Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(36), С. 13244 - 13254
Опубликована: Авг. 30, 2023
Beauveria
bassiana
acts
as
an
endophytic
fungus
that
controls
herbivorous
pests
by
stimulating
plant
defenses
and
inducing
systemic
resistance.
Through
multiomics
analysis,
325
differential
metabolites
1739
expressed
genes
were
observed
in
tomatoes
treated
with
B.
root
irrigation;
meanwhile,
152
1002
local
leaf
spraying.
Among
the
upregulated
α-solanine,
5-O-caffeoylshikimic
acid,
clerodendrin
A,
peucedanin,
which
demonstrated
anti-insect
activity.
These
primarily
associated
alkaloid
biosynthesis,
flavonoid
tryptophan
metabolism
pathways.
Furthermore,
gene
silencing
of
UDP-glucose:sterol
glucosyltransferase,
a
involved
α-solanine
synthesis,
indicated
could
inhibit
reproduction
whiteflies
regulating
α-solanine.
This
study
highlighted
ability
to
modulate
secondary
emphasized
significance
understanding
harnessing
multitrophic
interactions
for
sustainable
agriculture.
Abstract
Some
herbivore-induced-plant
volatiles
(HIPVs)
compounds
are
vital
for
the
functioning
of
an
ecosystem,
by
triggering
multi-trophic
interactions
natural
enemies,
plants
and
herbivores.
However,
effect
these
chemicals,
which
play
a
crucial
role
in
regulating
between
plant-herbivore-entomopathogenic
fungi,
is
still
unknown.
To
fill
this
scientific
gap,
we
therefore
investigated
how
chemicals
influence
entomopathogenic
fungi
growth
efficacy.
In
study,
Lipaphis
erysimi
induced
Arabidopsis
thaliana
HIPVs
were
collected
using
headspace
system
detected
with
GC-MS,
then
analyzed
effects
on
Lecanicillium
lecanii
strain
V3450.
We
found
that
menthol
methyl
salicylate
at
1
10
nmol·ml
−1
improved
many
performance
aspects
fungus,
such
as
germination,
sporulation,
appressorial
formation
well
its
pathogenicity
virulence.
These
findings
not
only
important
understanding
but
also
would
contribute
developing
new
easier
procedures
conidial
mass
production
improve
virulence
biological
pest
management
strategies.
Scientific Reports,
Год журнала:
2021,
Номер
11(1)
Опубликована: Март 2, 2021
The
tick
Rhipicephalus
microplus
poses
a
serious
threat
to
the
cattle
industry,
resulting
in
economic
losses
aggravated
by
resistance
chemical
acaricides.
Strains
of
Metarhizium
spp.,
well-known
group
entomopathogenic
fungi,
can
contribute
managing
this
ectoparasite.
We
explored
two
novel
granular,
microsclerotia-
or
blastospores-based
formulations
robertsii
for
R.
control
under
semi-field
conditions.
Fungal
persistence
soil
was
also
observed
336
days.
experiment
used
pots
Urochloa
decumbens
cv.
Basilisk
grass,
treated
with
0.25
0.5
mg
granular
formulation/cm
Fungal Biology,
Год журнала:
2024,
Номер
128(2), С. 1643 - 1656
Опубликована: Янв. 24, 2024
Microsclerotia
(MS)
are
considered
one
of
the
most
promising
propagules
for
use
as
active
ingredients
in
biopesticides
due
to
their
tolerance
abiotic
factors
and
ability
produce
infective
conidia
control
insect
pests
plant
pathogens.
Therefore,
main
objective
this
research
was
establish
conditions
required
induce
formation
microsclerotia
Metarhizium
robertsii
Mt004
study
its
development
process,
insecticidal
activity
MS-derived
conidia.
M.
started
form
hyphal
aggregates
after
2
days,
structures
looked
more
compact
8
MS
were
mature
pigmented
20
days.
The
final
yield
2.0
×
103
MS/mL,
size
varied
between
356.9
1348.4
μm.
Ultrastructure
analysis
revealed
that
internal
cells
lyse
during
maturation
contained
only
a
few
live
embedded
an
extracellular
matrix.
Conidia
produced
by
Solid
State
Fermentation
(SSF)
on
rice
completely
died
1
h
irradiation
with
UV-B
light,
while
84.7%
remained
viable
4
h.
2.5
times
tolerant
heat
than
conidia,
when
exposed
3
at
50
°C.
Storage
trials
showed
viability
rapidly
dropped
months,
months
storage
°C
18
virulent
grains
against
Diatraea
saccharalis
larvae.
Mature
radiation
from
SSF.
fermentation
process
requires
optimization
increase
reducing
time
maintaining
robustness.
In
general,
these
results
led
conclusion
persistent
efficient
harsh
environmental
conditions.
Our
findings
provide
baseline
production
better
understanding
strains.
Environmental Microbiology,
Год журнала:
2017,
Номер
19(10), С. 4365 - 4378
Опубликована: Сен. 19, 2017
Microbial
pathogens
are
exposed
to
damaging
reactive
oxygen
species
(ROS)
produced
from
a
variety
of
sources
including
chemical
reactions
due
exposure
stress
(UV,
heat)
or
by
hosts
as
defense
response.
Here,
we
demonstrate
that
bifunctional
catalase-peroxidase,
MakatG1,
in
the
locust-specific
fungal
pathogen,
Metarhizium
acridum,
functions
ROS
detoxification
mechanism
during
host
cuticle
penetration.
MakatG1
expression
was
highly
induced
on-cuticle
appressoria
development
compared
vegetative
(mycelia)
growth
vivo
insect
hemocoel.
A
deletion
mutant
strain
(ΔMakatG1)
showed
decreased
catalase
and
peroxidase
activities
significantly
increased
susceptibility
oxidative
(H2
O2
menadione)
UV
wild-type
complemented
strains.
Insect
bioassays
revealed
reduced
virulence
ΔMakatG1
when
topically
inoculated,
but
no
impairment
bypassed.
Germination
formation
rates
for
were
on
locust
wings
quinone/phenolic
compounds
derived
wings,
not
affected
plastic
surfaces
with
strain.
These
data
indicate
plays
pivotal
role
penetration,
reacting
detoxifying
specific
cuticular
present
early
stages
infection.
PLoS ONE,
Год журнала:
2019,
Номер
14(2), С. e0211457 - e0211457
Опубликована: Фев. 1, 2019
The
use
of
Beauveria
bassiana
in
biological
control
agricultural
pests
is
mainly
hampered
by
environmental
factors,
such
as
elevated
temperatures
and
low
humidity.
These
limitations,
further
amplified
a
global
warming
scenario,
could
nullify
strategies
based
on
this
fungus.
identification
thermotolerant
B.
isolates
represents
possible
strategy
to
overcome
problem.
In
study,
order
maximize
the
probability
isolation
bassiana,
soil
samples
infected
insects
were
collected
warm
areas
Syria.
obtained
fungal
tested
for
different
parameters
(i.e.,
growth
rate,
sporulation
spore
germination)
at
growing
ranging
from
20°C
35°C.
Among
these
(eight
11
samples),
five
with
highest
production
germination
30°C
their
entomopathogenicity
through
vivo
assays
Ephestia
kuehniella
larvae.
Insect
mortality
induced
ranged
31%
100%.
Two
isolates,
one
Phyllognathus
excavatus
soil,
caused
50%
larval
less
than
four
days,
reaching
values
exceeding
92%
ten
days.
two
molecularly
identified
sensu
stricto
using
three
markers
ITS,
Bloc
EF1-α).
Considering
promising
results,
studies
are
ongoing,
testing
efficiency
field
conditions
agents
insect
Mediterranean
Subtropical
regions.
