Metabolites,
Год журнала:
2022,
Номер
12(1), С. 87 - 87
Опубликована: Янв. 17, 2022
Clinical
metabolomics
emerged
as
a
novel
approach
for
biomarker
discovery
with
the
translational
potential
to
guide
next-generation
therapeutics
and
precision
health
interventions.
However,
reproducibility
in
clinical
research
employing
data
is
challenging.
Checklists
are
helpful
tool
promoting
reproducible
research.
Existing
checklists
that
promote
primarily
focused
on
metadata
may
not
be
sufficient
ensure
processing.
This
paper
provides
checklist
including
actions
need
taken
by
researchers
make
computational
steps
studies.
We
developed
an
eight-item
includes
criteria
related
reusable
sharing
workflow
development.
also
provided
recommended
tools
resources
complete
each
item,
well
GitHub
project
template
process.
The
concise
easy
follow.
Studies
follow
this
use
facilitate
other
reproduce
results
easily
efficiently.
Molecular Horticulture,
Год журнала:
2022,
Номер
2(1)
Опубликована: Июль 23, 2022
Over
the
past
decade,
systems
biology
and
plant-omics
have
increasingly
become
main
stream
in
plant
research.
New
developments
mass
spectrometry
bioinformatics
tools,
methodological
schema
to
integrate
multi-omics
data
leveraged
recent
advances
proteomics
metabolomics.
These
progresses
are
driving
a
rapid
evolution
field
of
research,
greatly
facilitating
our
understanding
mechanistic
aspects
metabolisms
interactions
plants
with
their
external
environment.
Here,
we
review
MS-based
metabolomics
tools
workflows
special
focus
on
applications
research
using
several
case
studies
related
stress
response,
gene/protein
function
characterization,
metabolic
signaling
pathways
exploration,
natural
product
discovery.
We
also
present
projection
concerning
future
perspectives
development
including
challenges
for
system
biology.
This
is
intended
provide
readers
an
overview
how
advanced
MS
technology,
integrated
application
can
be
used
advance
Separations,
Год журнала:
2019,
Номер
6(4), С. 47 - 47
Опубликована: Окт. 2, 2019
Metal–organic
frameworks
(MOFs)
have
attracted
recently
considerable
attention
in
analytical
sample
preparation,
particularly
when
used
as
novel
sorbent
materials
solid-phase
microextraction
(SPME).
MOFs
are
highly
ordered
porous
crystalline
structures,
full
of
cavities.
They
formed
by
inorganic
centers
(metal
ion
atoms
or
metal
clusters)
and
organic
linkers
connected
covalent
coordination
bonds.
Depending
on
the
ratio
such
precursors
synthetic
conditions,
characteristics
resulting
MOF
vary
significantly,
thus
drifting
into
a
countless
number
interesting
with
unique
properties.
Among
astonishing
features
MOFs,
their
high
chemical
thermal
stability,
easy
tuneability,
simple
synthesis,
impressive
surface
area
(which
is
highest
known),
most
attractive
that
makes
them
outstanding
SPME.
This
review
offers
an
overview
current
state
use
different
SPME
configurations,
all
cases
covering
extraction
devices
coated
(or
incorporating)
particular
emphases
preparation.
Molecules,
Год журнала:
2020,
Номер
25(21), С. 4869 - 4869
Опубликована: Окт. 22, 2020
Solid
phase
extraction
(SPE)
is
an
analytical
procedure
developed
with
the
purpose
of
separating
a
target
analyte
from
complex
sample
matrix
prior
to
quantitative
or
qualitative
determination.
The
such
treatment
twofold:
elimination
constituents
that
could
interfere
detection
process
even
damage
equipment
as
well
enriching
in
so
it
readily
available
for
detection.
Dispersive
solid
(dSPE)
recent
development
standard
SPE
technique
attracting
growing
attention
due
its
remarkable
simplicity,
short
time
and
low
requirement
solvent
expenditure,
accompanied
by
high
effectiveness
wide
applicability.
This
review
aims
thoroughly
survey
recently
conducted
studies
focusing
on
methods
utilizing
novel,
interesting
nanomaterials
dSPE
sorbents,
known
materials
have
been
only
successfully
applied
techniques,
evaluate
their
performance
suitability
based
comparison
previously
reported
procedures.
TrAC Trends in Analytical Chemistry,
Год журнала:
2023,
Номер
166, С. 117151 - 117151
Опубликована: Июнь 24, 2023
The
exposome
is
the
measure
of
total
environmental
exposures
and
has
a
significant
impact
on
human
health
outcomes.
Exposomics
research
typically
examines
complex
biological
samples
assesses
100s
to
1,000s
small
molecules
associated
with
chemical
exposure
events.
To
acquire
robust
comprehensive
datasets
encompassing
broad
range
multi-class
biomarkers
exposures,
high-resolution
tandem
mass
spectrometry
have
emerged
as
preferred
analytical
tools
in
field.
Due
matrix
effects
interferences
together
presence
very
low
abundance
analytes,
sensitivity
reproducibility
plagued
exposomic
studies.
This
especially
true
for
large
sample
sets
where
adequate
robustness
frequently
lacking.
A
possible
solution
this
problem
development
non-discriminatory
preparation
methods
which
can
reduce
while
increasing
reproducibility.
However,
techniques
intrinsically
result
discrete
loss
analytes
leading
outstanding
challenge
finding
proper
balance.
Therefore,
approaches
decrease
preserving
coverage
need
be
developed.
In
review,
procedures
currently
applied
related
fields
are
summarized
critically
evaluated.
includes
comparison
advantages
limitations
between
different
such
solid-phase
extraction,
liquid
QuEChERS,
filters
dilute
shoot
chromatography-mass
analysis
focus.
Based
evaluation,
recommendations
promoting
balanced
strategies
future
applications
provided.
Biotechnology Advances,
Год журнала:
2024,
Номер
71, С. 108307 - 108307
Опубликована: Янв. 5, 2024
Bioassays
are
the
main
tool
to
decipher
bioactivities
from
natural
resources
thus
their
selection
and
quality
critical
for
optimal
bioprospecting.
They
used
both
in
early
stages
of
compounds
isolation/purification/identification,
later
evaluate
safety
efficacy.
In
this
review,
we
provide
a
comprehensive
overview
most
common
bioassays
discovery
development
new
bioactive
with
focus
on
marine
bioresources.
We
present
list
practical
considerations
selecting
appropriate
discuss
detail
typically
explore
antimicrobial,
antibiofilm,
cytotoxic,
antiviral,
antioxidant,
anti-ageing
potential.
The
concept
control
bioassay
validation
introduced,
followed
by
considerations,
which
advancing
higher
stage
development.
conclude
providing
an
application-oriented
view
focused
pharmaceuticals,
food
supplements,
cosmetics,
industrial
pipelines
where
currently
known
products
hold
highlight
importance
gaining
reliable
results,
as
these
serve
starting
point
application-based
further
testing,
well
consideration
regulatory
authorities.
