UPLC‐HRMS‐based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long‐term drought stress

Plant Cell & Environment, Год журнала: 2018, Номер 42(1), С. 115 - 132

Опубликована: Март 15, 2018

Abstract Genetic improvement for drought tolerance in chickpea requires a solid understanding of biochemical processes involved with different physiological mechanisms. The objective this study is to demonstrate genetic variations altered metabolic levels varieties (tolerant and sensitive) grown under contrasting water regimes through ultrahigh‐performance liquid chromatography/high‐resolution mass spectrometry‐based untargeted metabolomic profiling. Chickpea plants were exposed stress at the 3‐leaf stage 25 days, leaves harvested 14 days after imposition stress. Stress produced significant reduction chlorophyll content, F v / m , relative shoot root dry weight. Twenty known metabolites identified as most important by 2 methods including analysis partial least squares discriminant analysis. pronounced increase accumulation due was demonstrated allantoin, l ‐proline, ‐arginine, ‐histidine, ‐isoleucine, tryptophan. Metabolites that showed decreased level conditions choline, phenylalanine, gamma‐aminobutyric acid, alanine, tyrosine, glucosamine, guanine, aspartic acid. Aminoacyl‐tRNA plant secondary metabolite biosynthesis amino acid metabolism or synthesis pathways producing variation conditions. Metabolic changes light highlighted pools affect adjustment reduced impacts.

Язык: Английский

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Evolution of Multiple Sensory Systems Drives Novel Egg-Laying Behavior in the Fruit Pest Drosophila suzukii

Current Biology, Год журнала: 2017, Номер 27(6), С. 847 - 853

Опубликована: Март 1, 2017

The rise of a pest species represents unique opportunity to address how evolve new behaviors and adapt novel ecological niches [1]. We this question by studying the egg-laying behavior Drosophila suzukii, an invasive agricultural that has spread from Southeast Asia Europe North America in last decade [2]. While most closely related lay their eggs on decaying plant substrates, D. suzukii oviposits ripening fruit, thereby causing substantial economic losses fruit industry [3-8]. evolved enlarged, serrated ovipositor presumably plays key role enabling females pierce skin ripe [9]. Here, we explore selects oviposition sites, differs species. have combined behavioral experiments multiple with neurogenetics mutant analysis show specific preference for fruit. Our results also establish changes mechanosensation, olfaction, gustation contributed shift. observations support model which emergence as is consequence progressive modification several sensory systems, collectively underlie radical change behavior.

Язык: Английский

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Advances in the Chemical Ecology of the Spotted Wing Drosophila (Drosophila suzukii) and its Applications

Journal of Chemical Ecology, Год журнала: 2018, Номер 44(10), С. 922 - 939

Опубликована: Июль 27, 2018

Язык: Английский

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Insect olfactory communication in a complex and changing world

Current Opinion in Insect Science, Год журнала: 2020, Номер 42, С. 1 - 7

Опубликована: Май 6, 2020

Язык: Английский

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Plasticity Is Key to Success of Drosophila suzukii (Diptera: Drosophilidae) Invasion

Journal of Insect Science, Год журнала: 2020, Номер 20(3)

Опубликована: Апрель 20, 2020

Abstract After its initial discovery in California 2008, Drosophila suzukii Matsumura has become one of the most important invasive agricultural pest insects across climate zones much Asia, Europe, North America, and South America. Populations D. have demonstrated notable behavioral physiological plasticity, adapting to diverse environmental climatic conditions, interspecific competition, novel food sources, potential predators. This adaptability plasticity enabled rapid range expansion diversified niche use by suzukii, making it a species particularly suited changing habitats conditions. article reviews factors evidence that influence promotes this species’ invasiveness.

Язык: Английский

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Enormous expansion of the chemosensory gene repertoire in the omnivorous German cockroach Blattella germanica

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Год журнала: 2018, Номер 330(5), С. 265 - 278

Опубликована: Март 22, 2018

The acquisition of genome sequences from a wide range insects and other arthropods has revealed broad positive correlation between the complexity their chemical ecology size chemosensory gene repertoire. German cockroach Blattella germanica is an extreme omnivore largest repertoire known for arthropod, exceeding even highly polyphagous spider mite Tetranychus urticae. While Odorant Receptor family not particularly large, with 123 genes potentially encoding 134 receptors (105 intact), Gustatory greatly expanded to 431 545 (483 second only mite. Ionotropic olfactory gustatory vastly at least 897 (604 in arthropods, far surpassing 150 dampwood termite Zootermopsis nevadensis. Commensurately, Binding Protein 109 (all intact). Comparison more specialized, but phylogenetically related termite, within Dictyoptera, reveals considerable losses massive species-specific expansions cockroach. lost function 11%-41% these three chemoreceptor families pseudogenization, most are young events, implying rapid turnover along major expansions, presumably response changes its ecology.

Язык: Английский

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Evolution of Reproductive Behavior

Genetics, Год журнала: 2020, Номер 214(1), С. 49 - 73

Опубликована: Янв. 1, 2020

Abstract Behaviors associated with reproduction are major contributors to the evolutionary success of organisms and subject many forces, including natural sexual selection, conflict. Successful involves a range behaviors, from finding an appropriate mate, courting, copulation, successful production (in oviparous animals) deposition eggs following mating. As consequence, behaviors genes often under strong selection evolve rapidly. Courtship rituals in flies follow multimodal pattern, mediated through visual, chemical, tactile, auditory signals. Premating allow males females assess species identity, reproductive state, condition their partners. Conflicts between “interests” individual males, and/or strategies females, drive evolution behaviors. For example, seminal proteins transmitted by show evidence rapid evolution, positive selection. Postmating oviposition sites, highly variable Drosophila span spectrum generalists obligate specialists. Chemical recognition features prominently adaptation host plants for feeding oviposition. Selection acting on variation pre-, peri-, postmating can lead isolation incipient speciation. Response at genetic level include expansion gene families, such as those detecting pheromonal cues mating, or changes expression leading visual wing spots that assessed during Here, we consider behavior two distinct, yet complementary, scales. Some studies take microevolutionary approach, identifying networks involved reproduction, then dissecting genetics underlying complex D. melanogaster. Other macroevolutionary comparing across genus how these might correlate environmental cues. A full synthesis this field will require unification levels.

Язык: Английский

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Gene Expression and Diet Breadth in Plant-Feeding Insects: Summarizing Trends

Trends in Ecology & Evolution, Год журнала: 2019, Номер 35(3), С. 259 - 277

Опубликована: Дек. 2, 2019

Язык: Английский

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Behavioral and Genomic Sensory Adaptations Underlying the Pest Activity ofDrosophila suzukii

Molecular Biology and Evolution, Год журнала: 2021, Номер 38(6), С. 2532 - 2546

Опубликована: Фев. 11, 2021

Abstract Studying how novel phenotypes originate and evolve is fundamental to the field of evolutionary biology as it allows us understand organismal diversity generated maintained. However, determining basis challenging involves orchestrated changes at multiple biological levels. Here, we aim overcome this challenge by using a comparative species framework combining behavioral, gene expression, genomic analyses egg-laying substrate-choice behavior invasive pest Drosophila suzukii. First, used behavioral assays evolution ripe fruit oviposition preference in D. suzukii compared with closely related subpulchrella biarmipes well melanogaster. We show that lay eggs on both rotten fruits, suggesting transition was gradual. Second, two-choice assays, studied suzukii, subpulchrella, biarmipes, melanogaster differentially process key sensory cues distinguishing from during egg-laying. found suzukii’s for part mediated through species-specific stiff substrates. Last, sequenced annotated high-quality genome subpulchrella. Using approaches, identified candidate genes involved ability seek out target fruits. Our results provide detail stepwise activity indicating important when finding host, molecular mechanisms potentially underlying their adaptation new ecological niche.

Язык: Английский

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Linking anthocyanin diversity, hue, and genetics in purple corn

G3 Genes Genomes Genetics, Год журнала: 2021, Номер 11(2)

Опубликована: Янв. 11, 2021

While maize with anthocyanin-rich pericarp (purple corn) is rising in popularity as a source of natural colorant for foods and beverages, information on color range stability-factors associated anthocyanin decorations compositional profiles-is currently limited. Furthermore, to maximize the scalability meet growing demands, both concentrations agronomic performance must improve purple corn varieties. Using diversity present landrace, Apache Red, we generated population variable flavonoid profiles-flavanol-anthocyanin condensed forms (0-83%), acylated anthocyanins (2-72%), pelargonidin-derived (5-99%), C-glycosyl flavone co-pigments up 1904 µg/g, content 1598 µg/g. Each aspect profiles was found play role either resulting extract hue or intensity. With genotyping-by-sequencing this population, mapped aspects profile. Major quantitative trait loci (QTLs) type were near previously identified only aleurone-pigmented varieties [Purple aleurone1 (Pr1) Anthocyanin acyltransferase1 (Aat1)]. A QTL P1 (Pericarp color1) flavanol-anthocyanin forms. significant peonidin-derived candidate S-adenosylmethionine-dependent methyltransferase also identified, warranting further investigation. Mapping total produced signals Aat1, aleurone-associated bHLH R1 (Colored1), plant color-associated MYB, Pl1 (Purple plant1), recessive intensifier, In1 (Intensifier1), several unidentified candidates. This represents one most diverse pericarp-pigmented characterized date. Moreover, candidates here will serve branching points future research studying genetic molecular processes determining profile pericarp.

Язык: Английский

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