Untrimmed ITS2 metabarcode sequences cause artificially reduced abundances of specific fungal taxa DOI Creative Commons
Kathleen E. Kyle, Jonathan L. Klassen

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 3, 2024

Abstract Advances in DNA metabarcoding have greatly expanded our knowledge of microbial communities recent years. Pipelines and parameters been tested extensively for bacterial using the 16S rRNA gene best practices are largely established. For fungal ITS gene, however, only a few studies considered how such pipelines can affect community prediction. Here we report novel bias uncovered during ITS2 sequencing Trichoderma -infected ant fungus gardens confirmed mock communities. Abnormally low forward read quality caused reads to be computationally filtered before pair merging, thus almost entirely eliminating ASVs from resulting profiles. Sliding window trimming filtering allowed most these pass merge successfully, producing profiles that now correlated with visual signs infection matched composition Applying sliding previously generated environmental dataset increased detected diversity again overcame biases against instead detect it nearly every sample often at high relative abundances. This analysis additionally identified similar, but distinct, second genus Meyerozyma . The prevalence other sequences is unknown may widespread. We therefore advocate routine use as practice analysis. Importance Metabarcode produces abundance presumed reflect actual samples they analyze. However, this assumption not always tested, taxon-specific apparent, especially low-abundance taxa complex aberrations dramatic reductions abundances specific multiple datasets characterizing gardens. Such widespread environments taxa, thereby causing incorrect descriptions mycobiomes.

Язык: Английский

New Aquilariomyces and Mangifericomes species (Pleosporales, Ascomycota) from Aquilaria spp. in China DOI Creative Commons
Tian‐Ye Du, Samantha C. Karunarathna, Kevin D. Hyde

и другие.

MycoKeys, Год журнала: 2025, Номер 112, С. 103 - 125

Опубликована: Янв. 13, 2025

Saprobic fungi are known for their critical role in decomposition and nutrient cycling. The study of saprobic is equally important, as it helps understanding ecological roles identifying hidden diversity. This focused on Aquilaria , which poorly studied compared to economically important hosts like coffee, tea, rubber. Our rigorous process led the collection two new terrestrial from Guangdong Yunnan provinces China. After extensive phylogenetic analyses detailed comparison morphological characteristics, collections were identified species belonging Pleosporales, Ascomycota. Aquilariomyces maomingensis sp. nov. was isolated sinensis Province, while Mangifericomes aquilariae Province. Full descriptions, photo plates, (maximum likelihood Bayesian inference based LSU, ITS, SSU, tef 1-α, rpb 2 gene combinations) provided, along with a comprehensive list associated spp.

Язык: Английский

Процитировано

0
Two novel hyphomycetes associated with ferns from China DOI Creative Commons
Jing-Yi Zhang, Kevin D. Hyde, Lijuan Zhang

и другие.

MycoKeys, Год журнала: 2025, Номер 113, С. 101 - 121

Опубликована: Янв. 31, 2025

During an ongoing investigation of fungi associated with ferns in southwestern China, three hyphomycetes were discovered on the dead rachises Angiopterisfokiensis and unidentified fern. Based morphology multi-gene phylogenetic analyses, Arthrobotrysangiopteridis Corynesporaseptata are introduced as new species. is a nematode-trapping fungus characterized by macronematous, mononematous, hyaline conidiophores, conidiogenous cells polyblastic denticles at each node, 0-1-septate, clavate to elongate pyriform, conidia. features pale brown dark integrated, monotretic up 7-distoseptate one true septum, subcylindrical obclavate, Detailed descriptions illustrations these two species provided, along morphological comparisons taxa closely related

Язык: Английский

Процитировано

0
Novel Strategies for Preventing Fungal Infections—Outline DOI Creative Commons

Damilola Janet Agbadamashi,

Claire Price

Pathogens, Год журнала: 2025, Номер 14(2), С. 126 - 126

Опубликована: Фев. 1, 2025

Fungal infections are a significant global health challenge, causing approximately 3.8 million deaths annually, with immunocompromised populations particularly at risk. Traditional antifungal therapies, including azoles, echinocandins, and polyenes, face limitations due to rising resistance, toxicity, inadequate treatment options. This review explores innovative strategies for preventing managing fungal infections, such as vaccines, peptides, nanotechnology, probiotics, immunotherapy. Vaccines offer promising avenues long-term protection, despite difficulties in their development complexity immune evasion mechanisms. Antifungal peptides provide novel class of agents broad-spectrum activity reduced resistance risk, whilst nanotechnology enables targeted, effective drug delivery systems. Probiotics show potential vulvovaginal candidiasis, by maintaining microbial balance. Immunotherapy leverages system modulation enhance defenses, omics technologies deliver comprehensive insights into biology, paving the way therapeutic vaccine targets. While these approaches hold immense promise, challenges cost, accessibility, translational barriers remain. A coordinated effort among researchers, clinicians, policymakers is critical advancing addressing burden effectively.

Язык: Английский

Процитировано

0
Morphological characteristics and phylogenetic analyses revealed four new species (Basidiomycota) in the Yunnan-Guizhou Plateau, China DOI Creative Commons

Siyuan He,

Lu Wang, Kaize Shen

и другие.

MycoKeys, Год журнала: 2025, Номер 113, С. 237 - 262

Опубликована: Фев. 12, 2025

Four new fungi, viz. Clavulinopsiswumengshanensis (Clavariaceae, Agaricales), Henningsomycesbambusae (Porotheleaceae, Xenasmabisterigmatae, and X.guttulata (Xenasmataceae, Russulales), from Yunnan Province in China, are proposed, based on a combination of morphological features molecular evidence. Phylogenetic analyses were conducted using combined dataset internal transcribed space nuclear ribosomal RNA large subunit sequences. The ITS+LSU analysis showed that sp. nov. groups with C.aurantiocinnabarina, forms sister group H.candidus, Xenasmabisterigmatae is clustered X.rimicola, X.pruinosum. morphology multi-gene phylogenetic confirmed the placement four taxa. distinguished by buff-yellow to straw-yellow basidiomata, clavate subcylindrical basidia sterigmata, subglobose basidiospores several guttules (7-8.5 × 6-7.5 µm); characterized white cream basidiomata short cylindric tubular colonies, cylindrical two sterigmata globose (6.5-8.5 6.5-8.5 can be its membranaceous ash-grey ellipsoid (10-12.5 8-10.5 narrowly (7-9 5.5-7.5 µm).

Язык: Английский

Процитировано

0
Morphological characteristics and phylogenetic analyses revealed five new species (Basidiomycota) from Southwestern China DOI Creative Commons

AiGe Yang,

Lu Wang, Yongjun Hu

и другие.

MycoKeys, Год журнала: 2025, Номер 114, С. 177 - 212

Опубликована: Фев. 28, 2025

In the present study, five new wood-inhabiting fungal species, Conferticiumalbocremeum (Stereaceae, Russulales), Dendrocorticiopsisluteoalba (Punctulariaceae, Corticiales), Eichleriellabiluoxueshanensis (Auriculariaceae, Auriculariales), Gloeohypochniciumyunnanense (Incertae sedis, and Punctularianigrodontea collected from southern China, are proposed based on a combination of morphological features phylogenetic evidence. is characterized by membranaceous smooth basidiomata with white to cream surface ellipsoid verrucose basidiospores (9-11 × 5-7 µm); buff, ellipsoid, thin-walled (7-8 4.5-5.5 coriaceous grandinioid buff cinnamon-buff hymenophore allantoid, (13.5-17.5 7-9 cream, globose, thick-walled warted (10-12 10-11.5 µm), resupinate effused-reflexed (8.5-10 5-6 µm). Sequences internal transcribed spacers (ITS) large subunit (nLSU) nuclear ribosomal DNA (rDNA) markers studied samples were generated. Phylogenetic analyses performed ITS+nLSUrDNA gene regions maximum likelihood, parsimony, Bayesian inference methods revealed that species belong genera Conferticium, Dendrocorticiopsis, Eichleriella, Gloeohypochnicium, Punctularia. Descriptions, illustrations, analysis results, comparison closely related taxa provided.

Язык: Английский

Процитировано

0
Fungi: Pioneers of chemical creativity – Techniques and strategies to uncover fungal chemistry DOI Creative Commons
Hedda Schrey, Christopher Lambert, Marc Stadler

и другие.

IMA Fungus, Год журнала: 2025, Номер 16

Опубликована: Март 7, 2025

Natural product discovery from fungi for drug development and description of novel chemistry has been a tremendous success. This success is expected to accelerate even further, owing the advent sophisticated technical advances that recently led an unparalleled biodiversity in fungal kingdom. review aims give overview on i) important secondary metabolite-derived drugs or leads, ii) discuss analytical strategic framework how natural lead identification transformed earlier days present, iii) knowledge biology facilitates new compounds, iv) point out endeavors understanding metabolite order systematically explore genomes by utilizing synthetic biology. An outlook given, underlining necessity collaborative cooperative scenario harness full potential metabolome.

Язык: Английский

Процитировано

0
Introducing Melanocucurbitaria uktampratovii sp. nov. and the sexual morph of Melanocamarosporioides ugamica in Melanommataceae (Dothideomycetes, Pleosporales) DOI Creative Commons
Dhandevi Pem, Rajesh Jeewon, Yusufjon Gafforov

и другие.

MycoKeys, Год журнала: 2025, Номер 115, С. 19 - 42

Опубликована: Март 7, 2025

Most species of Melanommataceae are saprobic on decayed parts various plants in tropical and temperate terrestrial habitats. During a survey microfungi associated with Uzbekistan, two melanommataceous taxa were collected from dead branches Rosa ecae (Rosaceae) Salvia karelinii (Lamiaceae). This study introduces new species, Melanocucurbitaria uktampratovii , provides host sexual morph record for Melanocamarosporioides ugamica based morphological observations multi-gene phylogenetic analyses concatenated LSU, SSU, ITS, TEF-1 sequence data. is the second described within this genus differs type having smaller ascomata, asci, ascospores, different number septa. The characterized by globose to ovoid cylindrical-clavate ellipsoidal, muriform ascospores 5–7 transversely septate, 5–11 vertical Illustrations descriptions provided, along ecological comparisons similar their respective genera.

Язык: Английский

Процитировано

0
Punctochaete murina gen. et sp. nov. (Agaricomycetes, Basidiomycota) from southwestern China DOI Creative Commons
Junhong Dong, Qi Li, JIANGQING SU

и другие.

European Journal of Taxonomy, Год журнала: 2025, Номер 981, С. 96 - 113

Опубликована: Март 12, 2025

Wood-inhabiting fungi play an integral role in wood degradation and the cycle of matter ecological system. They are considered a ʻkey playerʼ decomposition because their ability to produce all kinds enzymes that break down lignin, cellulose hemicellulose. In this study, some wood-inhabiting mushrooms were collected from Yunnan-Guizhou Plateau, Asia, based on morphological molecular evidence, they introduced as Punctochaete murina J.H.Dong & C.L.Zhao gen. et sp. nov. is characterized by resupinate basidiomata with punctate hymenial surface, monomitic hyphal system generative hyphae bearing clamp connections, cylindrical allantoid, thin-walled, smooth, slightly curved basidiospores. Sequences internal transcribed spacer (ITS) large subunit (nLSU) markers studied samples generated, phylogenetic analyses performed maximum likelihood, parsimony Bayesian inference methods. Phylogenetic ITS+nLSU nuclear RNA gene regions showed forms monophyletic lineage within order Auriculariales which it sister clade comprising two closely related genera: Exidiopsis Tremellochaete.

Язык: Английский

Процитировано

0
Airborne fungal spore concentrations double but diversity decreases with warmer winter temperatures in the Brazilian Atlantic Forest biodiversity hotspot DOI Creative Commons
Maurício Cruz Mantoani, Camila Sapucci, Lara Chaves Carvalho Guerra

и другие.

The Microbe, Год журнала: 2025, Номер unknown, С. 100300 - 100300

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Additions to the coriaceous families Peniophoraceae and Stereaceae (Russulales): Six novel wood-inhabiting taxa in the genera Conferticium, Gloeocystidiellum, and Peniophora from southwest China DOI Creative Commons
Lu Wang,

Yonggao Zhu,

Siyuan He

и другие.

MycoKeys, Год журнала: 2025, Номер 115, С. 273 - 308

Опубликована: Март 21, 2025

Russulales comprises a highly diverse group of species with respect to basidiomata morphology and hymenophore configuration, in which this order is heterogeneous, that can be classified as resupinate, effused-reflexed, discoid, clavarioid, pileate, or stipitate, varied hymenophores such smooth, hydnoid, poroid, lamellate different russuloid species. Species have been considered significant economic value. Six new wood-inhabiting fungi belonging the genera Conferticium , Gloeocystidiellum Peniophora two families, Peniophoraceae Stereaceae (Russulales), were found southwest China. Sequences ITS+nLSU loci six taxa generated, phylogenetic analyses performed maximum likelihood, parsimony, Bayesian inference methods an emphasis on phylogeny smooth order. The combined analysis showed grouped within Russulales, tuberculatum cremeum G. fissuratum into family Stereaceae, albohymenia P. hengduanensis punctata Peniophoraceae. multi-gene confirmed novelty placement taxa. Descriptions, illustrations, results are provided.

Язык: Английский

Процитировано

0