MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression

Neural Regeneration Research, Год журнала: 2019, Номер 15(4), С. 606 - 606

Опубликована: Окт. 18, 2019

Multiple sclerosis is a chronic autoimmune disease of the central nervous system. It main cause non-traumatic neurological disability in young adults. mostly affects people aged 20-50 years; however, it can occur children and much older Factors identified distribution MS include age, gender, genetics, environment, ethnic background. usually associated with progressive degrees disability. The involves demyelination axons system causes brain spinal cord neuronal loss atrophy. Diagnosing multiple based on patient's medical history including symptoms, physical examination, various tests such as magnetic resonance imaging, cerebrospinal fluid blood tests, electrophysiology. course not well correlated biomarkers presently used clinical practice. Blood-derived that detect distinguish different phenotypes may be advantageous personalized treatment disease-modifying drugs to predict response treatment. studies reviewed have shown expression levels large number miRNAs peripheral blood, serum, exosomes isolated from are altered each other. Further warranted independently validate these findings so individual or pairs serum potential diagnostic markers for adult pediatric monitoring progression therapy.

Язык: Английский

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miR-155 as an Important Regulator of Multiple Sclerosis Pathogenesis. A Review

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(9), С. 4332 - 4332

Опубликована: Апрель 21, 2021

Multiple sclerosis (MS) is a chronic, immune-mediated disease and the leading cause of disability among young adults. MicroRNAs (miRNAs) are involved in post-transcriptional regulation gene expression. Of them, miR-155 crucial regulator inflammation plays role modulating autoimmune response MS. blood-brain barrier (BBB) disruption via down-regulation key junctional proteins under inflammatory conditions. It drives demyelination processes by contributing to, e.g., microglial activation, polarization astrocytes, CD47 protein affecting transcription factors. has huge impact on development neuropathic pain indirectly influences regulatory T (Treg) cell differentiation alleviation hypersensitivity. This review also focused neuropsychiatric symptoms appearing as result disease-associated stressors, brain atrophy, pro-inflammatory Recent studies revealed regulating anxiety, stress, hippocampus, treatment-resistant depression. Inhibition expression was demonstrated to be effective preventing pathophysiology aimed support better understanding great dysregulation various aspects MS highlight future perspectives for this molecule.

Язык: Английский

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Exosome Content–Mediated Signaling Pathways in Multiple Sclerosis

Molecular Neurobiology, Год журнала: 2024, Номер 61(8), С. 5404 - 5417

Опубликована: Янв. 8, 2024

Язык: Английский

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Extracellular Vesicles in Neuroinflammation

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 8

Опубликована: Янв. 21, 2021

Extracellular vesicles (EVs) are a heterogenous group of membrane-bound particles that play pivotal role in cell–cell communication, not only participating many physiological processes, but also contributing to the pathogenesis several diseases. The term EVs defines and different based on their biogenesis release pathway, including exosomes, microvesicles (MVs), apoptotic bodies. However, classification, biological function as well protocols for isolation detection still under investigation. Recent evidences suggest existence novel subpopulations EVs, increasing degree heterogeneity between EV types subtypes. have been shown roles CNS biomarkers vehicles drugs other therapeutic molecules. They known cross blood brain barrier, allowing be detectable peripheral fluids, cargo may give information parental cells pathological process they involved in. In this review, we summarize knowledge multiple sclerosis (MS) discuss recent potential applications diagnostic targets.

Язык: Английский

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CNS endothelial derived extracellular vesicles are biomarkers of active disease in multiple sclerosis

Fluids and Barriers of the CNS, Год журнала: 2022, Номер 19(1)

Опубликована: Фев. 8, 2022

Abstract Background Multiple sclerosis (MS) is a complex, heterogenous disease characterized by inflammation, demyelination, and blood–brain barrier (BBB) permeability. Currently, active determined physician confirmed relapse or detection of contrast enhancing lesions via MRI indicative BBB However, clinical confirmation can be cumbersome. As such, monitoring in MS could benefit from identification an easily accessible biomarker disease. We believe extracellular vesicles (EV) isolated plasma are excellent candidates to fulfill this need. Because the critical role permeability plays pathogenesis disease, we sought identify EV originating central nervous system (CNS) endothelial as biomarkers MS. cells secrete more when stimulated injured, hypothesized that circulating concentrations CNS derived will increased patients with Methods To test this, developed novel method patient using flow cytometry. Endothelial were identified absence lymphocyte platelet markers CD3 CD41, respectively, positive expression pan-endothelial CD31, CD105, CD144. determine if originated cells, expressing CD144 evaluated for myelin protein MAL, specifically expressed compared peripheral organs. Results Quality control experiments indicate detected our cytometry 0.2 1 micron size. Flow analysis 20 healthy controls, 16 relapsing–remitting (RRMS) not receiving modifying therapy, 14 RRMS stable 17 relapsing-RRMS natalizumab, ocrelizumab revealed significant increase concentration all other groups ( p = 0.001). Conclusions: For first time, have circulation human blood samples. pilot study levels may

Язык: Английский

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Role of miR-155 in inflammatory autoimmune diseases: a comprehensive review

Inflammation Research, Год журнала: 2022, Номер 71(12), С. 1501 - 1517

Опубликована: Окт. 29, 2022

Язык: Английский

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Noncoding RNAs in multiple sclerosis

Clinical Epigenetics, Год журнала: 2018, Номер 10(1)

Опубликована: Ноя. 29, 2018

Multiple sclerosis (MS), a chronic inflammatory demyelinating disease of the central nervous system, is characterized by axonal degeneration and gliosis. Although causes MS remain unknown, gene dysregulation in system has been associated with pathogenesis. As such, various regulators expression may be contributing factors. The noncoding (nc) RNAs have piqued interest researchers due to their known functions human physiology pathological processes, despite being generally as transcripts without apparent protein-coding capacity. Accumulating evidence indicated that ncRNAs participate regulation acting epigenetic factors, especially long (l) micro (mi) RNAs, they are now recognized key regulatory molecules MS. In this review, we summarize most current studies on contribution pathogenic processes discuss potential applications diagnosis treatment

Язык: Английский

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Immune-related miRNA expression patterns in peripheral blood mononuclear cells differ in multiple sclerosis relapse and remission

Journal of Neuroimmunology, Год журнала: 2018, Номер 317, С. 67 - 76

Опубликована: Янв. 5, 2018

Язык: Английский

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The molecular mechanism underlying GABAergic dysfunction in nucleus accumbens of depression‐like behaviours in mice

Journal of Cellular and Molecular Medicine, Год журнала: 2019, Номер 23(10), С. 7021 - 7028

Опубликована: Авг. 20, 2019

Abstract Depression is the most frequent psychiatric disorder in world. Recent evidence has shown that stress‐induced GABAergic dysfunction nucleus accumbens (NAc) contributed to pathophysiology of depression. However, molecular mechanisms underlying these pathological changes remain unclear. In this study, mice were constantly treated with chronic unpredictable mild stress (CUMS) till showing depression‐like behaviours expression. GABA synthesis, release and uptake NAc tissue assessed by analysing expression level genes proteins Gad‐1, VGAT GAT‐3 qRT‐PCR Western blotting. The miRNA/mRNA network regulating was constructed based on bioinformatics prediction software further validated dual‐luciferase reporter assay vitro vivo, respectively. Our results showed GAT‐3, Gad‐1 mRNA protein significantly decreased from CUMS‐induced than control mice. miRNA‐144‐3p, miRNA‐879‐5p, miR‐15b‐5p miRNA‐582‐5p directly down‐regulated increased. mRNA/miRNA regulatory network, regulated binding seed sequence miR‐144‐3p, miR‐15b‐5p, miR‐879‐5p could be served negative post‐regulators different sites 3′‐UTR. Chronic causes impaired up‐regulating miRNAs down‐regulating mRNAs proteins, which may reveal for concentrations

Язык: Английский

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Environmental Influencers, MicroRNA, and Multiple Sclerosis

Journal of Central Nervous System Disease, Год журнала: 2020, Номер 12, С. 117957351989495 - 117957351989495

Опубликована: Янв. 1, 2020

Multiple sclerosis (MS) is a complex neurological disorder characterized by an aberrant immune system that affects patients’ quality of life. Several environmental factors have previously been proposed to associate with MS pathophysiology, including vitamin D deficiency, Epstein-Barr virus (EBV) infection, and cigarette smoking. These may influence cellular molecularity, interfering proliferation, differentiation, apoptosis. This review argues small noncoding RNA named microRNA (miRNA) influences these factors’ mode action. Dysregulation in the miRNAs network deeply impact hemostasis, thereby possibly resulting pathogenicity. article represents literature author’s theory how induce dysregulations network, which could ultimately affect

Язык: Английский

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