Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Май 21, 2025
Sulfonamide
resistance
genes
(
sul
genes)
have
a
high
detection
rate
and
strong
transmissibility.
Therefore,
there
is
an
urgent
need
to
develop
more
efficient
methods
enhance
the
monitoring
of
genes.
Current
analytical
are
insufficient
for
simultaneous
accurate
quantification
all
sulfonamides
To
overcome
this
limitation,
quadruple
method
was
established
by
integrating
droplet
digital
PCR
(ddPCR)
with
ratio-based
probe-mixing
strategy,
achieving
sensitive
sul1
,
sul2
sul3
sul4
in
diverse
matrices.
Correspondingly,
primers
probes
were
meticulously
designed
rigorously
validated,
critical
parameters
ddPCR
such
as
annealing
temperature,
concentrations
systematically
optimized.
As
results,
demonstrates
excellent
sensitivity
limits
(LOD)
ranging
from
3.98
6.16
copies/reaction,
good
repeatability
(coefficient
variation
<25%),
adequately
meeting
requirement
quantification.
Furthermore,
new
applied
across
115
samples,
including
human
feces,
animal-derived
foods,
sewage
surface
water,
positive
rates
100%
99.13%
93.91%
68.70%
concentration
non-detection
2.14
×
10
9
copies/g.
In
summary,
developed
has
potential
serve
tool
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(6), С. 5777 - 5777
Опубликована: Март 17, 2023
Despite
the
undisputed
development
of
medicine,
antibiotics
still
serve
as
first-choice
drugs
for
patients
with
infectious
disorders.
The
widespread
use
results
from
a
wide
spectrum
their
actions
encompassing
mechanisms
responsible
for:
inhibition
bacterial
cell
wall
biosynthesis,
disruption
membrane
integrity,
suppression
nucleic
acids
and/or
proteins
synthesis,
well
disturbances
metabolic
processes.
However,
availability
antibiotics,
accompanied
by
overprescription,
acts
double-edged
sword,
since
overuse
misuse
leads
to
growing
number
multidrug-resistant
microbes.
This,
in
turn,
has
recently
emerged
global
public
health
challenge
facing
both
clinicians
and
patients.
In
addition
intrinsic
resistance,
bacteria
can
acquire
resistance
particular
antimicrobial
agents
through
transfer
genetic
material
conferring
resistance.
Amongst
most
common
strategies
are:
drug
target
site
changes,
increased
permeability
antibiotic
inactivation,
efflux
pumps.
A
better
understanding
interplay
between
defense
against
is
crucial
developing
new
or
combinations.
Herein,
we
provide
brief
overview
current
nanomedicine-based
that
aim
improve
efficacy
antibiotics.
Antibiotics,
Год журнала:
2021,
Номер
10(11), С. 1314 - 1314
Опубликована: Окт. 28, 2021
Tetracyclines
and
sulfonamides
are
broad-spectrum
antibacterial
agents
which
have
been
used
to
treat
bacterial
infections
for
over
half
a
century.
The
widespread
use
of
tetracyclines
led
the
emergence
resistance
in
diverse
group
bacteria.
This
can
be
studied
by
searching
genes
present
bacteria
responsible
different
mechanisms.
Salmonella
is
one
leading
causing
foodborne
diseases
worldwide,
its
has
widely
reported.
literature
review
searched
Virtual
Health
Library
articles
with
specific
data
samples:
found,
primers
PCR,
thermocycler
conditions.
results
revealed
that
presented
high
rates
tetracycline
sulfonamide,
most
frequent
samples
isolate
were
poultry
pork.
frequently
detected
from
spp.
tetA
followed
tetB.
gene
sul1
sul2
sulfonamide
Salmonella.
These
associated
plasmids,
transposons,
or
both,
often
conjugative,
highlighting
transference
potential
these
other
bacteria,
environments,
animals,
humans.
ACS Infectious Diseases,
Год журнала:
2024,
Номер
10(5), С. 1483 - 1519
Опубликована: Май 1, 2024
The
development
of
effective
antibacterial
solutions
has
become
paramount
in
maintaining
global
health
this
era
increasing
bacterial
threats
and
rampant
antibiotic
resistance.
Traditional
antibiotics
have
played
a
significant
role
combating
infections
throughout
history.
However,
the
emergence
novel
resistant
strains
necessitates
constant
innovation
research.
We
analyzed
data
on
antibacterials
from
CAS
Content
Collection,
largest
human-curated
collection
published
scientific
knowledge,
which
proven
valuable
for
quantitative
analysis
knowledge.
Our
focuses
mining
Collection
recent
publications
(since
2012).
This
article
aims
to
explore
intricate
landscape
research
while
reviewing
advancement
traditional
emerging
strategies.
By
delving
into
resistance
mechanisms,
paper
highlights
need
find
alternate
strategies
address
growing
concern.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Май 14, 2024
In
this
study,
we
synthesized
new
series
of
5-oxo-2-phenyl-4-(arylsulfamoyl)sulphenyl)
hydrazono)-4,5-dihydro-1H-pyrrole-3-carboxylate
hybrids
4a-f
with
the
goal
overcoming
sulfonamide
resistance
and
identifying
novel
therapeutic
candidates
by
chemical
changes.
The
structures
were
established
over
spectroscopic
tools.
frontier
molecular
orbitals
configuration
energetic
possessions
compounds
discovered
utilizing
DFT/B3LYP/6-311++
G**
procedure.
3D
plots
both
HOMO
LUMO
showed
comparable
led
to
close
values
their
energies.
Amongst
prepared
analogues,
4a-f,
hybrid
4a
presented
potent
inhibitory
towards
S.
typhimurium
(IZD
=
15
mm,
MIC
19.24
µg/mL)
significant
inhibition
19
11.31
against
E.coli
in
contrast
(Sulfamethoxazole)
reference
Whereas,
4d
demonstrated
16
enhanced
E.
Coli,
Additionally,
generated
analogues''
docking
was
estimated
(PDB:
3TZF
6CLV)
proteins.
Analogue
4e
had
highest
documented
binding
score
as
soon
linked
other
analogues.
consequences
fitting
addressed
antibacterial
valuation.
Reviews in Aquaculture,
Год журнала:
2025,
Номер
17(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Vibrio
parahaemolyticus
,
a
ubiquitous
pathogen
in
marine
and
estuarine
environments,
is
foodborne
microorganism
whose
infections
have
become
global
public
health
challenge.
In
recent
years,
the
overuse
misuse
of
antibiotics
led
to
emergence
increasing
numbers
antibiotic‐resistant
strains
V.
worldwide.
These
harbor
resistance
genes
that
mediate
various
mechanisms,
presenting
significant
challenge
antibiotic
therapy.
Furthermore,
horizontal
gene
transfer
(HGT)
accelerating
impacts
climate
change
facilitated
formation
spread
resistance,
contributing
proliferation
resistant
exacerbating
threat.
This
review
aims
provide
comprehensive
overview
mechanisms
effects
on
its
strategies
curb
transmission
strains.
Additionally,
we
conducted
statistical
analysis
data
from
6727
isolates
obtained
27
countries
across
Asia,
Africa,
Americas,
Europe,
through
literature
review,
better
understand
distribution
trends
this
pathogen.
We
hope
study
will
serve
as
an
important
reference
for
controlling
addressing
critical
issue.
