Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 14, 2023
Abstract
Objective
:
To
explore
the
neuroimaging
characteristics
of
eczema-induced
pruritus
with
resting-state
functional
Magnetic
Resonance
Imaging
(rs-fMRI).
Methods
42
patients
eczema
were
recruited
in
PE
group,
and
healthy
participants
included
HC
group.
The
Visual
Analogue
Score
(VAS),
12-Item
Pruritus
Severity
Scale
(12-PSS),
Pittsburgh
Sleep
Quality
Index
(PSQI)
Self-rating
Anxiety
(SAS)
recorded
different
values
fraction
Amplitude
Low
Frequency
Fluctuation
(fALFF)
connectivity(FC)
compared
after
rs-fMRI
scanning.
Results:
Compared
fALFF
left
precentral
gyrus,
postcentral
supplementary
motor
area
(SMA)
midcingulate
cortex
group
increased.
FC
between
gyrus
bilateral
superior
temporal
hippocampus,
inferior
occipital
decreased.
SMA
12-PSS
score
was
positively
correlated
value
gyrus.
Conclusion:
caused
increased
spontaneous
activity
given
cerebral
regions,
involving
perception
itch,
control
scratching
movements,
expression
itch-related
emotions.
Simultaneously,
aberrant
result
corresponding
alterations
networks,
primarily
characterized
by
a
decrease
symmetry
such
as
hippocampus.
Meanwhile,
there
is
correlation
regions
clinical
scales,
which
provided
potential
neurobiological
markers
for
future
study
pruritus.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Март 8, 2024
Abstract
Pain
and
itch
are
recognized
as
antagonistically
regulated
sensations;
pain
suppresses
itch,
whilst
inhibition
enhances
itch.
The
neural
mechanisms
at
the
central
nervous
system
(CNS)
underlying
these
pain-itch
interactions
still
need
to
be
explored.
Here,
we
revealed
contrasting
role
of
orexin-producing
neurons
(ORX
neurons)
in
lateral
hypothalamus
(LH),
which
while
enhancing
processing,
by
applying
optogenetics
acute
pruritus
model.
We
also
that
circuit
ORX
from
LH
periaqueductal
gray
regions
served
modulation
processing
using
optogenetic
terminal
techniques.
Additionally,
an
atopic
dermatitis
model,
confirmed
involvement
regulating
chronic
could
lead
a
novel
therapeutic
target
for
persistent
clinical
settings.
Our
findings
provide
new
insight
into
mechanism
antagonistic
regulation
between
CNS.
Irritable
bowel
syndrome
(IBS)
is
a
prevalent
functional
gastrointestinal
disease
characterized
by
chronic
visceral
pain
with
complex
etiology
and
challenging
treatment.
Although
accumulating
evidence
supports
the
involvement
of
central
nervous
system
sensitization
in
development
pain,
precise
molecular
mechanisms
remain
incompletely
understood.
In
this
study,
we
highlight
critical
regulatory
role
lysine-specific
demethylase
6B
(KDM6B)
anterior
cingulate
cortex
(ACC)
pain.
To
simulate
clinical
IBS
conditions,
utilized
neonatal
maternal
deprivation
(NMD)
mouse
model.
Our
results
demonstrated
that
NMD
induced
anxiety-like
behaviors
mice.
Notably,
protein
expression
level
KDM6B
significantly
increased
ACC
mice,
leading
to
reduction
H32K7me3.
Immunofluorescence
staining
revealed
primarily
co-localizes
neurons
ACC,
minimal
presence
microglia
astrocytes.
Injecting
GSK-J4
(a
KDM6B-specific
inhibitor)
into
resulted
significant
alleviation
behaviors,
as
well
remarkable
NR2B
level.
ChIP
assay
further
indicated
regulates
influencing
demethylation
H3K27me3.
summary,
our
findings
underscore
regulating
These
insights
provide
basis
for
understanding
pathways
involved
may
pave
way
targeted
therapeutic
interventions.
Brain and Behavior,
Год журнала:
2025,
Номер
15(3)
Опубликована: Март 1, 2025
To
explore
the
neuroimaging
characteristics
of
eczema-induced
pruritus
with
resting-state
functional
magnetic
resonance
imaging
(rs-fMRI).
A
total
42
patients
eczema
were
recruited
in
PE
group,
and
healthy
participants
included
HC
group.
The
Visual
Analogue
Score
(VAS),
12-Item
Pruritus
Severity
Scale
(12-PSS),
Pittsburgh
Sleep
Quality
Index
(PSQI),
Self-Rating
Anxiety
(SAS)
recorded
different
values
fraction
amplitude
low-frequency
fluctuation
(fALFF)
connectivity
(FC)
compared
after
rs-fMRI
scanning.
Compared
fALFF
left
precentral
gyrus,
postcentral
supplementary
motor
area
(SMA),
midcingulate
cortex
group
increased.
FC
between
bilateral
superior
temporal
hippocampus,
inferior
occipital
gyrus
decreased.
SMA
12-PSS
score
was
positively
correlated
value
gyrus.
caused
increased
spontaneous
activity
given
cerebral
regions,
involving
perception
itch,
control
scratching
movements,
expression
itch-related
emotions.
Meanwhile,
there
is
a
correlation
regions
clinical
scales,
which
provided
potential
neurobiological
markers
for
future
study
pruritus.
Journal of Neuroscience,
Год журнала:
2023,
Номер
43(8), С. 1334 - 1347
Опубликована: Янв. 18, 2023
Itch
is
an
uncomfortable
and
complex
sensation
that
elicits
the
desire
to
scratch.
The
nucleus
accumbens
(NAc)
activity
important
in
driving
sensation,
motivation,
emotion.
Excitatory
afferents
from
medial
prefrontal
cortex
(mPFC),
amygdala,
hippocampus
are
crucial
tuning
of
dopamine
receptor
D1-expressing
D2-expressing
medium
spiny
neurons
(Drd1-MSN
Drd2-MSN)
NAc.
However,
a
cell-type
neural
circuity-based
mechanism
NAc
underlying
acute
itch
remains
unclear.
We
found
induced
by
compound
48/80
(C48/80)
decreased
intrinsic
membrane
excitability
Drd1-MSNs,
but
not
Drd2-MSNs,
core
male
mice.
Chemogenetic
activation
Drd1-MSNs
alleviated
C48/80-induced
scratching
behaviors
itch-related
anxiety-like
behaviors.
In
addition,
C48/80
enhanced
frequency
spontaneous
EPSCs
(sEPSCs)
reduced
paired-pulse
ratio
(PPR)
electrical
stimulation-evoked
Drd1-MSNs.
Furthermore,
increased
excitatory
synaptic
mPFC,
basolateral
amygdala
(BLA)
or
ventral
(vHipp).
Consistently,
mPFC-NAc
projecting
pyramidal
was
after
treatment.
inhibition
relieved
Moreover,
pharmacological
κ
opioid
(KOR)
suppressed
behaviors,
modulation
KOR
resulted
changes
presynaptic
inputs
C48/80-treated
Together,
these
results
reveal
plasticity
synapses
mPFC
indicate
modulatory
role
behaviors.SIGNIFICANCE
STATEMENT
stimuli
cause
strongly
anxiety
patients.
related
mechanisms
remain
largely
present
study,
we
demonstrated
pruritogen
shapes
(Drd1-MSNs)
glutamatergic
(mPFC)
neurons.
relieves
alleviates
itch.
Thus,
targeting
may
provide
effective
treatments
for
Chronic
itch
often
clinically
coexists
with
anxiety
symptoms,
creating
a
vicious
cycle
of
itch-anxiety
comorbidities
that
are
difficult
to
treat.
However,
the
neuronal
circuit
mechanisms
underlying
comorbidity
in
chronic
remain
elusive.
Here,
we
report
anxiety-like
behaviors
mouse
models
and
identify
γ-aminobutyric
acid–releasing
(GABAergic)
neurons
lateral
septum
(LS)
as
key
player
itch–induced
anxiety.
In
addition,
is
accompanied
enhanced
activity
synaptic
plasticity
excitatory
projections
from
thalamic
nucleus
reuniens
(Re)
onto
LS
GABAergic
neurons.
Selective
chemogenetic
inhibition
Re
→
notably
alleviated
anxiety,
no
impact
on
induced
by
restraint
stress.
Last,
hypothalamus
(LH)
receive
monosynaptic
mediate
These
findings
underscore
potential
significance
LH
pathway
regulating
comorbid
symptoms
associated
itch.
