Obstetric Medicine,
Год журнала:
2024,
Номер
17(3), С. 179 - 183
Опубликована: Июль 25, 2024
Extracellular
vesicles
(EVs)
are
small,
nonreplicating,
lipid-encapsulated
nanoparticles
that
carry
protein
and
nucleic
acid
cargo
derived
from
their
tissue
of
origin.
Due
to
capacity
provide
comparable
insights
solid
organ
biopsy
through
a
minimally
invasive
collection
procedure,
EVs
an
attractive
biomarker
source.
This
review
will
insight,
how
in
circulation
may
novel
way
assess
cholestasis
address
the
possibility
getting
better
understanding
mechanisms
pregnancy
use
serial
hepatic-specific
as
window.
Abstract
Advances
in
technologies
to
isolate
extracellular
vesicles
(EVs)
and
detect/quantify
their
cargo
underpin
the
novel
potential
of
these
circulating
particles
as
a
liquid
biopsy
understand
physiology
disease.
One
organ
particular
interest
terms
utilizing
EVs
is
liver.
The
extent
which
originating
from
liver
reflect
functional
status
this
remains
unknown.
This
an
important
knowledge
gap
that
underpins
utility
derived
biopsy.
primary
objective
study
was
characterize
proteomic
profile
isolated
space
tissue
(LEV)
compare
paired
(LH).
LCMS
analyses
detected
2892
proteins
LEV
2673
LH.
Of
LH,
1547
(58%)
were
also
LEV.
Bioinformatic
demonstrated
comparable
representation
biological
functions
cellular
compartments.
Although,
enriched
membrane
associated
observed
LEV,
while
nuclear
depleted
These
data
support
use
for
organ.
Problems of Virology,
Год журнала:
2023,
Номер
68(3), С. 181 - 197
Опубликована: Июль 6, 2023
Exosomes
are
extracellular
vesicles
of
endosomal
origin,
with
a
bilayer
membrane,
30160
nm
in
diameter.
released
from
cells
different
origins
and
detected
various
body
fluids.
They
contain
nucleic
acids,
proteins,
lipids,
metabolites
can
transfer
the
contents
to
recipient
cells.
Exosome
biogenesis
involves
cellular
proteins
Rab
GTPase
family
ESCRT
system,
which
regulate
budding,
vesicle
transport,
molecule
sorting,
membrane
fusion,
formation
multivesicular
bodies
exosome
secretion.
infected
viruses
may
viral
DNA
RNA,
as
well
mRNA,
microRNA,
other
types
virions.
capable
transferring
components
into
uninfected
organs
tissues.
This
review
analyzes
impact
exosomes
on
life
cycle
widespread
that
cause
serious
human
diseases:
immunodeficiency
virus
(HIV-1),
hepatitis
B
virus,
C
SARS-CoV-2.
Viruses
able
enter
by
endocytosis,
use
molecular
pathways
involving
release
spread
infections.
It
has
been
shown
have
multidirectional
effects
pathogenesis
infections,
suppressing
or
enhancing
course
diseases.
potentially
be
used
noninvasive
diagnostics
biomarkers
stage
infection,
loaded
biomolecules
drugs
-
therapeutic
agents.
Genetically
modified
promising
candidates
for
new
antiviral
vaccines.Экзосомы
внеклеточные
везикулы
эндосомального
происхождения
c
двухслойной
мембраной,
диаметром
нм.
Экзосомы
высвобождаются
из
клеток
разного
и
определяются
в
различных
биологических
жидкостях
организма.
Они
содержат
клеточные
нуклеиновые
кислоты,
белки,
липиды,
метаболиты
могут
передавать
содержимое
клеткам-реципиентам.
В
биогенезе
экзосом
участвуют
белки
семейства
ГТФаз
системы
ESCRT,
которые
регулируют
почкование,
транспорт
везикул,
сортировку
молекул,
слияние
мембран,
образование
мультивезикулярных
телец
секрецию
экзосом.
Из
клеток,
инфицированных
вирусами,
экзосомы,
геномные
вирусные
ДНК
РНК,
а
также
мРНК,
микроРНК,
другие
виды
вирионы.
способны
переносить
компоненты
неинфицированные
клетки
органов
тканей.
настоящем
обзоре
проанализировано
влияние
на
жизненный
цикл
широко
распространённых
вирусов,
вызывающих
серьёзные
заболевания
человека:
вирус
иммунодефицита
человека
1-го
типа,
гепатита
В,
С,
Вирусы
проникать
путём
эндоцитоза,
используют
молекулярные
пути
с
участием
белков
для
высвобождения
распространения
вирусных
инфекций.
Показано,
что
экзосомы
оказывать
разнонаправленные
действия
патогенез
инфекций,
подавляя
или
способствуя
развитию
вызываемых
ими
заболеваний.
потенциально
использоваться
неинвазивной
диагностике
как
биомаркеры
стадии
инфекции,
нагруженные
биомолекулами
лекарственными
препаратами,
терапевтические
средства.
Генетически
модифицированные
перспективные
кандидаты
новых
противовирусных
вакцин.
Non-alcoholic
fatty
liver
disease
(NAFLD)
is
a
disorder
that
has
become
global
health
concern
due
to
its
increasing
prevalence.
Currently,
there
need
for
reliable
biomarkers
aid
in
the
diagnosis
and
prognosis
of
NAFLD.
Extracellular
vesicles
(EVs)
are
promising
candidates
biomarker
discovery,
as
they
carry
proteins
reflect
pathophysiological
state
liver.
In
this
review,
we
developed
list
EV
could
be
used
diagnostic
We
employed
multi-step
strategy
involved
reviewing
comparing
various
sources
information.
Firstly,
reviewed
papers
have
studied
EVs
NAFLD,
well
circulating
To
further
identify
potential
candidates,
utilized
database
Vesiclepedia.org
qualify
each
protein.
Finally,
consulted
Human
Protein
Atlas
search
candidates'
localization,
focusing
on
membrane
proteins.
By
integrating
these
information,
comprehensive
protein
monitoring
conclusion,
our
identifying
EV-based
NAFLD
provides
approach
can
also
applied
other
diseases.
The
identified
through
significant
implications
development
non-invasive
tests
improve
management
treatment
prevalent
disorder.
Obstetric Medicine,
Год журнала:
2024,
Номер
17(3), С. 179 - 183
Опубликована: Июль 25, 2024
Extracellular
vesicles
(EVs)
are
small,
nonreplicating,
lipid-encapsulated
nanoparticles
that
carry
protein
and
nucleic
acid
cargo
derived
from
their
tissue
of
origin.
Due
to
capacity
provide
comparable
insights
solid
organ
biopsy
through
a
minimally
invasive
collection
procedure,
EVs
an
attractive
biomarker
source.
This
review
will
insight,
how
in
circulation
may
novel
way
assess
cholestasis
address
the
possibility
getting
better
understanding
mechanisms
pregnancy
use
serial
hepatic-specific
as
window.
