Biology, Год журнала: 2024, Номер 13(6), С. 391 - 391
Опубликована: Май 29, 2024
Methyltransferases are a wide-ranging, yet well-conserved, class of molecules that have been found to modify wide variety substrates. Interest in RNA methylation has surged recent years with the identification major eukaryotic mRNA m6A methyltransferase METTL3. METTL16 also identified as an methyltransferase; however, much less is known about its targets and actions. Interestingly, addition their catalytic activities, both METTL3 “methylation-independent” functions, including translational regulation, which discovered. However, evidence suggests METTL16’s role RNA-binding protein may be more significant than currently recognized. In this review, we will introduce methylation, specifically m6A, enzymes responsible for deposition. We discuss varying roles these perform delve deeper into possible methylation-independent binding proteins. Finally, touch upon many open questions still remaining.
Язык: Английский
Процитировано
5Unraveling the epigenetic landscape: m6A modifications in spermatogenesis and their implications for azoospermia
Deleted Journal, Год журнала: 2025, Номер 2(1)
Опубликована: Фев. 3, 2025
N6-methyladenosine (m6A) RNA modifications have emerged as crucial epigenetic regulators in various biological processes, including spermatogenesis. This review aims to elucidate the role of m6A spermatogenesis and their implications for male infertility, particularly non-obstructive azoospermia (NOA). We conducted a comprehensive recent studies focusing on methyltransferases, demethylases, reader proteins involved Our analysis reveals that play vital regulating mRNA stability, translation efficiency, splicing, RNA–protein interactions during Key such METTL3, METTL14, ALKBH5, YTHDF are critical proper spermatogenic function. Abnormalities these regulators, due genetic mutations, expression dysregulation, or environmental factors, linked impaired NOA. Studies also indicate significant dysregulation m6A-related genes NOA patients, suggesting potential diagnostic therapeutic target infertility. integral regulation fertility. Further research is needed fully understand mechanisms by which influence explore interventions. highlights importance reproductive health underscores need continued investigation this emerging field.
Язык: Английский
Процитировано
0Pharmacology of Epitranscriptomic Modifications: Decoding the Therapeutic Potential of RNA Modifications in Drug Resistance
European Journal of Pharmacology, Год журнала: 2025, Номер 994, С. 177397 - 177397
Опубликована: Фев. 18, 2025
Язык: Английский
Процитировано
0Comprehensive nucleoside analysis of archaeal RNA modification profiles reveals an m7G in the conserved P loop of 23S rRNA
Cell Reports, Год журнала: 2025, Номер 44(4), С. 115471 - 115471
Опубликована: Март 25, 2025
Язык: Английский
Процитировано
0Sci-ModoM: a quantitative database of transcriptome-wide high-throughput RNA modification sites
Nucleic Acids Research, Год журнала: 2024, Номер 53(D1), С. D310 - D317
Опубликована: Ноя. 5, 2024
We present Sci-ModoM, the first next-generation RNome database offering a holistic view of epitranscriptomic landscape. Sci-ModoM has simple yet powerful interface, underpinned by FAIR data principles, standardized nomenclature, and interoperable formats, fostering use common standards within epitranscriptomics community. provides quantitative measurements per site dataset, enabling users to assess confidence levels based on score, coverage, stoichiometry. Data in is directly traceable its sources. Users can Search Compare over six million modifications across 156 datasets, Browse or download retrieve metadata. A comparison tool offers novel unique opportunity compare site-wise with ability securely upload user against latest published research. empowers researchers, including non-experts, access broad spectrum recent RNA modification data, thereby enhancing utility impact discoveries, opening new avenues biological medical
Язык: Английский
Процитировано
2The role of lncRNA binding to RNA‑binding proteins to regulate mRNA stability in cancer progression and drug resistance mechanisms (Review)
Oncology Reports, Год журнала: 2024, Номер 52(5)
Опубликована: Авг. 23, 2024
Язык: Английский
Процитировано
1Rationalizing the Effects of RNA Modifications on Protein Interactions
Molecular Therapy — Nucleic Acids, Год журнала: 2024, Номер 35(4), С. 102391 - 102391
Опубликована: Ноя. 15, 2024
RNA modifications play a crucial role in regulating gene expression by altering structure and modulating interactions with RNA-binding proteins (RBPs). In this study, we explore the impact of specific chemical modifications-N
Язык: Английский
Процитировано
1Rationalizing the Effects of RNA Modifications on Protein Interactions
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 31, 2024
Abstract RNA modifications play a crucial role in regulating gene expression by altering structure and modulating interactions with RNA-binding proteins (RBPs). In this study, we explore the impact of specific chemical modifications—N6-methyladenosine (m⁶A), A-to-I editing, pseudouridine (Ψ)—on secondary protein-RNA interactions. Utilizing genome-wide data, including predictions interaction datasets, classify into distinct categories based on their binding behaviors: modification-specific structure-independent, or modification-promiscuous structure-dependent. For instance, m⁶A readers like YTHDF2 exhibit structure-independent binding, consistently attaching to regardless structural changes. Conversely, such as U2AF2 display structure-dependent behavior, preferences response changes induced different modifications. which causes significant changes, typically reduces protein interactions, while Ψ enhances stability, albeit variable effects binding. To better predict these developed catRAPID 2.0 algorithm, forecasts propensities. This algorithm serves valuable tool for researchers, enabling prediction analysis modifications’ thus offering new insights biology engineering. The is available at http://service.tartaglialab.com/new_submission/catrapid_omicsv2_rna_mod . Figure
Язык: Английский
Процитировано
0RNA修饰的检测和定点干预
Scientia Sinica Chimica, Год журнала: 2024, Номер unknown
Опубликована: Дек. 1, 2024
Процитировано
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