Опубликована: Янв. 1, 2024
Язык: Английский
Carbohydrate Polymers, Год журнала: 2024, Номер unknown, С. 121812 - 121812
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
46
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 11
Опубликована: Янв. 8, 2024
Introduction: Intrauterine adhesions (IUA), also known as Asherman's syndrome, is caused by trauma to the pregnant or non-pregnant uterus, which leads damaged endometrial basal lining and partial total occlusion of uterine chambers, resulting in abnormal menstruation, infertility, recurrent miscarriage. The essence this syndrome fibrosis. And there no effective treatment for IUA stimulate regeneration currently. Recently, menstrual blood-derived stem cells (MenSCs) have been proved hold therapeutic promise various diseases, such myocardial infarction, stroke, diabetes, liver cirrhosis. Methods: In study, we examined effects MenSCs on repair a rat model, more importantly, promoted via xeno-free VitroGel MMP carrier. Results: This combined reduced expression inflammatory factors, increased anti-inflammatory restricted area fibrosis, diminished adhesions, partially restored fertility, showing stronger effectiveness than each component alone almost resembling sham group. Discussion: Our findings suggest highly promising strategy treatment.
Язык: Английский
Процитировано
5
Advanced Science, Год журнала: 2024, Номер 11(26)
Опубликована: Апрель 30, 2024
Abstract Easy injection, long‐lasting barrier, and drug loading are the critical properties of submucosal injection materials for endoscopic surgery. However, conventional injectable polymers face challenges in simultaneously attaining these due to inherent conflict between injectability situ stability. Here, a multi‐arm star polymer hydrogel (denoted as βCP hydrogel) with barrier (exceeding 120 min), rapid hemostasis, sustained antibacterial is successfully developed by grafting poly(oligo(ethylene glycol) methyl ether methacrylate) (PEGMA) side‐chains from β‐CD via atom transfer radical polymerization (ATRP). During onset shearing, experiences unwinding neighboring polymers, which facilitates process injectability. After undergoes winding side‐chains, thereby establishing cushion. Meanwhile, owing its distinctive structures hydrophobic inner cavity an outer layer hydrophilic enables simultaneous on‐demand release diverse categories drugs. This unique performance can adapt demands during different stages wound healing porcine surgery model. These results indicate appealing prospect new application good material treatments.
Язык: Английский
Процитировано
4
Small, Год журнала: 2024, Номер unknown
Опубликована: Сен. 28, 2024
Abstract Postoperative adhesion (POA) is a common and serious complication following various types of surgery. Current physical barriers either have short residence time at the surgical site with low tissue attachment capacity or are prone to undesired formation owing double‐sided adhesive property, which limits POA prevention efficacy barriers. In this study, Janus‐structured microgels (Janus‐MGs) asymmetric capabilities fabricated using novel bio‐friendly gas‐shearing microfluidic platform. The anti‐adhesive side Janus‐MGs, consists alginate, hyaluronic acid, derivatives, endows material separation, lubrication, properties. provided Janus‐MGs retention capability through catechol‐based adhesion, thereby enhancing in situ effect. addition, significantly reduced blood loss shortened hemostatic rats, further reducing formation. Three commonly used rat models different structures motion patterns established namely peritoneal intrauterine peritendinous models, results showed that effectively prevented occurrence all models. fabrication offers reliable strategy promising paradigm for preventing diverse procedures.
Язык: Английский
Процитировано
4
Reproductive Biology and Endocrinology, Год журнала: 2025, Номер 23(1)
Опубликована: Янв. 17, 2025
Preimplantation embryos in vivo are exposed to various growth factors the female reproductive tract that absent vitro embryo culture media. Cell-free fat extract exerts antioxidant, anti-ageing, and ovarian function-promoting effects. However, its effects on quality yet be investigated. We assessed effect of cell-free supplementation using a naturally ageing mouse model. model's efficacy influencing development pregnancy rates older women with fertilization failure. In addition, we performed immunofluorescence staining, multiplex immunoassay, whole-genome amplification DNA sequencing, time-lapse monitoring, experiments. extract-supplemented media has suitable osmolarity pH contains high levels bioactive factors. promoted implantation aged mice, probably by increasing rate, inhibiting cell apoptosis, promoting blastocyst adhesion. Clinical results showed group had significantly higher day 3 available high-quality than control group, rate usable tended group. Furthermore, clinical improved Our study implies can promote outcomes may serve as rescue strategy for
Язык: Английский
Процитировано
0
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Янв. 17, 2025
Regeneration of the injured endometrium, particularly functional layer, is crucial for prevention uterine infertility. At present, clinical treatment using sodium hyaluronate hydrogel injection limited by its relatively low fluidity, short-term retention, and insufficient bioactive ingredients, so it necessary to develop an advanced healing-promoting hydrogel. The modulation microenvironment Lactobacillus presents a component that can facilitate regeneration layer. Our study introduces multifunctional Lactobacillus-loaded poly(N-isopropylacrylamide)-grafted bacterial cellulose (BC-g-PN@L) designed with superior injectability in situ stability. 25 °C (room temperature), uniform distribution achieved pressure only 7.90 kPa. 37 (body BC-g-PN@L forms robust three-dimensional nanonetwork, providing space substance exchange channels maintain viability bioactivity. Enhanced hydrophobic isopropyl groups poly(N-isopropylacrylamide) side chains rigid substrates, exhibits prolonged retention properties cavity, persisting over 21 days. These attributes endow versatile pro-healing capacity rat model endometrial injury. promotes development injectable hydrogels both histological repair endometrium.
Язык: Английский
Процитировано
0
Journal of Bioactive and Compatible Polymers, Год журнала: 2025, Номер unknown
Опубликована: Фев. 13, 2025
The treatment of large segmental bone defects has always been a difficult problem in orthopedic treatment, which is characterized by local microenvironment with impaired blood supply and absolute or relatively insufficient osteogenic ability. In these clinical scenarios, the repair process often hindered, incidence complications increased. Therefore, obtaining coordinated angiogenesis osteogenesis will be an effective strategy. this study, we incorporated ophiopogon D (OPD) into hydrogel matrix network amino polyethylene glycol sodium alginate oxide as raw materials to construct injectable functional biomaterial for promoting differentiation. vitro studies showed that constructed material had satisfactory injectability self-healing properties, could continuously release OPD locally, thus migration human umbilical vein endothelial cells vitro, differentiation marrow mesenchymal stem cells. After was implanted defect radius rabbit model, it enhance expression angiogenic protein, improve microenvironment, accelerate repair. This study provides new strategy theoretical basis design minimally invasive tissue engineering defects.
Язык: Английский
Процитировано
0
Materials Today Bio, Год журнала: 2024, Номер 26, С. 101045 - 101045
Опубликована: Апрель 3, 2024
The endometrium undergoes a series of precise monthly changes under the regulation dynamic levels ovarian hormones that are characterized by repeated shedding and subsequent regeneration without scarring. This provides potential for wound healing during endometrial injuries. Bioengineering materials highlight faithful replication constitutive cells extracellular matrix simulates physical biomechanical properties to larger extent. Significant progress has been made in this field, functional tissue bioengineering allows an in-depth investigation regulatory factors myometrial defects vitro highly therapeutic methods alleviate obstetric gynecological complications. However, much remains be learned about latest application technologies human endometrium. Here, we summarize existing developments biomaterials models improving female reproductive potential.
Язык: Английский
Процитировано
3
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 8285 - 8308
Опубликована: Авг. 1, 2024
The endometrium is an extremely important component of the uterus and crucial for individual health human reproduction.However, traditional methods still struggle to ideally repair structure function damaged restore fertility.Therefore, seeking developing innovative technologies materials has potential regenerate or diseased endometrium.The emergence functionalization various nanomedicine biomaterials, as well proposal development regenerative medicine tissue engineering techniques, have brought great hope solving these problems.In this review, we will summarize nanomedicine, that contribute endometrial regeneration, including nanoscale exosomes, nanomaterials, stem cell-based materials, naturally sourced chemically synthesized approaches functionalizing application revolutionary new such organoids, organ-on-chips, artificial intelligence, etc.The diverse design modification biomaterials endow them with functionalities, microstructure nanostructure, mechanical properties, biological functions, cellular microenvironment regulation.It provide options regeneration endometrium, bring reconstruction recovery patients' reproductive abilities.
Язык: Английский
Процитировано
3
Materials Today Bio, Год журнала: 2023, Номер 23, С. 100857 - 100857
Опубликована: Ноя. 15, 2023
Endometrium is suspectable to severe injury due recurrent abortion, curettage or intrauterine infection which could lead pathological conditions and sabotage women's fertility. Promoting endometrium regeneration the core of treatments uterine related infertility. Patients who received traditional can only expect limited effects, thereby novel therapies are badly in need promote regeneration. Here we generated a decellularized extracellular matrix (ECM) from porcine dermis, composited adipose stem cell derived exosomes (ADSC-exos) on it (ECM@ADSC-exos). In vitro experiments proved that ECM@ADSC-exos exhibited good cytocompatibility improve proliferation, migration angiogenesis. We also observed that, when implanted cavity rat model injury, improved regeneration, enhanced local angiogenesis, promoted myometrium repair finally preserved Our results be option for
Язык: Английский
Процитировано
7