iScience,
Год журнала:
2025,
Номер
28(2), С. 111729 - 111729
Опубликована: Янв. 2, 2025
The
strobilation
process,
an
asexual
reproduction
mechanism
in
Aurelia
aurita,
transitions
from
the
sessile
polyp
to
pelagic
medusa
stage.
This
study
explored
essential
role
of
microbiome
strobilation,
particularly
through
bacterial
beta
carotene's
impact
on
host's
retinoic
acid
signaling
pathway.
Experiments
demonstrated
that
native
polyps
undergo
normal
while
sterile
exhibit
morphological
defects.
Supplementing
with
provitamin
A
carotene
or
vitamin
metabolite
9-cis
(RA)
remedied
these
defects,
underscoring
their
crucial
strobilation.
Transcriptional
analysis
revealed
and
RA
restored
expression
genes
levels.
Inhibition
key
enzymes
pathway
disrupted
further
confirming
its
importance.
β-carotenoid
synthesis
microbiome,
contrasted
tremendously
reduced
antibiotic-treated
polyps,
emphasizes
microbiome's
pivotal
provision,
facilitating
A.
aurita's
signaling.
Environmental Science Nano,
Год журнала:
2022,
Номер
9(6), С. 2020 - 2031
Опубликована: Янв. 1, 2022
The
combination
of
nanocarrier
delivery
and
genetic
manipulation
significantly
improves
chlorfenapyr
efficiency
has
great
potential
for
sustainable
crop
protection.
Parasites & Vectors,
Год журнала:
2023,
Номер
16(1)
Опубликована: Янв. 26, 2023
The
Asian
tiger
mosquito,
Aedes
albopictus,
is
one
of
the
most
invasive
species
and
a
vector
numerous
arboviruses.
deleterious
effects
long-term
inappropriate
use
chemical
pesticides
have
stimulated
exploration
new,
environmentally
friendly
control
strategies.
Non-coding
RNAs
(ncRNAs)
been
proven
to
participate
in
almost
all
biological
processes
insects.In
this
study,
circular
(circRNAs)
microRNAs
(miRNAs)
covering
five
developmental
stages
[egg,
early
larvae,
late
pupae,
adult
(female
male)]
A.
albopictus
were
obtained
using
whole-transcriptome
sequencing
technology.
Combined
with
long
non-coding
(lncRNAs)
from
previous
research,
circRNA/lncRNA‒miRNA‒mitochondrial
RNA
(mRNA)
networks
constructed.A
total
1434
circRNAs
208
miRNAs
identified.
More
differentially
expressed
(DE
circRNAs)
miRNAs)
found
egg
versus
larvae
comparison
group.
Functional
enrichment
analysis
demonstrated
that
circRNA/lncRNA‒miRNA‒mRNA
involved
chitin
metabolism.
Hub
genes
each
network
screened
out,
which
can
be
used
as
novel
targets
disturb
molting
process
albopictus.Regulatory
relationships
competing
endogenous
(ceRNA)
provide
more
information
manipulate
metamorphosis
are
helpful
for
developing
effective
sustainable
methods
mosquitoes.
Abstract
In
addition
to
preventing
precocious
larval
metamorphosis,
juvenile
hormone
(JH),
synthesized
in
corpora
allata
(CA),
is
known
stimulate
female
reproduction
of
insects.
JH
titer
extremely
low
or
absent
during
but
thereafter
rapidly
increases
the
previtellogenic
stage
and
rises
a
peak
vitellogenic
phase.
However,
mechanisms
underlying
biosynthesis
high
levels
adults
remain
unclear.
We
found
this
study
that
12
genes
involved
synthesis
pathway
were
highly
expressed
CA
adult
locusts.
By
transcriptome
analysis
quantitative
real‐time
–
polymerase
chain
reaction
validation,
total
106
evolutionary
conserved
micro
RNAs
(miRNAs)
163
species‐specific
miRNAs
identified
locust
CA.
Dual‐luciferase
assay
revealed
17
bound
10
(JHSGs)
downregulated
their
expression.
These
stage,
which
was
oppositive
from
targeting
JHSGs.
Six
including
miR‐971‐3p,
miR‐31a,
miR‐9‐5p,
miR‐1‐3p,
miR‐315,
miR‐282
selected
for
function
study.
Co‐application
agomiRs
resulted
significantly
decreased
JHSGs,
accompanied
by
reduced
vitellogenin
expression
as
well
arrested
ovarian
development.
The
data
suggest
multiple
synchronously
at
phase,
thereby
ensuring
JHSG
facilitate
required
JH‐dependent
reproduction.
findings
provide
important
information
deciphering
miRNA—messenger
RNA
modules
regulation
insect
metamorphosis
Abstract
Oogenesis
is
crucial
for
sexual
reproduction
and
provides
the
material
basis
population
continuation.
Nonetheless,
identity
of
cells
involved,
nature
transformation,
underlying
regulators
oogenesis
in
crustaceans
remain
elusive.
Here,
an
atlas
ovary
plotted
via
single‐nuclei
RNA
sequencing
(snRNA‐seq)
mud
crab
Scylla
paramamosain
,
resulting
five
cell
types,
including
germ
cells,
somatic
three
follicle
types
identified,
which
turn
abundant
candidate
markers
them.
Moreover,
profiles
ligand‐receptor
different
indicate
roles
communication
oogenesis.
Dozens
transcription
factors
trajectory
from
oogonia
to
oocytes
as
well
key
molecules/pathways
relevant
are
screened,
evolutionarily
conserved
its
regulatory
mechanism
subject
some
modification
across
various
phyla.
The
spatiotemporal
expression
patterns
seven
further
verified
RNAi
confirms
essential
piwi
VgR
These
data
help
elucidate
gametogenesis
evolution
reproductive
strategy
invertebrates.
iScience,
Год журнала:
2025,
Номер
28(2), С. 111729 - 111729
Опубликована: Янв. 2, 2025
The
strobilation
process,
an
asexual
reproduction
mechanism
in
Aurelia
aurita,
transitions
from
the
sessile
polyp
to
pelagic
medusa
stage.
This
study
explored
essential
role
of
microbiome
strobilation,
particularly
through
bacterial
beta
carotene's
impact
on
host's
retinoic
acid
signaling
pathway.
Experiments
demonstrated
that
native
polyps
undergo
normal
while
sterile
exhibit
morphological
defects.
Supplementing
with
provitamin
A
carotene
or
vitamin
metabolite
9-cis
(RA)
remedied
these
defects,
underscoring
their
crucial
strobilation.
Transcriptional
analysis
revealed
and
RA
restored
expression
genes
levels.
Inhibition
key
enzymes
pathway
disrupted
further
confirming
its
importance.
β-carotenoid
synthesis
microbiome,
contrasted
tremendously
reduced
antibiotic-treated
polyps,
emphasizes
microbiome's
pivotal
provision,
facilitating
A.
aurita's
signaling.
