Frontiers in Microbiology,
Год журнала:
2019,
Номер
10
Опубликована: Авг. 23, 2019
The
Asian
citrus
psyllid
(Diaphorina
citri)
is
a
major
pest
of
trees
as
it
transmits
Candidatus
Liberibacter
asiaticus
(CLas).
composition
host's
microbiota
can
affect
the
evolution
and
ecological
distribution
host.
This
study
monitored
compositional
shifts
in
through
all
life
stages
(egg,
nymph
1–5
stages,
adult)
by
next-generation
sequencing
quantitative
real-time
PCR.
There
were
clear
differences
both
α-
β-diversity
stages.
Microbiota
diversity
was
markedly
higher
2–5
than
adult,
egg,
1
Proteobacteria
dominant
D.
citri,
representing
>
97.5%
total
bacterial
community,
Profftella
armature
genus
Data
from
qPCR
analysis
showed
an
exponential
increase
populations
three
citri
endosymbionts:
armature,
Carsonella
ruddii,
Wolbachia.
gut
bacterium
Pantoea
present
but
2-5
substantially
differed
among
egg–nymph
1,
nymphs
2–5,
adult
Therefore,
we
successfully
characterized
dynamics
thus
identified
shift
during
cycle
16S
rRNA
gene
Moreover,
suggested
that
acquired
ability
to
bear
CLas
at
stage.
enhances
our
understanding
microbial
establishment
developing
provides
reference
resource
for
identification
potential
biocontrol
approaches
against
this
pest.
ABSTRACT
Drosophila
suzukii
Matsumura
is
an
invasive
species
of
vinegar
fly
that
has
become
a
prominent
pest
berries
and
other
soft-skinned
fruits.
Unlike
most
species,
female
D.
flies
lay
their
eggs
in
ripening
ripe
fruits
larvae
develop
within
the
fruit.
To
understand
how
utilize
fruits,
which
usually
have
low
levels
protein,
we
investigated
microbiota
field-captured
laboratory-reared
further
examined
combined
influence
diet
microbes
on
host
fitness.
Field-captured
were
associated
with
diverse
microbiota,
varied
significantly
sampling
location
season.
In
contrast,
possessed
strikingly
lower
bacterial
abundance
diversity.
A
comparison
conventionally
reared
(CR)
germ-free
(GF)
revealed
does
not
alter
its
development
but
decreases
life
span
under
conditions
nutrient-sufficient
diet.
However,
essential
for
strawberry-based
or
blueberry-based
fruit
diets.
This
developmental
failure
could
be
rescued
by
reassociation
single
fungal
addition
high
quantity
heat-killed
microbes.
addition,
found
proteins
are
limiting
respect
to
fruit-based
diets
GF
show
signs
protein
starvation.
Taken
together,
our
study
results
demonstrate
provides
key
required
fresh
Our
work
shows
impact
fitness
depends
strongly
nutritional
conditions.
IMPORTANCE
Animals
commonly
specific
microbes,
play
important
roles
little
information
about
function
been
available
,
also
known
as
Spotted
wing
drosophila.
structure
affected
environment,
where
variable
depending
situation.
For
instance,
presence
deleterious
growing
protein-rich
yet
beneficial
protein-poor
Additionally,
must
feed
obtain
necessary
larval
strawberries
blueberries.
report
validates
complexity
seen
host-microbe
interactions
may
provide
useful
control.
Frontiers in Microbiology,
Год журнала:
2018,
Номер
9
Опубликована: Фев. 1, 2018
Eco-evolutionary
dynamics
of
microbiotas
at
the
macroscale
level
are
largely
driven
by
ecological
variables.
The
diet
and
living
environment
oriental
fruit
fly,
Bactrocera
dorsalis,
diversify
during
development,
providing
a
natural
system
to
explore
convergence,
divergence,
repeatability
in
patterns
microbiota
as
function
host
diet,
phylogeny,
environment.
Here,
we
characterized
47
B.
dorsalis
individuals
from
three
distinct
populations
16S
rRNA
amplicon
sequencing.
A
significant
deviation
was
found
within
larvae,
pupae,
adults
each
population.
Pupae
were
an
increased
bacterial
taxonomic
functional
diversity.
Principal
components
analysis
showed
that
clearly
separated
into
clusters.
Acetobacteraceae,
Lactobacillaceae,
Enterobacteriaceae
predominant
families
larval
adult
samples,
PICRUSt
indicated
phosphoglycerate
mutases
transketolases
significantly
enriched
while
mutases,
transketolases,
proteases
adults,
which
may
support
digestive
larvae
adults.
abundances
Intrasporangiaceae,
Dermabacteraceae
(mainly
Brachybacterium)
Brevibacteriaceae
Brevibacterium)
higher
antibiotic
transport
ATP-binding
protein
permease
pathways
there
well,
indicating
defensive
pupae.
Overall,
differences
likely
contribute
nutrient
assimilation
environments.
Journal of Insect Science,
Год журнала:
2020,
Номер
20(3)
Опубликована: Апрель 20, 2020
Abstract
After
its
initial
discovery
in
California
2008,
Drosophila
suzukii
Matsumura
has
become
one
of
the
most
important
invasive
agricultural
pest
insects
across
climate
zones
much
Asia,
Europe,
North
America,
and
South
America.
Populations
D.
have
demonstrated
notable
behavioral
physiological
plasticity,
adapting
to
diverse
environmental
climatic
conditions,
interspecific
competition,
novel
food
sources,
potential
predators.
This
adaptability
plasticity
enabled
rapid
range
expansion
diversified
niche
use
by
suzukii,
making
it
a
species
particularly
suited
changing
habitats
conditions.
article
reviews
factors
evidence
that
influence
promotes
this
species’
invasiveness.
Journal of Pest Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 1, 2025
Carpophilus
truncatus
(Murray),
a
nitidulid
beetle,
has
become
major
threat
to
almond
industries
globally.
While
there
are
existing
mass
trapping
strategies
for
other
species,
an
effective
lure
this
nut-attacking
pest
is
urgently
required.
This
study
leverages
our
knowledge
of
the
chemical
ecology
Carpophilus,
particularly
its
relationship
with
gut-associated
yeasts,
develop
new
semiochemical
attractant
C.
truncatus.
Wickerhamomyces
rabaulensis
was
identified
as
predominant
yeast
in
field-collected
Field
bioassays
demonstrated
that
traps
baited
live
cultures
W.
captured
more
beetles
compared
those
Hanseniaspora
guilliermondii,
from
stone
fruit-attacking
species.
GC–MS
analysis
revealed
both
qualitative
and
quantitative
differences
odour
profiles
two
yeasts.
Seven
volatile
compounds
were
headspace
confirmed
be
detected
by
antennae
using
GC-EAD.
Choice-test
showed
adult
preferred
synthetic
blend
based
on
volatiles
over
commercial
designed
fruit
trials
various
formulations
indicated
modified
containing
isoamyl
acetate
isobutyl
attractive
caught
fewer
non-target
species
such
hemipterus.
demonstrates
potential
insect-yeast
developing
semiochemical-based
lures
monitoring
orchards.
Journal of Pest Science,
Год журнала:
2017,
Номер
91(2), С. 651 - 660
Опубликована: Ноя. 2, 2017
Yeasts
play
an
important
role
in
nutrition
physiology
and
host
attraction
of
many
Drosophila
species,
associations
with
various
yeast
species
are
documented
for
several
drosophilid
flies.
The
pest
suzukii
(Matsumura)
has
a
predominant
association
the
Hanseniaspora
uvarum.
However,
research
not
been
conducted
on
nutritional
yeasts
associated
D.
(spotted
wing
drosophila).
Therefore,
this
study,
we
determined
whether
dietary
was
nutritionally
relevant
closely
positively
affected
life-history
traits.
Our
results
confirm
crucial
larval
development
survival
suzukii.
Furthermore,
found
specific
effects
H.
uvarum
Candida
sp.
survival.
Observations
egg-laying
behaviour
cherry
fruits
artificially
colonised
different
revealed
that
number
eggs
laid
increased
Saccharomyces
cerevisiae.
Journal of Economic Entomology,
Год журнала:
2017,
Номер
111(1), С. 283 - 292
Опубликована: Сен. 22, 2017
This
research
aimed
to
more
clearly
describe
the
interactions
of
Drosophila
suzukii
(Matsumura;
Diptera:
Drosophilidae)
with
microorganisms
that
may
contribute
spoilage
or
quality
loss
wine
grapes
during
harvest.
Experiments
were
conducted
in
controlled
laboratory
experiments
and
under
field
conditions
determine
these
effects.
Laboratory
trials
determined
role
insect
contact
oviposition
vector
bacteria
onto
grapes.
In
field,
roles
key
organoleptic
parameters
grape
fruit
ripening
assessed
their
relative
contribution
potential
as
ripened.
Finally,
relationships
egg
larval
infestation
sour
rot
levels.
Non-ovipositional
indicated
elevated
levels
microbiota
when
D.
was
present.
exponentially
increased
concentration
acetic
acid
bacteria.
Both
incised
sound
berries
showed
a
significant
increase
concentrations
exposed
suzukii.
Volatile
acidity
higher
treatments
infested
Fruit
only
eggs
did
not
develop
volatile
acidity.
Larva-infested
9.5%
samples
developed
after
14
d.
Sound
less
susceptible
development
associated
spoilage.
risk
vectored
by
adults.
Acetic
induced
fermentation
produced
several
compounds
contributing
Spoilage
create
positive
feedback
loop
attracts
both
other
drosophilids,
which
additional
Drosophila
suzukii
differs
from
other
melanogaster
group
members
in
their
proclivity
for
laying
eggs
fresh
fruit
rather
than
fermenting
fruits.
Olfaction
and
gustation
play
a
critical
role
during
insect
niche
formation,
these
senses
are
largely
mediated
by
two
important
receptor
families:
olfactory
gustatory
receptors
(Ors
Grs).
Earlier
work
our
laboratory
has
revealed
how
the
landscape
of
D.
is
dominated
volatiles
derived
its
unique
niche.
Signaling
reception
evolve
synchrony,
since
interaction
ligands
together
mediate
chemosensory
behavior.
Here,
we
manually
annotated
Ors
Grs
close
relatives,
biarmipes
takahashii,
compared
repertoires
to
those
drosophilids
identify
candidate
chemoreceptors
associated
with
suzukii's
unusual
utilization.
Our
comprehensive
annotations
genomes
three
species,
comparative
analysis
provide
insights
into
evolution
chemosensation
pestiferous
suzukii.
We
total
71
Or
genes
suzukii,
nine
being
pseudogenes
(12.7
%).
Alternative
splicing
brings
62
encoding
66
Ors.
Duplications
Or23a
Or67a
expanded
repertoire,
while
pseudogenization
Or74a,
Or85a,
Or98b
reduced
number
functional
roughly
same
as
species
group.
Seventy-one
intact
Gr
were
81.
identified
signatures
positive
selection
at
nodes
leading
copies
largest
lineage,
Or67a,
also
showed
signs
external
nodes.
chemoreceptor
context
drosophilids,
including
closest
relatives
(D.
takahashii),
several
adaptation
ecological
