Decellularized Extracellular Matrix for Remodeling Bioengineering Organoid's Microenvironment

Small, Год журнала: 2023, Номер 19(25)

Опубликована: Март 16, 2023

Abstract Over the past decade, stem cell‐ and tumor‐derived organoids are most promising models in developmental biology disease modeling, respectively. The matrix is one of three main elements construction an organoid important module its extracellular microenvironment. However, source currently available commercial matrix, Matrigel, limits application clinical medicine. It worth investigating whether original decellularized (dECM) can be exploited as improving very important. In this review, tissue decellularization protocols characteristics methods, mechanical support biological cues extraccellular (ECM), methods for multifunctional dECM responsive hydrogel, potential applications functional summarized. addition, some expectations provided applications.

Язык: Английский

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Macrophage plasticity: signaling pathways, tissue repair, and regeneration

MedComm, Год журнала: 2024, Номер 5(8)

Опубликована: Авг. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Язык: Английский

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Progress in biomaterials inspired by the extracellular matrix

Giant, Год журнала: 2024, Номер 19, С. 100323 - 100323

Опубликована: Июль 10, 2024

Inspired by the extracellular matrix (ECM), biomaterials have emerged as promising strategies in biomedical research and engineering domain, offering unique characteristics for tissue regeneration, drug delivery, therapeutic interventions, cellular investigations. The ECM, a dynamic network structure secreted various cells, primarily comprises diverse proteins capable of facilitating tissue-ECM signaling regulatory functions through its rich array bioactive substances multi-level structural properties. Drawing inspiration from intricate biochemical composition natural researchers developed to encapsulate these features create biomimetic microenvironments, such electrospinning, hydrogels/hydrogel microspheres, decellularized ECM(dECM), ECM-mimicking peptides. Furthermore, mimicking ECM components, ECM-inspired exhibit varying degrees functionalization, including providing support, cell adhesion, signal transduction, mitigating immune responses, remodeling. In summary, advancements offer significant promise addressing key challenges fields engineering, regenerative medicine, delivery.

Язык: Английский

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Functional acellular matrix for tissue repair

Materials Today Bio, Год журнала: 2022, Номер 18, С. 100530 - 100530

Опубликована: Дек. 28, 2022

In view of their low immunogenicity, biomimetic internal environment, tissue- and organ-like physicochemical properties, functionalization potential, decellularized extracellular matrix (dECM) materials attract considerable attention are widely used in tissue engineering. This review describes the composition matrices role stem-cell differentiation, discusses advantages disadvantages existing decellularization techniques, presents methods for characterization scaffolds. addition, we discuss progress use dECMs cartilage, skin, nerve, muscle repair transplantation or regeneration different whole organs (e.g., kidneys, liver, uterus, lungs, heart), summarize shortcomings using organ after refunctionalization, examine corresponding future prospects. Thus, present helps to further systematize application functionalized tissue/organ keep researchers up date on recent dECM usage.

Язык: Английский

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Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(21), С. 13040 - 13040

Опубликована: Окт. 27, 2022

Cardiovascular diseases are the leading cause of global mortality. Over past two decades, researchers have tried to provide novel solutions for end-stage heart failure address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Cardiac decellularized extracellular matrix (dECM) has been widely explored a promising approach in tissue-regenerative medicine because its remarkable similarity original tissue. Optimized decellularization protocols combining physical, chemical, enzymatic agents developed obtain perfect balance between cell removal, ECM composition, function maintenance. However, proper assessment tissue composition is still needed before clinical translation. Recellularizing acellular scaffold with organ-specific cells evaluating extent cardiomyocyte repopulation also challenging. This review aims discuss existing literature on scaffolds, especially advantages methods preparation, pointing out areas improvement. Finally, an overview state research regarding application dECM future challenges bioengineering human suitable provided.

Язык: Английский

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Decellularized kidney extracellular matrix-based hydrogels for renal tissue engineering

Acta Biomaterialia, Год журнала: 2024, Номер 180, С. 295 - 307

Опубликована: Апрель 18, 2024

Kidney regeneration is hindered by the limited pool of intrinsic reparative cells. Advanced therapies targeting renal have potential to alleviate clinical and financial burdens associated with kidney disease. Delivery systems for cells, extracellular vesicles, or growth factors aimed at enhancing can benefit from vehicles enabling targeted delivery controlled release. Hydrogels, optimized carry biological cargo while promoting regeneration, emerged as promising candidates this purpose. This aims develop a hydrogel decellularized matrix (DKECM) explore its biocompatibility biomaterial regeneration. The resulting crosslinks temperature exhibits high concentration matrix. decellularization process efficiently removes detergent residues, yielding pathogen-free that non-hemolytic devoid α-gal epitope. Upon interaction macrophages, induces differentiation into both pro-inflammatory anti-inflammatory phenotypes, suggesting an adequate balance promote functionality in vivo. Renal progenitor cells encapsulated DKECM demonstrate higher viability proliferation than commercial collagen-I hydrogels, also expressing tubular podocyte markers long-term culture. Overall, injectable derived porcine anticipated elicit minimal host reaction fostering cell bioactivity, offering avenue settings. quest improve treatments disease crucial, given challenges faced patients on dialysis waiting transplants. Exciting new combining biomaterials revolutionize repair. In study, researchers created pig kidney. gel could be used deliver other substances help Despite coming pigs, it's safe use humans, no harmful reduced risk immune reactions. Importantly, it promotes balanced healing response body. research not only advances our knowledge repair but offers hope more effective diseases.

Язык: Английский

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Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering

Materials, Год журнала: 2023, Номер 16(8), С. 3197 - 3197

Опубликована: Апрель 18, 2023

In recent years, three-dimensional (3D) bioprinting has been widely utilized as a novel manufacturing technique by more and researchers to construct various tissue substitutes with complex architectures geometries. Different biomaterials, including natural synthetic materials, have manufactured into bioinks for regeneration using 3D bioprinting. Among the biomaterials derived from tissues or organs, decellularized extracellular matrix (dECM) internal structure variety of bioactive factors that provide mechanistic, biophysical, biochemical signals remodeling. developing dECM bioink construction substitutes. Compared other bioinks, ECM components in dECM-based can regulate cellular functions, modulate process, adjust Therefore, we conducted this review discuss current status perspectives on engineering. addition, techniques decellularization methods were also discussed study.

Язык: Английский

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Injectable decellularized extracellular matrix hydrogel promotes salivary gland regeneration via endogenous stem cell recruitment and suppression of fibrogenesis

Acta Biomaterialia, Год журнала: 2023, Номер 169, С. 256 - 272

Опубликована: Авг. 8, 2023

Язык: Английский

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Three Birds, One Stone: An Osteo‐Microenvironment Stage‐Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo‐Immunomodulation, Middle Neovascularization, and Later Osteogenesis

Advanced Science, Год журнала: 2023, Номер 11(6)

Опубликована: Дек. 7, 2023

Abstract In order to repair critical‐sized bone defects, various polylactic acid‐glycolic acid (PLGA)‐based hybrid scaffolds are successfully developed as substitutes. However, the byproducts of these PLGA‐based known acidify implanted site, inducing tiresome acidic inflammation. Moreover, degradation productions cannot offer an osteo‐friendly microenvironment at matching natural healing. Herein, inspired by atlas bone‐healing process, osteo‐microenvironment stage‐regulative scaffold (P80/D10/M10) is fabricated incorporating self‐developed decellularized matrix microparticles (DBM‐MPs) and multifunctional magnesium hydroxide nanoparticles (MH‐NPs) into PLGA with optimized proportion using low‐temperature rapid prototyping (LT‐RP) 3D‐printing technology. The cell experiments show that this P80/D10/M10 exhibits excellent properties in mechanics, biocompatibility, biodegradability, meanwhile superior stimulations osteo‐immunomodulation, angiogenesis, osteogenesis. Additionally, animal determined can a stage‐matched pattern for enhanced regeneration, namely, optimization early inflammation, middle neovascularization, later formation. Furthermore, transcriptomic analysis suggested vivo performance on defect mostly attributed regulating artery development, remodeling. Overall, study reveals provides promising treatment repair.

Язык: Английский

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Mechanism and application of fibrous proteins in diabetic wound healing: a literature review

Frontiers in Endocrinology, Год журнала: 2024, Номер 15

Опубликована: Июль 26, 2024

Diabetic wounds are more complex than normal chronic because of factors such as hypoxia, reduced local angiogenesis, and prolonged inflammation phase. Fibrous proteins, including collagen, fibrin, laminin, fibronectin, elastin etc., possess excellent inherent properties that make them highly advantageous in the area wound healing. Accumulating evidence suggests they contribute to healing process diabetic by facilitating repair remodel extracellular matrix, stimulating development vascular granulation tissue, so on. However, there is currently a lack comprehensive review application these proteins diabetes wounds. An overview fibrous protein characteristics alterations linked given this article's initial section. Next summary advanced applications last five years, acellular dermal hydrogel, foam, scaffold, electrospun nanofibrous membrane. These dressings have ability actively promote addition just covering compared traditional like gauze or bandage. Research on their role may result novel therapeutic modalities lower incidence thereby enhance health patients.

Язык: Английский

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