Gels,
Год журнала:
2024,
Номер
10(11), С. 704 - 704
Опубликована: Окт. 31, 2024
Due
to
their
indisputable
biocompatibility
and
abundant
source,
biopolymers
are
widely
used
prepare
hydrogels
for
skin
tissue
engineering.
Among
them,
cellulose
is
a
great
option
this
challenging
application
due
its
increased
water
retention
capacity,
mechanical
strength,
versatility
unlimited
availability.
Since
algae
an
unexploited
source
of
cellulose,
the
novelty
study
decellularization
two
different
species,
freshly
collected
from
Black
Sea
coast,
using
chemical
surfactants
(sodium
dodecyl
sulphate
Triton
X-100),
characterisation
resulted
complex
biopolymeric
3D
matrices.
The
nature
agent
significantly
influenced
matrices
porosity,
while
values
obtained
hydration
degree
included
them
in
hydrogel
class.
Moreover,
capacity
retain
then
controllably
release
anti-inflammatory
drug,
ibuprofen,
led
us
recommend
structures
as
drug
delivery
systems.
decellularized
macroalgae
bioadhesive
cytocompatible
direct
contact
with
human
keratinocytes
represent
support
cells.
Finally,
it
was
noticed
that
(HaCaT
cell
line)
adhered
populated
during
monitoring
period
14
days.
Heliyon,
Год журнала:
2024,
Номер
10(3), С. e24593 - e24593
Опубликована: Янв. 17, 2024
3D
bioprinting
has
unlocked
new
possibilities
for
generating
complex
and
functional
tissues
organs.
However,
one
of
the
greatest
challenges
lies
in
selecting
appropriate
seed
cells
constructing
fully
artificial
Currently,
there
are
no
cell
sources
available
that
can
fulfill
all
requirements
technologies,
each
source
possesses
unique
characteristics
suitable
specific
applications.
In
this
review,
we
explore
impact
different
technologies
bioink
materials
on
cells,
providing
a
comprehensive
overview
current
landscape
have
been
used
or
hold
potential
bioprinting.
We
also
summarized
key
points
to
guide
selection
Moreover,
offer
insights
into
prospects
bioprinted
organs,
highlighting
their
revolutionize
fields
tissue
engineering
regenerative
medicine.
Engineered Regeneration,
Год журнала:
2023,
Номер
4(4), С. 357 - 374
Опубликована: Май 16, 2023
The
skin
is
an
important
organ
of
the
human
body
that
resists
external
threats
but
lacks
sufficient
self-regeneration
ability
when
severe
damage
occurs.
However,
most
available
substitutes
cannot
achieve
ideal
restoration
complex
structures
and
multiple
functions
native
tissues.
Fortunately,
advent
decellularized
extracellular
matrix
(dECM)
offers
a
promising
approach
to
overcome
these
obstacles.
dECM,
derived
from
natural
(ECM),
possesses
similar
structure
composition,
which
constructs
environment
favorable
for
cell
performance
in
regeneration.
Moreover,
dECM
retains
good
bioactivity,
low
immunogenicity,
high
availability,
making
it
suitable
biomaterial
repair
In
this
review,
various
decellularization
methods
subsequent
evaluations
are
introduced
first,
main
sources
then
presented.
Furthermore,
recent
progress
dECM-based
biomaterials
applied
regeneration
future
perspectives
summarized.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(13), С. 15879 - 15892
Опубликована: Март 26, 2024
Tendon
regeneration
is
greatly
influenced
by
the
oxidant
and
inflammatory
microenvironment.
Persistent
inflammation
during
tendon
repair
can
cause
matrix
degradation,
adhesion,
excessive
accumulation
of
reactive
oxygen
species
(ROS),
while
ROS
affect
extracellular
remodeling
integration.
Herein,
we
used
tannic
acid
(TA)
to
modify
a
decellularized
slice
(DTS)
fabricate
functional
scaffold
(DTS-TA)
with
antioxidant
anti-inflammatory
properties
for
repair.
The
characterizations
cytocompatibility
scaffolds
were
examined
in
vitro.
activities
evaluated
vitro
further
studied
vivo
using
subcutaneous
implantation
model.
It
was
found
that
modified
DTS
combined
TA
via
hydrogen
bonds
covalent
bonds,
hydrophilicity,
thermal
stability,
biodegradability,
mechanical
characteristics
significantly
improved.
Afterward,
results
demonstrated
DTS-TA
could
effectively
reduce
increasing
M2/M1
macrophage
ratio
interleukin-4
(IL-4)
expression,
decreasing
secretion
interleukin-6
(IL-6)
interleukin-1β
(IL-1β),
as
well
scavenging
vivo.
In
summary,
provides
potential
versatile
regeneration.
Pharmaceuticals,
Год журнала:
2023,
Номер
16(5), С. 701 - 701
Опубликована: Май 5, 2023
Burns
are
a
widespread
global
public
health
traumatic
injury
affecting
many
people
worldwide.
Non-fatal
burn
injuries
leading
cause
of
morbidity,
resulting
in
prolonged
hospitalization,
disfigurement,
and
disability,
often
with
stigma
rejection.
The
treatment
burns
is
aimed
at
controlling
pain,
removing
dead
tissue,
preventing
infection,
reducing
scarring
risk,
tissue
regeneration.
Traditional
wound
methods
include
the
use
synthetic
materials
such
as
petroleum-based
ointments
plastic
films.
However,
these
can
be
associated
negative
environmental
impacts
may
not
biocompatible
human
body.
Tissue
engineering
has
emerged
promising
approach
to
treating
burns,
sustainable
biomaterials
have
been
developed
an
alternative
option.
Green
collagen,
cellulose,
chitosan,
others
biocompatible,
biodegradable,
environment-friendly,
cost-effective,
which
reduces
impact
their
production
disposal.
They
effective
promoting
healing
risk
infection
other
benefits
inflammation
angiogenesis.
This
comprehensive
review
focuses
on
multifunctional
green
that
potential
revolutionize
way
we
treat
skin
faster
more
efficient
while
minimizing
damage.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 18, 2024
A
favorable
regenerative
microenvironment
is
essential
for
peripheral
nerve
regeneration.
Neural
tissue-specific
extracellular
matrix
(ECM)
a
natural
material
that
helps
direct
cell
behavior
and
promote
axon
Both
bone
marrow-derived
mesenchymal
stem
cells
(BMSCs)
adipose-derived
(ADSCs)
transplantation
are
effective
in
repairing
injury
(PNI).
However,
there
no
study
characterizes
the
vivo
microenvironmental
characteristics
of
these
two
MSCs
early
repair
PNI
when
combined
with
neural
tissue-derived
ECM
materials,
i.e.,
acellular
allograft
(ANA).
In
order
to
investigate
biological
characteristics,
molecular
mechanisms
stage,
effectiveness
ADSCs-
or
BMSCs-injected
into
ANA
vivo,
rat
10
mm
long
sciatic
defect
model
was
used.
We
isolated
primary
BMSCs
ADSCs
from
marrow
adipose
tissue,
respectively.
First,
response
underlying
ADSCs,
eighty-four
rats
were
randomly
divided
three
groups:
group,
ANA+BMSC
ANA+ADSC
group.
performed
flow
cytometry,
RT-PCR,
immunofluorescence
staining
up
4
weeks
postoperatively.
To
further
elucidate
mechanisms,
changes
noncoding
RNAs
(lncRNAs),
circular
(circRNAs),
microRNAs
(miRNAs),
messenger
(mRNAs)
systematically
investigated
using
whole
transcriptome
sequencing.
then
constructed
protein-protein
interaction
networks
find
top
ranked
hub
genes
among
differentially
expressed
mRNAs.
Second,
explore
on
materials
PNI,
sixty-eight
randomized
four
AUTO
groups,
ADSCs/BMSCs
equally
injected
along
axis
10-mm
ANA.
Then,
we
histological
functional
assessments
12
The
results
cytometry
RT-PCR
showed
exhibited
more
significant
immunomodulatory
effects,
as
evidenced
by
up-regulation
interleukin
(IL)-10,
down-regulation
IL-1β
tumor
necrosis
factor-alpha
(TNF-α)
expression,
promotion
M1-type
macrophage
polarization
M2-type,
increase
number
regulatory
T
(Tregs).
pronounced
features
pro-myelination
angiogenesis,
myelin-associated
protein
gene
(MBP
MPZ)
angiogenesis-related
factors
(TGF-β,
VEGF).
Moreover,
sequencing
indicated
loaded
notable
effects
associated
axonal
growth,
myelin
formation.
Notably,
infused
enhanced
regeneration
motor
function
recovery
statistically
differences.
This
revealed
both
enhance
recovery,
but
their
(mainly
including
pro-vascular
pro-myelin
effects)
process
different,
providing
new
insights
MSC
therapy
its
clinical
translation.
Journal of Biomedical Materials Research Part A,
Год журнала:
2023,
Номер
112(5), С. 640 - 671
Опубликована: Ноя. 22, 2023
Abstract
By
removing
the
immunogenic
cellular
components
through
various
decellularization
methods,
decellularized
extracellular
matrix
(dECM)
is
considered
a
promising
material
in
field
of
tissue
engineering
and
regenerative
medicine
with
highly
preserved
physicochemical
properties
superior
biocompatibility.
However,
treatment
can
lead
to
some
loss
structural
integrity,
mechanical
strength,
degradation
stability,
biological
performance
dECM
biomaterials.
Therefore,
physical
chemical
crosslinking
methods
are
preferred
restore
or
even
improve
biomechanical
properties,
bioactivity,
achieve
delicate
balance
between
implanted
biomaterial
regeneration
host
tissue.
This
review
provides
an
overview
biomaterials,
describes
compares
mechanisms
characteristics
commonly
used
for
dECM,
focus
on
potential
applications
versatile
dECM‐based
biomaterials
derived
from
skin,
cardiac
tissues
(pericardium,
heart
valves,
myocardial
tissue),
blood
vessels,
liver,
kidney,
modified
different
reagents,
organ
regeneration.
Journal of Tissue Viability,
Год журнала:
2025,
Номер
34(2), С. 100870 - 100870
Опубликована: Фев. 8, 2025
More
than
5
%
of
people
worldwide
suffer
from
hearing
disorders.
Ototoxic
drugs,
aging,
exposure
to
loud
sounds,
rupture,
subperichondrial
hematoma,
perichondritis,
burns
and
frostbite
infections
are
the
main
causes
loss,
some
which
can
destroy
cartilage
lead
deformation.
On
other
hand,
disorders
external
ear
diverse
range
dangerous
neoplasms
defects
that
not
acceptable
a
cosmetic
standpoint.
These
issues
include
injuries,
blockages,
dermatoses,
infections,
any
or
all
them
may
be
bothersome
busy
doctor.
Using
an
implant
aid
is
one
treatment
strategies
for
deafness.
However,
these
medical
devices
useful
every
eligible
patient.
With
right
therapy,
many
life-threatening
treated
with
confidence
in
positive
outcome.
As
research
have
advanced
dramatically
past
ten
years,
tissue
engineering
(TE)
has
emerged
as
promising
method
regenerate
damaged
tissue,
raising
prospect
permanent
cure
Decellularization
now
seen
development
regenerative
medicine,
increasing
number
applications
being
found
acellular
matrices.
Studies
on
decellularization
show
natural
scaffolds
made
decellularized
tissues
serve
suitable
platform
while
preserving
components,
preparation
such
will
important
part
future
bioscience
research.
It
wide
medicine
TE.
This
review
intends
give
overview
status
alternative
inner
outer
both
preclinical
clinical
perspective
order
how
ongoing
TE
potential
advance
enhance
novel
disease
treatments.
