Response surface methodology: Optimization of myco-synthesized gold and silver nanoparticles by Trichoderma saturnisporum DOI Creative Commons
Mohammed Abu‐Elghait, Mohamed K. Y. Soliman, Mohamed Salah Azab

et al.

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 23, 2023

Abstract In the current study, optimal reaction condition for fabrication of Au and Ag nanoparticles by using Trichoderma saturnisporum was developed. Optimization fermentation conditions extracellular AuNPs AgNPs synthesis response surface methodology achieved. To accomplish ideal state, green reaction’s effective parameters central composite face design were used. Precursor concentrations, pH, temperature found to be most nanomaterials. The model’s realization used a respectable foot. Normal probability plots; interaction plots, including effect variance analysis (ANOVA), contour Pareto charts optimize components. Significance threshold (5%) influence all factors. production process also influenced some potential connections between these recommended regression model fits experimental data extremely well. findings this study looked at how much more bio-fabrication possible with producer compared traditional optimization techniques.

Language: Английский

Green synthesized ZnO nanoparticles by Saccharomyces cerevisiae and their antibacterial activity and photocatalytic degradation DOI Creative Commons
Ahmed M. El‐Khawaga, Mohamed A. Elsayed, Mohamed Gobara

et al.

Biomass Conversion and Biorefinery, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 15, 2023

Abstract Many research papers are currently being written about various methodologies for the green synthesis of ZnO nanoparticles. In this study, nanoparticles were prepared by method using baker’s yeast ( Saccharomyces cerevisiae ). The characterization produced involved utilization XRD, FTIR, SEM, and TEM. investigation assessment antimicrobial potential photocatalytic degradation efficacy synthesized materials through Eriochrome Black T (EBT). results indicate that exhibit a spherical morphology, exhibiting diameters ranging from 13.0 to 20.0nm, with an average particle size 15.0nm. against S. aureus ) gram-positive E. coli gram-negative bacteria was tested conduction zone inhibition (ZOI) minimum inhibitory concentration (MIC) tests. This focused on study photocatalytic-degradation EBT in presence ultraviolet light. experiments impact factors, such as pH levels, initial concentration, nanocatalyst dosage, observed efficiency. vitro ZOI MIC assay, it (NPs) exhibited greater , measuring 23.1 mm 0.625 μg/ml. contrast, effectiveness ZnO-NPs comparatively lower, indicated 17.0 1.250μg/ml. greatest elimination, which achieved 83.0% equilibrium, found employing 20.0mg NPs at 3.0, according activity results. synthetic powerful antibacterial agents effective may be used treatment wastewater.

Language: Английский

Citations

71
Harmonizing nature and nanotechnology: Phytoextract-mediated synthesis of Ag-doped ZnO nanoparticles using Lavandula stoechas extract for environmental and biomedical applications DOI
Zahra Kiani, Seyedsina Mirjalili, Kamran Heydaryan

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 96, P. 105708 - 105708

Published: April 23, 2024

Language: Английский

Citations

55
Process optimization for green synthesis of silver nanoparticles using Rubus discolor leaves extract and its biological activities against multi-drug resistant bacteria and cancer cells DOI Creative Commons
Saeed Ghasemi, Sara Dabirian,

Faezeh Kariminejad

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 19, 2024

Abstract Multi-drug resistant (MDR) bacteria are considered a serious public health threat. Also, increasing rate of resistance to anticancer drugs, as well their toxicity, is another point concern. Therefore, the new antibacterial and agents always needed. The synthesizing silver nanoparticles (AgNPs) using medicinal plants, an effective approach for developing novel agents. Rubus discolor , native species Caucasus region, produces leaves that typically discarded by-product raspberry production. present study has focused on optimizing green synthesis AgNPs R. extract through response surface methodology. optimal values were AgNO 3 concentration 7.11 mM, time 17.83 h, temperature 56.51 °C, percentage 29.22. production was confirmed UV–visible spectroscopy (λ max at 456.01 nm). TEM analysis revealed well-dispersed (an average size 37 XRD crystalline structure. EDX detected strong peak keV corresponded Ag. zeta potential value (− 44.2 mV) indicated stability nanoparticles. FT-IR spectra showed presence various functional groups from plant compounds, which play important role in capping bio-reduction processes. impressive activities against MDR Escherichia coli Pseudomonas aeruginosa (MIC ranging 0.93 3.75 mg ml −1 ). phytochemical phenolics, tannins, flavonoids AgNPs. They also significant cytotoxic effects A431, MCF-7, HepG2 cells (IC 50 11 49.1 µg −l

Language: Английский

Citations

49
Phyco-synthesis of silver nanoparticles by environmentally safe approach and their applications DOI Creative Commons

Sunita Choudhary,

Geetanjali Kumawat,

Manisha Khandelwal

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 26, 2024

In recent years, there has been an increasing interest in the green synthesis of metallic nanoparticles, mostly because evident limitations associated with chemical and physical methods. Green synthesis, commonly referred to as "biogenic synthesis," is seen alternative approach produce AgNPs (silver nanoparticles). The current work focuses on use Asterarcys sp. (microalga) for biological reduction AgNO

Language: Английский

Citations

26
Unveiling biological activities of biosynthesized starch/silver-selenium nanocomposite using Cladosporium cladosporioides CBS 174.62 DOI Creative Commons
Fathy M. Elkady, Amr H. Hashem, Salem S. Salem

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: March 8, 2024

Abstract Background and objectives Microbial cells capability to tolerate the effect of various antimicrobial classes represent a major worldwide health concern. The flexible multi-components nanocomposites have enhanced physicochemical characters with several improved properties. Thus, different biological activities biosynthesized starch/silver-selenium nanocomposite (St/Ag-Se NC) were assessed. Methodology St/Ag-Se NC was using Cladosporium cladosporioides CBS 174.62 ( C. ) strain. shape average particle size investigated scanning electron microscope (SEM) high-resolution transmission (HR-TEM), respectively. On other hand, on two cancer cell lines red blood (RBCs) evaluated its hydrogen peroxide (H 2 O scavenging Moreover, effects microbial species in both planktonic biofilm growth forms examined. Results successfully oval spherical mean diameter 67.87 nm as confirmed by HR-TEM analysis. showed promising anticancer activity toward human colorectal carcinoma (HCT-116) breast (MCF-7) where IC 50 21.37 19.98 µg/ml, Similarly, little RBCs observed low concentration. As well, highest H (42.84%) recorded at concentration mg/ml. Additionally, Staphylococcus epidermidis S. ATCC 12,228 Candida albicans 10,231 highly affected bacterial fungal strains minimum inhibitory concentrations (MICs) 18.75 noticeable dependent. A high suppression percentage, 87.5% 68.05%, aureus when exposed 1 mg/ml 0.5 mg/ml, Conclusion excellent antioxidant activity, haemocompatibility, anti-proliferative concentrations. Also, it exhibited antibiofilm activities.

Language: Английский

Citations

17
Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties DOI Creative Commons
Amr H. Hashem, Mostafa A. Abdel-Maksoud, Sabiha Fatima

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Cancer and microbial infections place a significant burden on the world's health systems can increase rate of disease mortality. In current study, novel nanocomposite based Gum Arabic, silver copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome problem infection in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, observed peak spectrum formed by O.D. at 0.755, confirmed that produced GA@Ag-CuO small discernible 360 nm. The particles' diameters varied from 9.5 nm 49.5 nm, with mean diameter 25.53 ± 1.4 created Arabic filtrate rich active functional groups, provided polydisperse NPs were intended reduce, stabilize, act as capping agents. Based XRD data, crystallized had face-centered (fcc) crystal structure. Biosafety assessed toward Wi 38 normal cell line, where it showed safety tested line IC50 154.2 µg/mL. Antimicrobial results has antibacterial activity MICs 15.6, 125, 31.25 125 µg/mL against S. epidermis, aureus, L. plantrum, typhimurium, respectively. Likewise, antifungal C. albicans neoformans 62.5 15.62 µg/ml, Moreover, displayed promising anticancer 26.11 59.5 µg/ml MCF-7 Hep-G2, conclusion, demonstrated antibacterial, antifungal, activities.

Language: Английский

Citations

2
A Comprehensive Review on Exploring the Potential of Phytochemicals and Biogenic Nanoparticles for the Treatment of Antimicrobial-Resistant Pathogenic Bacteria DOI

Shibasini Murugan,

T. Senthilvelan,

Mani Govindasamy

et al.

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(2)

Published: Jan. 18, 2025

Language: Английский

Citations

2
Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity DOI Open Access
Nilly A. H. Abdelfattah,

Manar Ali Yousef,

Ali A. Badawy

et al.

Biotechnology Journal, Journal Year: 2023, Volume and Issue: 18(12)

Published: Aug. 24, 2023

Recently, agricultural management innovation has incorporated engineered nanoparticles. The current investigation was carried out to produce magnesium oxide nanoparticles (MgONPs) for the first time applying S. cerevisiae extract. FTIR, XRD, HRTEM, and zeta potential analysis were used characterize MgONPs. FTIR data show that bioactive substances reduce cap synthesized crystalline metallic MgONPs had four significant peaks in XRD pattern. size form of validated by TEM, which exhibited spherical structures with an average 27 nm. effect various dosages administered cowpea (Vigna unguiculata L.) plant on all vitro parameters shown be study. concentration 200 ppm most treatment increased shoot length, dry-weight root 27.35%, 45.09%, 31.91% when compared untreated plants. significantly photosynthetic pigments, 150 increasing soluble proteins carbohydrates. effectively treated C. maculatus, dose time-dependent insecticidal activity. death rates varied 82.66% 100% fifth day. Biochemical histopathological studies rats investigated. Rats showed higher GOT, GPT, Urea levels, but lower creatinine, indicating differences. MgONPs-treated rats' liver mild moderate histopathologic changes, including portal blood vessel congestion, lymphocytic cholangitis, degenerative changes. improve development outcomes suppress grain insects (C. maculatus).

Language: Английский

Citations

40
Recent Insights into Nanotechnology in Colorectal Cancer DOI
Aram Asareh Zadegan Dezfuli, Mohammed Abu‐Elghait, Salem S. Salem

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 196(7), P. 4457 - 4471

Published: Sept. 26, 2023

Language: Английский

Citations

31
Utilization of biosynthesized silver nanoparticles from Agaricus bisporus extract for food safety application: synthesis, characterization, antimicrobial efficacy, and toxicological assessment DOI Creative Commons

Maryam Amr,

Samah H. Abu-Hussien,

Radwa Ismail

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 12, 2023

The emergence of antimicrobial resistance in foodborne bacterial pathogens has raised significant concerns the food industry. This study explores potential biosynthesized silver nanoparticles (AgNPs) derived from Agaricus bisporus (Mushroom) against pathogens. AgNPs were characterized using various techniques, including UV-visible spectroscopy, X-ray diffraction, Fourier transform infrared high-resolution scanning electron microscopy with energy dispersive dynamic light scattering, and zeta analysis. antibacterial activity was tested a panel strains, their cytotoxicity evaluated on normal human skin fibroblasts. Among Pseudomonas aeruginosa ATCC 27853 showed highest sensitivity an inhibition zone diameter (IZD) 48 mm, while Klebsiella quasipneumoniae ATTC 700603 Bacillus cereus 11778 displayed IZDs 20 mm. cations released by demonstrated strong bactericidal effects both Gram-positive (G + ve) Gram-negative - bacteria, as evidenced minimum inhibitory concentration/minimum concentration (MBC/MIC) ratio. Moreover, testing fibroblasts (HSF) indicated that mushroom extract safe, cell viability 98.2%. Therefore, hold promise alternative means to inhibit biofilm formation industry sector.

Language: Английский

Citations

30