Highly
sensitive
lateral
flow
immunoassays
(LFIAs)
are
essential
for
various
point-of-care
applications,
and
gold
nanoparticles
(Au
NPs)
by
far
the
most
commonly
used
labels.
However,
conventional
LFIAs
often
suffer
from
high
detection
limits
(LOD)
or
low
sensitivity.
In
this
study,
we
investigated
three
strategies
to
enhance
sensitivity
of
improving
peroxidase-mimicking
(POD)
activity
Au
NPs.
The
POD
unmodified
NPs
was
negligible
(<0.01
units/mg,
U/mg).
first
strategy
involved
coupling
with
horseradish
peroxidase
(HRP),
which
increased
65
U/mg.
second
approach
forming
a
thin
palladium
iridium
shell
on
NPs,
elevated
0.69-0.71
third
binding
mercury
ions
(Hg2+)
resulting
in
up
3
Finally,
developed
simple
quantitative
model
estimate
LOD
based
kinetic
parameters.
Using
Au-HRP
conjugates,
demonstrated
that
experimentally
measured
consistent
calculated
values.
provides
framework
evaluating
catalytic
signal
amplification
can
be
guide
development
highly
assays.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Сен. 16, 2024
Nanozymes
have
been
attracting
widespread
interest
for
the
past
decade,
especially
in
field
of
cancer
therapy,
due
to
their
intrinsic
catalytic
activities,
strong
stability,
and
ease
synthesis.
However,
enhancing
activity
tumor
microenvironment
(TME)
remains
a
major
challenge.
Herein,
we
manipulate
activities
Ru
nanozymes
via
modulating
lattice
spacing
nanocrystals
supported
on
nitrogen-doped
carbon
support,
achieve
improvement
multiple
enzyme-like
that
can
form
cascade
reactions
boost
cell
killing.
In
addition,
expansion
improve
responsiveness
self-powered
electric
field,
achieving
maximized
therapeutic
outcome.
Under
electrical
stimulation
provided
by
human
self-propelled
triboelectric
device,
Ru-based
nanozyme
(Ru1000)
with
5.99%
realizes
optimal
performance
outcome
breast
female
tumor-bearing
mice.
Through
theoretical
calculations,
uncover
promote
reaction,
simultaneously,
reducing
electron
density
shifting
d-band
center
active
sites.
This
work
provides
opportunities
improving
development
nanozymes.
Abstract
Acute
pancreatitis
(AP)
is
a
potentially
life‐threatening
inflammatory
disease
that
can
lead
to
the
development
of
systemic
response
syndrome
and
its
progression
severe
acute
pancreatitis.
Hence,
there
an
urgent
need
for
rational
design
highly
efficient
antioxidants
treat
AP.
Herein,
optimized
Cu‐based
metal–organic
framework
(MOF)
nanozyme
with
exceptional
antioxidant
activity
introduced,
designed
effectively
alleviate
AP,
by
engineering
metal
coordination
centers
in
MN2Cl2
(M
=
Co,
Ni,
Cu).
Specifically,
Cu
MOF,
which
benefits
from
active
center
similar
natural
superoxide
dismutase
(SOD),
exhibited
at
least
four
times
higher
SOD‐like
than
Ni/Co
MOF.
Theoretical
analyses
further
demonstrate
CuN2Cl2
site
not
only
has
moderate
adsorption
effect
on
substrate
molecule
•OOH
but
also
reduces
dissociation
energy
product
H
2
O
.
Additionally,
MOF
possesses
excellent
catalase‐like
•OH
removal
ability.
Consequently,
broad‐spectrum
efficiently
scavenge
reactive
oxygen
species
arginine‐induced
More
importantly,
it
mitigate
apoptosis
necrosis
acinar
cells
activating
PINK1/PARK2‐mediated
mitophagy
pathway.
This
study
highlights
distinctive
functions
tunable
nanozymes
their
potential
bio‐applications.
Abstract
The
clinical
application
of
oncology
therapy
is
hampered
by
high
glutathione
concentrations,
hypoxia,
and
inefficient
activation
cell
death
mechanisms
in
cancer
cells.
In
this
study,
Fe
Mo
bimetallic
sulfide
nanomaterial
(FeS
2
@MoS
)
based
on
metal‐organic
framework
structure
rationally
prepared
with
peroxidase
(POD)‐,
catalase
(CAT)‐,
superoxide
dismutase
(SOD)‐like
activities
depletion
ability,
which
can
confer
versatility
for
treating
tumors
mending
wounds.
the
lesion
area,
FeS
SOD‐like
activity
facilitate
transformation
anions
(O
−
to
hydrogen
peroxide
(H
O
),
then
resulting
H
serves
as
a
substrate
Fenton
reaction
FMS
produce
highly
toxic
hydroxyl
radicals
(
∙
OH).
Simultaneously,
has
an
ability
deplete
(GSH)
catalyze
decomposition
nicotinamide
adenine
dinucleotide
phosphate
(NADPH)
curb
regeneration
GSH
from
source.
Thus
it
realize
effective
tumor
elimination
through
synergistic
apoptosis‐ferroptosis
strategy.
Based
alteration
system,
free
radical
production,
alleviation
hypoxia
microenvironment,
NPS
not
only
significantly
inhibit
vivo
vitro,
but
also
multidrug‐resistant
bacteria
hasten
wound
healing.
It
may
open
door
development
cascade
nanoplatforms
treatment
overcoming
infection.
Biosensors,
Год журнала:
2023,
Номер
13(8), С. 801 - 801
Опубликована: Авг. 10, 2023
The
colorimetric
signal
readout
method
is
widely
used
in
visualized
analyses
for
its
advantages,
including
visualization
of
test
results,
simple
and
fast
operations,
low
detection
cost
response
time.
Gold
nanoparticles
(Au
NPs),
which
not
only
exhibit
enzyme-like
activity
but
also
have
the
advantages
tunable
localized
surface
plasmon
resonance
(LSPR),
high
stability,
good
biocompatibility
easily
modified
properties,
provide
excellent
platforms
construction
sensors.
They
are
environmental
monitoring,
biomedicine,
food
industry
other
fields.
This
review
focuses
on
chromogenic
mechanisms
sensors
based
Au
NPs
adopting
two
different
sensing
strategies
summarizes
significant
advances
NP-based
with
LSPR
characteristics.
In
addition,
properties
classified
into
four
modulation
methods:
aggregation,
modification,
deposition
etching,
current
status
visual
various
analytes
discussed.
Finally,
further
discusses
limitations
promising
prospects
as
sensors,
guiding
design
novel
ACS Applied Nano Materials,
Год журнала:
2023,
Номер
6(24), С. 23303 - 23312
Опубликована: Дек. 5, 2023
Developing
peroxidase-like
sensors
with
excellent
catalytic
activity
to
construct
colorimetric
has
been
an
important
challenge
for
a
long
time.
The
electronegativity
of
the
doped
nonmetallic
atoms
can
change
chemical
environment
carbon
material
and
promote
redistribution
electrons,
thus
improving
electron
transfer
channel
between
nanozyme
reaction
substrate
further
enhancing
activity.
Herein,
nitrogen
(N),
phosphorus
(P),
sulfur
(S)
codoped
(NPS-C)
was
successfully
designed
via
one-step
high-temperature
pyrolysis.
Compared
N-doped
nanozymes
(N–C),
N
P
(NP-C)
have
lower
Michaelis–Menten
constant,
S
(NS-C)
higher
rate.
Therefore,
based
on
synergy
different
atoms,
NPS-C
exhibited
stronger
binding
affinity,
which
effectively
activated
H2O2
produce
reactive
oxygen
intermediates
oxidation
capacity
superoxide
anion
radical
(•O2–)
promoted
TMB
lose
be
oxidized
oxTMB.
Moreover,
ascorbic
acid
(AA),
as
antioxidant
model,
rapidly
consume
species
reduce
linear
relationship
concentration
AA
inhibition
degree
activity,
sensor
constructed
analyze
total
(TAC)
in
beverages.
