Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101715 - 101715
Опубликована: Март 1, 2025
Язык: Английский
Indian Journal of Microbiology, Год журнала: 2024, Номер 64(1), С. 100 - 109
Опубликована: Янв. 3, 2024
Язык: Английский
Процитировано
5
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5543 - 5543
Опубликована: Май 19, 2024
Milk is a fundamental component of the human diet, owing to its substantial nutritional content. In addition, milk contains nanoparticles called extracellular vesicles (EVs), which have indicated their potential beneficial roles such as cell-to-cell communication, disease biomarkers, and therapeutics agents. Amidst other types EVs, EVs (MEVs) significance due high abundance, easy access, stability in harsh environmental conditions, low pH gut. There been plenty studies conducted evaluate therapeutic bovine MEVs over past few years, attention has given engineering for drug delivery targeted therapy. However, there gap between experimental findings available clinical trials many challenges related EV isolation, cargo, uniformity material. This review aims provide comprehensive comparison various techniques isolation offers summary described last decade, analyzing further applications. Although number aspects still need be elucidated, data point role candidate with potential, supplementation would pave way understanding in-depth effects.
Язык: Английский
Процитировано
5
International Wound Journal, Год журнала: 2024, Номер 21(4)
Опубликована: Март 26, 2024
Abstract Hyperbaric oxygen therapy (HBOT) has been used in patients with diabetic foot ulcers (DFU) for many years, but its clinical efficacy is still controversial. Therefore, this study explored the of HBOT applied to DFU by means meta‐analysis. PubMed, Cochrane Library, Embase, CNKI and Wanfang databases were searched, from database inception October 2023, published randomised controlled trials (RCTs) collected. Two investigators independently screened collected literature, extracted relevant data assessed quality literature. Review Manager 5.4 software was analysis. Twenty‐nine RCTs 1764 included. According combined results, when compared conventional treatment, significantly increased complete healing rate DFUs (46.76% vs. 24.46%, odds ratio [OR]: 2.83, 95% CI: 2.29–3.51, p < 0.00001) decreased amputation (26.03% 45.00%, OR: 0.41, 0.18–0.95, = 0.04), incidence adverse events higher (17.37% 8.27%, 2.49, 1.35–4.57, 0.003), whereas there no significant difference mortality (6.96% 12.71%, 0.52, 0.21–1.28, 0.16). Our results suggest that effective increasing decreasing DFUs, increases events, while it effect on mortality.
Язык: Английский
Процитировано
4
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(15), С. 8304 - 8331
Опубликована: Апрель 8, 2024
Extracellular vesicles (EVs) are nano to-micrometer-sized sacs that released by almost all animal and plant cells act as intercellular communicators transferring their cargos between the source target cells. As a safe scalable alternative to conditioned medium-derived EVs, milk-derived EVs (miEVs) have recently gained great deal of popularity. Numerous studies shown miEVs intrinsic therapeutic actions can treat diseases enhance human health. Additionally, they be used natural drug carriers novel classes biomarkers. However, due complexity milk, successful translation from benchtop bedside still faces several unfilled gaps, especially lack standardized protocols for isolation high-purity miEVs. In this work, comprehensively reviewing bovine studies, we provide an overview current knowledge research on while highlighting challenges enormous promise class theranostics. It is hoped study will pave way clinical applications addressing opportunities.
Язык: Английский
Процитировано
4
Pathology - Research and Practice, Год журнала: 2024, Номер 259, С. 155388 - 155388
Опубликована: Июнь 5, 2024
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 382 - 382
Опубликована: Янв. 4, 2025
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and devastating lung disorder. In response to transforming growth factor-β (TGF-β), normal cells proliferate differentiate into myofibroblasts, which are instrumental in promoting disease progression. Small interfering RNA (siRNA) targeting heat shock protein 47 (HSP47) has been demonstrated alleviate IPF by blocking collagen synthesis secretion. Exosomes (EXOs) have investigated for drug delivery due their superior carrier properties. However, loading efficiency limiting factor widely application as carriers. this study, an ultrasonic microfluidic method was employed enhance the of siHSP47 EXOs, achieving 31.1% rate. EXOs were isolated from human embryonic kidney (293F) loaded with (EXO-siHSP47). The findings indicated that EXO-siHSP47 penetrated barrier effectively silenced HSP47 expression activated fibroblasts vitro. Western blotting immunofluorescence analyses confirmed significantly reduced secretion deposition extracellular matrix (ECM) proteins. Wound healing Transwell migration assays inhibited fibroblast differentiation migration. conclusion, 293F-derived present promising therapeutic strategy IPF.
Язык: Английский
Процитировано
0
Bioengineering & Translational Medicine, Год журнала: 2025, Номер unknown
Опубликована: Янв. 23, 2025
Abstract Mammalian milk, a multifaceted developmental biofluid, has attracted new attention due to its diverse constituents and their implications for health disease. Among these constituents, extracellular vesicles (EVs) have emerged as focal points of investigation. EVs, including exosomes small demonstrated biological activity in preclinical studies—including reports enhancement cognition neural complexity, promotion gastrointestinal development, barrier function microbiome richness, the bolstering immune response, facilitation musculoskeletal maturation neonates. The richness milk source EVs is noteworthy, with hundreds milliliters (at >10 12 EVs/mL) nanovesicles extractable from single liter (>10 14 EVs/starting milk). Techniques such tangential flow filtration hold promise scalable production, potentially extending thousands liters. Together scale increasing sophistication dairy industry, abundance underscores commercial potential various nutraceutical applications. Beyond natural bioactivity, (mEVs) present intriguing possibilities orally deliverable, non‐immunogenic pharmaceutical carriers, burgeoning interest utilization heart disease cancer chemotherapy vectors gene‐editing modules CrispR. This review synthesizes current knowledge on mEV biogenesis, characterization, isolation methodologies, cargo contents. Moreover, it delves into therapeutic mEVs, both inherently bioactive versatile platforms drug delivery. As efforts progress toward large‐scale implementation, rigorous safe, industrial‐scale production robust assay development will be pivotal harnessing translational milk.
Язык: Английский
Процитировано
0
Burns & Trauma, Год журнала: 2025, Номер 13
Опубликована: Янв. 1, 2025
Poor wound healing is a refractory process that places an enormous medical and financial burden on diabetic patients. Exosomes have recently been recognized as crucial players in the of lesions. They excellent stability, homing effects, biocompatibility, reduced immunogenicity novel cell-free therapies. In addition to transporting cargos target cells enhance intercellular communication, exosomes are beneficial nearly every phase healing. participate modulating inflammatory response, accelerating proliferation reepithelization, increasing angiogenesis, regulating extracellular matrix remodeling. Accumulating evidence indicates hydrogels or dressings conjunction with can prolong duration exosome residency wounds. This review provides overview mechanisms, delivery, clinical application, engineering, existing challenges use repair. We also propose future directions for biomaterials incorporating exosomes: 2D 3D scaffolds, loaded healing-promoting gases, intelligent biomaterials, prospect systematic application exosomes. These findings may might shed light treatments enlighten some studies improve quality life among diabetes
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 439 - 458
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Canadian Prosthetics & Orthotics Journal, Год журнала: 2025, Номер 8(1)
Опубликована: Фев. 21, 2025
BACKGROUND: Poor post-amputation healing delays prosthetic fitting, adversely affecting mortality, quality of life, and cardiovascular health. Current residual limb assessments are subjective lack standardized guidelines, emphasizing the need for objective biomarkers to improve prosthesis readiness assessments. OBJECTIVE(S): This review aimed identify predictive, diagnostic, indicative chemical tissues structures found in limbs adults with amputation. METHODOLOGY: scoping followed Joanna Briggs Institute (JBI) PRISMA-ScR guidelines. Searches using terms “biomarkers,” “wound healing,” “amputation” were performed across Web Science, Ovid Medline, Embase, Scopus, Cochrane, PubMed, CINAHL databases. Inclusion criteria were: 1) References healing; 2) Residuum tissue 3) Repeatable methodology ethical approval. Included articles evaluated evidence (QualSyst tool) level (JBI classification). Sources categorized by study (e.g., randomized controlled trial or bench research), wound (diabetic, amputation, other), model (human, murine, other) type. Chemical repeated categories, quantification methods reported on. FINDINGS: From 3,306 titles abstracts screened, 646 underwent full-text review, 203 met data extraction, 76% classified as strong quality. 38 identified 4 50 sources, interleukins (predictive, indicative, diagnostic) HbA1c (predictive) most prevalent, appearing 48 respectively. Other included predictive blood markers cholesterol, white cell counts), growth factors, bacteria presence (predictive), proteins e.g., matrix metalloproteinases), cellular (indicative Ki-67, alpha-smooth muscle actin [α-SMA]). CONCLUSION: Predictive comorbidities that may hinder healing, aiding pre-amputation risk assessment poor recovery. Indicative monitor key biological processes, such angiogenesis (the formation new vessels), contraction, inflammation. Diagnostic provide direct insights into composition cellular-level healing. Integrating these enables continuous monitoring process while accounting comorbidities, enhancing objectivity post-surgical management ensuring more effective, personalized rehabilitation strategies. Layman's Abstract after amputation can delay negatively impacting health, increasing heart problems death. Currently, health is subjective, no creating a reliable measures. explored (biological like those tissue) indicate, predict, diagnose A was conducted multiple databases, following established Studies if they connected focused on tissue, used ethical, repeatable methods. The studies assessed based research type, type diabetic), (human animal). measure them different identified, two types, (involved inflammation) sugar control marker (predicting healing), being common. tests (cholesterol, counts) levels predict factors indicate progress, changes at level. Biomarkers issues infections nutrition might slow useful assessing non-healing risks before Markers show how progressing tracking decreases inflammation increases growth. detailed information about Using range helps track every stage considers other conditions, leading accurate way manage recovery Article PDF Link: https://jps.library.utoronto.ca/index.php/cpoj/article/view/43717/33685 How To Cite: Williams-Reid H, Johannesson A, Buis A. Wound management, early rehabilitation: Part 3 - biomarkers. Canadian Prosthetics & Orthotics Journal. 2025; Volume 8, Issue 1, No.1. Https://doi.org/10.33137/cpoj.v8i1.43717 Corresponding Author: Professor Arjan Buis, PhDDepartment Biomedical Engineering, Faculty University Strathclyde, Glasgow, Scotland.E-Mail: [email protected] ID: https://orcid.org/0000-0003-3947-293X
Язык: Английский
Процитировано
0