Extracellular Vesicles in Therapeutics: A Comprehensive Review on Applications, Challenges, and Clinical Progress DOI Creative Commons

Jiyoung Goo,

Yeji Lee, Jeongmin Lee

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(3), С. 311 - 311

Опубликована: Фев. 22, 2024

Small Extracellular Vesicles (sEVs) are typically 30-150 nm in diameter, produced inside cells, and released into the extracellular space. These vesicles carry RNA, DNA, proteins, lipids that reflect characteristics of their parent enabling communication between cells alteration functions or differentiation target cells. Owing to these properties, sEVs have recently gained attention as potential carriers for functional molecules drug delivery tools. However, use a therapeutic platform faces limitations, such challenges mass production, purity issues, absence established protocols characterization methods. To overcome these, researchers exploring engineering various applications. This review discusses origins effects, proposing areas needing intensive study. It covers cell-derived natural state engineered forms specific purposes. Additionally, details sources subsequent purification also outlines requirements successful clinical trials, including methods large-scale production purification. Finally, we discuss progress ongoing trials implications future healthcare, offering comprehensive overview latest research sEV

Язык: Английский

Exosome engineering in cell therapy and drug delivery DOI Open Access
Somayeh Sadeghi, Fahimeh Ramezani Tehrani, Safa Tahmasebi

и другие.

Inflammopharmacology, Год журнала: 2023, Номер 31(1), С. 145 - 169

Опубликована: Янв. 7, 2023

Язык: Английский

Процитировано

177

Strategies for Targeted Delivery of Exosomes to the Brain: Advantages and Challenges DOI Creative Commons
Hojun Choi, Kyungsun Choi, Dae‐Hwan Kim

и другие.

Pharmaceutics, Год журнала: 2022, Номер 14(3), С. 672 - 672

Опубликована: Март 18, 2022

Delivering therapeutics to the central nervous system (CNS) is difficult because of blood-brain barrier (BBB). Therapeutic delivery across tight junctions BBB can be achieved through various endogenous transportation mechanisms. Receptor-mediated transcytosis (RMT) one most widely investigated and used methods. Drugs hijack RMT by expressing specific ligands that bind receptors mediating transcytosis, such as transferrin receptor (TfR), low-density lipoprotein (LDLR), insulin (INSR). Cell-penetrating peptides viral components originating from neurotropic viruses also utilized for efficient crossing therapeutics. Exosomes, or small extracellular vesicles, have gained attention natural nanoparticles treating CNS diseases, owing their potential broad surface engineering capability. RMT-mediated transport exosomes LDLR-targeting apolipoprotein B has shown promising results. Although surface-modified possessing brain targetability enhanced in preclinical studies, successful development clinically approved exosome diseases requires establishment quantitative qualitative methods monitoring exosomal parenchyma vivo well elucidation mechanisms underlying exosomes.

Язык: Английский

Процитировано

78

Revolutionizing anti-tumor therapy: unleashing the potential of B cell-derived exosomes DOI Creative Commons

Jingwen Xiong,

Hao Chi,

Guanhu Yang

и другие.

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Июнь 5, 2023

B cells occupy a vital role in the functioning of immune system, working tandem with T to either suppress or promote tumor growth within microenvironment(TME). In addition direct cell-to-cell communication, and other release exosomes, small membrane vesicles ranging size from 30-150 nm, that facilitate intercellular signaling. Exosome research is an important development cancer research, as they have been shown carry various molecules such major histocompatibility complex(MHC) integrins, which regulate TME. Given close association between TME development, targeting substances has emerged promising strategy for therapy. This review aims present comprehensive overview contributions made by exosomes microenvironment (TME). Additionally, we delve into potential cell-derived progression cancer.

Язык: Английский

Процитировано

58

Towards artificial intelligence-enabled extracellular vesicle precision drug delivery DOI Creative Commons
Zachary Greenberg, Kiley Graim, Mei He

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 199, С. 114974 - 114974

Опубликована: Июнь 24, 2023

Язык: Английский

Процитировано

52

Exosome-based delivery strategies for tumor therapy: an update on modification, loading, and clinical application DOI Creative Commons
Qian Yang, Shisheng Li,

Haibo Ou

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 28, 2024

Malignancy is a major public health problem and among the leading lethal diseases worldwide. Although current tumor treatment methods have therapeutic effect to certain extent, they still some shortcomings such as poor water solubility, short half-life, local systemic toxicity. Therefore, how deliver agent so realize safe effective anti-tumor therapy become urgently be solved in this field. As medium of information exchange material transport between cells, exosomes are considered promising drug delivery carrier due their nano-size, good biocompatibility, natural targeting, easy modification. In review, we summarize recent advances isolation, identification, loading, modification carriers for alongside application therapy. Basic knowledge exosomes, biogenesis, sources, characterization methods, also introduced herein. addition, challenges related use vehicles discussed, along with future trends. This review provides scientific basis exosome systems oncological

Язык: Английский

Процитировано

51

Recent advances in the delivery and applications of nonviral CRISPR/Cas9 gene editing DOI Open Access
Frazer Sinclair, Anjuman Ara Begum, Charles C. Dai

и другие.

Drug Delivery and Translational Research, Год журнала: 2023, Номер 13(5), С. 1500 - 1519

Опубликована: Март 29, 2023

Язык: Английский

Процитировано

47

Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review DOI Creative Commons
Zhen Chen, Min Xiong, Jiaqi Tian

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Янв. 3, 2024

Abstract Exosomes are nanoscale extracellular vesicles secreted by cells and enclosed a lipid bilayer membrane containing various biologically active cargoes such as proteins, lipids, nucleic acids. Engineered exosomes generated through genetic modification of parent show promise drug delivery vehicles, they have been demonstrated to great therapeutic potential for treating cancer, cardiovascular, neurological, immune diseases, but systematic knowledge is lacking regarding optimization loading assessment efficacy. This review summarizes current approaches engineering evaluating their effects, techniques assessing exosome release kinetics, cell targeting, biodistribution, pharmacokinetics, outcomes critically examined. Additionally, this synthesizes the latest applications in clinical translation. The compiled provides framework rational design rigorous therapeutics. Continued advancement robust characterization methods reporting standards will accelerate development technologies pave way studies. Graphical

Язык: Английский

Процитировано

46

Exosome-mediated delivery and regulation in neurological disease progression DOI Creative Commons
Gurpreet Singh,

Ankit Mehra,

Sanchit Arora

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 264, С. 130728 - 130728

Опубликована: Март 11, 2024

Exosomes (EXOs), minute membranous structures originating from diverse biological sources, have recently seized the attention of researchers due to their theranostic potential for neurological diseases. Released actively by various cells, including stem adipose tissue, and immune EXOs wield substantial regulatory influence over intricate landscape complications, exhibiting both positive negative modulatory effects. In AD, play a pivotal role in disseminating breaking down amyloid-β protein. Moreover, derived mesenchymal cells showcase remarkable capacity mitigate pro-inflammatory phenotypes regulating miRNAs neurodegenerative These vesicles possess unique ability traverse blood-brain barrier, governing aggregation mutant huntingtin Understanding exosomal functions within CNS holds significant promise enhancing treatment efficacy This review intricately examines mechanisms involving disease development, highlighting therapeutic prospects exploring utility exosome-based nanomedicine complications. Additionally, delves into challenges associated with drug delivery brain, emphasizing complexities inherent this critical aspect neurotherapeutics.

Язык: Английский

Процитировано

38

Harnessing extracellular vesicle heterogeneity for diagnostic and therapeutic applications DOI
Randy P. Carney, Rachel R. Mizenko, Batuhan Turhan Bozkurt

и другие.

Nature Nanotechnology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 28, 2024

Язык: Английский

Процитировано

28

Insight into the Functional Dynamics and Challenges of Exosomes in Pharmaceutical Innovation and Precision Medicine DOI Creative Commons
Anu Sharma, Anita Yadav,

Aparajita Nandy

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(6), С. 709 - 709

Опубликована: Май 24, 2024

Of all the numerous nanosized extracellular vesicles released by a cell, endosomal-originated exosomes are increasingly recognized as potential therapeutics, owing to their inherent stability, low immunogenicity, and targeted delivery capabilities. This review critically evaluates transformative of exosome-based modalities across pharmaceutical precision medicine landscapes. Because precise biomolecular cargo delivery, posited ideal candidates in drug enhancing regenerative strategies, advancing diagnostic technologies. Despite significant market growth projections exosome therapy, its utilization is encumbered substantial scientific regulatory challenges. These include lack universally accepted protocols for isolation complexities associated with navigating environment, particularly guidelines set forth U.S. Food Drug Administration (FDA). presents comprehensive overview current research trajectories aimed at addressing these impediments discusses prospective advancements that could substantiate clinical translation exosomal therapies. By providing analysis both capabilities hurdles therapeutic applications, this article aims inform direct future paradigms, thereby fostering integration systems into mainstream practice.

Язык: Английский

Процитировано

21