Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review
Plants,
Год журнала:
2023,
Номер
12(5), С. 1136 - 1136
Опубликована: Март 2, 2023
Orchids,
with
their
astonishingly
stunning
flowers,
dominate
the
international
floricultural
market.
They
are
considered
prized
assets
for
commercial
applications
in
pharmaceutical
and
industries
as
they
possess
high
therapeutic
properties
superior
ornamental
values.
The
alarming
depletion
of
orchid
resources
due
to
excessive
unregulated
collection
mass
habitat
destruction
makes
conservation
measures
an
extreme
priority.
Conventional
propagation
methods
cannot
produce
adequate
number
orchids,
which
would
meet
requirement
these
plants
conservational
purposes.
In
vitro
using
semi-solid
media
offers
outstanding
prospect
rapidly
producing
quality
on
a
large
scale.
However,
(SS)
system
has
shortcomings
low
multiplication
rates
production
costs.
Orchid
micropropagation
temporary
immersion
(TIS)
overcomes
limitations
SS
by
reducing
costs
making
scaleup
full
automation
possible
plant
production.
current
review
highlights
different
aspects
TIS
benefits
drawbacks
rapid
generation.
Язык: Английский
Development of robust in vitro propagation protocol and cyto-genetic fidelity assessment of Siraitia grosvenorii (monk fruit)
Scientia Horticulturae,
Год журнала:
2024,
Номер
331, С. 113142 - 113142
Опубликована: Март 29, 2024
Язык: Английский
In vitro flower induction and cyto-genetic fidelity assessment of Chlorophytum comosum (Thunb.) Jacques var. comosum
South African Journal of Botany,
Год журнала:
2023,
Номер
159, С. 678 - 685
Опубликована: Июль 12, 2023
Язык: Английский
In vitro seed germination and protocorm development of Eulophia graminea Lindl. (Orchidaceae).
Опубликована: Авг. 30, 2024
Abstract
Effects
of
various
factors
on
seed
germination
and
subsequent
protocorm
growth
Eulophia
graminea,
an
endangered
terrestrial
orchid
species
native
to
Japan,
were
examined
using
New
Dogashima
medium
without
applying
plant
regulators
natural
ingredients.
For
the
culture,
aseptic
mature
seeds
collected
from
undehiscent
pods
used
throughout
study,
supplemented
with
29.2
mM
sucrose
8
g/L
agar
was
as
standard
medium.
examining
effect
calcium
hypochlorite
germination,
pre-treated
for
0–60
min
solution
containing
1%
available
chlorine
cultured
The
effects
other
pretreatment.
culture
temperature
at
range
15–35°C.
Optimum
concentration
suitable
kind
carbohydrate
0-175.3
sucrose,
maltose,
trehalose
glucose,
respectively.
As
results,
rate
increased
increase
in
treatment
duration
maximum
85.8%
60
treatment.
concentrations
29.2–58.4
mM,
which
gave
low
rates
(7–8%).
Among
four
kinds
carbohydrates
tested,
highest
(26.8%).
On
temperatures,
both
shoot
development
obtained
30°C.
Direct
forming
rhizome
shown
this
high
temperature.
Язык: Английский
Genetic Stability in Micropropagated Orchids: Assessment by Molecular Markers and Flow Cytometry
BENTHAM SCIENCE PUBLISHERS eBooks,
Год журнала:
2023,
Номер
unknown, С. 180 - 231
Опубликована: Сен. 11, 2023
Orchids
occupy
a
significant
position
in
the
international
floricultural
market
because
of
their
spectacularly
beautiful
flowers
with
varied
sizes,
forms,
patterns,
and
colorations.
Apart
from
high
ornamental
values,
they
are
known
for
therapeutic
application
traditional
medicinal
system.
However,
natural
orchid
resources
quickly
depleting
excessive
unregulated
commercial
collection
mass
habitat
destruction.
Orchid
production
through
conventional
propagation
methods
cannot
meet
present
demands
these
plants.
Micropropagation
orchids
plant
tissue
culture
provides
an
excellent
opportunity
to
propagate
true-to-type
quality
plants
on
large
scale
rapidly.
somaclonal
variation
may
appear
vitro
clones
producing
undesired
phenotypic
molecular
defects.
It
is
obligatory
test
genetic
integrity
propagated
ensure
identical
orchids.
Genetic
stable
produced
by
evaluating
fidelity
regenerants
using
markers.
The
chapter
highlights
stability
assessment
several
micropropagated
markers
flow
cytometry
method.
Язык: Английский