Protein Corona of Nanoparticles: Isolation and Analysis DOI Creative Commons

Yinuo Sun,

Yaxin Zhou, Mubashar Rehman

и другие.

Chem & Bio Engineering, Год журнала: 2024, Номер 1(9), С. 757 - 772

Опубликована: Окт. 3, 2024

Nanoparticles entering biological systems or fluids inevitably adsorb biomolecules, such as protein, on their surfaces, forming a protein corona. Ensuing, the corona endows nanoparticles with new identity and impacts interaction between systems. Hence, development of reliable techniques for isolation analysis is key understanding behaviors nanoparticles. First, this review systematically outlines approach isolating corona, including centrifugation, magnetic separation, size exclusion chromatography, flow-field-flow fractionation, other emerging methods. Next, we qualitative quantitative characterization methods Finally, underscore necessary steps to advance efficiency fidelity nanoparticle surfaces. We anticipate that these insights into methodologies will profoundly influence technologies aimed at elucidating bionano interactions role in various biomedical applications.

Язык: Английский

Advances and applications of nanoparticles in cancer therapy DOI Creative Commons

Xianzhou Huang,

Tao He, Xiuqi Liang

и другие.

MedComm – Oncology, Год журнала: 2024, Номер 3(1)

Опубликована: Март 1, 2024

Abstract Rapid growth in nanoparticles (NPs) as delivery systems holds vast promise to promote therapeutic approaches for cancer treatment. Presently, a diverse array of NPs with unique properties have been developed overcome different challenges and achieve sophisticated routes enhancement series therapies. Inspiring advances achieved the field therapy using NPs. In this review, we aim summarize up‐to‐date progression addressing various challenges, expect elicit novel potential opportunities alternatively. We first introduce sorts NP technologies, illustrate their mechanisms, present applications. Then, achievements made by break obstacles delivering cargoes specific sites through particular are highlighted, including long‐circulation, tumor targeting, responsive release, subcellular localization. subsequently retrospect recent research treatments from single therapy, like chemotherapy, combination chemoradiotherapy, integrative therapy. Finally, perspectives impact on oncology discussed. believe review can offer deeper understanding

Язык: Английский

Процитировано

31
Concepts and Approaches to Reduce or Avoid Protein Corona Formation on Nanoparticles: Challenges and Opportunities DOI Creative Commons
Matthias Barz, Wolfgang J. Parak, Rudolf Zentel

и другие.

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Июль 8, 2024

This review describes the formation of a protein corona (or its absence) on different classes nanoparticles, basic principles, and consequences for nanomedicine. For this purpose, it general concepts to control (guide/minimize) interaction between artificial nanoparticles plasma proteins reduce formation. Thereafter, methods qualitative or quantitative determination are presented, as well properties nanoparticle surfaces, which relevant prevention formation). Thereby especially role grafting density hydrophilic polymers surface is discussed prevent corona. In context also potential detergents (surfactants) temporary modification grafting-to grafting-from approaches permanent discussed. The concludes by highlighting several promising avenues. includes (i) use without active targeting, (ii) synthetic address immune system, (iii) recollection with defined after in vivo application sample blood proteome (iv) further

Язык: Английский

Процитировано

22
Applications of liposomes and lipid nanoparticles in cancer therapy: current advances and prospects DOI Creative Commons
Zhe Cheng, Huichao Huang, Mingru Yin

и другие.

Experimental Hematology and Oncology, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 31, 2025

Liposomes and lipid nanoparticles are common lipid-based drug delivery systems play important roles in cancer treatment vaccine manufacture. Although significant progress has been made with these nanocarriers recent years, efficient clinical translation of active targeted liposomal remains extremely challenging. In this review, we focus on liposomes, stimuli-responsive strategy combined therapy treatment. We also summarize advances liposome nanoparticle applications nucleic acid tumor vaccination. addition, discuss limitations challenges the nanomaterials make recommendations for future research therapy.

Язык: Английский

Процитировано

5
AI-Based Prediction of Protein Corona Composition on DNA Nanostructures DOI Creative Commons
Jared Huzar, Roxana Coreas, Markita P. Landry

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

DNA nanotechnology has emerged as a powerful approach to engineering biophysical tools, therapeutics, and diagnostics because it enables the construction of designer nanoscale structures with high programmability. Based on base pairing rules, nanostructure size, shape, surface functionality, structural reconfiguration can be programmed degree spatial, temporal, energetic precision that is difficult achieve other methods. However, properties structure constructs are greatly altered in vivo due spontaneous protein adsorption from biofluids. These adsorbed proteins, referred corona, remain challenging control or predict, subsequently, their functionality fate engineer. To address these challenges, we prepared library diverse nanostructures investigated relationship between design features composition corona. We identified characteristics important for developed machine-learning model predicts which proteins will enriched based structures' properties. Our work help understand program function biomedical applications.

Язык: Английский

Процитировано

2
Identification of the Proteins Determining the Blood Circulation Time of Nanoparticles DOI

Cintia Marques,

Mohammad Javad Hajipour, Célia Marets

и другие.

ACS Nano, Год журнала: 2023, Номер 17(13), С. 12458 - 12470

Опубликована: Июнь 28, 2023

The therapeutic efficacy and adverse impacts of nanoparticles (NPs) are strongly dependent on their systemic circulation time. corona proteins adsorbed the NPs determine plasma half-lives, hence, it is crucial to identify shortening or extending In this work, in vivo time composition superparamagnetic iron oxide (SPIONs) with different surface charges/chemistries were analyzed over SPIONs neutral positive charges showed longest shortest times, respectively. most striking observation was that corona-coated similar opsonin/dysopsonin content implying these biomolecules not only contributing factors. Long-circulating adsorb higher concentrations osteopontin, lipoprotein lipase, coagulation factor VII, matrix Gla protein, secreted phosphoprotein 24, alpha-2-HS-glycoprotein, apolipoprotein C-I, while short-circulating amounts hemoglobin. Therefore, may be considered determining factors governing NP

Язык: Английский

Процитировано

30
Recent advances and prospects for lipid-based nanoparticles as drug carriers in the treatment of human retinal diseases DOI Creative Commons
Shaoxue Zeng, Yingying Chen, Fanfan Zhou

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 199, С. 114965 - 114965

Опубликована: Июнь 13, 2023

The delivery of cures for retinal diseases remains problematic. There are four main challenges: passing through multiple barriers the eye, to particular cell types, capability carry different forms therapeutic cargo and long-term efficacy. Lipid-based nanoparticles (LBNPs) potent overcome these challenges due their unique merits: amphiphilic nanoarchitectures pass biological barriers, vary modifications with specific affinity target flexible capacity large mixed types cargos slow-release formulations treatment. We have reviewed latest research on applications LBNPs treating categorized them by payloads. Furthermore, we identified technical discussed possible future development expand potential in diseases.

Язык: Английский

Процитировано

24
Characterization of the effects of bridging linker on the β-Lactoglobulin binding mechanism on the nanoscale metal-organic frameworks DOI
Na Gan, Yali Song,

Yilin Li

и другие.

Food Chemistry, Год журнала: 2024, Номер 464, С. 141715 - 141715

Опубликована: Окт. 19, 2024

Язык: Английский

Процитировано

15
A uniform data processing pipeline enables harmonized nanoparticle protein corona analysis across proteomics core facilities DOI Creative Commons
Hassan Gharibi, Ali Akbar Ashkarran, Maryam Jafari

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 6, 2024

Abstract Protein corona, a layer of biomolecules primarily comprising proteins, forms dynamically on nanoparticles in biological fluids and is crucial for predicting nanomedicine safety efficacy. The protein composition the corona typically analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). Our recent study, involving identical samples by 17 proteomics facilities, highlighted significant data variability, with only 1.8% proteins consistently identified across these centers. Here, we implement an aggregated database search unifying parameters such as variable modifications, enzyme specificity, number allowed missed cleavages stringent 1% false discovery rate at peptide levels. Such uniform dramatically harmonizes data, increasing reproducibility percentage consistency-identified unique distinct cores. Specifically, out 717 quantified 253 (35.3%) are shared among top 5 facilities (and 16.2% 11 facilities). Furthermore, note that reduction alkylation important steps sample processing expected, omitting reduces total peptides around 20%. These findings underscore need standardized procedures analysis, which vital advancing clinical applications nanoscale biotechnologies.

Язык: Английский

Процитировано

13
Critical Review: Uptake and Translocation of Organic Nanodelivery Vehicles in Plants DOI
Luiza Stolte Bezerra Lisboa Oliveira, Kurt D. Ristroph

Environmental Science & Technology, Год журнала: 2024, Номер 58(13), С. 5646 - 5669

Опубликована: Март 22, 2024

Nanodelivery vehicles (NDVs) are engineered nanomaterials (ENMs) that, within the agricultural sector, have been investigated for their ability to improve uptake and translocation of agrochemicals, control release, or target specific tissues subcellular compartments. Both inorganic organic NDVs studied agrochemical delivery in literature, but research on latter has slower develop than literature former. Since two classes exhibit significant differences surface chemistry, physical deformability, even colloidal stability, trends that apply may not hold NDVs, vice versa. We here review current uptake, translocation, biotransformation, cellular internalization plants following foliar root administration. A background plant physiology is provided as a leveling ground researchers field. Trends examined function NDV properties compared those reported nanomaterials. Methods assessing fate transport (a major bottleneck field) discussed. end by identifying knowledge gaps must be understood order rationally design precision nanodelivery.

Язык: Английский

Процитировано

10
Molecular dynamics in pharmaceutical nanotechnology: simulating interactions and advancing applications DOI
Anand B. Mundada, Prashanta Chandra Pradhan, Rajapandi Raju

и другие.

Journal of Biomaterials Science Polymer Edition, Год журнала: 2025, Номер unknown, С. 1 - 27

Опубликована: Янв. 9, 2025

Molecular Dynamics (MD) simulations are now widely utilized in pharmaceutical nanotechnology to gain deeper understanding of nanoscale processes imperative drug design. This review has also detailed how MD simulation can be employed the study drug-nanocarrier interactions, controlling release chemical compounds from delivery systems and increasing solubility bioavailability nanocarriers. Furthermore, contributes examining systems, measuring toxic effects, determining biocompatibility nanomedical systems. With incorporation artificial intelligence use hybrid gone a step ahead model other niches biology that make tremendous opening develop highly selective nanomedications. Nevertheless, with well-known issues such as computational constraints discrepancy between silico experiment results, remains work progress, considerable promise for replacing or supplementing existing approaches development precision medicine nanomedicine, continued progression healthcare hopeful.

Язык: Английский

Процитировано

1