Ovario- protective effect of Moringa oleifera leaf extract against cyclophosphamide-induced oxidative ovarian damage and reproductive dysfunction in female rats

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 7, 2025

Abstract It is crucial to develop new tactics prevent ovarian tissue damage in women whose reproductive toxicity caused by chemotherapy. The present investigation was performed assess the protective effects of Moringa oleifera (M. oleifera) leaf extract on cyclophosphamide (CP)-induced and dysfunction. Thirty-two female, healthy Wistar albino rats were randomly assigned four groups (8 rats/group). first group given saline intraperitoneally (i.p.). second a single dose CP (200 mg/kg; i.p.). third fourth M. (150 250 orally) for 20 days before receiving final day experiment. Hormonal assessments, including follicle stimulating hormone (FSH), luteinizing (LH), estrogen (ES), 24 h after administration. In addition, antioxidant status inflammatory response against evaluated. Moreover, detailed histopathological ultra- structural observations conducted. For evaluation statistical significance between different groups; One-way analysis variance (ANOVA) with Tukey’s post hoc test adopted. Our findings revealed that subjected showed increased levels FSH, LH, malondialdehyde (MDA), tumor necrosis factor-alpha, interleukin-8 decreased ES glutathione. Pre-treatment (250 statistically significant (p values < 0.05) as it could improve hormonal changes, oxidative stress indices, pro- mediator levels. Consequently, marked improvement observed uterine architectures, normal reserve endothelium tubular glands. conclusion, mg/kg) be used pharmaceutical supplement because protects female CP-induced

Язык: Английский

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Tangeretin alleviates sepsis-induced acute lung injury by inhibiting ferroptosis of macrophage via Nrf2 signaling pathway

Chinese Medicine, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 15, 2025

Abstract Background Sepsis-induced acute lung injury (ALI) is a severe clinical condition accompanied with high mortality. Tangeretin, which widely found in citrus fruits, has been reported to exert antioxidant and anti-inflammatory properties. However, whether tangeretin protects against sepsis-induced ALI the potential mechanisms remain unclear. Methods We established an model via intraperitoneally injected 5 mg/kg lipopolysaccharides (LPS) for 12 h. Tangeretin was applied 30 min before LPS treatment. Dexamethasone (Dex) used as positive control. Hematoxylin eosin (HE) staining protein content bronchoalveolar lavage fluid (BALF) were determined detect degree of injury. RNA-seq also explore effect on ALI. In vitro, RAW264.7 treated Nrf2 siRNA, expression ferroptosis-associated biomarkers, including glutathione peroxidase 4 (GPX4) prostaglandin-endoperoxide synthase 2 (PTGS2) assessed. Glutathione (GSH), malondialdehyde (MDA) levels, reactive oxygen species (ROS) inflammatory factors both vivo vitro. Furthermore, mice inhibitor (ML385) verify mechanism inhibiting ferroptosis. Data analyzed using one way analysis variance or two-tailed unpaired t tests. Results Our study demonstrated that significantly alleviated injury, reversed LPS-induced reduction GPX4 GSH, mitigates elevation PTGS2 MDA levels. reduced 4-HNE iron Besides, levels LPS-stimulated IL-6, IL-1β TNF-α decreased by tangeretin. bioinformatics inhibited response. Mechanistically, we identified GPX4-dependent lipid peroxidation through activation Nrf2. The silence abolished inhibitory oxidative stress, response ferroptosis cells. Additionally, all protective effects inhibitor-treated mice. Conclusion critical contributing promising therapeutic candidate, effectively upregulating signaling pathway.

Язык: Английский

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The roles of dietary polyphenols at crosstalk between type 2 diabetes and Alzheimer's disease in ameliorating oxidative stress and mitochondrial dysfunction via PI3K/Akt signaling pathways

Ageing Research Reviews, Год журнала: 2024, Номер 99, С. 102416 - 102416

Опубликована: Июль 13, 2024

Язык: Английский

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An Update on the Potential of Tangeretin in the Management of Neuroinflammation-Mediated Neurodegenerative Disorders

Life, Год журнала: 2024, Номер 14(4), С. 504 - 504

Опубликована: Апрель 14, 2024

Neuroinflammation is the major cause of neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease. Currently available drugs present relatively low efficacy are not capable modifying course disease or delaying its progression. Identifying well-tolerated brain-penetrant agents plant origin could fulfil pressing need for novel treatment techniques neuroinflammation. Attention has been drawn to a large family flavonoids in citrus fruits, which may function strong nutraceuticals slowing down development progression This review aimed at elucidating summarizing effects flavonoid tangeretin (TAN) management neuroinflammation-mediated disorders. A literature survey was performed using various resources, including ScienceDirect, PubMed, Google Scholar, Springer, Web Science. The data revealed that TAN exhibited immense neuroprotective addition anti-oxidant, anti-diabetic, peroxisome proliferator-activated receptor-γ agonistic effects. mainly mediated through inhibition oxidative inflammatory pathways via regulating multiple signaling pathways, c-Jun N-terminal kinase, phosphoinositide 3-kinase, mitogen-activated protein nuclear factor erythroid-2-related 2, extracellular-signal-regulated CRE-dependent transcription. In conclusion, potential prevent neuronal death by neuroinflammatory can be developed an auxiliary therapeutic agent

Язык: Английский

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Tangeretin offers neuroprotection against colchicine-induced memory impairment in Wistar rats by modulating the antioxidant milieu, inflammatory mediators and oxidative stress in the brain tissue

BMC Complementary Medicine and Therapies, Год журнала: 2025, Номер 25(1)

Опубликована: Фев. 4, 2025

Язык: Английский

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Tangeretin attenuates bleomycin-induced pulmonary fibrosis by inhibiting epithelial-mesenchymal transition via the PI3K/Akt pathway

Frontiers in Pharmacology, Год журнала: 2023, Номер 14

Опубликована: Сен. 15, 2023

Background: Pulmonary fibrosis (PF) is a terminal pathological change in variety of lung diseases characterized by excessive deposition extracellular matrix, for which effective treatment lacking. Tangeretin (Tan), flavonoid derived from citrus, has been shown to have wide range pharmacological effects. This study aimed investigate the role and potential mechanisms Tan on pulmonary fibrosis. Methods: A model was established administering bleomycin through tracheal drip, followed or pirfenidone gavage. HE Masson staining were employed assess extent Subsequently, Western blot, enzyme-linked immunosorbent assay (ELISA), RNA sequencing, immunohistochemistry techniques uncover protective mechanism PF mice. Furthermore, A549 cells stimulated with TGF-β1 induce epithelial-mesenchymal transition (EMT) demonstrate effectiveness mitigating PF. Results: significantly ameliorated bleomycin-induced fibrosis, improved fibrotic changes, collagen lungs, reduced inflammation oxidative stress. The KEGG pathway enrichment analysis revealed higher number enriched genes PI3K/Akt pathway. Additionally, can inhibit EMT process related Conclusion: Taken together, above research results indicate that suppresses inflammation, stress, BLM-induced via agent

Язык: Английский

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Evidence for chromium crosses blood brain barrier from the hypothalamus in chromium mice model

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 273, С. 116179 - 116179

Опубликована: Март 1, 2024

It has been shown that exposure to hexavalent Chromium, Cr (Ⅵ), via nasal cavity can have neurotoxicological effects and induces behavioral impairment due the fact blood brain barrier (BBB) does not cover olfactory bulb. But whether (Ⅵ) cross BBB a toxicological in central nervous system (CNS) remains unclear. Therefore, we investigated of on mice treated with different concentrations time (14 days 28 days) intraperitoneal injection. Results revealed accumulated hypothalamus (HY) timely dependent manner. Much more severer neuropathologies was observed group exposed for than 14 days. Gliosis, neuronal morphological abnormalities, synaptic degeneration, disruption number loss were HY. In terms mechanism, Nrf2 related antioxidant stress signaling dysfunction activated NF-κB inflammatory pathway HY intoxication mice. And these defects appeared Taking together, proved enter weaker is most vulnerable CNS region exposure. The concentration increased along time. cause disruption, degeneration gliosis through pathway. This finding improves our understanding neurological dysfunctions individuals who occupational provided potential therapeutic targets treat pathologies induced by (Ⅵ).

Язык: Английский

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Employing a Toxic Aging Coin approach to assess hexavalent chromium (Cr[VI])-induced neurotoxic effects on behavior: Heads for age differences

Toxicology and Applied Pharmacology, Год журнала: 2024, Номер 489, С. 117007 - 117007

Опубликована: Июнь 18, 2024

Язык: Английский

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Effect of tangeretin on cisplatin‐induced oxido‐inflammatory brain damage in rats

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(14)

Опубликована: Июль 1, 2024

Cisplatin (CIS) is a platinum-derived chemotherapeutic agent commonly utilized in the treatment of various malignant tumours. However, anticancer doses drug cause serious damage to brain. This study aimed determine potential protective effects tangeretin, which has antioxidant and anti-inflammatory properties, cisplatin-induced neurotoxicity on BALB/c mice brains. Male were randomized separated into four groups. Tangeretin was given for 10 days by gavage. CIS injected as single dose mg/kg intraperitoneally (ip) 10th day. Brain tissues, malondialdehyde (MDA), total glutathione (tGSH), peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) nitric oxide (NO) levels measured oxidative myeloperoxidase, tumour necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), IL-6 IL-10 inflammatory activity. In addition, 8-OHdG caspase-3 analysed immunofluorescence methods. While administration remarkably elevated reactive oxygen species, MDA, NO brain tissue compared control, tGSH, GPx, SOD CAT significantly decreased. Also, it been detected that TNF-α, IL-1β obtained CIS-treated groups increased well decreased, thereby elevating response. immunoreactivity neurons with administration. Treatment tangeretin ameliorated deterioration oxidant/antioxidant status, overpowered neuroinflammation neurotoxicity-induced apoptosis. shows beneficial CIS-induced neurodegeneration. Possible mechanisms underlying these include properties tangeretin.

Язык: Английский

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Tangeretin alleviates myocardial ischemia-reperfusion injury by inhibiting ferroptosis via targeting nicotinamide phosphoribosyltransferase (NAMPT)

European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177568 - 177568

Опубликована: Апрель 1, 2025

Язык: Английский

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