Recent Advances in Extracellular Vesicles in Amyotrophic Lateral Sclerosis and Emergent Perspectives DOI Creative Commons
Gonçalo J. M. Afonso, Carla Cavaleiro, Jorge Valero

и другие.

Cells, Год журнала: 2023, Номер 12(13), С. 1763 - 1763

Опубликована: Июль 1, 2023

Amyotrophic lateral sclerosis (ALS) is a severe and incurable neurodegenerative disease characterized by the progressive death of motor neurons, leading to paralysis death. It rare high patient-to-patient heterogeneity, which makes its study arduous complex. Extracellular vesicles (EVs) have emerged as important players in development ALS. Thus, ALS phenotype-expressing cells can spread their abnormal bioactive cargo through secretion EVs, even distant tissues. Importantly, owing nature composition, EVs’ formation be exploited for better comprehension this elusive identification novel biomarkers, well potential therapeutic applications, such those based on stem cell-derived exosomes. This review highlights recent advances role EVs etiopathology how promising new strategies.

Язык: Английский

Lipid Metabolism Influence on Neurodegenerative Disease Progression: Is the Vehicle as Important as the Cargo? DOI Creative Commons
Raja Elizabeth Estes, Bernice Lin, Arnav Khera

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2021, Номер 14

Опубликована: Дек. 20, 2021

Many neurodegenerative diseases are characterized by abnormal protein aggregates, including the two most common Alzheimer's disease (AD) and Parkinson's (PD). In global search to prevent treat diseases, research has been focused on early stages of how these pathogenic aggregates initially formed. We argue, however, that an equally important aspect etiology is characteristic spread throughout nervous system, a key process in progression. Growing evidence suggests both alterations lipid metabolism dysregulation extracellular vesicles (EVs) accelerate aggregation progression neurodegeneration, neurons potentially surrounding glia. will review pathways intertwined AD PD. Understanding metabolism, EV biogenesis, uptake regulate could reveal novel therapeutic targets slow or halt

Язык: Английский

Процитировано

53
Exosomal noncoding RNAs in central nervous system diseases: biological functions and potential clinical applications DOI Creative Commons
Zhongyu Wang, Zeng-Jin Wen,

Hai-Ming Xu

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Ноя. 9, 2022

Central nervous system (CNS) disease is a general term for series of complex and diverse diseases, including Alzheimer’s (AD), Parkinson’s (PD), multiple sclerosis (MS), CNS tumors, stroke, epilepsy, amyotrophic lateral (ALS). Interneuron neuron-glia cells communicate with each other through their homeostatic microenvironment. Exosomes in the microenvironment have crucial impacts on interneuron by transferring contents, such as proteins, lipids, ncRNAs, constituting novel form cell-to-cell interaction communication. Exosomal noncoding RNAs (ncRNAs), microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), PIWI-interacting (piRNAs), regulate physiological functions maintain homeostasis. are regarded extracellular messengers that transfer ncRNAs between neurons body fluids due to ability cross blood-brain barrier. This review aims summarize current understanding exosomal prospective diagnostic biomarkers, pathological regulators, therapeutic strategies clinical applications. We also provide an all-sided discussion comparison some similar diseases main limitations challenges

Язык: Английский

Процитировано

36
The role of exosomes in intercellular and inter‐organ communication of the peripheral nervous system DOI Creative Commons

Julia Bischoff,

Alexander Schulz, Helen Morrison

и другие.

FEBS Letters, Год журнала: 2022, Номер 596(5), С. 655 - 664

Опубликована: Янв. 6, 2022

Exosomes, nano-sized extracellular vesicles, are produced via the endosomal pathway and released in space upon fusion of multivesicular bodies with plasma membrane. Recent evidence shows that these vesicles play a key role cell-to-cell communication. Exosomes transport bioactive proteins, mRNAs, microRNA (miRNAs) an active form to adjacent cells or distant organs. In this review, we focus on exosomes peripheral nerve maintenance repair, as well nerve/organ crosstalk, discuss potential benefits exploiting for treating PNS injuries. addition, will highlight emerging new important vehicles physiological systemic crosstalk failures, which could lead organ dysfunction during neuroinflammation aging.

Язык: Английский

Процитировано

34
Huntingtin Co-Isolates with Small Extracellular Vesicles from Blood Plasma of TgHD and KI-HD Pig Models of Huntington’s Disease and Human Blood Plasma DOI Open Access
Hanadi Ananbeh, Jaromir Novak, Štefan Juhás

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(10), С. 5598 - 5598

Опубликована: Май 17, 2022

(1) Background: Huntington’s disease (HD) is rare incurable hereditary neurodegenerative disorder caused by CAG repeat expansion in the gene coding for protein huntingtin (HTT). Mutated (mHTT) undergoes fragmentation and accumulation, affecting cellular functions leading to neuronal cell death. Porcine models of HD are used preclinical testing currently emerging modifying therapies. Such therapies aimed at reducing mHTT expression, postpone onset, slow down progression, point out need biomarkers monitor development therapy efficacy. Recently, extracellular vesicles (EVs), particularly exosomes, gained attention as possible carriers biomarkers. We characterize HTT forms/fragments blood plasma derived EVs transgenic (TgHD) knock-in (KI-HD) porcine models, well patients’ plasma. (2) Methods: Small were isolated ultracentrifugation forms visualized western blotting. (3) Results: The full length 360 kDa co-isolated with from both pig model patient In addition, a ~70 mutant fragment was specific TgHD pigs. Elevated total levels groups compared controls observed patients, however only they significant (p = 0.02). (4) Conclusions: Our study represents valuable initial step towards characterization EV content search

Язык: Английский

Процитировано

29
Extracellular vesicles in neurodegenerative diseases: A systematic review DOI Creative Commons
Alok Raghav, Manish Kumar Singh, Goo‐Bo Jeong

и другие.

Frontiers in Molecular Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Ноя. 24, 2022

Extracellular vesicles (EVs) are known to have a significant role in the central nervous system (CNS) and neurodegenerative disease.PubMed, Scopus, ISI Web of Science, EMBASE, Google Scholar were used identify published articles about EV modifications (2012 Feb 2022).In total, 1,435 papers identified among searched articles, with 1,128 non-duplicate publications being identified. Following screening titles abstracts, 214 excluded; following full-text 93 another 33 excluded. The remaining 60 studies considered. kappa statistic 0.868 indicated that raters highly reliable. Furthermore, inter-reliability intra-reliability coefficients found be 0.931 0.908, respectively, indicating strong reliability consistency between eligible by raters. A total 27 relevant demonstrated EVs as therapeutic diagnostic biomarkers diseases. Of note, 19 14 studies, pioneering roles.EVs play an important (CNS), aiding cell-to-cell communication serving marker target variety home several proteins [including-synuclein (-syn) tau proteins], lipids, genetic materials such DNA RNA. presence novel miRNAs suggests for diagnosis disorders. pathogenesis This systematic review discussed current state EVs' neurological diseases, well some preclinical on potential EVs.

Язык: Английский

Процитировано

29
Mesenchymal Stem Cell-Derived Extracellular Vesicles: An Emerging Diagnostic and Therapeutic Biomolecules for Neurodegenerative Disabilities DOI Creative Commons
Mahmoud Kandeel, Mohamed A. Morsy, Khalid M. Alkhodair

и другие.

Biomolecules, Год журнала: 2023, Номер 13(8), С. 1250 - 1250

Опубликована: Авг. 16, 2023

Mesenchymal stem cells (MSCs) are a type of versatile adult present in various organs. These give rise to extracellular vesicles (EVs) containing diverse array biologically active elements, making them promising approach for therapeutics and diagnostics. This article examines the potential therapeutic applications MSC-derived EVs addressing neurodegenerative disorders such as Alzheimer’s disease (AD), multiple sclerosis (MS), Parkinson’s (PD), amyotrophic lateral (ALS), Huntington’s (HD). Furthermore, state-of-the-art MSC-EV-based therapy AD, HD, PD, ALS, MS is discussed. Significant progress has been made understanding etiology treatments range diseases (NDs) over last few decades. The contents carried across intercellular contact, which often results control recipient cell’s homeostasis. Since represent therapeutically beneficial cargo parent devoid many ethical problems connected with cell-based treatments, they offer viable cell-free alternative tissue regeneration repair. Developing innovative EV-dependent medicines proven difficult due lack standardized procedures EV extraction processes well their pharmacological characteristics mechanisms action. However, recent biotechnology engineering research greatly enhanced content applicability MSC-EVs.

Язык: Английский

Процитировано

16
The panoramic view of amyotrophic lateral sclerosis: A fatal intricate neurological disorder DOI

Swati Dhasmana,

Anupam Dhasmana,

Acharan S. Narula

и другие.

Life Sciences, Год журнала: 2021, Номер 288, С. 120156 - 120156

Опубликована: Ноя. 19, 2021

Язык: Английский

Процитировано

38
Extracellular vesicles from human plasma for biomarkers discovery: Impact of anticoagulants and isolation techniques DOI Creative Commons
Valentina Bettio, Eleonora Mazzucco, Annamaria Antona

и другие.

PLoS ONE, Год журнала: 2023, Номер 18(5), С. e0285440 - e0285440

Опубликована: Май 10, 2023

Extracellular vesicles (EVs) isolated from plasma are increasingly recognized as promising circulating biomarkers for disease discovery and progression, well therapeutic drug delivery. The scientific community underlined the necessity of standard operative procedures isolation storage EVs to ensure robust results. understanding impact pre-analytical variables is still limited some considerations about anticoagulants methods necessary. Therefore, we performed a comparison study between by ultracentrifugation affinity substrate separation EDTA sodium citrate. were characterized Nano Tracking Analysis, Western Blot, cytofluorimetric analysis surface markers, lipidomic analysis. While did not significantly alter any analyzed parameters, influenced size, purity, markers expression profile. Compared ultracentrifugation, yielded bigger particles highly enriched in tetraspanins (CD9, CD63, CD81), fatty acids glycerolipids, with predominant LDL- vLDL-like contamination. Herein, highlighted that method should be carefully evaluated prior design need standardized application discovery.

Язык: Английский

Процитировано

15
Advances in Exosome-Based Therapies for the Repair of Peripheral Nerve Injuries DOI
S. Rahimian, Hossein Najafi, Christine A. Webber

и другие.

Neurochemical Research, Год журнала: 2024, Номер 49(8), С. 1905 - 1925

Опубликована: Май 28, 2024

Язык: Английский

Процитировано

5
Stem Cell-Derived Exosomes as Therapeutic Approach for Neurodegenerative Disorders: From Biology to Biotechnology DOI Creative Commons
Rodrigo Pinheiro Araldi, Fernanda D’Ámelio, Hugo Vigerelli

и другие.

Cells, Год журнала: 2020, Номер 9(12), С. 2663 - 2663

Опубликована: Дек. 11, 2020

The aging population has contributed to the rapid rise in global incidence of neurodegenerative diseases. Despite medical advances, there are no effective treatments for these disorders. Therefore, is an urgent need new In this sense, cell therapy been recognized as best candidate treating incurable diseases, such However, therapeutic use cells can be limited by several factors. Thus, a rediscovery that extracellular vesicles, including exosomes, alternatively explored treatment overcoming limits cell-based therapy. review aims revisit all areas from biology, biogenesis and content biotechnology, proposing minimal information required isolate, characterize, study vesicles scientific and/or clinical purposes.

Язык: Английский

Процитировано

34