potential
three-dimensional
(3D)
bioprinting.
This
focused
study
gained
helpful
new
insights
can
provide
guidance
on
application
3D
printing
engineering.
