Life Sciences, Год журнала: 2024, Номер unknown, С. 123163 - 123163
Опубликована: Окт. 1, 2024
Язык: Английский
Regenerative Biomaterials, Год журнала: 2024, Номер 11
Опубликована: Янв. 1, 2024
Abstract The imbalance between osteoblasts and osteoclasts is the cause of osteoporosis. Milk-derived extracellular vesicles (mEVs), excellent drug delivery nanocarriers, can promote bone formation inhibit resorption. In this study, we conjugated bone-targeting peptide (AspSerSer, DSS)6 to mEVs by click chemistry then loaded with SRT2104, a SIRT1 (silent mating-type information regulation 2 homolog 1) agonist that was proofed help reduce loss. engineered (DSS)6-mEV-SRT2104 had intrinsic anti-osteoporosis function SRT2104 reverse in homeostasis simultaneously regulating osteogenesis osteoclastogenesis. Furthermore, labelled MnB nanoparticles be used for vivo magnetic resonance imaging (MRI) visualization. obtained nanocomposites significantly prevented loss osteoporosis mice increased mineral density, exhibiting superior accumulation under MRI. We believe proposed (DSS)6-mEV-SRT2104/MnB provides novel paradigm treatment monitoring.
Язык: Английский
Процитировано
5
Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 17, 2025
Milk-derived extracellular vesicles (EVs) show potential applications as nanomedicine; however, their membrane lipids are not fully studied. Herein, we present the lipidomic analysis of extracted EVs and milk fat globules (MFGs) from human breast using ultraperformance supercritical fluid chromatography combined with quadrupole time-of-flight mass spectrometry. A total 11 lipid species, including glycerophospholipids, sphingomyelin (SM), ceramide (Cer), cholesterol (CHOL), 182 molecular species were identified quantified. in ∼4.4% polar lipids, while remaining come MFGs. Lipids rich CHOL, SM, phosphatidylcholine, phosphatidylethanolamine stable during lactational stages. In milk, MFGs have a similar CHOL/SM molar ratio (∼6). Membrane low unsaturation degree high content long-chain saturated fatty acids 24:1 n-9. The unique composition milk-derived compared to can be used study mechanism mRNA delivery infant gastrointestinal tract.
Язык: Английский
Процитировано
0
Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Май 6, 2025
Milk-derived extracellular vesicles (MEV) have received increasing attention due to their physiological benefits for newborn growth and development. However, characteristics immunomodulatory capacities during lactation remain unclear. In this study, we isolated MEV from porcine milk at day 1, week 2, 3 after parturition compared differences by common EV characterization, showing the higher concentration smaller mean size of colostrum. Porcine could be internalized RAW264.7 macrophages functionally deliver immune-related microRNAs (miRNAs). Furthermore, promoted macrophage polarization toward M2-like phenotype exerted anti-inflammatory effects in vitro. Mice models with sepsis also displayed that suppressed secretion pro-inflammatory cytokines serum drove an spleen, ultimately weakening immune responses. Especially, colostrum exhibited most robust on immunoregulation. Overall, study provides valuable information patterns bioactivities.
Язык: Английский
Процитировано
0
Animals, Год журнала: 2025, Номер 15(11), С. 1631 - 1631
Опубликована: Июнь 1, 2025
Extracellular vesicles (EVs) contain bioactive substances and mediate a multitude of physiological functions. EVs can be found in most body fluids are particularly abundant semen. have the potential to become biomarker for quality boar In this study, were isolated from semen relatively young (10 months age, Y-EVs) old (30 O-EVs) duroc boars using ultracentrifugation. The characterized transmission electron microscope, nanoparticle tracking analysis, Western blotting. MicroRNA (miRNA) profiles metabolomes analyzed high-throughput sequencing liquid chromatography–mass spectrometry, respectively. median particle sizes Y-EVs O-EVs 151.3 nm 162.1 nm, miR-148a-3p, miR-10b, miR-21-5p, miR-10a-5p, let-7a, etc., identified as highly enriched miRNAs seminal boars. Comparative analysis revealed 41 differentially expressed 132 differential metabolites between O-EVs. Notably, 18 upregulated O-EVs, such miR-339-5p, miR-125a, miR-423-3p, miR-29c, which mainly endocytosis, focal adhesion, adherens junction. KEGG pathway further indicated that glycerophospholipid metabolism. These results provide an insight into functional roles EVs.
Язык: Английский
Процитировано
0
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12793 - 12815
Опубликована: Ноя. 1, 2024
Plant-derived exosome-like nanovesicles (PELNs) from edible plants, isolated by ultracentrifugation, size exclusion chromatography or other methods, were proved to contain a variety of biologically active and therapeutically specific components. Recently, investigations in the field PELN-based biomedicine have been conducted, which positioned those as promising tools for prevention treatment several diseases, with their natural origin potentially offering superior biocompatibility bioavailability. However, inadequate targeting limited therapeutic effects constrain utility clinical translation PELNs. Thus, strategies aiming at bridging gap engineering PELNs great interest. Those approaches include membrane hybridization, physical chemical surface functionalization encapsulation payloads. Herein, we provide comprehensive overview biogenesis composition, isolation purification methods characterization PELNs, well functions. Current knowledge on construction biomedical application engineered reviewed. Additionally, future directions perspectives this discussed order further enrich expand prospects
Язык: Английский
Процитировано
2
Food Bioscience, Год журнала: 2024, Номер unknown, С. 105119 - 105119
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
1
Life Sciences, Год журнала: 2024, Номер unknown, С. 123163 - 123163
Опубликована: Окт. 1, 2024
Язык: Английский
Процитировано
0