Decellularized Extracellular Matrix for Remodeling Bioengineering Organoid's Microenvironment

Small, Journal Year: 2023, Volume and Issue: 19(25)

Published: March 16, 2023

Abstract Over the past decade, stem cell‐ and tumor‐derived organoids are most promising models in developmental biology disease modeling, respectively. The matrix is one of three main elements construction an organoid important module its extracellular microenvironment. However, source currently available commercial matrix, Matrigel, limits application clinical medicine. It worth investigating whether original decellularized (dECM) can be exploited as improving very important. In this review, tissue decellularization protocols characteristics methods, mechanical support biological cues extraccellular (ECM), methods for multifunctional dECM responsive hydrogel, potential applications functional summarized. addition, some expectations provided applications.

Language: Английский

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Macrophage plasticity: signaling pathways, tissue repair, and regeneration

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: Aug. 1, 2024

Abstract Macrophages are versatile immune cells with remarkable plasticity, enabling them to adapt diverse tissue microenvironments and perform various functions. Traditionally categorized into classically activated (M1) alternatively (M2) phenotypes, recent advances have revealed a spectrum of macrophage activation states that extend beyond this dichotomy. The complex interplay signaling pathways, transcriptional regulators, epigenetic modifications orchestrates polarization, allowing respond stimuli dynamically. Here, we provide comprehensive overview the cascades governing focusing on roles Toll‐like receptors, signal transducer activator transcription proteins, nuclear microRNAs. We also discuss emerging concepts metabolic reprogramming trained immunity, contributing their functional adaptability. Macrophage plasticity plays pivotal role in repair regeneration, macrophages coordinating inflammation, angiogenesis, matrix remodeling restore homeostasis. By harnessing potential novel therapeutic strategies targeting polarization could be developed for diseases, including chronic wounds, fibrotic disorders, inflammatory conditions. Ultimately, deeper understanding molecular mechanisms underpinning will pave way innovative regenerative medicine engineering approaches.

Language: Английский

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Functional acellular matrix for tissue repair

Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100530 - 100530

Published: Dec. 28, 2022

In view of their low immunogenicity, biomimetic internal environment, tissue- and organ-like physicochemical properties, functionalization potential, decellularized extracellular matrix (dECM) materials attract considerable attention are widely used in tissue engineering. This review describes the composition matrices role stem-cell differentiation, discusses advantages disadvantages existing decellularization techniques, presents methods for characterization scaffolds. addition, we discuss progress use dECMs cartilage, skin, nerve, muscle repair transplantation or regeneration different whole organs (e.g., kidneys, liver, uterus, lungs, heart), summarize shortcomings using organ after refunctionalization, examine corresponding future prospects. Thus, present helps to further systematize application functionalized tissue/organ keep researchers up date on recent dECM usage.

Language: Английский

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Decellularized Extracellular Matrix Scaffolds for Cardiovascular Tissue Engineering: Current Techniques and Challenges

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 13040 - 13040

Published: Oct. 27, 2022

Cardiovascular diseases are the leading cause of global mortality. Over past two decades, researchers have tried to provide novel solutions for end-stage heart failure address cardiac transplantation hurdles such as donor organ shortage, chronic rejection, and life-long immunosuppression. Cardiac decellularized extracellular matrix (dECM) has been widely explored a promising approach in tissue-regenerative medicine because its remarkable similarity original tissue. Optimized decellularization protocols combining physical, chemical, enzymatic agents developed obtain perfect balance between cell removal, ECM composition, function maintenance. However, proper assessment tissue composition is still needed before clinical translation. Recellularizing acellular scaffold with organ-specific cells evaluating extent cardiomyocyte repopulation also challenging. This review aims discuss existing literature on scaffolds, especially advantages methods preparation, pointing out areas improvement. Finally, an overview state research regarding application dECM future challenges bioengineering human suitable provided.

Language: Английский

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Progress in biomaterials inspired by the extracellular matrix

Giant, Journal Year: 2024, Volume and Issue: 19, P. 100323 - 100323

Published: July 10, 2024

Inspired by the extracellular matrix (ECM), biomaterials have emerged as promising strategies in biomedical research and engineering domain, offering unique characteristics for tissue regeneration, drug delivery, therapeutic interventions, cellular investigations. The ECM, a dynamic network structure secreted various cells, primarily comprises diverse proteins capable of facilitating tissue-ECM signaling regulatory functions through its rich array bioactive substances multi-level structural properties. Drawing inspiration from intricate biochemical composition natural researchers developed to encapsulate these features create biomimetic microenvironments, such electrospinning, hydrogels/hydrogel microspheres, decellularized ECM(dECM), ECM-mimicking peptides. Furthermore, mimicking ECM components, ECM-inspired exhibit varying degrees functionalization, including providing support, cell adhesion, signal transduction, mitigating immune responses, remodeling. In summary, advancements offer significant promise addressing key challenges fields engineering, regenerative medicine, delivery.

Language: Английский

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Decellularized kidney extracellular matrix-based hydrogels for renal tissue engineering

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 180, P. 295 - 307

Published: April 18, 2024

Kidney regeneration is hindered by the limited pool of intrinsic reparative cells. Advanced therapies targeting renal have potential to alleviate clinical and financial burdens associated with kidney disease. Delivery systems for cells, extracellular vesicles, or growth factors aimed at enhancing can benefit from vehicles enabling targeted delivery controlled release. Hydrogels, optimized carry biological cargo while promoting regeneration, emerged as promising candidates this purpose. This aims develop a hydrogel decellularized matrix (DKECM) explore its biocompatibility biomaterial regeneration. The resulting crosslinks temperature exhibits high concentration matrix. decellularization process efficiently removes detergent residues, yielding pathogen-free that non-hemolytic devoid α-gal epitope. Upon interaction macrophages, induces differentiation into both pro-inflammatory anti-inflammatory phenotypes, suggesting an adequate balance promote functionality in vivo. Renal progenitor cells encapsulated DKECM demonstrate higher viability proliferation than commercial collagen-I hydrogels, also expressing tubular podocyte markers long-term culture. Overall, injectable derived porcine anticipated elicit minimal host reaction fostering cell bioactivity, offering avenue settings. quest improve treatments disease crucial, given challenges faced patients on dialysis waiting transplants. Exciting new combining biomaterials revolutionize repair. In study, researchers created pig kidney. gel could be used deliver other substances help Despite coming pigs, it's safe use humans, no harmful reduced risk immune reactions. Importantly, it promotes balanced healing response body. research not only advances our knowledge repair but offers hope more effective diseases.

Language: Английский

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Recent Advances in Decellularized Extracellular Matrix-Based Bioinks for 3D Bioprinting in Tissue Engineering

Materials, Journal Year: 2023, Volume and Issue: 16(8), P. 3197 - 3197

Published: April 18, 2023

In recent years, three-dimensional (3D) bioprinting has been widely utilized as a novel manufacturing technique by more and researchers to construct various tissue substitutes with complex architectures geometries. Different biomaterials, including natural synthetic materials, have manufactured into bioinks for regeneration using 3D bioprinting. Among the biomaterials derived from tissues or organs, decellularized extracellular matrix (dECM) internal structure variety of bioactive factors that provide mechanistic, biophysical, biochemical signals remodeling. developing dECM bioink construction substitutes. Compared other bioinks, ECM components in dECM-based can regulate cellular functions, modulate process, adjust Therefore, we conducted this review discuss current status perspectives on engineering. addition, techniques decellularization methods were also discussed study.

Language: Английский

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Injectable decellularized extracellular matrix hydrogel promotes salivary gland regeneration via endogenous stem cell recruitment and suppression of fibrogenesis

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 169, P. 256 - 272

Published: Aug. 8, 2023

Language: Английский

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Three Birds, One Stone: An Osteo‐Microenvironment Stage‐Regulative Scaffold for Bone Defect Repair through Modulating Early Osteo‐Immunomodulation, Middle Neovascularization, and Later Osteogenesis

Advanced Science, Journal Year: 2023, Volume and Issue: 11(6)

Published: Dec. 7, 2023

Abstract In order to repair critical‐sized bone defects, various polylactic acid‐glycolic acid (PLGA)‐based hybrid scaffolds are successfully developed as substitutes. However, the byproducts of these PLGA‐based known acidify implanted site, inducing tiresome acidic inflammation. Moreover, degradation productions cannot offer an osteo‐friendly microenvironment at matching natural healing. Herein, inspired by atlas bone‐healing process, osteo‐microenvironment stage‐regulative scaffold (P80/D10/M10) is fabricated incorporating self‐developed decellularized matrix microparticles (DBM‐MPs) and multifunctional magnesium hydroxide nanoparticles (MH‐NPs) into PLGA with optimized proportion using low‐temperature rapid prototyping (LT‐RP) 3D‐printing technology. The cell experiments show that this P80/D10/M10 exhibits excellent properties in mechanics, biocompatibility, biodegradability, meanwhile superior stimulations osteo‐immunomodulation, angiogenesis, osteogenesis. Additionally, animal determined can a stage‐matched pattern for enhanced regeneration, namely, optimization early inflammation, middle neovascularization, later formation. Furthermore, transcriptomic analysis suggested vivo performance on defect mostly attributed regulating artery development, remodeling. Overall, study reveals provides promising treatment repair.

Language: Английский

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Intestinal organoid modeling: bridging the gap from experimental model to clinical translation

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: March 1, 2024

The 3D culture of intestinal organoids entails embedding isolated crypts and bone marrow mesenchymal stem cells within a growth factor-enriched matrix gel. This process leads to the formation hollow microspheres with structures resembling epithelial cells, which are referred as organoids. These encompass various functional cell types found in small intestine closely mimic organizational patterns intestine, earning them name “mini-intestines”. Intestinal tumors prevalent digestive system represent significant menace human health. Through application technology, miniature colorectal organs can be cultivated retain genetic characteristics primary tumor. innovation offers novel prospects for individualized treatments among patients tumors. Presently established libraries patient-derived serve potent tools conducting comprehensive investigations into tissue functionality, developmental processes, tumorigenesis, pathobiology cancer. review explores origins organoids, their culturing environments, advancements realm precision medicine. It also addresses current challenges outlines future development.

Language: Английский

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