Science China Life Sciences, Год журнала: 2022, Номер 66(5), С. 1151 - 1169
Опубликована: Ноя. 23, 2022
Язык: Английский
Nature Ecology & Evolution, Год журнала: 2022, Номер 6(9), С. 1354 - 1366
Опубликована: Июль 11, 2022
Abstract Triploids are rare in nature because of difficulties meiotic and gametogenic processes, especially vertebrates. The Carassius complex cyprinid teleosts contains sexual tetraploid crucian carp/goldfish ( C. auratus ) unisexual hexaploid gibel carp/Prussian carp gibelio lineages, providing a valuable model for studying the evolution maintenance mechanism polyploids Here we sequence genomes two species assemble their haplotypes, which contain subgenomes (A B), to chromosome level. Sequencing coverage analysis reveals that is an amphitriploid (AAABBB) with triploid sets chromosomes; each set derived from different ancestor. Resequencing data strains show reproduction has been maintained over 0.82 million years. Comparative genomics intensive expansion alterations cell cycle-related genes oocyte-specific histone variant. Cytological assays indicate produces unreduced oocytes by alternative ameiotic pathway; however, sporadic homologous recombination high rate gene conversion also exist . These genomic changes might have facilitated purging deleterious mutations maintaining genome stability this fish. Overall, current results provide novel insights into evolutionary mechanisms reproductive success polyploid
Язык: Английский
Процитировано
53
Reviews in Aquaculture, Год журнала: 2023, Номер 15(4), С. 1501 - 1521
Опубликована: Янв. 23, 2023
Abstract Genomic resources are increasingly being used to improve the production efficiency and profitability of aquaculture. Crustaceans a large group invertebrates that encompasses some most important farmed species in aquaculture industry. However, very few crustacean genomes have been published although an genome project was proposed as early 1997. Breakthroughs next‐generation third‐generation sequencing technologies development high‐complexity sequence assembly strategies promoted publication increasing number genomes, thus paving broad way for biological genetic studies applications In this review, we summarize recent advances genomic research June 2022, including assembly, characteristics, mechanisms underlying various phenotypes, such environmental adaptation, lifestyle, development, sex determination. This review also discusses application aquaculture, dissection economic traits genome‐based selective breeding via genome‐wide association selection. High‐quality not only understanding basis biologically economically but improvement species. utilization genomics still lags far behind those other animals. Therefore, additional multi‐omics technological needed accelerate
Язык: Английский
Процитировано
27
Water Biology and Security, Год журнала: 2024, Номер 3(3), С. 100271 - 100271
Опубликована: Май 16, 2024
Aquaculture originates from ancient Chinese wisdom, and is considered the most efficient food-producing industry. About 70 years ago, New China proposed an industrial approach to fish production made a policy decision begin modern aquaculture development. As one of lucky persons since early 1980s, I have committed my scholarly career studies on genetics breeding biotechnology polyploid gibel carp (Carassius gibelio). Here, several significant recent innovations, such as proposition amphitriploid concept, association ploidy changes with reproduction transition, finding unique ameio-fusiongenesis mode, establishment controllable fertility approach, creation intermuscular bone-free mutants, well recreation desirable goldfish, are introduced emphasized active participant these innovations. Additionally, some innovations contributions also highlighted in other aspects including feed development, disease control, facilities equipment upgrading, paradigm mode changes. This comprehensive synthesis wisdom seeks provide valuable insights into field historical basis.
Язык: Английский
Процитировано
12
PLoS Genetics, Год журнала: 2024, Номер 20(3), С. e1011210 - e1011210
Опубликована: Март 27, 2024
Sex is determined by multiple factors derived from somatic and germ cells in vertebrates. We have identified amhy , dmrt1 gsdf as male foxl2 foxl3 cyp19a1a female sex determination pathway genes Nile tilapia. However, the relationship among these largely unclear. Here, we found that gonads of ; double mutants developed ovaries or underdeveloped testes with no irrespective their genetic sex. In addition, cyp19a1b triple still ovaries. The testes, while eventually contrast, spermatogenesis via up-regulation both cells. but due to . Taking respective ovary testis reported previously into consideration, demonstrated once mutated, gonad could not be rescued functional mutating any gene. reversal caused mutation other than including its upstream downstream Overall, our data suggested only gene tested indispensable for development
Язык: Английский
Процитировано
11
Science China Life Sciences, Год журнала: 2022, Номер 65(12), С. 2341 - 2353
Опубликована: Ноя. 10, 2022
Язык: Английский
Процитировано
28
Frontiers in Immunology, Год журнала: 2023, Номер 14
Опубликована: Фев. 6, 2023
It is generally considered that stress causes decreased immune function and render fish vulnerable to infection diseases. However, the molecular mechanisms between responses susceptibility infections, especially viral diseases, in remain unknown. Understanding monitoring biological consequences underlying may contribute improvement of animal welfare production efficiency. In this study, long-term exposure a variety stressors, including chasing, overcrowding, restraint stress, air mimicking chronic stresses, aquaculture practices was conducted Carassius gibel investigate on inflammation antiviral capability. With continuation stimulation, experimental gradually became insensitive net chasing feeding with accompaniment upregulated gene expressed HPI axis elevated levels hormones. As expected, stress-induced hyperglycaemia decrease insulin signaling pathway altered expression glycolysis gluconeogenesis, suggesting disturbance glycometabolism. Importantly, link intestinal homoeostasis systemic low-grade stressed C. observed, implying crosstalk among brain, intestine, other organs. Furthermore, compromised capability impaired innate immunity confirmed by RNA sequencing Cyprinid herpesvirus 2 (CyHV-2), promoting understanding enhanced fish.
Язык: Английский
Процитировано
20
Science China Life Sciences, Год журнала: 2023, Номер 66(9), С. 1976 - 1993
Опубликована: Июль 28, 2023
Язык: Английский
Процитировано
20
Aquaculture and Fisheries, Год журнала: 2024, Номер unknown
Опубликована: Июль 1, 2024
Aquaculture, a critical sector for global food security, faces the challenge of meeting growing demand while protecting wild fish populations. Gene editing, powerful genetic tool, emerges as potential solution. By modifying key genes in fish, it is expected to rapidly enhance growth rate, disease resistance, and other economically important traits, leading increased profitability, sustainability, competitiveness industry. This review navigates complex landscape gene editing aquaculture, exploring successes, challenges, prospects improved productivity, resilience. We provide guidance researchers stakeholders identifying responsible addressing economic, environmental, regulatory, ethical considerations. roadmap paves way future where empowers aquaculture meet needs upholding environmental responsibility.
Язык: Английский
Процитировано
7
Molecular Biology and Evolution, Год журнала: 2024, Номер 41(8)
Опубликована: Авг. 1, 2024
Abstract Sex chromosomes display remarkable diversity and variability among vertebrates. Compared with research on the X/Y Z/W chromosomes, which have long evolutionary histories in mammals birds, studies sex at early stages are limited. Here, we precisely assembled genomes of homozygous XX female YY male Lanzhou catfish (Silurus lanzhouensis) derived from an artificial gynogenetic family a self-fertilized family, respectively. Chromosome 24 (Chr24) was identified as chromosome based resequencing data. Comparative analysis X Y showed approximate 320 kb Y-specific region duplicate anti-Mullerian hormone type II receptor (amhr2y), is consistent findings 2 other Silurus species but different (Chr24 meridionalis Chr5 asotus). Deficiency amhr2y resulted male-to-female reversal, indicating that plays male-determining role S. lanzhouensis. Phylogenetic comparative genomics revealed common sex-determining gene initially translocated to Chr24 ancestor along expansion transposable elements. maintained lanzhouensis, whereas transition triggered turnover asotus. Additionally, duplication, translocation, degeneration were observed regions species. These present clear case for trajectory including origin, repeat sequence expansion, gathering region, turnover.
Язык: Английский
Процитировано
6
National Science Review, Год журнала: 2022, Номер 10(2)
Опубликована: Окт. 28, 2022
ABSTRACT Assembly of a complete Y chromosome is significant challenge in animals with an XX/XY sex-determination system. Recently, we created YY-supermale yellow catfish by crossing XY males sex-reversed females, providing valuable model for Y-chromosome assembly and evolution. Here, assembled highly homomorphic X chromosomes sequencing genomes the YY supermale XX female catfish, revealing their nucleotide divergences only less than 1% same gene compositions. The sex-determining region (SDR) was identified to locate within physical distance 0.3 Mb FST scanning. Strikingly, incipient sex were revealed originate via autosome–autosome fusion characterized rearranged SDR downstream site. We found that at very early stage differentiation, as no clear evidence evolutionary strata classical structure features recombination suppression rather late evolution observed. Significantly, number sex-antagonistic mutations accumulation repetitive elements discovered SDR, which might be main driver initial establishment between young chromosomes. Moreover, distinct three-dimensional chromatin organizations supermales exhibited denser chromosome, while they respectively have significantly spatial interactions female- male-related genes compared other autosomes. configuration well nucleus organization neomale remodeled after reversal similar those supermales, male-specific loop containing open region. Our results elucidate origin remodeling sexual plasticity.
Язык: Английский
Процитировано
27