Comparison of In Vitro Approaches to Assess the Antibacterial Effects of Nanomaterials

Journal of Functional Biomaterials, Journal Year: 2022, Volume and Issue: 13(4), P. 255 - 255

Published: Nov. 19, 2022

The antibacterial properties of nanomaterials (NMs) can be exploited in a range consumer products (e.g., wound dressings, food packaging, textiles, medicines). There is also interest the exploitation NMs as treatments for infectious diseases to help combat antibiotic resistance. Whilst activity has been assessed vitro and vivo numerous studies, methodology used very varied. Indeed, while approaches are available assess effect vitro, they have not yet systematically their suitability sensitivity testing NMs. It therefore timely consider what assays should prioritised screen majority existing studies focused on investigating effects exhibited by silver (Ag) employed limited assays. We compared copper oxide (CuO) Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis at various concentrations (12.5–200 µg/mL) using battery tests (well disc diffusion, plate counts—time-kill method, optical density measurement—OD, Alamar Blue live/dead viability assays, quantitative polymerase chain reaction). CuO were most toxic B. E. P. aeruginosa was least sensitive strain. All detected NMs; however, varied sensitivity, time, cost, technical difficulty requirement specialized equipment. In future, we suggest that combination provide robust assessment particular, recommend time-kill OD due greater sensitivity. standard operating protocols developed so harmonised approach.

Language: Английский

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Potential of supercritical fluid myrtle extracts as an active ingredient and co-preservative for cosmetic and topical pharmaceutical applications

Sustainable Chemistry and Pharmacy, Journal Year: 2022, Volume and Issue: 28, P. 100739 - 100739

Published: June 11, 2022

This study investigated the applications of a myrtle extract obtained by supercritical fluid extraction (SFE), technique considered environmentally friendly, as possible antimicrobial ingredient in cosmetic and pharmaceutical formulations. The was at 230 bar 45 °C, for 2 h, with flow CO2 0.3 kg h−1. A cosolvent (ethanol) also used, 0.09 extracts thus were tested against seven Gram-positive bacteria one yeast using well diffusion broth dilution techniques. results showed that exhibits good antibacterial activity all strains studied is superior to most those conventional methods. Antifungal present but lesser extent. Cell viability studies carried out exposing HaCat cells range concentrations, from 0.1 μg/mL up 60 24 MTT assay. Salmonella mutagenicity assay applied evaluate antimutagenicity extract. suggest green solvent, CO2, safe could reduce genotoxic damage induced reactive oxygen species (ROSs).

Language: Английский

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Discovery of furopyridine-based compounds as novel inhibitors of Janus kinase 2: In silico and in vitro studies

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129308 - 129308

Published: Jan. 11, 2024

Language: Английский

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Novel Antibacterial Agents and Emerging Therapies in the Fight Against Multidrug-Resistant Acinetobacter baumannii

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: 200, P. 107361 - 107361

Published: Jan. 31, 2025

Language: Английский

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Inhalable pH-responsive core-shell nanocarriers with PEGylated chitosan/alginate layer-by-layer coating for sequential drug release in lung cancer therapy

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141851 - 141851

Published: March 1, 2025

Language: Английский

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Construction and characterization of two SARS‐CoV‐2 minigenome replicon systems

Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 94(6), P. 2438 - 2452

Published: Feb. 9, 2022

The ongoing COVID-19 pandemic severely impacts global public health and economies. To facilitate research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virology antiviral discovery, a noninfectious viral replicon system operating under biosafety level containment is warranted. We report herein the construction characterization of two SARS-CoV-2 minigenome systems. First, we constructed IVT-CoV2-Rep complementary DNA template to generate messenger RNA (mRNA) with nanoluciferase (NLuc) reporter via in vitro transcription (IVT). mRNA transfection assay demonstrated rapid transient replication variety cell lines, which could be completely abolished by known inhibitors. Our data also suggest that phenotype not due host innate responses. In addition, have developed DNA-launched BAC-CoV2-Rep, supports in-cell as initial template. BAC-CoV2-Rep exhibited much stronger longer signal compared version. found portion NLuc was derived from spliced resistant treatment, especially during early phase after transfection. summary, established systems are suitable for basic research, hold promise stable line development further optimization.

Language: Английский

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Multifunctional nanoreactors with nutrient consumption and ROS generation capabilities for antibacterial and skin repair

Journal of Materials Chemistry B, Journal Year: 2022, Volume and Issue: 10(42), P. 8664 - 8671

Published: Jan. 1, 2022

Bacterial wound infection has brought a serious threat to human health and caused huge economic losses. Attempts develop biomaterials with excellent antibacterial effects are meaningful promote healing. Herein, we report multifunctional nanoreactor nutrient consumption reactive oxygen species (ROS) generation capabilities for skin repair. The was constructed by the encapsulation of glucose oxidase (GOx) into Cu2+-doped zeolite-based imidazole framework (ZIF-8) through one-pot synthesis method. not only consumes nutrients bacteria GOx-driven oxidation reaction, but also generates highly toxic hydroxyl radicals (˙OH) kill via Cu+-mediated Fenton-like reaction. Moreover, Zn2+ released from is capable exhibiting synergistic activity. In addition mediating reactions, Cu2+ promotes angiogenesis accelerate Thus, ability cut off supply starve bacteria, produce ROS healing, enabling it be promising treatment infection.

Language: Английский

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Antibiofilm and Immune-Modulatory Activity of Cannabidiol and Cannabigerol in Oral Environments—In Vitro Study

Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 342 - 342

Published: April 9, 2024

To evaluate the in vitro antimicrobial and antibiofilm properties immune modulatory activity of cannabidiol (CBD) cannabigerol (CBG) on oral bacteria periodontal ligament fibroblasts (PLF).

Language: Английский

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Efavirenz Repurposing Challenges: A Novel Nanomicelle-Based Antiviral Therapy Against Mosquito-Borne Flaviviruses

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 241 - 241

Published: Feb. 12, 2025

Background/Objective: World Health Organization latest statistics state that 17% of infectious diseases are transmitted by vectors, causing more than 700,000 deaths each year. Particularly, dengue (DENV), Zika (ZIKV) and yellow fever (YFV) viral infections have generated international awareness due to their epidemic proportion risks spread. In this framework, the repositioning strategy Efavirenz (EFV) represents a key clinical feature improve different antiviral therapies. Therefore, development Soluplus®-based nanomicelles (NMs) loaded with EFV (10 mg/mL) for optimized oral pharmacotherapy against ZIKV, DENV YFV was investigated. Methods: EFV-NMs were obtained an acetone diffusion technique. Micellar size in vitro micellar interaction mucin assessed dynamic light scattering. cytocompatibility investigated A549 Vero cells activity evaluated. vivo bioavailability histological studies Wistar rats. Results: encapsulation within Soluplus® NMs increased drug's apparent aqueous solubility up 4803-fold unimodal distribution ~90 nm at 25 37 °C. also pH range 1.2-7.5 its storage physicochemical stability 4 °C confirmed over 2 years. assays dispersions resulted safe nanoformulations. Interestingly, EFV-loaded exhibited significantly higher compared solution all tested flaviviruses. addition, selectivity index (SI) values reveal considerably biological efficacy cell lines infection (SI > 10). Further, drug pharmacokinetics parameters enhanced after administration NMs, being biocompatible not damage gastrointestinal segments. Conclusions: Overall, our nanoformulation highlighted potential as novel delivery platform therapy.

Language: Английский

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Evaluating the ionic liquids, commercial solvents, and pressure-swing for efficient azeotropic separation

Chemical Product and Process Modeling, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Abstract Techniques like extractive distillation and pressure swing (PSD) have been commercially developed to separate azeotropic mixtures. Extractive using conventional solvents offers advantages in achieving high purity levels, but challenges include difficulty solvent recovery, increased energy consumption, higher costs, potential negative environmental impact. Selecting appropriate is a challenging task with compromises. PSD manipulates overcome points without an entrainer, offering solvent-free operation. Ionic liquids may provide alternative distillation. Five imidazolium, ammonium pyridinium based ILs chosen investigate their impact on the separation of selected The three techniques are employed for seven different (model) mixtures evaluate commercial feasibility each process by comparing requirements Total Annual Cost (TAC). Aspen Plus software has perform comparison simulation among processes. This benchmark analysis can aid evaluating efficacy possible optimum separation. There no one-size-fits-all technique that provides best cost-effective energy-efficient every

Language: Английский

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