Microsclerotia from Metarhizium robertsii: Production, ultrastructural analysis, robustness, and insecticidal activity DOI Creative Commons
Jennifer Lorena García Riaño, Gloria Barrera, Leonardo Castellanos

et al.

Fungal Biology, Journal Year: 2024, Volume and Issue: 128(2), P. 1643 - 1656

Published: Jan. 24, 2024

Microsclerotia (MS) are considered one of the most promising propagules for use as active ingredients in biopesticides due to their tolerance abiotic factors and ability produce infective conidia control insect pests plant pathogens. Therefore, main objective this research was establish conditions required induce formation microsclerotia Metarhizium robertsii Mt004 study its development process, insecticidal activity MS-derived conidia. M. started form hyphal aggregates after 2 days, structures looked more compact 8 MS were mature pigmented 20 days. The final yield 2.0 × 103 MS/mL, size varied between 356.9 1348.4 μm. Ultrastructure analysis revealed that internal cells lyse during maturation contained only a few live embedded an extracellular matrix. Conidia produced by Solid State Fermentation (SSF) on rice completely died 1 h irradiation with UV-B light, while 84.7% remained viable 4 h. 2.5 times tolerant heat than conidia, when exposed 3 at 50 °C. Storage trials showed viability rapidly dropped months, months storage °C 18 virulent grains against Diatraea saccharalis larvae. Mature radiation from SSF. fermentation process requires optimization increase reducing time maintaining robustness. In general, these results led conclusion persistent efficient harsh environmental conditions. Our findings provide baseline production better understanding strains.

Language: Английский

The Herbivore-Induced Plant Volatiles Methyl Salicylate and Menthol Positively affect Growth and Pathogenicity of Entomopathogenic Fungi DOI Creative Commons
Yongwen Lin, Muhammad Qasim, Mubasher Hussain

et al.

Scientific Reports, Journal Year: 2017, Volume and Issue: 7(1)

Published: Jan. 12, 2017

Abstract Some herbivore-induced-plant volatiles (HIPVs) compounds are vital for the functioning of an ecosystem, by triggering multi-trophic interactions natural enemies, plants and herbivores. However, effect these chemicals, which play a crucial role in regulating between plant-herbivore-entomopathogenic fungi, is still unknown. To fill this scientific gap, we therefore investigated how chemicals influence entomopathogenic fungi growth efficacy. In study, Lipaphis erysimi induced Arabidopsis thaliana HIPVs were collected using headspace system detected with GC-MS, then analyzed effects on Lecanicillium lecanii strain V3450. We found that menthol methyl salicylate at 1 10 nmol·ml −1 improved many performance aspects fungus, such as germination, sporulation, appressorial formation well its pathogenicity virulence. These findings not only important understanding but also would contribute developing new easier procedures conidial mass production improve virulence biological pest management strategies.

Language: Английский

Citations

49
Phenotypic and molecular insights into heat tolerance of formulated cells as active ingredients of fungal insecticides DOI

Sen‐Miao Tong,

Ming‐Guang Feng

Applied Microbiology and Biotechnology, Journal Year: 2020, Volume and Issue: 104(13), P. 5711 - 5724

Published: May 13, 2020

Language: Английский

Citations

42
Innovative granular formulation of Metarhizium robertsii microsclerotia and blastospores for cattle tick control DOI Creative Commons

Allan Felipe Marciano,

Gabriel Moura Mascarin,

Renato Felipe Ferreira Franco

et al.

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: March 2, 2021

The tick Rhipicephalus microplus poses a serious threat to the cattle industry, resulting in economic losses aggravated by resistance chemical acaricides. Strains of Metarhizium spp., well-known group entomopathogenic fungi, can contribute managing this ectoparasite. We explored two novel granular, microsclerotia- or blastospores-based formulations robertsii for R. control under semi-field conditions. Fungal persistence soil was also observed 336 days. experiment used pots Urochloa decumbens cv. Basilisk grass, treated with 0.25 0.5 mg granular formulation/cm

Language: Английский

Citations

38
Molecular basis and regulatory mechanisms underlying fungal insecticides' resistance to solar ultraviolet irradiation DOI

Sen‐Miao Tong,

Ming‐Guang Feng

Pest Management Science, Journal Year: 2021, Volume and Issue: 78(1), P. 30 - 42

Published: Aug. 16, 2021

Abstract Resistance to solar ultraviolet (UV) irradiation is crucial for field‐persistent control efficacies of fungal formulations against arthropod pests, because their active ingredients are formulated conidia very sensitive UV wavelengths. This review seeks summarize advances in studies aiming quantify, understand and improve conidial resistance. One focus has been on the many sets genes that have revealed postgenomic era contribute or mediate resistance insect pathogens serving as main sources insecticides. Such genetic unveiled broad basis UV‐resistant molecules including cytosolic solutes, cell wall components, various antioxidant enzymes, numerous effectors signaling proteins, function developmental, biosynthetic stress‐responsive pathways. Another molecular regulatory mechanisms underlying photorepair UV‐induced DNA lesions photoreactivation UV‐impaired conidia. Studies shed light upon a photoprotective mechanism depending not only one two photorepair‐required photolyases, but also white collar proteins other partners play similar more important roles via interactions with photolyases. Research hotspots suggested explore network photoprotection development application strategies © 2021 Society Chemical Industry.

Language: Английский

Citations

38
Evaluation of virulence, tolerance to environmental factors and antimicrobial activities of entomopathogenic fungi against two-spotted spider mite, Tetranychus urticae DOI
Tae Young Shin, Sung Min Bae,

Dong Jun Kim

et al.

Mycoscience, Journal Year: 2017, Volume and Issue: 58(3), P. 204 - 212

Published: March 8, 2017

Language: Английский

Citations

44
A bifunctional catalase‐peroxidase, MakatG1, contributes to virulence of Metarhizium acridum by overcoming oxidative stress on the host insect cuticle DOI
Guo-Hong Li,

Anni Fan,

Guoxiong Peng

et al.

Environmental Microbiology, Journal Year: 2017, Volume and Issue: 19(10), P. 4365 - 4378

Published: Sept. 19, 2017

Microbial pathogens are exposed to damaging reactive oxygen species (ROS) produced from a variety of sources including chemical reactions due exposure stress (UV, heat) or by hosts as defense response. Here, we demonstrate that bifunctional catalase-peroxidase, MakatG1, in the locust-specific fungal pathogen, Metarhizium acridum, functions ROS detoxification mechanism during host cuticle penetration. MakatG1 expression was highly induced on-cuticle appressoria development compared vegetative (mycelia) growth vivo insect hemocoel. A deletion mutant strain (ΔMakatG1) showed decreased catalase and peroxidase activities significantly increased susceptibility oxidative (H2 O2 menadione) UV wild-type complemented strains. Insect bioassays revealed reduced virulence ΔMakatG1 when topically inoculated, but no impairment bypassed. Germination formation rates for were on locust wings quinone/phenolic compounds derived wings, not affected plastic surfaces with strain. These data indicate plays pivotal role penetration, reacting detoxifying specific cuticular present early stages infection.

Language: Английский

Citations

40
UV sensitivity of Beauveria bassiana and Metarhizium anisopliae isolates under investigation as potential biological control agents in South African citrus orchards DOI
Mavis A. Acheampong, Martin Hill, Sean D. Moore

et al.

Fungal Biology, Journal Year: 2019, Volume and Issue: 124(5), P. 304 - 310

Published: Sept. 6, 2019

Language: Английский

Citations

39
Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates DOI Creative Commons
Sumer Alali,

Valeria Mereghetti,

Franco Faoro

et al.

PLoS ONE, Journal Year: 2019, Volume and Issue: 14(2), P. e0211457 - e0211457

Published: Feb. 1, 2019

The use of Beauveria bassiana in biological control agricultural pests is mainly hampered by environmental factors, such as elevated temperatures and low humidity. These limitations, further amplified a global warming scenario, could nullify strategies based on this fungus. identification thermotolerant B. isolates represents possible strategy to overcome problem. In study, order maximize the probability isolation bassiana, soil samples infected insects were collected warm areas Syria. obtained fungal tested for different parameters (i.e., growth rate, sporulation spore germination) at growing ranging from 20°C 35°C. Among these (eight 11 samples), five with highest production germination 30°C their entomopathogenicity through vivo assays Ephestia kuehniella larvae. Insect mortality induced ranged 31% 100%. Two isolates, one Phyllognathus excavatus soil, caused 50% larval less than four days, reaching values exceeding 92% ten days. two molecularly identified sensu stricto using three markers ITS, Bloc EF1-α). Considering promising results, studies are ongoing, testing efficiency field conditions agents insect Mediterranean Subtropical regions.

Language: Английский

Citations

38
Endophytic Beauveria bassiana promotes drought tolerance and early flowering in corn DOI

Laiju Kuzhuppillymyal-Prabhakarankutty,

Patricia Tamez‐Guerra, Ricardo Gómez-Flores

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2020, Volume and Issue: 36(3)

Published: March 1, 2020

Language: Английский

Citations

37
Formulation of Trichoderma asperellum TV190 for biological control of Rhizoctonia solani on corn seedlings DOI Creative Commons
W. A. T. Herrera, Oscar Valbuena,

Domenico Pavone-Maniscalco

et al.

Egyptian Journal of Biological Pest Control, Journal Year: 2020, Volume and Issue: 30(1)

Published: April 20, 2020

Abstract Environmental conditions affect biocontrol agents in a field, being appropriate formulations an alternative to overcome this problem. Formulations based on Trichoderma asperellum TV190 were prepared by emulsified mineral or vegetable oils, which protected spores from ultraviolet radiation, showing greater viability of 37–43% (mineral) and 56–63% (vegetable) than the control (8–12%). These improved antagonism T. Rhizoctonia solani under greenhouse conditions, reducing infected corn seedlings 72% 59% (vegetable). Necrotic spot size was reduced 90.04% 87.29% A granular formulation, with degreased germ spores, R. 73% reduction plants 93% necrotic size. Both liquid able improve antagonism, suggesting that these could be included agricultural pest strategies.

Language: Английский

Citations

33