Abstract
Cellular
biophysical
metrics
exhibit
systematic
alterations
during
processes,
such
as
metastasis
and
immune
cell
activation,
which
can
be
used
to
identify
separate
live
subpopulations
for
targeting
drug
screening.
Image‐based
cytometry
under
extensional
flows
accurately
quantify
deformability
based
on
shape
but
needs
extensive
image
reconstruction,
limits
its
inline
utilization
activate
sorting.
Impedance
measure
these
electric
field
screening,
while
frequency
response
offers
functional
information
viability
interior
structure,
are
difficult
discern
by
imaging.
Furthermore,
1‐D
temporal
impedance
signal
trains
characteristic
shapes
that
rapidly
templated
in
near
real‐time
extract
single‐cell
We
present
a
multilayer
perceptron
neural
network
templating
approach
utilizes
raw
signals
from
cells
flow,
alongside
training
with
corresponding
derive
net
electrical
anisotropy
over
wide
ranges
minimal
errors
size
distributions.
Deformability
physiology
applied
conjunction
the
same
multiparametric
classification
of
pancreatic
cancer
versus
associated
fibroblasts
using
support
vector
machine
model.
CNS Neuroscience & Therapeutics,
Год журнала:
2024,
Номер
30(10)
Опубликована: Окт. 1, 2024
Huntington's
disease
(HD)
is
a
devastating
neurodegenerative
that
manifested
by
gradual
loss
of
physical,
cognitive,
and
mental
abilities.
As
the
advances,
age
has
major
impact
on
pathogenic
signature
mutant
huntingtin
(mHTT)
protein
aggregation.
This
review
aims
to
explore
intricate
relationship
between
aging,
mHTT
toxicity,
cellular
senescence
in
HD.
Scientific
data
interplay
mHTT,
HD
were
collected
from
several
academic
databases,
including
PubMed,
Google
Scholar,
Google,
ScienceDirect.
The
search
terms
employed
"AGING,"
"HUNTINGTON'S
DISEASE,"
"MUTANT
HUNTINGTIN,"
"CELLULAR
SENESCENCE."
Additionally,
gather
information
molecular
mechanisms
potential
therapeutic
targets,
was
extended
include
relevant
such
as
"DNA
DAMAGE,"
"OXIDATIVE
STRESS,"
"AUTOPHAGY."
According
research,
aging
leads
worsening
pathophysiology
through
some
processes.
result
accumulation,
promoted,
which
causes
DNA
damage,
oxidative
stress,
decreased
autophagy,
increased
inflammatory
responses.
Pro-inflammatory
cytokines
other
substances
are
released
senescent
cells,
may
worsen
neuronal
damage
course
disease.
It
been
shown
treatments
directed
at
these
pathways
reduce
symptoms
enhance
longevity
experimental
animals,
pointing
new
possibility
treating
condition.
Through
their
amplification
harmful
effects
play
crucial
roles
development
Comprehending
interplays
creates
novel
opportunities
for
measures
targeted
alleviating
enhancing
patients'
quality
life.
Sensors and Actuators Reports,
Год журнала:
2024,
Номер
7, С. 100196 - 100196
Опубликована: Апрель 25, 2024
Fluorescence-activated
cell
sorting
(FACS)
holds
great
promise
for
the
separation
of
single
cells
or
populations
according
to
specific
light
scattering
and
fluorescent
characteristics.
Here,
we
present
a
new
perspective
on
microfluidic
FACS
(μFACS)
with
predictable
geometry,
which
meets
requirements
high-throughput
analysis
sorting.
Instead
widely
applied
elastic
polydimethylsiloxane
(PDMS),
rigid
epoxy
resin
chip
was
rapidly
fabricated
irreversibly
encapsulated
eliminate
channel
deformation
(tenfold
reduction)
enhance
performance
while
meeting
high
pressure
(>600
kPa)
flow
rate
application
scenarios.
Fluorescence
discrimination
particle
differentiation
were
additionally
validated
in
self-contained
μFACS
system
using
calibration
microspheres
mammalian
cells.
The
integrally
optimized
achieve
minimum
interval
(0.58
ms)
mean
1.5
m/s.
Ultimately,
event
recording
automated
accomplished
real
time
achieving
efficiency
87
%
at
throughput
8,000
events/s.
This
μFACS,
is
independent
physical
properties
could
pave
way
screening
plasma
samples
personalized
medicine.
Lab on a Chip,
Год журнала:
2024,
Номер
24(18), С. 4333 - 4343
Опубликована: Янв. 1, 2024
The
differences
in
the
cross-sectional
positions
of
cells
detection
area
have
a
severe
negative
impact
on
achieving
accurate
characterization
impedance
spectra
cells.
Herein,
we
proposed
three-dimensional
(3D)
inertial
focusing
based
cytometer
integrating
sheath
fluid
compression
and
for
high-accuracy
electrical
identification
tumor
First,
studied
effects
particle
initial
position
focusing.
Then,
relationship
height
signal-to-noise
ratio
(SNR)
signal
was
explored.
results
showed
that
efficient
single-line
7-20
μm
particles
close
to
electrodes
achieved
signals
with
high
SNR
low
coefficient
variation
(CV)
were
obtained.
Finally,
properties
three
types
(A549,
MDA-MB-231,
UM-UC-3
cells)
accurately
characterized.
Machine
learning
algorithms
implemented
identify
amplitude
phase
opacities
at
multiple
frequencies.
Compared
traditional
two-dimensional
(2D)
focusing,
accuracy
A549,
using
our
3D
increased
by
57.5%,
36.4%
36.6%,
respectively.
enables
wide
size
range
without
causing
clogging
obtains
signals,
improving
applicability
different
complex
biological
samples
cell
accuracy.
PLoS ONE,
Год журнала:
2025,
Номер
20(1), С. e0315435 - e0315435
Опубликована: Янв. 2, 2025
The
use
of
granulocyte
colony-stimulating
factor
(GCSF)
to
control
febrile
neutropenia
(FN)
caused
by
anti-cancer
chemotherapy
is
well
documented
but
it
still
needs
evaluated
with
respect
the
specific
type
cancer
and
chemotherapeutic
agents.
present
study
evaluates
efficacy
adjunctive
GCSF
for
treating
FN
after
taking
anticancer
therapy
measuring
clinical,
hematological
microbiological
outcomes.
It
a
single
center
conducted
at
Hayatabad
Medical
Complex
(HMC),
Peshawar,
Pakistan.
Adult
patients
both
genders,
suffering
from
different
types
sarcomas
were
included
in
study.
was
between
January
2023
2024.
Baseline
data
including
demographic
data,
medication
history
evaluation
all
recorded
time
enrolment.
Primary
outcomes
extent
absolute
neutrophil
count
(ANC)
recovery,
duration
severity
(grade
IV),
period
fever
resolution.
After
(with
without
GCSF)
clinical
outcomes,
compared
evaluated.
All
statistically
analyzed
SPSS
(IBMS,
version
20).
A
total
number
120
investigated
out
which
109
included.
Out
patients,
64
(58.72%)
received
therapy,
45
(41.28%)
did
not
receive
GCSF.
Comparison
showed
that
receiving
had
significant
improvement
ANC
recovery
time,
better
free
infections.
This
concluded
benefits
undergoing
treatment
carcinoma.
Mathematics,
Год журнала:
2025,
Номер
13(3), С. 433 - 433
Опубликована: Янв. 28, 2025
In
this
work,
it
is
taken
into
account
that
in
nature,
due
to
pressure
and
temperature,
water
drops
general
are
either
spherical
or
ellipsoidal.
Thus,
starting
from
a
more
structure,
3D
elliptical
surface
(oblate
spheroid)
constructed,
which,
by
means
of
parameters,
can
be
turned
shape.
Hexagons
built
on
rectangular
horizontal
plane,
then
plane
passed
through
an
at
height
h,
which
determined
parameter
θ.
As
result
the
cutting
these
surfaces,
curve
obtained,
both
ellipsoidal;
if
hexagons
within
perimeter
ellipse
obtained
as
function
θ,
they
marked
with
N,
outside
perimeter,
E.
Several
frozen
nucleation
centers
established,
same
layer
different
planes,
marking
them
F
their
first
eight
neighbors
B.
The
calculations
based
modified
snowflake
model
carried
out
tile
layer,
governed
thermodynamic
factors
α,
β,
γ,
leading
results
depend
position
nucleator,
symmetrical
asymmetrical
for
than
one
center
external
formed
vapor
functions
membrane.
