Journal of Proteome Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 9, 2024
Single-cell
analysis
is
an
active
area
of
research
in
many
fields
biology.
Measurements
at
single-cell
resolution
allow
researchers
to
study
diverse
populations
without
losing
biologically
meaningful
information
sample
averages.
Many
technologies
have
been
used
single
cells,
including
mass
spectrometry-based
proteomics
(SCP).
SCP
has
seen
a
lot
growth
over
the
past
couple
years
through
improvements
data
acquisition
and
analysis,
leading
greater
proteomic
depth.
Because
method
development
main
focus
SCP,
biological
applications
sprinkled
only
as
proof-of-concept.
However,
methods
now
provide
significant
coverage
proteome
implemented
laboratories.
Thus,
primary
question
address
our
community
whether
current
state
technology
ready
for
widespread
adoption
inquiry.
In
this
Perspective,
we
examine
potential
three
thematic
areas
investigation:
cell
annotation,
developmental
trajectories,
spatial
mapping.
We
identify
that
limitation
throughput.
As
depth
target
date,
advocate
change
facilitate
measuring
tens
thousands
proteomes
enable
beyond
ABSTRACT
Single‐cell
proteomics
(SCP)
has
advanced
significantly
in
recent
years,
with
new
tools
specifically
designed
for
the
preparation
and
analysis
of
single
cells
now
commercially
available
to
researchers.
The
field
is
sufficiently
mature
be
broadly
accessible
any
lab
capable
isolating
performing
bulk‐scale
proteomic
analyses.
In
this
review,
we
highlight
work
SCP
that
lowered
barrier
entry,
thus
providing
a
practical
guide
those
who
are
newly
entering
field.
We
outline
fundamental
principles
report
multiple
paths
accomplish
key
steps
successful
experiment
including
sample
preparation,
separation,
mass
spectrometry
data
acquisition
analysis.
recommend
researchers
start
label‐free
workflow,
as
achieving
high‐quality
quantitatively
accurate
results
more
straightforward
than
label‐based
multiplexed
strategies.
By
leveraging
these
means,
can
confidently
perform
experiments
make
meaningful
discoveries
at
single‐cell
level.
PROTEOMICS - CLINICAL APPLICATIONS,
Год журнала:
2024,
Номер
19(1)
Опубликована: Ноя. 21, 2024
ABSTRACT
Background
Triple‐negative
breast
cancer
(TNBC)
is
an
aggressive
and
complex
subtype
of
characterized
by
a
lack
targeted
treatment
options.
Intratumoral
heterogeneity
significantly
drives
disease
progression
complicates
therapeutic
responses,
necessitating
advanced
analytical
approaches
to
understand
its
underlying
biology.
This
review
aims
explore
the
advancements
in
single‐cell
proteomics
their
application
uncovering
cellular
diversity
TNBC.
It
highlights
innovations
sample
preparation,
mass
spectrometry‐based
techniques,
potential
for
integrating
into
multi‐omics
platforms.
Methods
The
discusses
combination
improved
preparation
methods
cutting‐edge
spectrometry
techniques
proteomics.
emphasizes
challenges
associated
with
protein
analysis,
such
as
inability
amplify
proteins
akin
transcripts,
examines
strategies
overcome
these
limitations.
Results
Single‐cell
provides
direct
link
phenotype
cell
behavior,
complementing
transcriptomic
offering
new
insights
mechanisms
driving
integration
has
enabled
deeper
exploration
mechanisms.
Conclusion
Despite
challenges,
holds
immense
evolve
high‐throughput
scalable
platform.
Addressing
existing
hurdles
will
enable
biological
insights,
ultimately
enhancing
diagnosis
European Journal of Translational Myology,
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 16, 2023
Duchenne
muscular
dystrophy
is
a
highly
progressive
muscle
wasting
disease
of
early
childhood
and
characterized
by
complex
pathophysiological
histopathological
changes
in
the
voluntary
contractile
system,
including
myonecrosis,
chronic
inflammation,
fat
substitution
reactive
myofibrosis.
The
continued
loss
functional
myofibres
replacement
with
non-contractile
cells,
as
well
extensive
tissue
scarring
decline
elasticity,
leads
to
severe
skeletal
weakness.
In
addition,
dystrophic
muscles
exhibit
greatly
diminished
regenerative
capacity
counteract
ongoing
process
fibre
degeneration.
normal
tissues,
an
abundant
stem
cell
pool
consisting
satellite
cells
that
are
localized
between
sarcolemma
basal
lamina,
provides
rich
source
for
production
activated
myogenic
progenitor
involved
efficient
myofibre
repair
regeneration.
Interestingly,
self-renewal
maintaining
essential
matured
increased
dystrophin-deficient
fibres.
However,
hyperplasia
does
not
result
recovery
due
impaired
asymmetric
divisions.
lack
expression
full-length
dystrophin
isoform
Dp427-M,
which
primary
defects
DMD
gene,
appears
affect
key
regulators
polarity
causing
reduced
differentiation
progenitors,
This
review
outlines
complexity
dystrophinopathy
describes
importance
role
dysfunction.
A
brief
discussion
bioanalytical
usefulness
single
proteomics
future
studies
biology
provided.
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 8, 2024
Functional
selectivity
refers
to
the
activation
of
differential
signalling
and
cellular
outputs
downstream
same
membrane-bound
receptor
when
activated
by
two
or
more
different
ligands.
has
been
described
extensively
studied
for
G-protein
Coupled
Receptors
(GPCRs),
leading
specific
therapeutic
options
dysregulated
GPCRs
functions.
However,
studies
regarding
functional
Receptor
Tyrosine
Kinases
(RTKs)
remain
sparse.
Here,
we
will
summarize
recent
data
about
RTK
focusing
on
how
nature
amount
ligands
crosstalk
RTKs
with
other
membrane
proteins
regulate
specificity
signalling.
In
addition,
discuss
structural
changes
in
upon
ligand
binding
affects
selective
pathways.
Much
remains
be
known
integration
signals
affecting
orchestrate
long-term
outcomes.
Recent
advancements
omics,
specifically
quantitative
phosphoproteomics,
systems
biology
methods
study,
model
integrate
types
large-scale
omics
have
increased
our
ability
compare
several
a
global,
system-wide
fashion.
We
such
facilitate
exploration
important
hubs
enable
data-driven
predictions
aiming
at
improving
efficacy
therapeutics
diseases
like
cancer,
where
redundant
pathways
often
compromise
treatment
efficacy.
Journal of Proteome Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 9, 2024
Single-cell
analysis
is
an
active
area
of
research
in
many
fields
biology.
Measurements
at
single-cell
resolution
allow
researchers
to
study
diverse
populations
without
losing
biologically
meaningful
information
sample
averages.
Many
technologies
have
been
used
single
cells,
including
mass
spectrometry-based
proteomics
(SCP).
SCP
has
seen
a
lot
growth
over
the
past
couple
years
through
improvements
data
acquisition
and
analysis,
leading
greater
proteomic
depth.
Because
method
development
main
focus
SCP,
biological
applications
sprinkled
only
as
proof-of-concept.
However,
methods
now
provide
significant
coverage
proteome
implemented
laboratories.
Thus,
primary
question
address
our
community
whether
current
state
technology
ready
for
widespread
adoption
inquiry.
In
this
Perspective,
we
examine
potential
three
thematic
areas
investigation:
cell
annotation,
developmental
trajectories,
spatial
mapping.
We
identify
that
limitation
throughput.
As
depth
target
date,
advocate
change
facilitate
measuring
tens
thousands
proteomes
enable
beyond
