Electrophoresis,
Год журнала:
2023,
Номер
45(13-14), С. 1212 - 1232
Опубликована: Ноя. 1, 2023
Abstract
Single‐cell
biophysical
properties
play
a
crucial
role
in
regulating
cellular
physiological
states
and
functions,
demonstrating
significant
potential
the
fields
of
life
sciences
clinical
diagnostics.
Therefore,
over
last
few
decades,
researchers
have
developed
various
detection
tools
to
explore
relationship
between
changes
biological
cells
human
diseases.
With
rapid
advancement
modern
microfabrication
technology,
microfluidic
devices
quickly
emerged
as
promising
platform
for
single‐cell
analysis
offering
advantages
including
high‐throughput,
exceptional
precision,
ease
manipulation.
Consequently,
this
paper
provides
an
overview
recent
advances
systems
their
applications
field
cancer.
The
working
principles
latest
research
progress
property
are
first
analyzed,
highlighting
significance
electrical
mechanical
properties.
development
data
acquisition
processing
methods
real‐time,
practical
then
discussed.
Furthermore,
differences
tumor
normal
outlined,
illustrating
utilizing
cell
identification,
classification,
drug
response
assessment.
Lastly,
we
summarize
limitations
existing
properties,
while
also
pointing
out
prospects
future
directions
cancer
diagnosis
treatment.
ACS Sensors,
Год журнала:
2023,
Номер
8(3), С. 1183 - 1191
Опубликована: Март 3, 2023
Cellular
mechanical
property
analysis
reflecting
the
physiological
and
pathological
states
of
cells
plays
a
crucial
role
in
assessing
quality
stored
blood.
However,
its
complex
equipment
needs,
operation
difficulty,
clogging
issues
hinder
automated
rapid
biomechanical
testing.
Here,
we
propose
promising
biosensor
assisted
by
magnetically
actuated
hydrogel
stamping
to
fulfill
it.
The
flexible
magnetic
actuator
triggers
collective
deformation
multiple
light-cured
hydrogel,
it
allows
for
on-demand
bioforce
stimulation
with
advantages
portability,
cost-effectiveness,
simplicity
operation.
manipulated
cell
processes
are
captured
integrated
miniaturized
optical
imaging
system,
cellular
parameters
extracted
from
images
real-time
intelligent
sensing.
In
this
work,
30
clinical
blood
samples
different
storage
durations
(<14
days
>14
days)
were
tested.
A
deviation
3.3%
differentiation
system
compared
physician
annotation
demonstrated
feasibility.
This
should
broaden
application
assays
diverse
settings.
Microsystems & Nanoengineering,
Год журнала:
2024,
Номер
10(1)
Опубликована: Июнь 25, 2024
Abstract
During
cardiac
development,
mechanotransduction
from
the
in
vivo
microenvironment
modulates
cardiomyocyte
growth
terms
of
number,
area,
and
arrangement
heterogeneity.
However,
response
cells
to
different
degrees
mechanical
stimuli
is
unclear.
Organ-on-a-chip,
as
a
platform
for
investigating
stress
cellular
mimicry
microenvironment,
limited
by
lack
ability
accurately
quantify
externally
induced
stimuli.
previous
technology
lacks
integration
external
feedback
sensors
microfluidic
platforms
obtain
apply
precise
amounts
Here,
we
designed
cell
stretching
with
an
in-situ
sensor.
The
liquid
metal
can
measure
stimulation
caused
deformation
vacuum
cavity
exerted
on
cells.
was
applied
human
cardiomyocytes
(AC16)
under
cyclic
strain
(5%,
10%,
15%,
20
25%),
found
that
promoted
membrane
gradually
unify,
stabilized
at
15%
amplitude,
which
even
more
effective
after
3
days
culture.
platform’s
control
measurement
forces
be
used
establish
accurate
vitro
microenvironmental
models
disease
modeling
therapeutic
research.
Sensors,
Год журнала:
2024,
Номер
24(19), С. 6359 - 6359
Опубликована: Сен. 30, 2024
Cellular
heterogeneity
plays
a
significant
role
in
understanding
biological
processes,
such
as
cell
cycle
and
disease
progression.
Microfluidics
has
emerged
versatile
tool
for
manipulating
single
cells
analyzing
their
with
the
merits
of
precise
fluid
control,
small
sample
consumption,
easy
integration,
high
throughput.
Specifically,
integrating
microfluidics
electrical
techniques
provides
rapid,
label-free,
non-invasive
way
to
investigate
cellular
at
single-cell
level.
Here,
we
review
recent
development
microfluidic-based
strategies
manipulation
analysis,
including
dielectrophoresis-
electroporation-based
manipulation,
impedance-
AC
electrokinetic-based
methods,
electrochemical-based
detection
methods.
Finally,
challenges
future
perspectives
analysis
are
proposed.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 30, 2024
Imaging
flow
cytometry
allows
image-activated
cell
sorting
(IACS)
with
enhanced
feature
dimensions
in
cellular
morphology,
structure,
and
composition.
However,
existing
IACS
frameworks
suffer
from
the
challenges
of
3D
information
loss
processing
latency
dilemma
real-time
operation.
Herein,
we
establish
a
neuromorphic-enabled
video-activated
sorter
(NEVACS)
framework,
designed
to
achieve
high-dimensional
spatiotemporal
characterization
content
alongside
high-throughput
particles
wide
field
view.
NEVACS
adopts
event
camera,
CPU,
spiking
neural
networks
deployed
on
neuromorphic
chip,
achieves
throughput
1000
cells/s
relatively
economic
hybrid
hardware
solution
(~$10
K
for
control)
simple-to-make-and-use
microfluidic
infrastructures.
Particularly,
application
classifying
regular
red
blood
cells
blood-disease-relevant
spherocytes
highlights
accuracy
using
video
over
single
frame
(i.e.,
average
error
0.99%
vs
19.93%),
indicating
NEVACS'
potential
morphology
screening
disease
diagnosis.
The
use
of
the
intrinsic
electrical
properties
a
single
cell
by
broadband
impedance
spectroscopy
(EIS)
as
label-free
and
non-invasive
method
to
monitor
cellular
intracellular
features
is
an
emerging
field.
Here,
we
present
novel
EIS-based
sheathless
microfluidic
platform
with
integrated
coplanar
waveguide
probe
interior
cell.
This
allows
for
precise
single-cell
trapping
dielectrophoresis,
hydrodynamic
focusing,
sensing
trapped
We
measured
characteristics
Schizosaccharomyces
pombe
(fission)
yeast
frequency
sweep
(30
kHz
6GHz)
in
stagnant
sucrose
solution
using
two-port
scattering
(S)
parameters.
measurements
revealed
clear
distinction
between
cytoplasm
live
versus
dead
cells
at
3
GHz.
could
provide
real-time
monitoring
responses
chemical
physical
antagonists
diagnostic
purposes.
Nanotechnology and Precision Engineering,
Год журнала:
2025,
Номер
8(4)
Опубликована: Май 20, 2025
In
fluorescence
flow
cytometry,
spectral
overlap
among
multiple
fluorescent
labels
cannot
be
avoided,
and
thus
detected
intensities
need
to
compensated.
Although
compensation
in
cytometry
has
been
widely
used
for
many
years,
it
still
lacks
quantitative
evaluations
validate
its
effectiveness.
Using
a
home-developed
nine-color
cytometer,
this
study
first
obtains
calibration
curves
by
assaying
gradient
concentrations
of
nine
different
fluorochromes
individually,
with
the
highest
each
fluorochrome
being
obtain
spillover
matrix.
Mixed
solutions
are
analyzed
which
obtained
compensated
matrix,
translated
specific
based
on
curve
compared
nominal
values.
Three
mixed
Brilliant
Violet
650
711,
Alexa
Fluor
488
PE,
Pacific
Orange,
488,
PE
tested,
observed
reduce
excessive
signals
due
overlap.
Specifically,
concentration
deviations
(before
vs
after
compensation)
comparison
values
711
quantified
as
40.6%
14.9%
6.7%
1.9%,
respectively.
The
results
presented
here
provide
reference
that
can
effectively
address
issue
cytometry.
Biosensors,
Год журнала:
2022,
Номер
13(1), С. 13 - 13
Опубликована: Дек. 22, 2022
A
laboratory
blood
test
is
vital
for
assessing
a
patient’s
health
and
disease
status.
Advances
in
microfluidic
technology
have
opened
the
door
on-chip
analysis.
Currently,
devices
can
reproduce
myriad
routine
tests.
Considerable
progress
has
been
made
cytometry,
cell
separation,
characterization.
Along
with
usual
clinical
parameters,
microfluidics
makes
it
possible
to
determine
physical
properties
of
cells.
We
review
recent
advances
systems
measuring
biophysical
characteristics
Added
emphasis
placed
on
multifunctional
platforms
that
combine
several
technologies
effective
The
combination
hydrodynamic,
optical,
electromagnetic,
and/or
acoustic
methods
device
facilitates
precise
determination
various
analyzed
quantities
are
measured
by
parameters
determined
through
these
measurements.
discuss
unexplored
problems
present
our
perspectives
long-term
challenges
trends
associated
application
laboratories.
expect
characterization
cells
environment
be
considered
standard
future.
Lab on a Chip,
Год журнала:
2024,
Номер
24(16), С. 3930 - 3944
Опубликована: Янв. 1, 2024
A
novel
microfluidic
device
dedicated
to
cell
rheology
after
flow-induced
deformation
in
constricted
channel:
a
focus
on
the
recovery
properties
of
circulating
tumor
cells.
