Multienzyme‐Like Nanozyme Encapsulated Ocular Microneedles for Keratitis Treatment

Small, Год журнала: 2023, Номер 20(21)

Опубликована: Дек. 15, 2023

Abstract Keratitis, an inflammation of the cornea caused by bacterial or fungal infections, is one leading causes severe visual disability and blindness. Keratitis treatment requires both prevention infection reduction inflammation. However, owing to their limited therapeutic functions, in addition ocular barrier, existing conventional medications are characterized poor efficacy low bioavailability, requiring high dosages frequent topical treatment, which represents a burden on patients increases risk side effects. In this study, manganese oxide nanocluster‐decorated graphdiyne nanosheets (MnO x /GDY) developed as multienzyme‐like nanozymes for infectious keratitis loaded into hyaluronic acid polymethyl methacrylate‐based microneedles (MGMN). MGMN not only exhibits antimicrobial anti‐inflammatory effects its activities, including oxidase, peroxidase, catalase, superoxide dismutase mimics but also crosses barrier shows increased bioavailability via microneedle system. Moreover, demonstrated eliminate pathogens, prevent biofilm formation, reduce inflammation, alleviate hypoxia, promote repair corneal epithelial damage vitro, ex vivo, vivo experiments, thus providing better effect than commercial ophthalmic voriconazole, with no obvious microbial resistance cytotoxicity.

Язык: Английский

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Natural Metal Polyphenol Nanozyme: Free Radical Scavenging and Antioxidation for the Treatment of Acute Kidney Injury

ACS Materials Letters, Год журнала: 2023, Номер 5(10), С. 2807 - 2819

Опубликована: Сен. 14, 2023

Acute kidney injury (AKI) is a severe renal dysfunction syndrome associated with high morbidity and mortality. Excessive production of reactive oxygen/nitrogen species (RONS) involved in the development progression AKI. Therefore, scavenging endogenous RONS reducing excessive oxidative stress essential for restoring function after Although nanozyme-mediated antioxidant therapy has been proposed as an effective strategy to prevent AKI, low uptake organ toxicity hinder its potential practical applications. To address this problem, we have designed metal-phenolic nanozyme named RosA-Mn NPs, which possesses cascade functionalities. The multienzymatic capacity well broad spectrum ROS free radical activities. As expected, NPs promote recovery mitochondrial reduce endoplasmic reticulum secondary inflammation through vitro. Moreover, overcome shortcomings artificial enzymes that failed rapidly accumulate tissues. tubular targeting could be exploited mitigate damage, improve functions, eventually Notably, also protect from oxidation cisplatin-induced AKI mouse model. RNA sequencing analysis demonstrated autophagy, apoptosis, antioxidation-related pathways were modulated by treatment NPs. In summary, activities biocompatibility show great other ROS-related diseases.

Язык: Английский

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Au/Pt@ZIF-90 Nanoenzyme Capsule-Based “Explosive” Signal Amplifier for “All-in-Tube” POCT

Analytical Chemistry, Год журнала: 2024, Номер 96(3), С. 1362 - 1370

Опубликована: Янв. 10, 2024

The sensitive, convenient, and visual detection of low-concentration disease markers in biological samples has always been a priority diagnosis. However, existing research problematic due to complex operation unsatisfactory sensitivity. Consequently, an "explosive" signal amplification platform based on Au/Pt@ZIF-90 was developed for sensitive markers. In this study, controllable explosively released Au/Pt nanoparticles (NPs) "nanoenzyme capsule" prepared by encapsulating NPs with excellent peroxidase activity ZIF-90. This achieved adjusting the particle size ZIF-90 encapsulation amount NPs. Using capsules as output module aptamer target recognition module, "All-in-Tube" portable point-of-care (POC) constructed integrating Au/Pt@ZIF-90/filter paper TMB/strips into Eppendorf (EP) tube. By utilizing specific competitive binding targets aptamers, enabled convenient measurement small molecular Taking adenosine proof concept, Moreover, can achieve universal various varying sequence. strategy provides design pattern holds significant potential fields diagnosis environmental monitoring.

Язык: Английский

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Space-confined nanozyme with cascade reaction based on PCN-224 for synergistic bacterial infection treatment and NIR fluorescence imaging of wound bacterial infections

Chemical Engineering Journal, Год журнала: 2024, Номер 487, С. 150642 - 150642

Опубликована: Март 26, 2024

Язык: Английский

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Neuromodulation by nanozymes and ultrasound during Alzheimer’s disease management

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Март 30, 2024

Язык: Английский

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Iridium nanoparticles supported on polyaniline nanotubes for peroxidase mimicking towards total antioxidant capacity assay of fruits and vegetables

Food Chemistry, Год журнала: 2024, Номер 445, С. 138732 - 138732

Опубликована: Фев. 13, 2024

Язык: Английский

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Core–Shell Structured Nanozyme with PDA-Mediated Enhanced Antioxidant Efficiency to Treat Early Intervertebral Disc Degeneration

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(4), С. 5103 - 5119

Опубликована: Янв. 17, 2024

Early intervention during intervertebral disc degeneration (IDD) plays a vital role in inhibiting its deterioration and activating the regenerative process. Aiming at high oxidative stress (OS) IDD microenvironment, core–shell structured nanozyme composed of Co-doped NiO nanoparticle (CNO) as core encapsulated with polydopamine (PDA) shell, named PDA@CNO, was constructed, hoping to regulate pathological environment. The results indicated that coexistence abundant Ni3+/Ni2+and Co3+/Co2+redox couples CNO provided rich catalytic sites; meanwhile, quinone catechol groups PDA shell could enable proton-coupled electron transfer, thus endowing PDA@CNO multiple antioxidative enzyme-like activities scavenge •O2–, H2O2, •OH efficiently. Under OS conditions vitro, effectively reduce intracellular ROS nucleus pulposus (NP) into friendly H2O O2, protect NP cells from stagnant proliferation, abnormal metabolism (senescence, mitochondria dysfunction, impaired redox homeostasis), inflammation, thereby reconstructing extracellular matrix (ECM) homeostasis. vivo local injection experiments further proved desirable therapeutic effects rat model, suggesting great potential prohibiting deterioration.

Язык: Английский

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Dual-mode sensing platform based on aptamer-tunable catalytic activity of mesoporous polydopamine/MnO2 nanozymes for detecting S. aureus

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 393, С. 134218 - 134218

Опубликована: Июнь 28, 2023

Язык: Английский

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Single-atom nanozymes as promising catalysts for biosensing and biomedical applications

Inorganic Chemistry Frontiers, Год журнала: 2023, Номер 10(15), С. 4289 - 4312

Опубликована: Янв. 1, 2023

Single-atom nanozymes represent a unique class of enzyme mimics that exhibit maximal atomic utilization, well-defined electronic/geometric structure, and high catalytic activity, have found diverse applications in biomedicine biosensing.

Язык: Английский

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Nanozyme-based cancer theranostics: A scientometric analysis and comprehensive review

Nano Today, Год журнала: 2024, Номер 57, С. 102386 - 102386

Опубликована: Июль 13, 2024

Язык: Английский

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