International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(9), С. 4692 - 4692
Опубликована: Апрель 25, 2024
In
drug
discovery,
selecting
targeted
molecules
is
crucial
as
the
target
could
directly
affect
efficacy
and
treatment
outcomes.
As
a
member
of
CCN
family,
CTGF
(also
known
CCN2)
an
essential
regulator
in
progression
various
diseases,
including
fibrosis,
cancer,
neurological
disorders,
eye
diseases.
Understanding
regulatory
mechanisms
different
diseases
may
contribute
to
discovery
novel
candidates.
Summarizing
CTGF-targeting
-inhibitory
drugs
also
beneficial
for
analysis
efficacy,
applications,
limitations
these
disease
models.
Therefore,
we
reviewed
structure,
development
order
provide
more
references
future
discovery.
Molecules,
Год журнала:
2023,
Номер
28(14), С. 5587 - 5587
Опубликована: Июль 22, 2023
Gastric
cancer
is
one
of
the
most
frequent
types
neoplasms
worldwide,
usually
presenting
as
aggressive
and
difficult-to-manage
tumors.
The
search
for
new
structures
with
anticancer
potential
encompasses
a
vast
research
field
in
which
natural
products
arise
promising
alternatives.
In
this
scenario,
piperine,
an
alkaloid
Piper
species,
has
received
attention
due
to
its
biological
activity,
including
attributes.
present
work
proposes
three
heating-independent,
reliable,
low-cost,
selective
methods
obtaining
piperine
from
nigrum
L.
(Black
pepper).
Electronic
(SEM)
optical
microscopies,
X-ray
diffraction,
nuclear
magnetic
resonance
spectroscopies
(13C
1H
NMR),
(UV–Vis,
photoluminescence,
FTIR)
confirm
obtention
crystals.
MTT
assay
reveals
that
samples
exhibit
good
cytotoxic
activity
against
primary
metastasis
models
gastric
cell
lines
Brazilian
Amazon.
showed
cytotoxicity
on
evaluated
models,
revealing
higher
effectiveness
cells
bearing
degree
aggressiveness.
Moreover,
investigated
crystals
demonstrated
ability
act
enhancer
when
combined
traditional
chemotherapeutics
(5-FU
GEM),
allowing
drugs
achieve
same
effect
employing
lower
concentrations.
These
results
establish
molecule
therapy
investigations
cancer,
both
isolated
form
or
bioenhancer.
Bioprospecting
is
the
investigation
of
biodiversity
to
search
new
resources
commercial
value.
The
bioprospecting
program
includes
scientific
and
economic
activities
that
explore
genes,
organisms,
species
in
diverse
habitats
ecosystem
while
safeguarding
conservation,
traditional
knowledge,
growth
local
communities.
natural
products
obtained
from
have
contributed
immensely
medicinal
agricultural
fields
recently
bioremediation,
biomimetic
engineering,
aquaculture,
nanotechnology
fields.
These
include
pharmaceuticals,
industrial
chemicals,
metabolic
pathways,
behaviors,
materials.
They
are
used
as
such
product
development
or
may
be
physical
blueprints
designs
leads.
Since
involves
worldwide
activities,
ethical
legal
issues
also
addressed
prevent
biopiracy
other
malpractices.
In
this
chapter,
we
will
discuss
about
bioprospecting,
rational
processes,
their
drawbacks,
approaches
advance
utility
agricultural,
medical,
Journal of Functional Biomaterials,
Год журнала:
2022,
Номер
13(4), С. 255 - 255
Опубликована: Ноя. 19, 2022
The
antibacterial
properties
of
nanomaterials
(NMs)
can
be
exploited
in
a
range
consumer
products
(e.g.,
wound
dressings,
food
packaging,
textiles,
medicines).
There
is
also
interest
the
exploitation
NMs
as
treatments
for
infectious
diseases
to
help
combat
antibiotic
resistance.
Whilst
activity
has
been
assessed
vitro
and
vivo
numerous
studies,
methodology
used
very
varied.
Indeed,
while
approaches
are
available
assess
effect
vitro,
they
have
not
yet
systematically
their
suitability
sensitivity
testing
NMs.
It
therefore
timely
consider
what
assays
should
prioritised
screen
majority
existing
studies
focused
on
investigating
effects
exhibited
by
silver
(Ag)
employed
limited
assays.
We
compared
copper
oxide
(CuO)
Escherichia
coli,
Pseudomonas
aeruginosa,
Staphylococcus
aureus,
Bacillus
subtilis
at
various
concentrations
(12.5–200
µg/mL)
using
battery
tests
(well
disc
diffusion,
plate
counts—time-kill
method,
optical
density
measurement—OD,
Alamar
Blue
live/dead
viability
assays,
quantitative
polymerase
chain
reaction).
CuO
were
most
toxic
B.
E.
P.
aeruginosa
was
least
sensitive
strain.
All
detected
NMs;
however,
varied
sensitivity,
time,
cost,
technical
difficulty
requirement
specialized
equipment.
In
future,
we
suggest
that
combination
provide
robust
assessment
particular,
recommend
time-kill
OD
due
greater
sensitivity.
standard
operating
protocols
developed
so
harmonised
approach.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(3), С. 380 - 380
Опубликована: Март 16, 2024
Empirically,
in
Indonesia,
the
leaves
of
Cassia
alata
L.
(candle
bush
or
ketepeng
cina)
have
been
used
as
a
topical
antifungal
agent.
Malassezia
furfur
is
natural
microorganism
found
human
body.
It
among
factors
contributing
to
conditions
such
pityriasis
versicolor,
common,
benign,
superficial
fungal
infection
skin
that
closely
associated
with
seborrheic
dermatitis
and
dandruff.
This
study
aimed
explore
C.
leaves,
starting
from
determining
activity
against
M.
identification
major
compounds
ethyl
acetate
n-hexane
fractions,
then
we
carried
out
molecular
docking
fraction
lanosterol
14-alpha
demethylase.
The
method
was
disc
diffusion
technique
test
activity,
LC-MS/MS
for
compound
identification,
homology
modeling
through
Swiss
Models
docking.
fractions
extract
showed
concentration-dependent
furfur.
LCMS/MS
analysis
revealed
five
fractions.
demonstrated
highest
binding
affinity
stearidonic
acid
at
−7.2
kcal/mol.
can
be
concluded
furfur,
supported
by
both
vitro
silico
studies.
Angewandte Chemie International Edition,
Год журнала:
2021,
Номер
60(14), С. 7893 - 7899
Опубликована: Янв. 15, 2021
Abstract
Proof‐of‐concept
is
provided
that
a
large
estate
of
16‐membered
macrolide
antibiotics
can
be
reached
by
“unified”
approach.
The
key
building
block
was
formed
on
scale
an
asymmetric
vinylogous
Mukaiyama
aldol
reaction;
its
alkene
terminus
then
converted
either
into
the
corresponding
methyl
ketone
Wacker
oxidation
or
chain‐extended
aldehyde
catalyst‐controlled
branch‐selective
hydroformylation.
These
transformations
ultimately
opened
access
to
two
structurally
distinct
series
targets.
Notable
late‐stage
maneuvers
comprise
rare
example
ruthenium‐catalyzed
redox
isomerization
1,3‐enyne‐5‐ol
1,3‐diene‐5‐one
derivative,
as
well
elaboration
tertiary
propargylic
alcohol
acyloin
trans
‐hydrostannation/Chan‐Lam‐type
coupling.
Moreover,
this
case
study
illustrates
underutilized
possibility
forging
complex
macrolactone
rings
transesterification
under
essentially
neutral
conditions.
Sustainable Chemistry and Pharmacy,
Год журнала:
2022,
Номер
28, С. 100739 - 100739
Опубликована: Июнь 11, 2022
This
study
investigated
the
applications
of
a
myrtle
extract
obtained
by
supercritical
fluid
extraction
(SFE),
technique
considered
environmentally
friendly,
as
possible
antimicrobial
ingredient
in
cosmetic
and
pharmaceutical
formulations.
The
was
at
230
bar
45
°C,
for
2
h,
with
flow
CO2
0.3
kg
h−1.
A
cosolvent
(ethanol)
also
used,
0.09
extracts
thus
were
tested
against
seven
Gram-positive
bacteria
one
yeast
using
well
diffusion
broth
dilution
techniques.
results
showed
that
exhibits
good
antibacterial
activity
all
strains
studied
is
superior
to
most
those
conventional
methods.
Antifungal
present
but
lesser
extent.
Cell
viability
studies
carried
out
exposing
HaCat
cells
range
concentrations,
from
0.1
μg/mL
up
60
24
MTT
assay.
Salmonella
mutagenicity
assay
applied
evaluate
antimutagenicity
extract.
suggest
green
solvent,
CO2,
safe
could
reduce
genotoxic
damage
induced
reactive
oxygen
species
(ROSs).
Journal of Medical Virology,
Год журнала:
2022,
Номер
94(6), С. 2438 - 2452
Опубликована: Фев. 9, 2022
The
ongoing
COVID-19
pandemic
severely
impacts
global
public
health
and
economies.
To
facilitate
research
on
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
virology
antiviral
discovery,
a
noninfectious
viral
replicon
system
operating
under
biosafety
level
containment
is
warranted.
We
report
herein
the
construction
characterization
of
two
SARS-CoV-2
minigenome
systems.
First,
we
constructed
IVT-CoV2-Rep
complementary
DNA
template
to
generate
messenger
RNA
(mRNA)
with
nanoluciferase
(NLuc)
reporter
via
in
vitro
transcription
(IVT).
mRNA
transfection
assay
demonstrated
rapid
transient
replication
variety
cell
lines,
which
could
be
completely
abolished
by
known
inhibitors.
Our
data
also
suggest
that
phenotype
not
due
host
innate
responses.
In
addition,
have
developed
DNA-launched
BAC-CoV2-Rep,
supports
in-cell
as
initial
template.
BAC-CoV2-Rep
exhibited
much
stronger
longer
signal
compared
version.
found
portion
NLuc
was
derived
from
spliced
resistant
treatment,
especially
during
early
phase
after
transfection.
summary,
established
systems
are
suitable
for
basic
research,
hold
promise
stable
line
development
further
optimization.
Journal of Materials Chemistry B,
Год журнала:
2022,
Номер
10(42), С. 8664 - 8671
Опубликована: Янв. 1, 2022
Bacterial
wound
infection
has
brought
a
serious
threat
to
human
health
and
caused
huge
economic
losses.
Attempts
develop
biomaterials
with
excellent
antibacterial
effects
are
meaningful
promote
healing.
Herein,
we
report
multifunctional
nanoreactor
nutrient
consumption
reactive
oxygen
species
(ROS)
generation
capabilities
for
skin
repair.
The
was
constructed
by
the
encapsulation
of
glucose
oxidase
(GOx)
into
Cu2+-doped
zeolite-based
imidazole
framework
(ZIF-8)
through
one-pot
synthesis
method.
not
only
consumes
nutrients
bacteria
GOx-driven
oxidation
reaction,
but
also
generates
highly
toxic
hydroxyl
radicals
(˙OH)
kill
via
Cu+-mediated
Fenton-like
reaction.
Moreover,
Zn2+
released
from
is
capable
exhibiting
synergistic
activity.
In
addition
mediating
reactions,
Cu2+
promotes
angiogenesis
accelerate
Thus,
ability
cut
off
supply
starve
bacteria,
produce
ROS
healing,
enabling
it
be
promising
treatment
infection.
Pharmaceuticals,
Год журнала:
2022,
Номер
15(10), С. 1302 - 1302
Опубликована: Окт. 21, 2022
The
increase
in
antibiotic
resistance
poses
a
major
threat
to
global
health.
Actinomycetes,
the
Gram-positive
bacteria
of
order
Actinomycetales,
are
fertile
producers
bioactive
secondary
metabolites,
including
antibiotics.
Nearly
two-thirds
antibiotics
that
used
for
treatment
bacterial
infections
were
originally
isolated
from
actinomycetes
strains
belonging
genus
Streptomyces.
This
emphasizes
importance
discovery.
However,
identification
new
antimicrobial
compound
and
exploration
its
mode
action
very
challenging
tasks.
Therefore,
different
approaches
enable
“detection”
an
characterization
mechanisms
leading
biological
activity
indispensable.
Beyond
bioinformatics
tools
facilitating
biosynthetic
gene
clusters
(BGCs),
whole
cell-screenings—in
which
cells
exposed
actinomycete-derived
compounds—are
common
strategy
applied
at
early
stage
drug
development.
More
recently,
target-based
have
been
established.
In
this
case,
candidates
tested
interactions
with
usually
validated
targets.
review
focuses
on
bioactivity-based
screening
methods
provides
readers
overview
most
relevant
assays
investigation
action.
Moreover,
article
includes
examples
successful
application
these
suggestions
improvement.