RETRACTED ARTICLE:Bone mesenchymal stem cell-derived exosomal microRNA-29b-3p prevents hypoxic-ischemic injury in rat brain by activating the PTEN-mediated Akt signaling pathway DOI Creative Commons
Kun Hou, Guichen Li,

Jinchuan Zhao

и другие.

Journal of Neuroinflammation, Год журнала: 2020, Номер 17(1)

Опубликована: Фев. 3, 2020

Abstract Background Mesenchymal stem cells (MSCs) are suspected to exert neuroprotective effects in brain injury, part through the secretion of extracellular vesicles like exosomes containing bioactive compounds. We now investigate mechanism by which bone marrow MSCs (BMSCs)-derived harboring small non-coding RNA miR-29b-3p protect against hypoxic-ischemic injury rats. Methods established a rat model middle cerebral artery occlusion (MCAO) and primary cortical neuron or microvascular endothelial cell (BMEC) models oxygen glucose deprivation (OGD). Exosomes were isolated from culture medium BMSCs. treated MCAO rats with BMSC-derived vivo, likewise OGD-treated neurons BMECs vitro. then measured apoptosis- angiogenesis-related features using TUNEL CD31 immunohistochemical staining vitro Matrigel angiogenesis assays. Results The dual luciferase reporter gene assay showed that targeted protein phosphatase tensin homolog (PTEN). was downregulated PTEN upregulated cultured neurons. promoted apoptosis decreased angiogenesis, but overexpression silencing could reverse these alterations. Furthermore, negatively regulate activate Akt signaling pathway. BMSCs-derived also exerted protective OGD samples rats, where we observed promotion angiogenesis. Conclusion exosomal ameliorates ischemic promoting suppressing neuronal apoptosis, finding may be great significance treatment injury.

Язык: Английский

Current advances in ischemic stroke research and therapies DOI Creative Commons
Derek Barthels, Hiranmoy Das

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2018, Номер 1866(4), С. 165260 - 165260

Опубликована: Сен. 16, 2018

Язык: Английский

Процитировано

538

Signaling pathways involved in ischemic stroke: molecular mechanisms and therapeutic interventions DOI Creative Commons
Chuan Qin, Sheng Yang,

Yun‐Hui Chu

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2022, Номер 7(1)

Опубликована: Июль 6, 2022

Abstract Ischemic stroke is caused primarily by an interruption in cerebral blood flow, which induces severe neural injuries, and one of the leading causes death disability worldwide. Thus, it great necessity to further detailly elucidate mechanisms ischemic find out new therapies against disease. In recent years, efforts have been made understand pathophysiology stroke, including cellular excitotoxicity, oxidative stress, cell processes, neuroinflammation. meantime, a plethora signaling pathways, either detrimental or neuroprotective, are also highly involved forementioned pathophysiology. These pathways closely intertwined form complex network. Also, these reveal therapeutic potential, as targeting could possibly serve approaches stroke. this review, we describe categorize them based on pathophysiological processes they participate in. Therapeutic associated with mentioned above, discussed. Meanwhile, clinical trials regarding potentially target involved, summarized details. Conclusively, review elucidated potential molecular related underlying summarize targeted various pathophysiology, particular reference future prospects for treating

Язык: Английский

Процитировано

461

Neuronal injuries in cerebral infarction and ischemic stroke: From mechanisms to treatment (Review) DOI Creative Commons

Yunfei Zhao,

Xiao‐Jing Zhang, Xinye Chen

и другие.

International Journal of Molecular Medicine, Год журнала: 2021, Номер 49(2)

Опубликована: Дек. 8, 2021

Stroke is the leading cause of disabilities and cognitive deficits, accounting for 5.2% all mortalities worldwide. Transient or permanent occlusion cerebral vessels leads to ischemic strokes, which constitutes majority strokes. Ischemic strokes induce brain infarcts, along with tissue death focal neuronal damage. The infarct size neurological severity after stroke episodes depends on time period since occurrence, ischemia, systemic blood pressure, vein systems location amongst others. a complex disease, injuries have been focus current studies. present review will provide basic pathological background infarcts. Moreover, major mechanisms underlying are summarized. This also briefly summarize some representative clinical trials up‑to‑date treatments that applied

Язык: Английский

Процитировано

349

Apoptosis regulation in the penumbra after ischemic stroke: expression of pro- and antiapoptotic proteins DOI
Anatoly B. Uzdensky

APOPTOSIS, Год журнала: 2019, Номер 24(9-10), С. 687 - 702

Опубликована: Июнь 29, 2019

Язык: Английский

Процитировано

253

RNA-Seq Identifies Circulating miR-125a-5p, miR-125b-5p, and miR-143-3p as Potential Biomarkers for Acute Ischemic Stroke DOI Open Access
Steffen Tiedt, Matthias Prestel, Rainer Malik

и другие.

Circulation Research, Год журнала: 2017, Номер 121(8), С. 970 - 980

Опубликована: Июль 20, 2017

Currently, there are no blood-based biomarkers with clinical utility for acute ischemic stroke (IS). MicroRNAs show promise as disease markers because of their cell type-specific expression patterns and stability in peripheral blood.To identify circulating microRNAs associated IS, determine temporal course up to 90 days post-stroke, explore an early diagnostic marker.We used RNA sequencing study changes a discovery sample 20 patients IS matched healthy control subjects. We further applied quantitative real-time polymerase chain reaction independent samples validation (40 40 controls), replication (200 100 subjects), 72 transient attacks. Sampling patient plasma was done immediately upon hospital arrival. identified, validated, replicated 3 differentially expressed microRNAs, which were upregulated compared both subjects (miR-125a-5p [1.8-fold; P=1.5×10-6], miR-125b-5p [2.5-fold; P=5.6×10-6], miR-143-3p [4.8-fold; P=7.8×10-9]) attack (miR-125a-5p: P=0.003; miR-125b-5p: miR-143-3p: P=0.005). Longitudinal analysis levels after revealed normalization starting at day 2 while miR-125a-5p remained elevated. Levels all depended on platelet numbers spike-in experiment but unaffected by chemical hypoxia Neuro2a cells experimental models. In random forest classification, miR-125a-5p, miR-125b-5p, differentiated between area under the curve 0.90 (sensitivity: 85.6%; specificity: 76.3%), superior multimodal cranial computed tomography obtained routine diagnostics 72.5%) previously reported (neuron-specific enolase: curve=0.69; interleukin 6: curve=0.82).A set (miR-125a-5p, miR-143-3p) associates might have marker.

Язык: Английский

Процитировано

233

Therapeutic targets of neuroprotection and neurorestoration in ischemic stroke: Applications for natural compounds from medicinal herbs DOI Open Access
Ting Zhu, Lei Wang, Liping Wang

и другие.

Biomedicine & Pharmacotherapy, Год журнала: 2022, Номер 148, С. 112719 - 112719

Опубликована: Фев. 12, 2022

Neuroprotective and neurorestorative therapy represent two major drug intervention strategies for ischemic stroke. Multiple factors such as excitotoxicity, inflammation, angiogenesis, neurogenesis are the main pathological processes that underlie acute chronic brain injury. Furthermore, their intimate interactions mediate blood-brain barrier permeability, increase neurovascular unit structural damage well a hemorrhagic transformation during We aimed to review current understandings of underlying mechanisms neuroprotection neurorestoration in Notably, traditional Chinese medicine (TCM) has notable advantages comprehensive treatment overall regulation multi-site multi-target diseases. Therefore, we reviewed recent advances natural compounds from medicinal herbs possess bioactivities simultaneously promoting (e.g., oxidative stress, apoptosis, autophagy) neurogenesis, axonal sprouting) following ischemia These were divided into glycosides (astragaloside IV, gastrodin, ginsenoside Rg1 salidroside), flavonoids (baicalin, icariin, puerarin breviscapine), phenols (resveratrol, curcumin salvianolic acid B), terpenes (ginkgolide B catalpol). found all exhibited anti-brain activities vivo vitro experiments by and, or neurorestoration. This tracks summarizes progress past five years explore active molecular TCMs produce pro-neuroprotection pro-neurorestoration. Additionally, provide another basis reference supporting TCMs, which could ultimately lead development precise clinical medications stroke treatment.

Язык: Английский

Процитировано

182

Microglia and monocytes in inflammatory CNS disease: integrating phenotype and function DOI Creative Commons
Alanna G. Spiteri, Claire L. Wishart, Roger Pamphlett

и другие.

Acta Neuropathologica, Год журнала: 2021, Номер 143(2), С. 179 - 224

Опубликована: Дек. 1, 2021

Abstract In neurological diseases, the actions of microglia, resident myeloid cells CNS parenchyma, may diverge from, or intersect with, those recruited monocytes to drive immune-mediated pathology. However, defining precise roles each cell type has historically been impeded by lack discriminating markers and experimental systems capable accurately identifying them. Our ability distinguish microglia from in neuroinflammation advanced with single-cell technologies, new drugs that identify deplete them, respectively. Nevertheless, focus individual studies on particular types, diseases approaches limited our connect phenotype function more widely across diverse pathologies. Here, we critically review, tabulate integrate disease-specific functions immune profiles provide a comprehensive atlas responses viral encephalitis, demyelination, neurodegeneration ischemic injury. emphasizing differential severe neuroinflammatory disease inflammatory pathways common equally incapacitating less inflammation. We examine these findings context human highlight benefits inherent limitations animal models impede facilitate clinical translation. This enables us contrasting, non-redundant often opposing could be targeted therapeutically.

Язык: Английский

Процитировано

148

Single-cell transcriptomic analysis of the immune cell landscape in the aged mouse brain after ischemic stroke DOI Creative Commons
Xuan Li,

Jingjun Lyu,

Ran Li

и другие.

Journal of Neuroinflammation, Год журнала: 2022, Номер 19(1)

Опубликована: Апрель 7, 2022

Ischemic stroke is a medical emergency that primarily affects the elderly. A complex immune response in post-stroke brain constitutes key component of pathophysiology. This study aimed to determine how cell populations aged based on molecular profiles individual cells.

Язык: Английский

Процитировано

74

Microglia-mediated neuroinflammation and neuroplasticity after stroke DOI Creative Commons
Yuan Wang, Rehana K. Leak, Guodong Cao

и другие.

Frontiers in Cellular Neuroscience, Год журнала: 2022, Номер 16

Опубликована: Авг. 16, 2022

Stroke remains a major cause of long-term disability and mortality worldwide. The immune system plays an important role in determining the condition brain following stroke. As resident innate cells central nervous system, microglia are primary responders defense network covering entire parenchyma, exert various functions depending on dynamic communications with neurons, astrocytes, other neighboring under both physiological or pathological conditions. Microglia activation polarization is crucial for damage repair ischemic stroke, considered double-edged sword neurological recovery. can exist pro-inflammatory states promote secondary damage, but they also secrete anti-inflammatory cytokines neurotrophic factors facilitate recovery In this review, we focus mechanisms microglia-mediated neuroinflammation neuroplasticity after ischemia relevant potential microglia-based interventions stroke therapy.

Язык: Английский

Процитировано

73

Neuroimmune mechanisms and therapies mediating post-ischaemic brain injury and repair DOI
Takashi Shichita, Hiroaki Ooboshi, Akihiko Yoshimura

и другие.

Nature reviews. Neuroscience, Год журнала: 2023, Номер 24(5), С. 299 - 312

Опубликована: Март 27, 2023

Язык: Английский

Процитировано

61