Transcription dynamics and genome organization in the mammalian nucleus: Recent advances
Molecular Cell,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Catching the glucocorticoid receptor in the act: lessons from fluorescence fluctuation methods
Biochemical and Biophysical Research Communications,
Год журнала:
2025,
Номер
748, С. 151327 - 151327
Опубликована: Янв. 13, 2025
Язык: Английский
The shifting paradigm of chromatin structure: from the 30-nm chromatin fiber to liquid-like organization
Proceedings of the Japan Academy Series B,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
The
organization
and
dynamics
of
chromatin
are
critical
for
genome
functions
such
as
transcription
DNA
replication/repair.
Historically,
was
assumed
to
fold
into
the
30-nm
fiber
progressively
arrange
larger
helical
structures,
described
in
textbook
model.
However,
over
past
15
years,
extensive
evidence
including
our
studies
has
dramatically
transformed
view
from
a
static,
regular
structure
one
that
is
more
variable
dynamic.
In
higher
eukaryotic
cells,
forms
condensed
yet
liquid-like
domains,
which
appear
be
basic
unit
structure,
replacing
fiber.
These
domains
maintain
proper
accessibility,
ensuring
regulation
reaction
processes.
During
mitosis,
these
assemble
form
gel-like
mitotic
chromosomes,
further
constrained
by
condensins
other
factors.
Based
on
available
evidence,
I
discuss
physical
properties
live
emphasizing
its
viscoelastic
nature-balancing
local
fluidity
with
global
stability
support
functions.
Язык: Английский
Behaviors of nucleosomes with mutant histone H4s in euchromatic domains of living human cells
Histochemistry and Cell Biology,
Год журнала:
2024,
Номер
162(1-2), С. 23 - 40
Опубликована: Май 14, 2024
Язык: Английский
Replication-dependent histone (Repli-Histo) labeling dissects the physical properties of euchromatin/heterochromatin in living human cells.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 21, 2024
Abstract
A
string
of
nucleosomes,
where
genomic
DNA
is
wrapped
around
histones,
organized
in
the
cell
as
chromatin.
Chromatin
varies
greatly,
from
euchromatin
to
heterochromatin,
its
genome
functions.
It
important
understand
how
heterochromatin
physically
different
euchromatin.
However,
their
specific
labeling
methods
living
cells
are
limited.
To
address
this,
we
have
developed
replication-dependent
histone
(Repli-Histo
labeling)
label
nucleosomes
and
based
on
replication
timing.
We
investigated
local
nucleosome
motion
four
chromatin
classes
human
mouse
cells.
found
that
more
euchromatic
regions
(earlier
replicated
regions)
show
greater
motion.
Notably,
profile
each
class
persists
throughout
interphase.
Genome
essentially
with
motions,
even
though
timing
program
perturbed.
Our
findings,
combined
computational
modeling,
suggest
earlier
accessibility
can
be
a
major
determinant
genome-wide
Язык: Английский
Rethinking chromatin accessibility: from compaction to dynamic interactions
Current Opinion in Genetics & Development,
Год журнала:
2024,
Номер
90, С. 102299 - 102299
Опубликована: Дек. 19, 2024
Язык: Английский
Seeing genomes
Histochemistry and Cell Biology,
Год журнала:
2024,
Номер
162(1-2), С. 1 - 2
Опубликована: Июнь 8, 2024
Hallmarks of glucocorticoid receptor condensates involvement in transcription regulation
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 15, 2024
Abstract
Several
proteins
necessary
for
mRNA
production
concentrate
in
intranuclear
condensates,
which
are
proposed
to
affect
transcriptional
output.
The
glucocorticoid
receptor
(GR)
is
a
ligand-activated
transcription
factor
that
regulates
the
expression
of
hundreds
genes
relevant
many
physiological
and
pathological
processes.
As
with
all
members
steroid
family,
GR
forms
condensates
unknown
function.
Here,
we
examine
whether
involved
regulation
using
Airyscan
super-resolution
microscopy
nano-antibodies
targeting
initiation
elongating
states
RNA
polymerase
II
(Pol2).
We
observed
subpopulations
colocalizing
initiating
and,
surprisingly,
Pol2
foci.
analysis
mutants
different
outputs
suggests
correlation
between
condensate
formation
capability
initiation.
Moreover,
number
molecules
within
elongation
appears
be
linked
activity.
Taken
together,
our
data
an
involvement
elongation.
Язык: Английский