Multienzyme‐Like Nanozymes: Regulation, Rational Design, and Application

Advanced Materials, Год журнала: 2023, Номер 36(10)

Опубликована: Фев. 25, 2023

Nanomaterials with more than one enzyme-like activity are termed multienzymic nanozymes, and they have received increasing attention in recent years hold huge potential to be applied diverse fields, especially for biosensing therapeutics. Compared single nanozymes offer various unique advantages, including synergistic effects, cascaded reactions, environmentally responsive selectivity. Nevertheless, along these merits, the catalytic mechanism rational design of complicated elusive as compared single-enzymic nanozymes. In this review, classification scheme based on numbers/types activities, internal external factors regulating multienzymatic chemical, biomimetic, computer-aided strategies, progress applications attributed advantages multicatalytic activities systematically discussed. Finally, current challenges future perspectives regarding development application suggested. This review aims deepen understanding inspire research a greater extent.

Язык: Английский

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A novel fluorescence biosensor based on CRISPR/Cas12a integrated MXenes for detecting Aflatoxin B1

Talanta, Год журнала: 2022, Номер 252, С. 123773 - 123773

Опубликована: Авг. 6, 2022

Aflatoxin B1 (AFB1) contamination in food threatens global safety, and rapid quantitative detection of AFB1 remains a challenge. Herein, novel fluorescence biosensor was developed for based on CRISPR/Cas12a MXenes. Specifically, the well-designed activator locked by dual-AFB1 aptamers, Cas12a directly linked to crRNA form inactivated complexes, MXenes efficiently adsorbed FAM fluorophore-modified single-stranded DNA (ssDNA-FAM), quenching its fluorescence. In presence AFB1, released due preferential binding aptamer then activated trans-cleavage activity indiscriminately cleave ssDNA MXenes, leading recovery signal. The fluorescent had wide range from 0.001 80 ng mL-1, limit 0.92 pg ability detect within min. More importantly, platform demonstrates excellent performance real peanut samples.

Язык: Английский

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Nanocellulose-based polymeric nanozyme as bioinspired spray coating for fruit preservation

Food Hydrocolloids, Год журнала: 2022, Номер 135, С. 108138 - 108138

Опубликована: Сен. 15, 2022

The growing concerns about microbial food safety and environmental contamination call for sustainable active packaging materials practices with high performance. This contribution proposed a biocatalytic spraying coating fruit preservation using carboxymethyl cellulose nanofibers grafting enzyme-like metal-organic frameworks ([email protected]) banana mango as model fruits. well-characterized biohybrid were verified to simultaneously mimic good oxidase-like apyrase-like activities, enabling effective sterilization against three typical foodborne pathogens, i.e. Escherichia coli O157: H7, Staphylococcus aureus, methicillin-resistant reaching killing rates 90.46%. biocidal •O2− generation adenosine triphosphate depletion the possible mechanisms causing nonspecific bacteria death. Such antibacterial performance, well additional protective properties of [email protected], facilitated quality during 12-day storage bananas mangos by spray biomimetic protected] It is expected that this work walks out first step nanozyme toward engineering fields food, agriculture, biomedicine.

Язык: Английский

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Metal–Organic Frameworks and Their Composites for Chronic Wound Healing: From Bench to Bedside

Advanced Materials, Год журнала: 2023, Номер 36(2)

Опубликована: Авг. 1, 2023

Chronic wounds are characterized by delayed and dysregulated healing processes. As such, they have emerged as an increasingly significant threat. The associated morbidity socioeconomic toll clinically financially challenging, necessitating novel approaches in the management of chronic wounds. Metal-organic frameworks (MOFs) innovative type porous coordination polymers, with low toxicity high eco-friendliness. Documented anti-bacterial effects pro-angiogenic activity predestine these nanomaterials promising systems for treatment In this context, therapeutic applicability efficacy MOFs remain to be elucidated. It is, therefore, reviewed structural-functional properties their composite materials discusses how multifunctionality customizability can leveraged a clinical therapy

Язык: Английский

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Mesoporous silica coated core-shell nanoparticles substrate for size-selective SERS detection of chloramphenicol

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2022, Номер 284, С. 121817 - 121817

Опубликована: Авг. 30, 2022

With the growing popularity of non-destructive technique, surface-enhanced Raman spectroscopy (SERS) demands a highly sensitive and reproducible plasmonic nanoparticles substrate. In this study, novel bimetallic core-shell (Au@Ag@mSiO2NP) substrate consisting gold core, silver shell, mesoporous silica coating was synthesized. The structure created by employing template molecules such as surfactant their subsequent removal allowing selective screening based on size analyte molecules. Results showed that could selectively enhance small preventing large macromolecules to reach exciting zone achieving detection chloramphenicol in milk samples with limit 6.68 × 10-8 M. Moreover, provided additional stability Au@Ag nanoparticles, leading reusability Thus, work offered simple smart Au@Ag@mSiO2NP for effective SERS analytes.

Язык: Английский

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Bio-interface engineering of MXene nanosheets with immobilized lysozyme for light-enhanced enzymatic inactivation of methicillin-resistant Staphylococcus aureus

Chemical Engineering Journal, Год журнала: 2022, Номер 452, С. 139078 - 139078

Опубликована: Сен. 8, 2022

Being a unique biocatalyst to hydrolyse structural polysaccharides on the cell wall of bacteria, lysozyme can slow down bacterial resistance caused by antibiotic overdose treatments, however, efficient activation activities under pathogenic microenvironments remains challenging. Herein, bio-interface engineering strategy was proposed for remote modulation thermoresistant upon rationally designed photothermal nanoplatforms. For this, Ti3C2TX MXene nanosheets were functionalized polydopamine (PDA) surface chemistry enhance effects and performance durability, during which biomacromolecules immobilized at such two-dimensional hybrid interface via intermolecular electrostatic affinity. The integrated nanoplatform (denoted as M@P@Lyso), with an optimal high light-to-heat conversion efficiency 46.88%, realized not only precision control local heat but also photo-responsive up-regulation bio-catalysis laden lysozyme. As result, in vitro vivo antibacterial experiments revealed that M@P@Lyso could effectively inhibit proliferation methicillin-resistant Staphylococcus aureus accelerated wound disinfection mice negligible biological toxicities. outstanding attributed photo-enhanced activity, assisted death mild hyperthermia physical destruction derived from M@PDA. This work exemplified solution threats stimuli-responsive enzymatic

Язык: Английский

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Tailoring the Surface and Composition of Nanozymes for Enhanced Bacterial Binding and Antibacterial Activity

Small, Год журнала: 2023, Номер 19(42)

Опубликована: Июнь 15, 2023

With the advantages of diverse structures, tunable enzymatic activity, and high stability, nanozymes are widely used in medicine, chemistry, food, environment, other fields. As an alternative to traditional antibiotics, attract more attention from scientific researchers recent years. Developing nanozymes-based antibacterial materials opens up a new avenue for bacterial disinfection sterilization. In this review, classification their mechanisms discussed. The surface composition critical efficacy, which can be tailored enhance both binding activity. On one hand, modification enables targeting bacteria that improves performance including biochemical recognition, charge, topography. modulated achieve enhanced single nanozyme-mediated synergistic multiple nanozymes-mediated cascade catalytic applications. addition, current challenges future prospects tailoring applications This review provide insights into design treatments.

Язык: Английский

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Tuning oxidant and antioxidant activities of ceria by anchoring copper single-site for antibacterial application

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 3, 2024

Abstract The reaction system of hydrogen peroxide (H 2 O ) catalyzed by nanozyme has a broad prospect in antibacterial treatment. However, the complex catalytic activities nanozymes lead to multiple pathways reacting parallel, causing uncertain results. New approach effectively regulate is urgent need. Herein, Cu single site modified on nanoceria with various activities, such as peroxidase-like activity (POD) and hydroxyl radical antioxidant capacity (HORAC). Benefiting from interaction between coordinated CeO substrate, POD enhanced while HORAC inhibited, which further confirmed density functional theory (DFT) calculations. Cu-CeO + H shows good properties both vitro vivo. In this work, strategy based metal carrier provides general clue for optimizing nanozymes.

Язык: Английский

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Visible detection of chilled beef freshness using a paper-based colourimetric sensor array combining with deep learning algorithms

Food Chemistry, Год журнала: 2024, Номер 441, С. 138344 - 138344

Опубликована: Янв. 6, 2024

Язык: Английский

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Fast real-time monitoring of meat freshness based on fluorescent sensing array and deep learning: From development to deployment

Food Chemistry, Год журнала: 2024, Номер 448, С. 139078 - 139078

Опубликована: Март 22, 2024

A fluorescent sensor array (FSA) combined with deep learning (DL) techniques was developed for meat freshness real-time monitoring from development to deployment. The made up of copper metal nanoclusters (CuNCs) and dyes, having a good ability in the quantitative qualitative detection ammonia, dimethylamine, trimethylamine gases low limit (as as 131.56 ppb) range 5 ∼ 1000 ppm visually various meats stored at 4 °C. Moreover, SqueezeNet applied automatically identify fresh level based on FSA images high accuracy (98.17 %) further deployed production environments such personal computers, mobile devices, websites by using open neural network exchange (ONNX) technique. entire recognition process only takes 7 s. Furthermore, gradient-weighted class activation mapping (Grad-CAM) uniform manifold approximation projection (UMAP) explanatory algorithms were used improve interpretability transparency SqueezeNet. Thus, this study shows new idea assisted DL intelligent

Язык: Английский

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