Engineering Peptide-Based Molecular Baits for Targeted Fishing and Protein Profiling of Exosomes as a Liquid Biopsy for Gastrointestinal Adenocarcinoma DOI
Kun Xu, Yanting Hao, Han Gao

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 741 - 748

Опубликована: Дек. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Язык: Английский

Magnetic Polysaccharide Mesenchymal Stem Cells Exosomes Delivery Microcarriers for Synergistic Therapy of Osteoarthritis DOI
Lei Yang, Wenzhao Li, Yuanjin Zhao

и другие.

ACS Nano, Год журнала: 2024, Номер 18(31), С. 20101 - 20110

Опубликована: Июль 23, 2024

Osteoarthritis (OA) is a prevalent degenerative disease that afflicts more than 250 million people worldwide, impairing their mobility and quality of life. However, conventional drug therapy palliative. Exosomes (Exo), although with the potential to fundamentally repair cartilage, face challenges in efficient enrichment delivery. In this study, we developed magnetic polysaccharide hydrogel particles as microcarriers for synergistic OA. The were composed modified natural polysaccharides, hyaluronic acid (HAMA), chondroitin sulfate (CSMA), generated from microfluidic electrospray combination cryogelation process. Magnetic nanoparticles spiny structures capable capturing stem cell Exo encapsulated within together an anti-inflammatory diclofenac sodium (DS). released DS had effect alleviating OA symptoms promoting cartilage repair. vitro vivo results demonstrated excellent performance microcarrier treatment. We believe work has other related diseases.

Язык: Английский

Процитировано

19
Cationic-motif-modified exosomes for mRNA delivery to retinal photoreceptors DOI Creative Commons
Héctor A. Millán Cotto, Tanvi Vinod Pathrikar, Bill Hakim

и другие.

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(30), С. 7384 - 7400

Опубликована: Янв. 1, 2024

Topical treatment of vitreoretinal diseases remains a challenge due to slow corneal uptake and systemic clearance. Exosomes are emerging nanocarriers for drug delivery biocompatibility cellular targeting properties. To apply them retinal

Язык: Английский

Процитировано

8
Cartilage-targeting Exosomes for Delivery of Receptor Antagonist of Interleukin-1 in Osteoarthritis treatment DOI Creative Commons
Tanvi Vinod Pathrikar,

Helna Mary Baby,

Bill Hakim

и другие.

Osteoarthritis and Cartilage, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

1
Current status, opportunities and challenges of exosomes in diagnosis and treatment of osteoarthritis DOI
Hamza Malik Okuyan,

Ayça Coşkun,

Mehmet A. Begen

и другие.

Life Sciences, Год журнала: 2025, Номер 362, С. 123365 - 123365

Опубликована: Янв. 4, 2025

Язык: Английский

Процитировано

0
An anti-CD19-exosome delivery system navigates the blood–brain barrier for targeting of central nervous system lymphoma DOI Creative Commons
Meifang Zhao, Qi Li,

Yali Chai

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 5, 2025

High-dose methotrexate (HD-MTX) serves as the cornerstone of central nervous system lymphoma (CNSL) treatment, but its efficacy is limited due to low blood-brain barrier (BBB) penetration and adverse effects. This study focused on an exosome-based drug delivery approach aimed at enhancing BBB permeability, thereby reducing required dosage (MTX) while ensuring specific targeting CNSL. Human adipose-derived mesenchymal stem cells (hAMSCs) were modified with a lentiviral vector encoding anti-CD19, incorporated into exosomes characterized by colloidal gold immunoelectron microscopy Nano flow cytometry. MTX loaded anti-CD19-Exos via co-incubation, assessed for loading encapsulation efficiencies using HPLC. In vitro model constructed hCMEC/D3 astrocytes investigate permeability. vivo anti-CD19-Exo-MTX evaluated in intracranial CNSL models MRI. Biodistribution tracked DiR-labeled exosomes, concentration CSF measured LC-MS/MS identified exosomal proteins analyzed GO Analysis. Neuroprotective effects TUNEL Nissl staining hippocampal neurons models. Liver kidney pathology, blood biochemical markers, complete count protein organ protection MTX-induced myelosuppression. We generated anti-CD19-Exo derived from hAMSCs. These adapted effectively encapsulated MTX, accessibility within sustained intracellular accumulation over extended period. Notably, interacted cerebrovascular endothelial BBB, leading endocytosis facilitating transportation across barrier. Anti-CD19-Exo-MTX outperformed free vitro, exhibiting more potent lymphoma-suppressive effect (P < 0.05). orthotopic models, exhibited significantly reduced disease burden compared both Exo-MTX groups, along prolonged overall survival analysis demonstrated enhanced stability longer-lasting levels anti-CD19-Exo-MTX. precise no toxicity. our highlighted functional potential reversal hAMSCs-exosomes neurotoxicity, hepatic renal impairment, present promising platform that enhances permeability offers effective treatment

Язык: Английский

Процитировано

0
Cartilage targeting cationic peptide carriers display deep cartilage penetration and retention in a rabbit model of post-traumatic osteoarthritis DOI
Timothy L. Boyer,

Olivia Chao,

Bill Hakim

и другие.

Osteoarthritis and Cartilage, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Home delivery for chondrocytes DOI
Christopher H. Evans

Osteoarthritis and Cartilage, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Exosome nanovesicles: biomarkers and new strategies for treatment of human diseases DOI Creative Commons

Chuan Xu,

Chaoyang Jiang, Zhihui Li

и другие.

MedComm, Год журнала: 2024, Номер 5(8)

Опубликована: Июль 15, 2024

Exosomes are nanoscale vesicles of cellular origin. One the main characteristics exosomes is their ability to carry a wide range biomolecules from parental cells, which important mediators intercellular communication and play an role in physiological pathological processes. have advantages biocompatibility, low immunogenicity, biodistribution. As researchers' understanding has increased, various strategies been proposed for use diagnosing treating diseases. Here, we provide overview biogenesis composition exosomes, describe relationship between disease progression, focus on as biomarkers early screening, monitoring, guiding therapy refractory diseases such tumors neurodegenerative We also summarize current applications especially engineered efficient drug delivery, targeted therapies, gene immune vaccines. Finally, challenges potential research directions clinical application discussed. In conclusion, emerging molecule that can be used diagnosis treatment diseases, combined with multidisciplinary innovative solutions, will applications.

Язык: Английский

Процитировано

2
Charge-Reversal Nano-Drug Delivery Systems in the Tumor Microenvironment: Mechanisms, Challenges, and Therapeutic Applications DOI Open Access

Yizhu Liang,

Jiashuai Wu,

Yutong Yan

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9779 - 9779

Опубликована: Сен. 10, 2024

The charge-reversal nano-drug delivery system (CRNDDS) is a promising for delivering chemotherapy drugs and has gained widespread application in cancer treatment. In this review, we summarize the recent advancements CRNDDSs terms of We also delve into mechanism CRNDDSs, focusing on acid-responsive, redox-responsive, enzyme-responsive mechanisms. This study elucidates how these systems undergo charge transitions response to specific microenvironmental stimuli commonly found tumor tissues. Furthermore, review explores pivotal role diagnosis treatment, their potential limitations. By leveraging unique physiological characteristics tumors, such as acidic pH, redox potential, enzyme activity, demonstrate enhanced accumulation penetration at sites, resulting improved therapeutic efficacy diagnostic accuracy. implications highlight drug novel targeted strategy therapy diagnosis.

Язык: Английский

Процитировано

2
Engineering Peptide-Based Molecular Baits for Targeted Fishing and Protein Profiling of Exosomes as a Liquid Biopsy for Gastrointestinal Adenocarcinoma DOI
Kun Xu, Yanting Hao, Han Gao

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 741 - 748

Опубликована: Дек. 30, 2024

High-performance isolation of exosomes as a promising liquid biopsy target is great importance for both fundamental research and clinical applications. This is, however, challenged by the prevalent heterogeneity highly complex nature biosamples. Here, we introduce use CD81-targeting peptide building block tailoring molecular baits exosome payload analysis in biofluids. To explore full potential multivalent interactions, peptide-functionalized affinity interfaces were covalently engineered with varied assembling topology, flexibility, local density recognition motif. Capable best fitting surface conformation CD81 on curved membranes, dual-layered capture interface (Exo-PepTrap2) tandem bivalent decoration outperforms monolayered branched architectures. Enabled multivalency-enhanced reaction antifouling ability, Exo-PepTrap2 achieved high yield purity targeted fishing cell culture media urine samples. By integration mass spectrometry-based proteomic profiling, differentially expressed proteins efficiently identified harvested biomarkers gastrointestinal adenocarcinoma. CD81-targeted platform provides new viewpoint multivalency-based versatile tool to information precise medicine.

Язык: Английский

Процитировано

1