Abstract
Keratitis,
an
inflammation
of
the
cornea
caused
by
bacterial
or
fungal
infections,
is
one
leading
causes
severe
visual
disability
and
blindness.
Keratitis
treatment
requires
both
prevention
infection
reduction
inflammation.
However,
owing
to
their
limited
therapeutic
functions,
in
addition
ocular
barrier,
existing
conventional
medications
are
characterized
poor
efficacy
low
bioavailability,
requiring
high
dosages
frequent
topical
treatment,
which
represents
a
burden
on
patients
increases
risk
side
effects.
In
this
study,
manganese
oxide
nanocluster‐decorated
graphdiyne
nanosheets
(MnO
x
/GDY)
developed
as
multienzyme‐like
nanozymes
for
infectious
keratitis
loaded
into
hyaluronic
acid
polymethyl
methacrylate‐based
microneedles
(MGMN).
MGMN
not
only
exhibits
antimicrobial
anti‐inflammatory
effects
its
activities,
including
oxidase,
peroxidase,
catalase,
superoxide
dismutase
mimics
but
also
crosses
barrier
shows
increased
bioavailability
via
microneedle
system.
Moreover,
demonstrated
eliminate
pathogens,
prevent
biofilm
formation,
reduce
inflammation,
alleviate
hypoxia,
promote
repair
corneal
epithelial
damage
vitro,
ex
vivo,
vivo
experiments,
thus
providing
better
effect
than
commercial
ophthalmic
voriconazole,
with
no
obvious
microbial
resistance
cytotoxicity.
Advanced Materials,
Год журнала:
2023,
Номер
36(8)
Опубликована: Окт. 18, 2023
Abstract
Cascade
catalytic
reaction
exhibits
simple
procedure
and
high
efficiency,
such
as
that
from
the
orderly
assembly
of
different
enzymes
in
biological
systems.
Mimicking
natural
cascade
becomes
critical,
but
is
still
challenging.
Herein,
single
Au–Pt
nanozyme
reported
with
“three‐in‐one”
functions
to
initiate
conversions
for
O
2
supply
mimic
catalase,
H
production
its
glucose
oxidase‐like
property,
•
OH
generation
peroxidase
chemodynamic
therapy
(CDT).
Thus,
complex
cross‐talk
among
are
avoided.
To
this
end,
metastable
Cu
NPs,
scaffolds,
used
anchor
ultrasmall
nanozyme,
while
metal–organic
framework
(MOF)
encapsulate
tumor
microenvironment
response
shielding
protein
adsorption.
Pluronic
F127
then
modified
on
surface
improve
hydrophilicity
biocompatibility
composite.
The
endogenous
acidity
glutathione
degrade
MOF
expose
CDT.
photothermal
conversion
ability
also
enhances
CDT,
2+
ions
consume
GSH
further
CDT
efficiency
augmented
therapy.
a
new
paradigm
provided
drug‐free
improving
therapeutic
efficacy
minimizing
side
effects.
ACS Nano,
Год журнала:
2024,
Номер
18(4), С. 2533 - 2540
Опубликована: Янв. 12, 2024
Nanozymes,
nanomaterials
exhibiting
enzyme-like
activities,
have
emerged
as
a
prominent
interdisciplinary
field
over
the
past
decade.
To
date,
1200
different
been
identified
nanozymes,
covering
four
catalytic
categories:
oxidoreductases,
hydrolases,
isomerases,
and
lyases.
Catalytic
activity
specificity
are
two
pivotal
benchmarks
for
evaluating
enzymatic
performance.
Despite
substantial
progress
being
made
in
quantifying
optimizing
of
there
is
still
lack
in-depth
research
on
preventing
formation
consensual
knowledge
impeding
more
refined
systematic
classification
nanozymes.
Recently,
debates
regarding
whether
nanozymes
could
possess
similar
to
that
enzymes.
This
Perspective
discusses
by
referring
enzymes,
highlights
gap
between
concludes
offering
our
perspective
future
Abstract
Nanozymes
have
emerged
as
a
promising
alternative
to
natural
enzymes,
effectively
addressing
enzymes'
inherent
limitation.
Versatility
and
potential
applications
of
nanozyme
span
across
various
fields,
with
catalytic
tumor
therapy
being
one
prominent
area.
This
has
sparked
significant
interest
exploration
in
the
utilization
nanozymes
for
targeted
cancer
treatment.
Recent
advancements
interdisciplinary
research,
nanotechnology,
biotechnology,
technology
led
emergence
multi‐metallic‐based
nanozymes,
which
exhibit
tremendous
further
development.
review
focuses
on
investigating
synergistic
effects
aiming
enhance
our
understanding
their
activities
facilitate
broader
applications.
We
comprehensively
survey
remarkable
achievements
synthesis,
mechanisms,
latest
therapy.
Furthermore,
we
identify
current
limitations
prospects
development
new
materials
application
novel
technologies,
along
challenges
associated
underscores
significance
emphasizes
need
continued
well
impact
realization
breakthroughs
Advanced Materials,
Год журнала:
2023,
Номер
35(44)
Опубликована: Авг. 23, 2023
Inflammatory
bowel
disease
(IBD)
affects
millions
of
individuals
worldwide
annually.
Enteric
reactive
oxygen
species
(ROS)
play
critical
roles
in
the
physiology
and
pathology
IBD.
Nanozymes
hold
great
promise
for
treatment
IBD
because
their
exceptional
ability
to
regulate
redox
homeostasis
during
ROS-related
inflammation.
However,
rapid
development
orally
administered,
acid-tolerant,
antioxidant
nanozymes
therapy
is
challenging.
Here,
a
nine-tier
high-throughput
screening
strategy
established
address
multifaceted
demands,
including
intrinsic
stability,
radioactivity,
solubility,
gut
microbiome
toxicity,
biomimetic
elements,
intermediate
frontier
molecular
orbitals,
reaction
energy
barriers,
negative
charges,
acid
tolerance.
Ni3
S4
selected
as
best
matching
material
from
146
323
candidates,
which
exhibits
superoxide
dismutase-catalase
bienzyme-like
activity
3.13-
1.80-fold
more
active
than
natural
enzymes.
As
demonstrated
mouse
model,
stable
gastrointestinal
tract
without
toxicity
specifically
targets
diseased
colon
alleviate
oxidative
stress.
RNA
16S
rRNA
sequencing
analyses
show
that
effectively
inhibits
cellular
pathways
pro-inflammatory
factors
restores
microbiota.
This
study
not
develops
highly
efficient
administered
cascade
nanozyme
offers
next-generation
paradigm
rational
design
nanomedicine
through
data-driven
approaches.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(44), С. 16383 - 16391
Опубликована: Окт. 26, 2023
The
rational
design
of
efficient
nanozymes
and
the
immobilization
enzymes
are
great
significance
for
construction
high-performance
biosensors
based
on
nano-/bioenzyme
catalytic
systems.
Herein,
a
novel
V-TCPP(Fe)
metal-organic
framework
nanozyme
with
two-dimensional
nanosheet
morphology
is
rationally
designed
by
using
V2CTx
MXene
as
metal
source
iron
tetrakis(4-carboxyphenyl)porphine
(FeTCPP)
ligand
an
organic
linker.
It
exhibits
enhanced
peroxidase-
catalase-like
activities
luminol-H2O2
chemiluminescent
(CL)
behavior.
Based
experimental
theoretical
results,
these
excellent
enzyme-like
derived
from
two-site
synergistic
effect
between
V
nodes
FeTCPP
ligands
in
V-TCPP(Fe).
Furthermore,
confined
system
developed
zeolitic
imidazole
(ZIF)
coencapsulation
bioenzyme.
Using
acetylcholinesterase
(AChE)
model,
our
constructed
V-TCPP(Fe)/AChE@ZIF
was
successfully
used
colorimetric/CL
dual-mode
visual
biosensing
organophosphorus
pesticides.
This
work
expected
to
provide
new
insights
into
systems,
encouraging
applications
catalysis
biosensing.
Advanced Healthcare Materials,
Год журнала:
2023,
Номер
12(31)
Опубликована: Сен. 14, 2023
Abstract
Although
CeO
2
nanomaterials
have
been
widely
explored
as
nanozymes
for
catalytic
therapy,
they
still
suffer
from
relatively
low
activities.
Herein,
the
catalyzing
generation
and
stabilization
of
oxygen
vacancies
on
nanorods
by
Pt
nanoclusters
via
H
gas
reduction
under
mild
temperature
(350
°C)
to
obtain
Pt/CeO
2−
x
,
which
can
serve
a
highly
efficient
nanozyme
cancer
is
reported.
The
deposited
atomic
layer
deposition
technique
not
only
catalyst
generate
through
hydrogen
spillover
effect,
but
also
stabilize
generated
vacancies.
Meanwhile,
provide
anchoring
sites
forming
strong
metal‐support
interactions
thus
preventing
their
agglomerations.
Importantly,
reduced
at
350
°C
(Pt/CeO
‐350R)
exhibits
excellent
enzyme‐mimicking
activity
reactive
species
(e.g.,
·OH)
compared
other
control
samples,
including
temperatures,
achieving
performance
tumor‐specific
therapy
efficiently
eliminate
cells
in
vitro
ablate
tumors
vivo.
‐350R
originates
good
activities
vacancy‐rich
nanoclusters.
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(11), С. 7565 - 7574
Опубликована: Март 6, 2024
Multienzyme-like
nanozymes
are
nanomaterials
with
multiple
enzyme-like
activities
and
the
focus
of
nanozyme
research
owing
to
their
ability
facilitate
cascaded
reactions,
leverage
synergistic
effects,
exhibit
environmentally
responsive
selectivity.
However,
multienzyme-like
varying
under
different
conditions,
making
them
difficult
precisely
regulate
according
design
requirements.
Moreover,
individual
activity
in
a
may
accelerate,
compete,
or
antagonize
each
other,
rendering
overall
complex
interplay
these
factors
rather
than
simple
sum
single
activity.
A
theoretically
guided
strategy
is
highly
desired
accelerate
nanozymes.
Herein,
information
was
collected
from
4159
publications
build
database
covering
element
type,
ratio,
chemical
valence,
shape,
pH,
etc.
Based
on
clustering
correlation
coefficients
information,
material
features
distinct
classifications
were
reorganized
generate
compositional
for
advanced
methods
developed,
including
quantum
mechanics/molecular
mechanics
method
analyzing
surface
adsorption
binding
energies
substrates,
transition
states,
products
reaction
pathways,
along
machine
learning
algorithms
identify
optimal
pathway,
aid
evolutionary
This
approach
culminated
creating
CuMnCo7O12,
active
nanozyme.
process
named
genetic-like
because
it
resembles
biological
genetic
evolution
nature
offers
feasible
protocol
theoretical
foundation
constructing
Nano-Micro Letters,
Год журнала:
2024,
Номер
16(1)
Опубликована: Фев. 6, 2024
Inflammatory
skin
disorders
can
cause
chronic
scarring
and
functional
impairments,
posing
a
significant
burden
on
patients
the
healthcare
system.
Conventional
therapies,
such
as
corticosteroids
nonsteroidal
anti-inflammatory
drugs,
are
limited
in
efficacy
associated
with
adverse
effects.
Recently,
nanozyme
(NZ)-based
hydrogels
have
shown
great
promise
addressing
these
challenges.
NZ-based
possess
unique
therapeutic
abilities
by
combining
benefits
of
redox
nanomaterials
enzymatic
activity
water-retaining
capacity
hydrogels.
The
multifaceted
effects
include
scavenging
reactive
oxygen
species
other
inflammatory
mediators
modulating
immune
responses
toward
pro-regenerative
environment
enhancing
regenerative
potential
triggering
cell
migration
differentiation.
This
review
highlights
current
state
art
NZ-engineered
(NZ@hydrogels)
for
regeneration
applications.
It
also
discusses
underlying
chemo-mechano-biological
mechanisms
behind
their
effectiveness.
Additionally,
challenges
future
directions
this
ground,
particularly
clinical
translation,
addressed.
insights
provided
aid
design
engineering
novel
hydrogels,
offering
new
possibilities
targeted
personalized
skin-care
therapies.
