iScience,
Год журнала:
2023,
Номер
26(10), С. 107958 - 107958
Опубликована: Сен. 20, 2023
Alternative
splicing
(AS)
regulation
has
an
essential
role
in
complex
diseases.
However,
the
AS
profiles
hippocampal
(HIPPO)
region
of
human
brain
are
underexplored.
Here,
we
investigated
cis-acting
sQTLs
HIPPO
264
samples
and
identified
thousands
significant
sQTLs.
By
enrichment
analysis
functional
characterization
these
sQTLs,
found
that
were
enriched
among
histone-marked
regions,
transcription
factors
binding
sites,
RNA
proteins
disorders-associated
loci.
Comparative
analyses
with
dorsolateral
prefrontal
cortex
revealed
importance
(rg
=
0.87).
Furthermore,
performed
a
transcriptome-wide
association
study
Alzheimer's
disease
16
genes
whose
genetically
regulated
levels
may
have
causal
Alzheimer.
Overall,
our
improves
knowledge
transcriptome
gene
provides
novel
insights
into
elucidating
pathogenesis
potential
associated
disorders.
FEBS Letters,
Год журнала:
2023,
Номер
597(7), С. 947 - 961
Опубликована: Март 1, 2023
Cytoplasmic
capping
returns
a
cap
to
specific
mRNAs,
thus
protecting
uncapped
RNAs
from
decay.
Prior
the
identification
of
cytoplasmic
capping,
mRNAs
were
thought
be
degraded.
Here,
we
test
whether
long
noncoding
(lncRNAs)
are
substrates
enzyme
(cCE).
The
subcellular
localisation
14
lncRNAs
associated
with
sarcomas
examined
in
U2OS
osteosarcoma
cells.
We
used
5′
rapid
amplification
cDNA
ends
(RACE)
assay
forms
these
lncRNAs.
Inhibiting
elevated
selected
indicating
plausible
role
cCE
targeting
them.
Analysis
published
analysis
gene
expression
(CAGE)
data
shows
increased
prevalence
certain
5’‐RACE
cloned
sequences,
suggesting
that
targets
capping.
Frontiers in Oncology,
Год журнала:
2023,
Номер
13
Опубликована: Июнь 13, 2023
Esophageal
squamous
cell
carcinoma
(ESCC)
is
the
most
prevalent
histological
esophageal
cancer
characterized
by
advanced
diagnosis,
metastasis,
resistance
to
treatment,
and
frequent
recurrence.
In
recent
years,
numerous
human
disorders
such
as
ESCC,
have
been
linked
abnormal
expression
of
circular
RNAs
(circRNAs),
suggesting
that
they
are
fundamental
intricate
system
gene
regulation
governs
ESCC
formation.
The
tumor
microenvironment
(TME),
referring
area
surrounding
cells,
composed
multiple
components,
including
stromal
immune
vascular
system,
extracellular
matrix
(ECM),
signaling
molecules.
this
review,
we
briefly
described
biological
purposes
mechanisms
aberrant
circRNA
in
TME
microenvironment,
angiogenesis,
epithelial-to-mesenchymal
transition,
hypoxia,
metabolism,
radiotherapy
resistance.
As
in-depth
research
into
processes
circRNAs
continues,
promising
therapeutic
targets
or
delivery
systems
for
therapy
diagnostic
prognostic
indicators
ESCC.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 6, 2024
Abstract
Hypoxia-inducible
factors
(HIFs)
regulate
the
expression
of
several
hypoxia-responsive
RNAs
(HRR)
to
sustain
cellular
homeostasis.
Cytoplasmic
pool
mRNA
capping
enzyme
(cCE)
has
been
shown
recap
its
substrate
uncapped
mRNAs
or
long
noncoding
(lncRNAs)
present
in
cytoplasm,
preventing
their
degradation,
even
during
arsenite-induced
oxidative
stress.
Here,
we
examined
effect
hypoxia
on
cCE
and
function
regulating
lncRNAs.
Hypoxia
induces
HIF1α
dependent
manner
as
evidenced
by
Chromatin
immunoprecipitation
inhibitor
experiments.
Furthermore,
found
post-transcriptionally
controls
stability
target
lncRNAs
amidst
hypoxia.
These
results
suggest
that
may
act
a
posttranscriptional
modulator
could
alter
response
stabilizing
cCE-targeted
Experimental Eye Research,
Год журнала:
2024,
Номер
unknown, С. 110190 - 110190
Опубликована: Дек. 1, 2024
Restricted
oxygen
supply
in
the
aging
eye
may
lead
to
hypoxic
conditions
outer
retina
and
contribute
not
only
physiological
but
also
nonhereditary
degenerative
retinal
diseases.
To
understand
response
of
specific
cell
types,
we
performed
single-cell
RNA
sequencing
retinas
isolated
from
mice
exposed
hypoxia.
Significantly
upregulated
expression
marker
genes
clusters
confirmed
a
general
transcriptional
By
focusing
on
photoreceptors,
identified
kinesin
motor
protein
(Kif4)
that
was
specifically
strongly
induced
cones.
In
contrast,
RNA-binding
proteins
Rbm3
Cirbp
were
differentially
expressed
across
demonstrated
isoform
switching
The
resulting
short
variants
these
gene
transcripts
are
connected
epitranscriptomic
regulation,
notion
supported
by
differential
writers,
readers
erasers
m
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Июль 17, 2022
Abstract
Cytoplasmic
capping
returns
a
cap
to
specific
mRNAs
thus
protecting
uncapped
RNAs
from
decay.
Prior
the
identification
of
cytoplasmic
capping,
were
thought
be
degraded.
Here,
we
test
whether
long
non-coding
(lncRNAs)
are
enzyme
(cCE)
substrates.
The
subcellular
localization
fourteen
lncRNAs
associated
with
sarcomas
examined
in
U2OS
osteosarcoma
cells.
We
used
5’
rapid
amplification
cDNA
ends
(RACE)
assay
forms
these
lncRNAs.
Inhibiting
elevated
selected
indicating
plausible
role
cCE
targeting
them.
Analysis
published
analysis
gene
expression
(CAGE)
data
shows
increased
prevalence
certain
5’-RACE
cloned
sequences
suggesting
that
targets.
