Plants,
Год журнала:
2021,
Номер
10(11), С. 2409 - 2409
Опубликована: Ноя. 8, 2021
Metabolomics
is
now
considered
a
wide-ranging,
sensitive
and
practical
approach
to
acquire
useful
information
on
the
composition
of
metabolite
pool
present
in
any
organism,
including
plants.
Investigating
metabolomic
regulation
plants
essential
understand
their
adaptation,
acclimation
defense
responses
environmental
stresses
through
production
numerous
metabolites.
Moreover,
metabolomics
can
be
easily
applied
for
phenotyping
plants;
thus,
it
has
great
potential
used
genome
editing
programs
develop
superior
next-generation
crops.
This
review
describes
recent
analytical
tools
techniques
available
study
metabolome,
along
with
significance
sample
preparation
using
targeted
non-targeted
methods.
Advanced
tools,
like
gas
chromatography-mass
spectrometry
(GC-MS),
liquid
chromatography
mass-spectroscopy
(LC-MS),
capillary
electrophoresis-mass
(CE-MS),
fourier
transform
ion
cyclotron
resonance-mass
(FTICR-MS)
matrix-assisted
laser
desorption/ionization
(MALDI),
mobility
(IMS)
nuclear
magnetic
resonance
(NMR)
have
speed
up
precise
metabolic
profiling
Further,
we
provide
complete
overview
bioinformatics
plant
metabolome
database
that
utilized
advance
our
knowledge
biology.
1H
NMR
spectra
from
urine
can
yield
information-rich
data
sets
that
offer
important
insights
into
many
biological
and
biochemical
phenomena.
However,
the
quality
utility
of
these
be
profoundly
affected
by
how
are
processed
interpreted.
For
instance,
if
incorrectly
referenced
or
inconsistently
aligned,
identification
compounds
will
incorrect.
If
mis-phased
baseline
correction
is
flawed,
estimated
concentrations
systematically
biased.
Furthermore,
because
permits
measurement
spanning
up
to
five
orders
magnitude,
several
problems
arise
with
analysis.
signals
originating
most
abundant
metabolites
may
prove
least
biologically
relevant
while
arising
but
hardest
accurately
precisely
measure.
As
a
result,
number
processing
techniques
such
as
scaling,
transformation
normalization
often
required
address
issues.
Therefore,
proper
critical
step
correctly
extract
useful
information
in
any
NMR-based
metabolomic
study.
In
this
review
we
highlight
significance,
advantages
disadvantages
different
spectral
steps
common
studies
urine.
These
include:
chemical
shift
referencing,
phase
correction,
alignment,
binning,
scaling
normalization.
We
also
provide
set
recommendations
for
best
practices
regarding
biofluids,
particular
focus
on
Metabolites,
Год журнала:
2020,
Номер
10(2), С. 47 - 47
Опубликована: Янв. 26, 2020
Metabolomic
profiling
of
biofluids,
e.g.,
urine,
plasma,
has
generated
vast
and
ever-increasing
amounts
knowledge
over
the
last
few
decades.
Paradoxically,
metabolomic
analysis
saliva,
most
readily-available
human
biofluid,
lagged.
This
review
explores
history
saliva-based
metabolomics
summarizes
current
salivary
metabolomics.
Current
applications
have
largely
focused
on
diagnostic
biomarker
discovery
value
literature
base
is
explored.
There
also
a
small,
albeit
promising,
concerning
use
in
monitoring
athletic
performance.
Functional
roles
metabolites
remain
unexplored.
Areas
emerging
include
role
oral
host–microbiome
interactions
shaping
metabolite
profile
potential
physiology,
taste
perception.
Discussion
future
research
directions
describes
need
to
begin
acquiring
greater
function
metabolites,
direction
field
gut
metabolome.
The
saliva
as
an
easily
obtainable,
information-rich
fluid
that
could
complement
other
gastrointestinal
fluids
exploration
metabolome
emphasized.
Plants,
Год журнала:
2021,
Номер
10(11), С. 2409 - 2409
Опубликована: Ноя. 8, 2021
Metabolomics
is
now
considered
a
wide-ranging,
sensitive
and
practical
approach
to
acquire
useful
information
on
the
composition
of
metabolite
pool
present
in
any
organism,
including
plants.
Investigating
metabolomic
regulation
plants
essential
understand
their
adaptation,
acclimation
defense
responses
environmental
stresses
through
production
numerous
metabolites.
Moreover,
metabolomics
can
be
easily
applied
for
phenotyping
plants;
thus,
it
has
great
potential
used
genome
editing
programs
develop
superior
next-generation
crops.
This
review
describes
recent
analytical
tools
techniques
available
study
metabolome,
along
with
significance
sample
preparation
using
targeted
non-targeted
methods.
Advanced
tools,
like
gas
chromatography-mass
spectrometry
(GC-MS),
liquid
chromatography
mass-spectroscopy
(LC-MS),
capillary
electrophoresis-mass
(CE-MS),
fourier
transform
ion
cyclotron
resonance-mass
(FTICR-MS)
matrix-assisted
laser
desorption/ionization
(MALDI),
mobility
(IMS)
nuclear
magnetic
resonance
(NMR)
have
speed
up
precise
metabolic
profiling
Further,
we
provide
complete
overview
bioinformatics
plant
metabolome
database
that
utilized
advance
our
knowledge
biology.
