Molecular Cancer,
Journal Year:
2016,
Volume and Issue:
15(1)
Published: Jan. 6, 2016
As
our
knowledge
of
cancer
metabolism
has
increased,
it
become
apparent
that
metabolic
processes
are
extremely
heterogeneous.
The
reasons
behind
this
heterogeneity
include
genetic
diversity,
the
existence
multiple
and
redundant
pathways,
altered
microenvironmental
conditions,
so
on.
a
result,
methods
in
clinic
beyond
have
been
developed
order
to
image
study
tumor
vivo
vitro
regimes.
Both
regimes
provide
unique
advantages
challenges,
may
be
used
picture
is
spatially
temporally
comprehensive.
Taken
together,
these
hold
key
appropriate
diagnoses
treatments
future.
Metabolomics,
Journal Year:
2018,
Volume and Issue:
14(3)
Published: Feb. 12, 2018
1H
NMR
spectra
from
urine
can
yield
information-rich
data
sets
that
offer
important
insights
into
many
biological
and
biochemical
phenomena.
However,
the
quality
utility
of
these
be
profoundly
affected
by
how
are
processed
interpreted.
For
instance,
if
incorrectly
referenced
or
inconsistently
aligned,
identification
compounds
will
incorrect.
If
mis-phased
baseline
correction
is
flawed,
estimated
concentrations
systematically
biased.
Furthermore,
because
permits
measurement
spanning
up
to
five
orders
magnitude,
several
problems
arise
with
analysis.
signals
originating
most
abundant
metabolites
may
prove
least
biologically
relevant
while
arising
but
hardest
accurately
precisely
measure.
As
a
result,
number
processing
techniques
such
as
scaling,
transformation
normalization
often
required
address
issues.
Therefore,
proper
critical
step
correctly
extract
useful
information
in
any
NMR-based
metabolomic
study.
In
this
review
we
highlight
significance,
advantages
disadvantages
different
spectral
steps
common
studies
urine.
These
include:
chemical
shift
referencing,
phase
correction,
alignment,
binning,
scaling
normalization.
We
also
provide
set
recommendations
for
best
practices
regarding
biofluids,
particular
focus
on
Metabolites,
Journal Year:
2020,
Volume and Issue:
10(2), P. 47 - 47
Published: Jan. 26, 2020
Metabolomic
profiling
of
biofluids,
e.g.,
urine,
plasma,
has
generated
vast
and
ever-increasing
amounts
knowledge
over
the
last
few
decades.
Paradoxically,
metabolomic
analysis
saliva,
most
readily-available
human
biofluid,
lagged.
This
review
explores
history
saliva-based
metabolomics
summarizes
current
salivary
metabolomics.
Current
applications
have
largely
focused
on
diagnostic
biomarker
discovery
value
literature
base
is
explored.
There
also
a
small,
albeit
promising,
concerning
use
in
monitoring
athletic
performance.
Functional
roles
metabolites
remain
unexplored.
Areas
emerging
include
role
oral
host–microbiome
interactions
shaping
metabolite
profile
potential
physiology,
taste
perception.
Discussion
future
research
directions
describes
need
to
begin
acquiring
greater
function
metabolites,
direction
field
gut
metabolome.
The
saliva
as
an
easily
obtainable,
information-rich
fluid
that
could
complement
other
gastrointestinal
fluids
exploration
metabolome
emphasized.
Molecular Cancer,
Journal Year:
2016,
Volume and Issue:
15(1)
Published: Jan. 6, 2016
As
our
knowledge
of
cancer
metabolism
has
increased,
it
become
apparent
that
metabolic
processes
are
extremely
heterogeneous.
The
reasons
behind
this
heterogeneity
include
genetic
diversity,
the
existence
multiple
and
redundant
pathways,
altered
microenvironmental
conditions,
so
on.
a
result,
methods
in
clinic
beyond
have
been
developed
order
to
image
study
tumor
vivo
vitro
regimes.
Both
regimes
provide
unique
advantages
challenges,
may
be
used
picture
is
spatially
temporally
comprehensive.
Taken
together,
these
hold
key
appropriate
diagnoses
treatments
future.
