Fabricating biomimetic materials with ice‐templating for biomedical applications DOI Creative Commons
Xiang Lin,

Lu Fan,

Li Wang

и другие.

Smart Medicine, Год журнала: 2023, Номер 2(3)

Опубликована: Июль 5, 2023

The proper organization of cells and tissues is essential for their functionalization in living organisms. To create materials that mimic natural structures, researchers have developed techniques such as patterning, templating, printing. Although these own several advantages, processes still involve complexity, are time-consuming, high cost. better simulate with micro/nanostructures evolved millions years, the use ice templates has emerged a promising method producing biomimetic more efficiently. This article explores historical approaches taken to produce traditional structural biomaterials delves into principles underlying ice-template various applications creation materials. It also discusses most recent biomedical uses created via templates, including porous microcarriers, tissue engineering scaffolds, smart Finally, challenges potential current technology analyzed.

Язык: Английский

Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions DOI Creative Commons

Peilin Lu,

Dongxue Ruan,

Meiqi Huang

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Июль 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Язык: Английский

Процитировано

78
A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing DOI Creative Commons
Jinjian Huang, Rong Yang, Jiao Jiao

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Ноя. 29, 2023

High glucose-induced vascular endothelial injury is a major pathological factor involved in non-healing diabetic wounds. To interrupt this process, we design an all-peptide printable hydrogel platform based on highly efficient and precise one-step click chemistry of thiolated γ-polyglutamic acid, glycidyl methacrylate-conjugated arginine-glycine-aspartate sequences. Vascular growth 165-overexpressed human umbilical vein cells are printed using platform, hence fabricating living material with high cell viability spatial distribution control. This cell-laden accelerates the wound healing rats unabated 165 release, which promotes angiogenesis alleviates damages mitochondria, thereby reducing tissue hypoxia, downregulating inflammation, facilitating extracellular matrix remodeling. Together, study offers promising strategy for tissue-friendly, high-efficient, accurate 3D delivery self-renewable therapy.

Язык: Английский

Процитировано

66
Boosting cartilage repair with silk fibroin-DNA hydrogel-based cartilage organoid precursor DOI Creative Commons

Congyi Shen,

Jian Wang, Guangfeng Li

и другие.

Bioactive Materials, Год журнала: 2024, Номер 35, С. 429 - 444

Опубликована: Фев. 16, 2024

Osteoarthritis (OA), a common degenerative disease, is characterized by high disability and imposes substantial economic impacts on individuals society. Current clinical treatments remain inadequate for effectively managing OA. Organoids, miniature 3D tissue structures from directed differentiation of stem or progenitor cells, mimic native organ functions. They are useful drug testing serve as active grafts repair. However, organoid construction requires extracellular matrix-like scaffolds cellular growth. Hydrogel microspheres, with tunable physical chemical properties, show promise in cartilage engineering replicating the natural microenvironment. Building prior work SF-DNA dual-network hydrogels regeneration, we developed novel RGD-SF-DNA hydrogel microsphere (RSD-MS) via microfluidic system integrating photopolymerization self-assembly techniques then modified Pep-RGDfKA. The RSD-MSs exhibited uniform size, porous surface, optimal swelling degradation properties.

Язык: Английский

Процитировано

53
Mitochondrial-Oriented Injectable Hydrogel Microspheres Maintain Homeostasis of Chondrocyte Metabolism to Promote Subcellular Therapy in Osteoarthritis DOI Creative Commons
Li Chen,

Jianye Yang,

Zhengwei Cai

и другие.

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Subcellular mitochondria serve as sensors for energy metabolism and redox balance, the dynamic regulation of functional dysfunctional plays a crucial role in determining cells' fate. Selective removal at subcellular level can provide chondrocytes with to prevent degeneration, thereby treating osteoarthritis. Herein, achieve an ideal therapy, cartilage affinity peptide (WYRGRL)-decorated liposomes loaded mitophagy activator (urolithin A) were integrated into hyaluronic acid methacrylate hydrogel microspheres through microfluidic technology, named HM@WY-Lip/UA, that could efficiently target selectively remove mitochondria. As result, this system demonstrated advantage function restoration, reactive oxygen species scavenging, cell survival rescue, chondrocyte homeostasis maintenance increasing mitophagy. In rat post-traumatic osteoarthritis model, intra-articular injection HM@WY-Lip/UA ameliorated matrix degradation, osteophyte formation, subchondral bone sclerosis 8 weeks. Overall, study indicated provided protective effect on degeneration efficacious clinically relevant manner, mitochondrial-oriented strategy has great potential therapy

Язык: Английский

Процитировано

22
Injectable nanocomposite hydrogels with enhanced lubrication and antioxidant properties for the treatment of osteoarthritis DOI Creative Commons
Qizhu Chen, Yuxin Jin, Tao Chen

и другие.

Materials Today Bio, Год журнала: 2024, Номер 25, С. 100993 - 100993

Опубликована: Фев. 22, 2024

Osteoarthritis (OA) is a chronic inflammatory joint disease characterized by progressive cartilage degeneration, synovitis, and osteoid formation. In order to effectively treat OA, it important block the harmful feedback caused reactive oxygen species (ROS) produced during wear. To address this challenge, we have developed injectable nanocomposite hydrogels composed of polygallate-Mn (PGA-Mn) nanoparticles, oxidized sodium alginate, gelatin. The inclusion PGA-Mn not only enhances mechanical strength biohydrogel through Schiff base reaction with gelatin but also ensures efficient ROS scavenging ability. Importantly, hydrogel exhibits excellent biocompatibility, allowing remove from chondrocytes reduce expression factors within joint. Additionally, hygroscopic properties contribute reduced intra-articular friction promote production cartilage-related proteins, supporting synthesis. vivo experiments involving injection into rat knee joints an OA model demonstrated successful reduction osteophyte formation protection wear, highlighting therapeutic potential approach for treating OA.

Язык: Английский

Процитировано

18
Capturing cerium ions via hydrogel microspheres promotes vascularization for bone regeneration DOI Creative Commons
Junlin Liu,

Zhangzhe Zhou,

Mingzhuang Hou

и другие.

Materials Today Bio, Год журнала: 2024, Номер 25, С. 100956 - 100956

Опубликована: Янв. 25, 2024

The rational design of multifunctional biomaterials with hierarchical porous structure and on-demand biological activity is great consequence for bone tissue engineering (BTE) in the contemporary world. advanced combination trace element cerium ions (Ce3+) repair materials makes composite material capable promoting angiogenesis enhancing osteoblast activity. Herein, a living phosphorylated injectable hydrogel microsphere (P-GelMA-Ce@BMSCs) constructed by microfluidic technology coordination reaction metal ion ligands while loaded exogenous BMSCs. Exogenous stem cells can adhere to proliferate on microspheres, thus cell-extracellular matrix (ECM) cell-cell interactions. active ingredient Ce3+ promotes proliferation, osteogenic differentiation rat BMSCs, endotheliocytes mineral deposition, gene expression, VEGF secretion. enhancement osteogenesis improvement P-GelMA-Ce scaffold mainly associated activation Wnt/β-catenin pathway. This study could provide novel meaningful insights treating defects biofunctional basis ions.

Язык: Английский

Процитировано

16
Engineered protein-based materials for tissue repair: A review DOI
Jiayin Feng, Fangyan Wang,

Yunyuan Shao

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 303, С. 140674 - 140674

Опубликована: Фев. 3, 2025

Язык: Английский

Процитировано

4
Hydrogen Ion Capturing Hydrogel Microspheres for Reversing Inflammaging DOI
Dandan Zheng, Wei Chen, Tongtong Chen

и другие.

Advanced Materials, Год журнала: 2023, Номер 36(5)

Опубликована: Сен. 12, 2023

Inflammaging is deeply involved in aging-related diseases and can be destructive during aging. The maintenance of pH balance the extracellular microenvironment alleviate inflammaging repair tissue damage. In this study, hydrogen ion capturing hydrogel microsphere (GMNP) composed mineralized transforming growth factor-β (TGF-β) catalase (CAT) nanoparticles developed via biomimetic mineralization microfluidic technology for blocking NLRP3 cascade axis inflammaging. This GMNP neutralize acidic by excess ions through calcium carbonate layer. Then, subsequent release encapsulated TGF-β CAT eliminate both endogenous exogenous stimulus NLRP3, thus suppressing excessive activation vitro, suppress TXNIP/NLRP3/IL-1β enhance matrix (ECM) synthesis nucleus pulposus cells. vivo, becomes a sustainable stable niche with microspheres as core to inhibit promote regeneration degenerated intervertebral discs. Therefore, ion-capturing effectively reverses interfering tissues.

Язык: Английский

Процитировано

34
Injectable and Degradable POSS–Polyphosphate–Polysaccharide Hybrid Hydrogel Scaffold for Cartilage Regeneration DOI

Leisha Cui,

Zun Yang,

Jing Hong

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2023, Номер 15(17), С. 20625 - 20637

Опубликована: Апрель 20, 2023

The limited self-repair capacity of articular cartilage has motivated the development stem cell therapy based on artificial scaffolds that mimic extracellular matrix (ECM) tissue. In view specificity cartilage, desirable tissue adhesiveness and stable mechanical properties under cyclic loads are critical for scaffolds. Herein, we developed an injectable degradable organic-inorganic hybrid hydrogel as a scaffold polyhedral oligomeric silsesquioxane (POSS)-cored polyphosphate polysaccharide. Specifically, acrylated 8-arm star-shaped POSS-poly(ethyl ethylene phosphate) (POSS-8PEEP-AC) was synthesized cross-linked with thiolated hyaluronic acid (HA-SH) to form POSS-PEEP/HA hydrogel. Incorporation POSS in increased properties. showed enzymatic biodegradability favorable biocompatibility, supporting growth differentiation human mesenchymal cells (hMSCs). chondrogenic encapsulated hMSCs promoted by loading transforming factor-β3 (TGF-β3) addition, capable adhering rat resisting compression. Furthermore, vivo results revealed transplanted significantly improved regeneration rats, while conjugation TGF-β3 achieved better therapeutic effect. present work demonstrated potential injectable, biodegradable, mechanically enhanced biomaterial regeneration.

Язык: Английский

Процитировано

23
Hydrogels for ameliorating osteoarthritis: Mechanical modulation, anti‐inflammation, and regeneration DOI Creative Commons

Xuwei Jiang,

Sun Yu-xiang,

Yuanning Lyu

и другие.

BMEMat, Год журнала: 2024, Номер 2(2)

Опубликована: Март 1, 2024

Abstract Osteoarthritis (OA) is a chronic and degenerative disease with limited clinical options for effective suppression. Recently, significant endeavors have been explored to reveal its pathogenesis develop treatments against OA. Hydrogels, designed striking resemblance the extracellular matrix, offer biomimetic interaction biological tissues, presenting promising avenue OA amelioration. As result, biocompatible hydrogels erected incorporating on‐demand bioactivities optimize intra‐articular microenvironment, thereby alleviating symptoms fostering eventual regeneration of articular joints. This review highlights collaborative objectives underlying establishment this tissue encompassing mechanical modulation, anti‐inflammation, regeneration. Specifically, we consolidate recent advances in hydrogel‐based biomaterials, serving as engineering scaffolds replicate lubrication properties natural joints or bioactive agent‐loaded vehicles combat localized inflammation. Additionally, function cell facilitate maintenance cellular homeostasis contribute advancement cartilage Finally, outlines prospective directions hydrogel‐mediated therapies.

Язык: Английский

Процитировано

15