A Microfluidic Approach for Synthesis of Silver Nanoparticles as a Potential Antimicrobial Agent in Alginate–Hyaluronic Acid-Based Wound Dressings DOI Open Access
Alexandra Cătălina Bîrcă, Oana Gherasim, Adelina-Gabriela Niculescu

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(14), С. 11466 - 11466

Опубликована: Июль 14, 2023

The recognized antimicrobial activity of silver nanoparticles is a well-studied property, especially when designing and developing biomaterials with medical applications. As biological closely related to the physicochemical characteristics material, aspects such as particle morphology dimension should be considered. Microfluidic systems in continuous flow represent promising method control size, shape, size distribution synthesized nanoparticles. Moreover, using microfluidics widens synthesis options by creating controlling parameters that are otherwise difficult maintain conventional batch procedures. This study used microfluidic platform cross-shape design an innovative for synthesizing varied precursor concentration purging speed experimental parameters. compositional microstructural characterization obtained samples was carried out X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission (TEM), dynamic light scattering (DLS). Four formulations alginate-based hydrogels addition hyaluronic acid were highlight efficiency composite wound treatment. porous structure, swelling capacity, properties evaluated through analysis (FT-IR SEM) contact prokaryotic eukaryotic cells. results investigations revealed desirable performant dressings (i.e., biocompatibility, appropriate rate, degradation ability inhibit biofilm formation, cell growth stimulation capacity), materials thus recommended treating chronic infected wounds.

Язык: Английский

Green synthesized ZnO nanoparticles by Saccharomyces cerevisiae and their antibacterial activity and photocatalytic degradation DOI Creative Commons
Ahmed M. El‐Khawaga, Mohamed A. Elsayed, Mohamed Gobara

и другие.

Biomass Conversion and Biorefinery, Год журнала: 2023, Номер unknown

Опубликована: Сен. 15, 2023

Abstract Many research papers are currently being written about various methodologies for the green synthesis of ZnO nanoparticles. In this study, nanoparticles were prepared by method using baker’s yeast ( Saccharomyces cerevisiae ). The characterization produced involved utilization XRD, FTIR, SEM, and TEM. investigation assessment antimicrobial potential photocatalytic degradation efficacy synthesized materials through Eriochrome Black T (EBT). results indicate that exhibit a spherical morphology, exhibiting diameters ranging from 13.0 to 20.0nm, with an average particle size 15.0nm. against S. aureus ) gram-positive E. coli gram-negative bacteria was tested conduction zone inhibition (ZOI) minimum inhibitory concentration (MIC) tests. This focused on study photocatalytic-degradation EBT in presence ultraviolet light. experiments impact factors, such as pH levels, initial concentration, nanocatalyst dosage, observed efficiency. vitro ZOI MIC assay, it (NPs) exhibited greater , measuring 23.1 mm 0.625 μg/ml. contrast, effectiveness ZnO-NPs comparatively lower, indicated 17.0 1.250μg/ml. greatest elimination, which achieved 83.0% equilibrium, found employing 20.0mg NPs at 3.0, according activity results. synthetic powerful antibacterial agents effective may be used treatment wastewater.

Язык: Английский

Процитировано

63
Harmonizing nature and nanotechnology: Phytoextract-mediated synthesis of Ag-doped ZnO nanoparticles using Lavandula stoechas extract for environmental and biomedical applications DOI
Zahra Kiani, Seyedsina Mirjalili, Kamran Heydaryan

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 96, С. 105708 - 105708

Опубликована: Апрель 23, 2024

Язык: Английский

Процитировано

53
Process optimization for green synthesis of silver nanoparticles using Rubus discolor leaves extract and its biological activities against multi-drug resistant bacteria and cancer cells DOI Creative Commons
Saeed Ghasemi, Sara Dabirian,

Faezeh Kariminejad

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Фев. 19, 2024

Abstract Multi-drug resistant (MDR) bacteria are considered a serious public health threat. Also, increasing rate of resistance to anticancer drugs, as well their toxicity, is another point concern. Therefore, the new antibacterial and agents always needed. The synthesizing silver nanoparticles (AgNPs) using medicinal plants, an effective approach for developing novel agents. Rubus discolor , native species Caucasus region, produces leaves that typically discarded by-product raspberry production. present study has focused on optimizing green synthesis AgNPs R. extract through response surface methodology. optimal values were AgNO 3 concentration 7.11 mM, time 17.83 h, temperature 56.51 °C, percentage 29.22. production was confirmed UV–visible spectroscopy (λ max at 456.01 nm). TEM analysis revealed well-dispersed (an average size 37 XRD crystalline structure. EDX detected strong peak keV corresponded Ag. zeta potential value (− 44.2 mV) indicated stability nanoparticles. FT-IR spectra showed presence various functional groups from plant compounds, which play important role in capping bio-reduction processes. impressive activities against MDR Escherichia coli Pseudomonas aeruginosa (MIC ranging 0.93 3.75 mg ml −1 ). phytochemical phenolics, tannins, flavonoids AgNPs. They also significant cytotoxic effects A431, MCF-7, HepG2 cells (IC 50 11 49.1 µg −l

Язык: Английский

Процитировано

45
Phyco-synthesis of silver nanoparticles by environmentally safe approach and their applications DOI Creative Commons

Sunita Choudhary,

Geetanjali Kumawat,

Manisha Khandelwal

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Апрель 26, 2024

In recent years, there has been an increasing interest in the green synthesis of metallic nanoparticles, mostly because evident limitations associated with chemical and physical methods. Green synthesis, commonly referred to as "biogenic synthesis," is seen alternative approach produce AgNPs (silver nanoparticles). The current work focuses on use Asterarcys sp. (microalga) for biological reduction AgNO

Язык: Английский

Процитировано

25
Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62 DOI Creative Commons
Fathy M. Elkady, Amr H. Hashem, Salem S. Salem

и другие.

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Март 8, 2024

Abstract Background and objectives Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed. Methodology St/Ag-Se NC was using Cladosporium cladosporioides CBS 174.62 ( C. ) strain. shape average particle size investigated scanning electron microscope (SEM) high-resolution transmission (HR-TEM), respectively. On other hand, on two cancer cell lines red blood (RBCs) evaluated its hydrogen peroxide (H 2 O scavenging Moreover, effects microbial species in both planktonic biofilm growth forms examined. Results successfully oval spherical mean diameter 67.87 nm as confirmed by HR-TEM analysis. showed promising anticancer activity toward human colorectal carcinoma (HCT-116) breast (MCF-7) where IC 50 21.37 19.98 µg/ml, Similarly, little RBCs observed low concentration. As well, highest H (42.84%) recorded at concentration mg/ml. Additionally, Staphylococcus epidermidis S. ATCC 12,228 Candida albicans 10,231 highly affected bacterial fungal strains minimum inhibitory concentrations (MICs) 18.75 noticeable dependent. A high suppression percentage, 87.5% 68.05%, aureus when exposed 1 mg/ml 0.5 mg/ml, Conclusion excellent antioxidant activity, haemocompatibility, anti-proliferative concentrations. Also, it exhibited antibiofilm activities.

Язык: Английский

Процитировано

17
Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity DOI Open Access
Nilly A. H. Abdelfattah,

Manar Ali Yousef,

Ali A. Badawy

и другие.

Biotechnology Journal, Год журнала: 2023, Номер 18(12)

Опубликована: Авг. 24, 2023

Recently, agricultural management innovation has incorporated engineered nanoparticles. The current investigation was carried out to produce magnesium oxide nanoparticles (MgONPs) for the first time applying S. cerevisiae extract. FTIR, XRD, HRTEM, and zeta potential analysis were used characterize MgONPs. FTIR data show that bioactive substances reduce cap synthesized crystalline metallic MgONPs had four significant peaks in XRD pattern. size form of validated by TEM, which exhibited spherical structures with an average 27 nm. effect various dosages administered cowpea (Vigna unguiculata L.) plant on all vitro parameters shown be study. concentration 200 ppm most treatment increased shoot length, dry-weight root 27.35%, 45.09%, 31.91% when compared untreated plants. significantly photosynthetic pigments, 150 increasing soluble proteins carbohydrates. effectively treated C. maculatus, dose time-dependent insecticidal activity. death rates varied 82.66% 100% fifth day. Biochemical histopathological studies rats investigated. Rats showed higher GOT, GPT, Urea levels, but lower creatinine, indicating differences. MgONPs-treated rats' liver mild moderate histopathologic changes, including portal blood vessel congestion, lymphocytic cholangitis, degenerative changes. improve development outcomes suppress grain insects (C. maculatus).

Язык: Английский

Процитировано

40
Recent Insights into Nanotechnology in Colorectal Cancer DOI
Aram Asareh Zadegan Dezfuli, Mohammed Abu‐Elghait, Salem S. Salem

и другие.

Applied Biochemistry and Biotechnology, Год журнала: 2023, Номер 196(7), С. 4457 - 4471

Опубликована: Сен. 26, 2023

Язык: Английский

Процитировано

30
Utilization of biosynthesized silver nanoparticles from Agaricus bisporus extract for food safety application: synthesis, characterization, antimicrobial efficacy, and toxicological assessment DOI Creative Commons

Maryam Amr,

Samah H. Abu-Hussien,

Radwa Ismail

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Сен. 12, 2023

The emergence of antimicrobial resistance in foodborne bacterial pathogens has raised significant concerns the food industry. This study explores potential biosynthesized silver nanoparticles (AgNPs) derived from Agaricus bisporus (Mushroom) against pathogens. AgNPs were characterized using various techniques, including UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared high-resolution scanning electron microscopy with energy dispersive dynamic light scattering, and zeta analysis. antibacterial activity was tested a panel strains, their cytotoxicity evaluated on normal human skin fibroblasts. Among Pseudomonas aeruginosa ATCC 27853 showed highest sensitivity an inhibition zone diameter (IZD) 48 mm, while Klebsiella quasipneumoniae ATTC 700603 Bacillus cereus 11778 displayed IZDs 20 mm. cations released by demonstrated strong bactericidal effects both Gram-positive (G + ve) Gram-negative - bacteria, as evidenced minimum inhibitory concentration/minimum concentration (MBC/MIC) ratio. Moreover, testing fibroblasts (HSF) indicated that mushroom extract safe, cell viability 98.2%. Therefore, hold promise alternative means to inhibit biofilm formation industry sector.

Язык: Английский

Процитировано

25
Biogenic Nanomaterials: Synthesis, Characterization, and Applications DOI
Salem S. Salem, Alsayed E. Mekky

Environmental science and engineering, Год журнала: 2024, Номер unknown, С. 13 - 43

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

12
Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus DOI Creative Commons
Ahmed N. Alabssawy, Mohammed Abu‐Elghait, Ahmad M. Azab

и другие.

BMC Biotechnology, Год журнала: 2024, Номер 24(1)

Опубликована: Май 4, 2024

Abstract Scientists know very little about the mechanisms underlying fish skin mucus, despite fact that it is a component of immune system. Fish mucus an important defence against invasive infections. Recently, and its are gaining interest among immunologists. Characterization was done on obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal proteins (EMP-Ag-NPs) through UV–vis, FTIR, XRD, TEM, SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical form angles were 38.10°, 44.20°, 64.40°, 77.20°, Where wavelength change after formation EMP-Ag-NPs as indicate dark brown, broad band recorded at 391 nm. Additionally, antimicrobial, antibiofilm anticancer activities assessed. The present results demonstrate high activity unicellular fungi C. albicans , followed by E. faecalis . Antibiofilm showed strong both S. aureus P. aeruginosa pathogens dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect investigated normal cells (Vero), breast cancer (Mcf7) hepatic carcinoma (HepG2) lines concentrations (200–6.25 µg/mL) current highly 100, 5 25 µg/mL exhibited rounding, shrinkage, deformation granulation Mcf7 HepG2 IC50 19.34 31.16 respectively while Vero appeared rounded concentration 50 shape 25, 12.5 6.25 µg/ml 35.85 µg/mL. This study evidence potential efficacy biologically generated substitute medicinal agent harmful microorganisms. Furthermore, highlights their inhibitory lines.

Язык: Английский

Процитировано

11