Circular RNAs in Viral Infection and Antiviral Treatment DOI Creative Commons
Xin Yin, Hongjun Li,

Yan Zhou

и другие.

Cells, Год журнала: 2024, Номер 13(23), С. 2033 - 2033

Опубликована: Дек. 9, 2024

Circular RNAs (circRNAs) are a class of noncoding that lack the 5′-cap structure and 3′ poly(A) tail. Their distinguishing feature is 5′ ends covalently linked to form closed circular structure. CircRNAs have longer half-life stronger ribonuclease resistance compared with linear RNA. Viral infections lead production circRNA molecules through transcription splicing mechanisms host cells. circRNAs produced from viral genome or reactions cell gene. They participate in regulating replication many viruses, including coronaviruses, human herpesviruses, immunodeficiency virus, cytomegalovirus. regulate infection process by modulating expression cells affect cellular biological processes. Some been proposed as diagnostic markers for infections. In this review, we discussed properties virus-derived circRNAs, functions diverse viruses-derived during infections, critical role host’s antiviral immune defense. Extensive research on applications can help us better understand gene regulatory networks disease mechanisms.

Язык: Английский

An updated database of virus circular RNAs provides new insights into the biogenesis mechanism of the molecule DOI Creative Commons
Ping Fu,

Zena Cai,

Zhiyuan Zhang

и другие.

Emerging Microbes & Infections, Год журнала: 2023, Номер 12(2)

Опубликована: Сен. 19, 2023

Virus circular RNAs (circRNA) have been reported to be extensively expressed and play important roles in viral infections. Previously we build the first database of virus circRNAs named VirusCircBase which has widely used field. This study significantly improved on both data quantity functionality: number circRNAs, species, host organisms was increased from 46440, 23, 9 60859, 43, 22, respectively, 1902 full-length were newly added; new functions added such as visualization expression level Genome Browser. Analysis showed that they had low levels most cells or tissues strong heterogeneity. splicing a much higher proportion non-canonical back-splicing signals compared those animals plants, mainly A5SS (alternative 5' splice site) alternative-splicing. Most no more than two isoforms. Finally, human genes associated with circRNA production investigated 1000 exhibited moderate correlations circRNAs. them negative including 42 encoding RNA-binding proteins. They enriched biological processes related cell cycle RNA processing. Overall, provides valuable resource for further studies also insights into biogenesis mechanisms

Язык: Английский

Процитировано

6
CircRNA: a rising therapeutic strategy for lung injury induced by pulmonary toxicants DOI

Cai Liao,

Zhen-Wei He,

Rui Yu

и другие.

Archives of Toxicology, Год журнала: 2024, Номер 98(5), С. 1297 - 1310

Опубликована: Март 18, 2024

Язык: Английский

Процитировано

2
Circular RNAs as emerging regulators in COVID-19 pathogenesis and progression DOI Creative Commons
Xiaojun Gao, Dan Fang, Yulong Liang

и другие.

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Ноя. 9, 2022

Coronavirus disease 2019 (COVID-19), an infectious acute respiratory caused by a newly emerging RNA virus, is still-growing pandemic that has more than 6 million deaths globally and seriously threatened the lives health of people across world. Currently, several drugs have been used in clinical treatment COVID-19, such as small molecules, neutralizing antibodies, monoclonal antibodies. In addition, vaccines to prevent spread pandemic, adenovirus vector vaccines, inactivated recombinant subunit nucleic acid vaccines. However, efficacy onset adverse reactions vary among individuals. Accumulating evidence demonstrated circular RNAs (circRNAs) are crucial regulators viral infections antiviral immune responses heavily involved COVID-19 pathologies. During novel coronavirus infection, circRNAs not only directly affect transcription process interfere with replication but also indirectly regulate biological processes, including virus-host receptor binding response. Consequently, understanding expression function during severe syndrome 2 (SARS-CoV-2) infection will provide insights into development circRNA-based methods. this review, we summarize recent progress on roles underlying mechanisms inflammatory response, replication, evasion, cytokines induced SARS-CoV-2 thus highlighting diagnostic therapeutic challenges future research directions.

Язык: Английский

Процитировано

9
Building Up a Human Ovarian Antioxidant ceRNA Network, OvAnOx: A Bioinformatic Perspective for Research on Redox-Related Ovarian Functions and Dysfunctions DOI Open Access
Carla Tatone, Giovanna Di Emidio, Rosalia Battaglia

и другие.

Опубликована: Авг. 12, 2024

The ovary is the major determinant of female reproductive health. Ovarian functions are mainly related to primordial follicle pool, which gradually loss with aging. aging and dysfunctions share oxidative stress as common underlying mechanism. ROS signaling essential for normal ovarian processes yet can contribute various disorders when disrupted. Therefore, balance in redox system crucial proper functions. In present study, by focusing on mRNAs ncRNAs described taking into account only validated ncRNA interactions, we built up an antioxidant ceRNA network, namely OvAnOx ceRNA, composed 5 (SOD1, SOD2, CAT, PRDX3, GR), 10 miRNAs lncRNAs (XIST, FGD5-AS1, MALAT1, NEAT1, SNHG1). Our bioinformatic analysis indicated that components not defence, but also involved other Indeed, enzymes encoded mRNA operate within a regulatory network impacts reserve, dynamics, oocyte maturation pathological conditions. represents promising tool unravel complex dialogue between potential signalling pathways health, diseases.

Язык: Английский

Процитировано

1
Genetic alterations in the neuronal development genes are associated with changes of the tumor immune microenvironment in pancreatic cancer DOI Open Access

Kaiyi Mu,

Juan Fu,

Jessica Gai

и другие.

Annals of Pancreatic Cancer, Год журнала: 2023, Номер 6, С. 10 - 10

Опубликована: Ноя. 1, 2023

Background: Pancreatic ductal adenocarcinoma (PDAC) has a poor prognosis and is highly metastatic. Our prior studies have demonstrated the critical role of axon guidance pathway genes in PDAC connection between neuronal development tumor microenvironment. A recent study newly identified 20 [disks large homolog 2 (DLG2), neuron-glial-related cell adhesion molecule (NRCAM), neurexin3 (NRXN3), mitogen-activated protein kinase 10 (MAPK10), platelet-derived growth factor D (PDGFD), C epsilon (PRKCE), potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), polycystic kidney hepatic disease (PKHD1), neural (NCAM1), neuregulin-1 (NRG1), zinc finger 667 (ZNF667), cystic fibrosis transmembrane conductance regulator (CFTR), acyl-CoA medium-chain synthetase-3 (ACSM3), complement 6 (C6), tyrosine phosphatase receptor type (PTPRM), hypoxia-inducible (HIF1A), adenylyl cyclase 5 (ADCY5), adherens junctions-associated (AJAP1), neurobeachin (NBEA), sodium voltage-gated subunit 9 (SCN9A)] that are associated with perineural invasion PDAC. The relationship genetic alterations these immune microenvironment (TME) not previously been investigated.

Язык: Английский

Процитировано

3
RNA-RNA competitive interactions: a molecular civil war ruling cell physiology and diseases DOI Creative Commons
Cristina Barbagallo, Michele Stella, Carmen Ferrara

и другие.

Exploration of Medicine, Год журнала: 2023, Номер unknown, С. 504 - 540

Опубликована: Авг. 31, 2023

The idea that proteins are the main determining factors in functioning of cells and organisms, their dysfunctions first cause pathologies, has been predominant biology biomedicine until recently. This protein-centered view was too simplistic failed to explain physiological pathological complexity cell. About 80% human genome is dynamically pervasively transcribed, mostly as non-protein-coding RNAs (ncRNAs), which competitively interact with each other coding generating a complex RNA network regulating processing, stability, translation and, accordingly, fine-tuning gene expression cells. Qualitative quantitative dysregulations RNA-RNA interaction networks strongly involved onset progression many including cancers degenerative diseases. review will summarize species competitive endogenous network, mechanisms action, involvement phenotypes. Moreover, it give an overview most advanced experimental computational methods dissect rebuild networks.

Язык: Английский

Процитировано

2
Promising Roles of Circular RNAs as Biomarkers and Targets for Potential Diagnosis and Therapy of Tuberculosis DOI Creative Commons
Yifan Huang, Ying Li,

Wensen Lin

и другие.

Biomolecules, Год журнала: 2022, Номер 12(9), С. 1235 - 1235

Опубликована: Сен. 4, 2022

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, remains one of the most threatening infectious diseases worldwide. A series challenges still exist for TB prevention, diagnosis and treatment, which therefore require more attempts to clarify pathological immunological mechanisms in development progression TB. Circular RNAs (circRNAs) are a large class non-coding RNA, mostly expressed eukaryotic cells, generated spliceosome through back-splicing linear RNAs. Accumulating studies have identified that circRNAs widely involved variety physiological processes, acting as sponges or decoys microRNAs proteins, scaffold platforms modulators transcription special templates translation. Due stable spread characteristics circRNAs, they expected serve promising prognostic/diagnostic biomarkers therapeutic targets diseases. In this review, we briefly describe biogenesis, classification, detection technology functions and, particular, outline dynamic, sometimes aberrant changes Moreover, further summarize recent progress research linking TB-related pathogenetic well potential roles diagnostic miRNAs case Mtb is enhance our understanding provide some novel ideas about how overcome associated future.

Язык: Английский

Процитировано

3
Regulatory role of ncRNAs in pulmonary epithelial and endothelial barriers: Molecular therapy clues of influenza-induced acute lung injury DOI
Yi Zhang, Juan Yang, Ping Liu

и другие.

Pharmacological Research, Год журнала: 2022, Номер 185, С. 106509 - 106509

Опубликована: Окт. 13, 2022

Язык: Английский

Процитировано

3
Influenza A virus H1N1-derived circNP37 positively regulates viral replication by sponging host miR-361-5p DOI Creative Commons
Chunyu Zhu, Jingyu Wang,

Yalan Du

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 6, 2023

Summary RNA viruses, such as respiratory syncytial virus and SARS-CoV-2, can generate viral circular RNAs (circRNAs), which may play important roles during infection. However, whether influenza A viruses have this ability to circRNAs remains unknown. In study, we discovered that the negative-strand of H1N1 nucleoprotein (NP) gene a circRNA, designated circNP37. Furthermore, demonstrated circNP37 positively regulated replication by competitively sponging host miR-361-5p inhibited polymerase basic protein 2 (PB2) expression. These results were confirmed using in vivo experiments. Compared with wild-type virus, infection knockout resulted reduced load lungs. This study demonstrates, for first time, existence biological function H1N1-derived findings help us better understand mechanisms pathogenicity. Highlights Negative-strand circRNA CircNP37 plays viral-host interactions regulates eTOC Blurb found virus-derived We also regulate PB2 expression via sponge

Язык: Английский

Процитировано

1
Neuroproteomic Analysis after SARS-CoV-2 Infection Reveals Overrepresented Neurodegeneration Pathways and Disrupted Metabolic Pathways DOI Creative Commons
Indranil Basak,

Rhodri Harfoot,

Jennifer E. Palmer

и другие.

Biomolecules, Год журнала: 2023, Номер 13(11), С. 1597 - 1597

Опубликована: Окт. 30, 2023

Besides respiratory illness, SARS-CoV-2, the causative agent of COVID-19, leads to neurological symptoms. The molecular mechanisms leading neuropathology after SARS-CoV-2 infection are sparsely explored. enters human cells via different receptors, including ACE-2, TMPRSS2, and TMEM106B. In this study, we used a human-induced pluripotent stem cell-derived neuronal model, which expresses TMEM106B, other possible evaluate its susceptibility infection. neurons were exposed followed by RT-qPCR, immunocytochemistry, proteomic analyses infected neurons. Our findings showed that infects at lower rate than cells; however, virus could not replicate or produce infectious virions in model. Despite aborted replication, nuclei irregular morphology compared cells. Since cytokine storm is significant effect COVID-19 patients, addition direct infection, treated with pre-conditioned media from SARS-CoV-2-infected lung cells, neuroproteomic changes investigated. limited profile, particularly affecting mitochondrial proteins apoptotic metabolic pathways, may lead development complications. our study uncover mechanism behind SARS-CoV-2-mediated might contribute lingering effects on brain.

Язык: Английский

Процитировано

1