Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104882 - 104882

Опубликована: Янв. 1, 2025

Язык: Английский

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Practical diagnostic algorithms for Chagas disease: a focus on low resource settings

Expert Review of Anti-infective Therapy, Год журнала: 2023, Номер 21(12), С. 1287 - 1299

Опубликована: Ноя. 7, 2023

Introduction Chagas disease, caused by parasite Trypanosoma cruzi, is the most important neglected tropical disease in Americas. Two drugs are available for treatment, but access to them challenging, part due complex diagnostic algorithms. These stage-dependent, involve multiple tests, and ill-adapted reality of vast areas where endemic. Molecular serologic tools used detect acute chronic infections, with performance latter showing geographic differences. Breakthroughs development new include validation a loop-mediated isothermal amplification assay infections (T. cruzi-LAMP), regional several rapid tests (RDTs) infection, which simplify testing resource-limited settings. The literature search was carried out MEDLINE database until 1 August 2023.

Язык: Английский

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Nanopore sequencing of infectious fluid is a promising supplement for gold-standard culture in real-world clinical scenario

Frontiers in Cellular and Infection Microbiology, Год журнала: 2024, Номер 14

Опубликована: Янв. 30, 2024

Introduction Infectious diseases are major causes of morbidity and mortality worldwide, necessitating the rapid identification accurate diagnosis pathogens. While unbiased metagenomic next-generation sequencing (mNGS) has been extensively utilized in clinical pathogen scientific microbiome detection, there is limited research about application nanopore platform-based mNGS diagnostic performance various infectious fluid samples. Methods In this study, we collected 297 suspected fluids from 10 centers detected them with conventional microbiology culture platform–based mNGS. The objective was to assess detective nanopore-sequencing technology (NST) real-world scenarios. Results Combined gold-standard adjudication, demonstrated nearly 100% positive predictive agreements microbial-colonized sites, such as respiratory urinary tracts. For samples initially sterile body microorganisms were highly pathogens, negative relatively higher than those particularly abscess 95.7% cerebrospinal fluid. Furthermore, consistent also observed antimicrobial resistance genes drug susceptibility testing pathogenic strains Escherichia coli , Staphylococcus aureus Acinetobacter baumannii . Discussion Rapid NST a promising tool supplement culture, it potential improve patient prognosis facilitate treatment diseases.

Язык: Английский

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Detection of monkeypox virus based on a convenient and sensitive single-step RPA-CRISPR/Cas12a strategy

RSC Advances, Год журнала: 2024, Номер 14(21), С. 14775 - 14783

Опубликована: Янв. 1, 2024

The global outbreak of monkeypox virus (MPXV) has highlighted the need for rapid molecular diagnostics techniques. In this study, a single-step recombinase polymerase amplification (RPA)-CRISPR/Cas12a system was developed and sensitive detection MPXV. limit assay 1 copy per μL extracted nucleic acids. A heating lysis method integrated to further simplify sample processing workflow shorten time 40 min from result. reaction mixture can be lyophilized improve its accessibility in resource-limited settings. analysis results proposed RPA-CRISPR/Cas12a clinical MPXV positive negative samples were 100% consistent with standard PCR assay. These demonstrate feasibility efficiency accurate real-world settings, showcasing potential utility urgent practical

Язык: Английский

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Amplifying mutational profiling of extracellular vesicle mRNA with SCOPE

Nature Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 7, 2024

Язык: Английский

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Phosphoramidate Azole Oligonucleotides for Single Nucleotide Polymorphism Detection by PCR

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(1), С. 617 - 617

Опубликована: Янв. 3, 2024

Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for treatment various malignancies. The most common KRAS-activating mutations are caused by single-nucleotide mutations, which usually determined using PCR, allele-specific DNA primers. Oligonucleotide primers with uncharged or partially charged internucleotide phosphate modification have proved their ability to increase sensitivity and specificity single nucleotide mutation detection. To enhance detection, novel oligonucleotides four types phosphates modification, phosphoramide benzoazole (PABA) (PABAO), was used prove concept on KRAS model. molecular effects different site-specific PABA in a primer template synthesis full-length elongation product PCR efficiency were evaluated. (AS-PCR) plasmid templates showed significant analysis without changes Cq values compared unmodified primer. universal mismatch-like disturbance, can be polymorphism discrimination applications. insights affect structural features PABAO connection AS-PCR results, improvements support further design platforms biological targets testing.

Язык: Английский

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Progress in quantum dot-based biosensors for microRNA assay: A review

Analytica Chimica Acta, Год журнала: 2023, Номер 1278, С. 341615 - 341615

Опубликована: Июль 13, 2023

Язык: Английский

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CRISPR-Cas based diagnostic tools: Bringing diagnosis out of labs

Diagnostic Microbiology and Infectious Disease, Год журнала: 2024, Номер 109(2), С. 116252 - 116252

Опубликована: Март 8, 2024

Язык: Английский

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A Single Electrochemical Biosensor Designed to Detect Any Virus

Analytical Chemistry, Год журнала: 2024, Номер 96(15), С. 5752 - 5756

Опубликована: Апрель 1, 2024

Viruses are the primary cause of many infectious diseases in both humans and animals. Various testing methods require an amplification step viral RNA sample before detection, with quantitative reverse transcription polymerase chain reaction (RT-qPCR) being one most widely used along lesser-known like Nucleic Acid Sequence-Based Amplification (NASBA). NASBA offers several advantages, such as isothermal high selectivity for specific sequences, making it attractive option low-income facilities. In this research, we employed a single electrochemical biosensor (E-Biosensor) designed potentially detecting any virus by modifying protocol. modified protocol, primer is additional 22-nucleotide sequence (tag region) at 5′-end, which added to process. This tag region becomes part final amplicon generated NASBA. It can hybridize E-Biosensor probe set, enabling subsequent detection. Using approach, successfully detected three different viruses design, demonstrating platform's potential

Язык: Английский

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Development of a duplex recombinase polymerase amplification-lateral flow strip assay with 1 min of DNA extraction for simultaneous identification of pork and chicken ingredients

Journal of Food Composition and Analysis, Год журнала: 2024, Номер 132, С. 106253 - 106253

Опубликована: Апрель 23, 2024

Язык: Английский

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