Microfluidic characterization of single‐cell biophysical properties and the applications in cancer diagnosis DOI
Shanshan Li, Chun‐Dong Xue, Yong‐Jiang Li

и другие.

Electrophoresis, Год журнала: 2023, Номер 45(13-14), С. 1212 - 1232

Опубликована: Ноя. 1, 2023

Abstract Single‐cell biophysical properties play a crucial role in regulating cellular physiological states and functions, demonstrating significant potential the fields of life sciences clinical diagnostics. Therefore, over last few decades, researchers have developed various detection tools to explore relationship between changes biological cells human diseases. With rapid advancement modern microfabrication technology, microfluidic devices quickly emerged as promising platform for single‐cell analysis offering advantages including high‐throughput, exceptional precision, ease manipulation. Consequently, this paper provides an overview recent advances systems their applications field cancer. The working principles latest research progress property are first analyzed, highlighting significance electrical mechanical properties. development data acquisition processing methods real‐time, practical then discussed. Furthermore, differences tumor normal outlined, illustrating utilizing cell identification, classification, drug response assessment. Lastly, we summarize limitations existing properties, while also pointing out prospects future directions cancer diagnosis treatment.

Язык: Английский

Magnetically Actuated Hydrogel Stamping-Assisted Cellular Mechanical Analyzer for Stored Blood Quality Detection DOI
Le Yu, Longfei Chen, Yantong Liu

и другие.

ACS Sensors, Год журнала: 2023, Номер 8(3), С. 1183 - 1191

Опубликована: Март 3, 2023

Cellular mechanical property analysis reflecting the physiological and pathological states of cells plays a crucial role in assessing quality stored blood. However, its complex equipment needs, operation difficulty, clogging issues hinder automated rapid biomechanical testing. Here, we propose promising biosensor assisted by magnetically actuated hydrogel stamping to fulfill it. The flexible magnetic actuator triggers collective deformation multiple light-cured hydrogel, it allows for on-demand bioforce stimulation with advantages portability, cost-effectiveness, simplicity operation. manipulated cell processes are captured integrated miniaturized optical imaging system, cellular parameters extracted from images real-time intelligent sensing. In this work, 30 clinical blood samples different storage durations (<14 days >14 days) were tested. A deviation 3.3% differentiation system compared physician annotation demonstrated feasibility. This should broaden application assays diverse settings.

Язык: Английский

Процитировано

9

A review on intelligent impedance cytometry systems: Development, applications and advances DOI Creative Commons
Tao Tang, Trisna Julian,

Doudou Ma

и другие.

Analytica Chimica Acta, Год журнала: 2023, Номер 1269, С. 341424 - 341424

Опубликована: Май 29, 2023

Язык: Английский

Процитировано

9

Strain sensor on a chip for quantifying the magnitudes of tensile stress on cells DOI Creative Commons
Yuyin Zhang,

Yue Wang,

Hongze Yin

и другие.

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Июнь 25, 2024

Abstract During cardiac development, mechanotransduction from the in vivo microenvironment modulates cardiomyocyte growth terms of number, area, and arrangement heterogeneity. However, response cells to different degrees mechanical stimuli is unclear. Organ-on-a-chip, as a platform for investigating stress cellular mimicry microenvironment, limited by lack ability accurately quantify externally induced stimuli. previous technology lacks integration external feedback sensors microfluidic platforms obtain apply precise amounts Here, we designed cell stretching with an in-situ sensor. The liquid metal can measure stimulation caused deformation vacuum cavity exerted on cells. was applied human cardiomyocytes (AC16) under cyclic strain (5%, 10%, 15%, 20 25%), found that promoted membrane gradually unify, stabilized at 15% amplitude, which even more effective after 3 days culture. platform’s control measurement forces be used establish accurate vitro microenvironmental models disease modeling therapeutic research.

Язык: Английский

Процитировано

3

Microfluidic-Based Electrical Operation and Measurement Methods in Single-Cell Analysis DOI Creative Commons
Xing Liu, Xiaolin Zheng

Sensors, Год журнала: 2024, Номер 24(19), С. 6359 - 6359

Опубликована: Сен. 30, 2024

Cellular heterogeneity plays a significant role in understanding biological processes, such as cell cycle and disease progression. Microfluidics has emerged versatile tool for manipulating single cells analyzing their with the merits of precise fluid control, small sample consumption, easy integration, high throughput. Specifically, integrating microfluidics electrical techniques provides rapid, label-free, non-invasive way to investigate cellular at single-cell level. Here, we review recent development microfluidic-based strategies manipulation analysis, including dielectrophoresis- electroporation-based manipulation, impedance- AC electrokinetic-based methods, electrochemical-based detection methods. Finally, challenges future perspectives analysis are proposed.

Язык: Английский

Процитировано

3

Neuromorphic-enabled video-activated cell sorting DOI Creative Commons
Weihua He,

Junwen Zhu,

Yongxiang Feng

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 30, 2024

Imaging flow cytometry allows image-activated cell sorting (IACS) with enhanced feature dimensions in cellular morphology, structure, and composition. However, existing IACS frameworks suffer from the challenges of 3D information loss processing latency dilemma real-time operation. Herein, we establish a neuromorphic-enabled video-activated sorter (NEVACS) framework, designed to achieve high-dimensional spatiotemporal characterization content alongside high-throughput particles wide field view. NEVACS adopts event camera, CPU, spiking neural networks deployed on neuromorphic chip, achieves throughput 1000 cells/s relatively economic hybrid hardware solution (~$10 K for control) simple-to-make-and-use microfluidic infrastructures. Particularly, application classifying regular red blood cells blood-disease-relevant spherocytes highlights accuracy using video over single frame (i.e., average error 0.99% vs 19.93%), indicating NEVACS' potential morphology screening disease diagnosis.

Язык: Английский

Процитировано

3

High throughput cell stiffness measurement via multiplexed impedance sensors DOI
Norh Asmare,

A K M Arifuzzman,

Ningquan Wang

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117158 - 117158

Опубликована: Янв. 22, 2025

Язык: Английский

Процитировано

0

Label-Free Differentiation of Living versus Dead Single Yeast Cells using Broadband Electrical Impedance Spectroscopy DOI Creative Commons
Amirhossein Favakeh,

Amir Mokhtare,

Mohammad Javad Asadi

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The use of the intrinsic electrical properties a single cell by broadband impedance spectroscopy (EIS) as label-free and non-invasive method to monitor cellular intracellular features is an emerging field. Here, we present novel EIS-based sheathless microfluidic platform with integrated coplanar waveguide probe interior cell. This allows for precise single-cell trapping dielectrophoresis, hydrodynamic focusing, sensing trapped We measured characteristics Schizosaccharomyces pombe (fission) yeast frequency sweep (30 kHz 6GHz) in stagnant sucrose solution using two-port scattering (S) parameters. measurements revealed clear distinction between cytoplasm live versus dead cells at 3 GHz. could provide real-time monitoring responses chemical physical antagonists diagnostic purposes.

Язык: Английский

Процитировано

0

Effectiveness evaluation of fluorescent compensation in multicolor flow cytometry: A quantitative study DOI Creative Commons

Long Fan,

Chiyuan Gao,

Junbo Wang

и другие.

Nanotechnology and Precision Engineering, Год журнала: 2025, Номер 8(4)

Опубликована: Май 20, 2025

In fluorescence flow cytometry, spectral overlap among multiple fluorescent labels cannot be avoided, and thus detected intensities need to compensated. Although compensation in cytometry has been widely used for many years, it still lacks quantitative evaluations validate its effectiveness. Using a home-developed nine-color cytometer, this study first obtains calibration curves by assaying gradient concentrations of nine different fluorochromes individually, with the highest each fluorochrome being obtain spillover matrix. Mixed solutions are analyzed which obtained compensated matrix, translated specific based on curve compared nominal values. Three mixed Brilliant Violet 650 711, Alexa Fluor 488 PE, Pacific Orange, 488, PE tested, observed reduce excessive signals due overlap. Specifically, concentration deviations (before vs after compensation) comparison values 711 quantified as 40.6% 14.9% 6.7% 1.9%, respectively. The results presented here provide reference that can effectively address issue cytometry.

Язык: Английский

Процитировано

0

Microfluidic Systems for Blood and Blood Cell Characterization DOI Creative Commons
Hojin Kim, A. I. Zhbanov, Sung Yang

и другие.

Biosensors, Год журнала: 2022, Номер 13(1), С. 13 - 13

Опубликована: Дек. 22, 2022

A laboratory blood test is vital for assessing a patient’s health and disease status. Advances in microfluidic technology have opened the door on-chip analysis. Currently, devices can reproduce myriad routine tests. Considerable progress has been made cytometry, cell separation, characterization. Along with usual clinical parameters, microfluidics makes it possible to determine physical properties of cells. We review recent advances systems measuring biophysical characteristics Added emphasis placed on multifunctional platforms that combine several technologies effective The combination hydrodynamic, optical, electromagnetic, and/or acoustic methods device facilitates precise determination various analyzed quantities are measured by parameters determined through these measurements. discuss unexplored problems present our perspectives long-term challenges trends associated application laboratories. expect characterization cells environment be considered standard future.

Язык: Английский

Процитировано

12

Deformation under flow and morphological recovery of cancer cells DOI Creative Commons

Emile Gasser,

Émilie Su, Kotryna Vaidžiulytė

и другие.

Lab on a Chip, Год журнала: 2024, Номер 24(16), С. 3930 - 3944

Опубликована: Янв. 1, 2024

A novel microfluidic device dedicated to cell rheology after flow-induced deformation in constricted channel: a focus on the recovery properties of circulating tumor cells.

Язык: Английский

Процитировано

2