Identification of region-specific splicing QTLs in human hippocampal tissue and its distinctive role in brain disorders DOI Creative Commons
Xiaoyan Li, Yiran Zhao, Hui Kong

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107958 - 107958

Published: Sept. 20, 2023

Alternative splicing (AS) regulation has an essential role in complex diseases. However, the AS profiles hippocampal (HIPPO) region of human brain are underexplored. Here, we investigated cis-acting sQTLs HIPPO 264 samples and identified thousands significant sQTLs. By enrichment analysis functional characterization these sQTLs, found that were enriched among histone-marked regions, transcription factors binding sites, RNA proteins disorders-associated loci. Comparative analyses with dorsolateral prefrontal cortex revealed importance (rg = 0.87). Furthermore, performed a transcriptome-wide association study Alzheimer's disease 16 genes whose genetically regulated levels may have causal Alzheimer. Overall, our improves knowledge transcriptome gene provides novel insights into elucidating pathogenesis potential associated disorders.

Language: Английский

Exploring Epigenetic and Genetic Modulation in Animal Responses to Thermal Stress DOI
Jianfang Wang, Sayed Haidar Abbas Raza, Sameer D. Pant

et al.

Molecular Biotechnology, Journal Year: 2024, Volume and Issue: 67(3), P. 942 - 956

Published: March 25, 2024

Language: Английский

Citations

5
Hippocampal microRNA-181a overexpression participates in anxiety and ethanol related behaviors via regulating the expression of SIRT-1 DOI
Amine Bahí

Physiology & Behavior, Journal Year: 2025, Volume and Issue: unknown, P. 114839 - 114839

Published: Feb. 1, 2025

Language: Английский

Citations

0
Pharmacology of Epitranscriptomic Modifications: Decoding the Therapeutic Potential of RNA Modifications in Drug Resistance DOI
Abdullah Alkhammash

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: 994, P. 177397 - 177397

Published: Feb. 18, 2025

Language: Английский

Citations

0
Long noncoding RNAs are substrates for cytoplasmic capping enzyme DOI
Avik Mukherjee, Safirul Islam, Rachel E. Kieser

et al.

FEBS Letters, Journal Year: 2023, Volume and Issue: 597(7), P. 947 - 961

Published: March 1, 2023

Cytoplasmic capping returns a cap to specific mRNAs, thus protecting uncapped RNAs from decay. Prior the identification of cytoplasmic capping, mRNAs were thought be degraded. Here, we test whether long noncoding (lncRNAs) are substrates enzyme (cCE). The subcellular localisation 14 lncRNAs associated with sarcomas examined in U2OS osteosarcoma cells. We used 5′ rapid amplification cDNA ends (RACE) assay forms these lncRNAs. Inhibiting elevated selected indicating plausible role cCE targeting them. Analysis published analysis gene expression (CAGE) data shows increased prevalence certain 5’‐RACE cloned sequences, suggesting that targets capping.

Language: Английский

Citations

6
Roles of circRNA dysregulation in esophageal squamous cell carcinoma tumor microenvironment DOI Creative Commons

Jingyi Li,

Song Yu-xia,

Huihong Cai

et al.

Frontiers in Oncology, Journal Year: 2023, Volume and Issue: 13

Published: June 13, 2023

Esophageal squamous cell carcinoma (ESCC) is the most prevalent histological esophageal cancer characterized by advanced diagnosis, metastasis, resistance to treatment, and frequent recurrence. In recent years, numerous human disorders such as ESCC, have been linked abnormal expression of circular RNAs (circRNAs), suggesting that they are fundamental intricate system gene regulation governs ESCC formation. The tumor microenvironment (TME), referring area surrounding cells, composed multiple components, including stromal immune vascular system, extracellular matrix (ECM), signaling molecules. this review, we briefly described biological purposes mechanisms aberrant circRNA in TME microenvironment, angiogenesis, epithelial-to-mesenchymal transition, hypoxia, metabolism, radiotherapy resistance. As in-depth research into processes circRNAs continues, promising therapeutic targets or delivery systems for therapy diagnostic prognostic indicators ESCC.

Language: Английский

Citations

4
LncRNA MALAT 1/miR-625–3p/HIF-1α axis regulates the EMT of hypoxia-induced RPE cells by activating NF-κB/snail signaling DOI
Lirong Zou, Xinling Wang, Xiao Han

et al.

Experimental Cell Research, Journal Year: 2023, Volume and Issue: 429(1), P. 113650 - 113650

Published: May 18, 2023

Language: Английский

Citations

3
Cytoplasmic mRNA capping enzyme is regulated by hypoxia inducible factor HIF1α and controls stability of the target lncRNAs during hypoxia DOI
Safirul Islam, Chandrama Mukherjee

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 6, 2024

Abstract Hypoxia-inducible factors (HIFs) regulate the expression of several hypoxia-responsive RNAs (HRR) to sustain cellular homeostasis. Cytoplasmic pool mRNA capping enzyme (cCE) has been shown recap its substrate uncapped mRNAs or long noncoding (lncRNAs) present in cytoplasm, preventing their degradation, even during arsenite-induced oxidative stress. Here, we examined effect hypoxia on cCE and function regulating lncRNAs. Hypoxia induces HIF1α dependent manner as evidenced by Chromatin immunoprecipitation inhibitor experiments. Furthermore, found post-transcriptionally controls stability target lncRNAs amidst hypoxia. These results suggest that may act a posttranscriptional modulator could alter response stabilizing cCE-targeted

Language: Английский

Citations

0
LncRNA H19 improves mesenchymal characteristics of buffalo (Bubalus bubalis) bone marrow-derived mesenchymal stem cells under hypoxic conditions DOI
Lulu Wang, Jun Zhang, Sheng Ye

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 182, P. 105461 - 105461

Published: Nov. 22, 2024

Language: Английский

Citations

0
Oxygen-dependent alternative mRNA splicing and a cone-specific motor protein revealed by single-cell RNA sequencing in hypoxic retinas DOI Creative Commons
Lynn J. A. Ebner, Duygu Karademir,

Sarah Nötzli

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: unknown, P. 110190 - 110190

Published: Dec. 1, 2024

Restricted oxygen supply in the aging eye may lead to hypoxic conditions outer retina and contribute not only physiological but also nonhereditary degenerative retinal diseases. To understand response of specific cell types, we performed single-cell RNA sequencing retinas isolated from mice exposed hypoxia. Significantly upregulated expression marker genes clusters confirmed a general transcriptional By focusing on photoreceptors, identified kinesin motor protein (Kif4) that was specifically strongly induced cones. In contrast, RNA-binding proteins Rbm3 Cirbp were differentially expressed across demonstrated isoform switching The resulting short variants these gene transcripts are connected epitranscriptomic regulation, notion supported by differential writers, readers erasers m

Language: Английский

Citations

0
Identification of long non-coding RNAs as substrates for cytoplasmic capping enzyme DOI Open Access
Avik Mukherjee, Safirul Islam, Rachel E. Kieser

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: July 17, 2022

Abstract Cytoplasmic capping returns a cap to specific mRNAs thus protecting uncapped RNAs from decay. Prior the identification of cytoplasmic capping, were thought be degraded. Here, we test whether long non-coding (lncRNAs) are enzyme (cCE) substrates. The subcellular localization fourteen lncRNAs associated with sarcomas examined in U2OS osteosarcoma cells. We used 5’ rapid amplification cDNA ends (RACE) assay forms these lncRNAs. Inhibiting elevated selected indicating plausible role cCE targeting them. Analysis published analysis gene expression (CAGE) data shows increased prevalence certain 5’-RACE cloned sequences suggesting that targets.

Language: Английский

Citations

1