Analytical Methods,
Год журнала:
2024,
Номер
17(3), С. 533 - 538
Опубликована: Дек. 10, 2024
The
superoxide
anion
(O2˙-)
is
a
vital
reactive
oxygen
species
(ROS)
and
participates
in
various
physiological
pathological
processes
organisms.
outbreak
of
O2˙-
the
endoplasmic
reticulum
(ER)
believed
to
be
closely
related
many
inflammatory
diseases.
In
this
work,
turn-on
type
ER-targeting
fluorescent
probe
ERO
was
rationally
designed
for
sensitive
selective
detection
O2˙-.
minimum
limit
concentration
about
3.3
×
10-7
M
aqueous
solution.
More
importantly,
has
minimal
biological
toxicity
can
effectively
target
ER
detect
macrophage
cells.
Resveratrol
natural
drug
anti-inflammatory
effects.
Through
fluorescence
monitoring
ERO,
it
found
that
there
an
acute
spondylitis
mice
resveratrol
reversed
increase.
Thus,
potential
become
favorable
diagnostic
tool
visualize
mouse
spine
during
inflammation
therapeutic
effect
resveratrol.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(3), С. 997 - 1002
Опубликована: Янв. 4, 2024
Single-atom
catalysts
(SACs),
specifically
iron
single
atoms
dispersed
on
nitrogen-doped
carbon
(Fe-NC),
have
shown
promising
potential
in
the
electrocatalytic
reduction
of
nitrate
to
ammonia
(NitRR),
but
there
is
a
lack
understanding
their
intrinsic
activity.
The
conventional
measurements
often
overlook
performance
SACs,
leading
significant
underestimation.
This
study
presents
an
situ
electrochemical
probing
protocol,
using
two
poisoning
molecules
(SCN–
and
NO2–),
characterize
reactivity
Fe
sites
Fe-NC
SACs
for
NitRR.
technique
aids
quantifying
yield
rate
active
site
number.
findings
reveal
turnover
frequency
(TOF)
based
number
sites,
challenging
current
SACs'
inherent
performances.
unique
approach
holds
considerable
determining
activity
other
complex
reactions,
opening
new
avenues
exploration
processes.
Membranes,
Год журнала:
2025,
Номер
15(1), С. 9 - 9
Опубликована: Янв. 1, 2025
The
endoplasmic
reticulum
and
the
internal
nuclear
compartments
are
intrinsically
connected
through
membrane,
pores
lamina.
High
resolution
imaging
of
each
these
cellular
features
concurrently
remains
a
significant
challenge.
To
that
end
we
have
developed
new
molecular
membrane-endoplasmic
(NM-ER)
staining
fluorophore
with
emission
maxima
at
650
nm.
NM-ER
is
compatible
fixed
live
cell
stimulated
depletion
microscopy
(STED)
showing
improvement
in
when
compared
to
comparable
confocal
laser
scanning
microscopy.
versatility
was
illustrated
its
use
other
fluorophores
for
co-imaging
DNA,
lamina
allowing
abnormalities
be
identified.
alone,
or
region
labels
could
an
important
tool
investigation
transport
associated
processes.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
The
measurement
of
microviscosity
gradients
across
different
regions
self-assembled
systems
is
crucial
for
optimizing
their
performance
in
both
biological
and
industrial
applications.
However,
this
task
has
long
been
challenging
due
to
the
spatial
barrier
within
systems,
interference
from
polarity
gradients,
lack
region-positioned
probes.
To
overcome
these
challenges,
we
developed
three
kinds
length-regulated
aggregation-induced
emission
(AIE)
probes
resolution
microviscosity.
These
AIE
with
varying
alkyl
chain
lengths
showed
high
sensitivity
(0.81)
over
a
viscosity
range
2
435
mPa
s
unaffected
luminescence
environmental
polarity,
ensuring
accurate
measurements
diverse
environments.
Using
micelles
as
model
were
able
localize
selectively
ranging
hydrophobic
core
hydrophilic
shell
interface.
results
indicated
that
was
highest
gradually
decreased
toward
outer
regions.
Furthermore,
successfully
applied
monitoring
hydrogels
food
thickeners,
showing
strong
correlation
among
fluorescence
intensity,
tensile
strength,
thickening
effects.
findings
underscore
potential
evaluating
Materials Today Bio,
Год журнала:
2025,
Номер
32, С. 101654 - 101654
Опубликована: Март 11, 2025
Lung
carcinoma
is
the
leading
cause
of
mortality
globally,
posing
a
significant
public
health
concern.
Fluorescent-mediated
tumor
imaging
emerging
as
novel
diagnostic
and
therapeutic
approach
in
clinical
practice.
Nevertheless,
traditional
probes
lack
accuracy
diagnosing
tumors
due
to
overlap
baseline
values
certain
biomarkers
between
normal
cells
both
exhibit
turn-on
fluorescence,
rendering
it
impossible
distinguish
tissue
from
with
high
resolution.
We
introduce
sensing
strategy
that
constructs
probe
an
elevated
biomarker
response-threshold
targeting
ability
for
endoplasmic
reticulum
(ER),
enabling
precise
distinction
cells,
successfully
develop
such
probe.
Elevating
advantageous
minimizing
interference
cells.
Additionally,
ER
ensures
probe's
response
range
consistent
content
ER,
collectively
enhancing
differentiation
cancer
Using
this
probe,
distinct
bright
fluorescence
signal
could
be
observed
confocal
tissues
tumor-bearing
mice
after
intravenous
injection,
stark
contrast
limited
emanating
tissues.
Furthermore,
demonstrated
exceptional
precision
distinguishing
lung
para-cancer
tissue.
This
work
presents
more
reliable
detection
strategy,
capable
accurate
diagnosis
even
when
highly
expressed
It
promises
valuable
tool
future
applications,
particularly
intraoperative
assisted
resection.
Abstract
The
reactive
oxygen
species
(ROS)
amplification
caused
by
inevitable
plasma
albumin
encapsulation
is
still
a
challenge
to
circumvent
the
systemic
adverse
effects
in
photodynamic
therapy
(PDT)
process.
Herein,
disulfide
bond
linked
homodimer,
Cy1280,
which
modulated
accurately
balance
fluorescence
and
ROS
generation
exhibit
weak
sealed
PDT
effect
during
blood
circulation,
exploited.
Cy1280
can
be
specifically
internalized
dispersed
at
tumor
site
via
Organic
Anion
Transporter
Proteins
(OATPs)
thiol‐disulfide
exchange
mediated
synergistic
uptake
activated
after
mild
sunlight
irradiation
(100
±
5
Klx)
sensitize
neighboring
cellular
mitochondria
execute
direct
protein
dysfunction
effect.
dynamic
covalent
chemistry
(DCC)
facilitates
prolonged
sustained
retention
tumors
(>336
h)
demonstrates
efficacy
of
imaging‐guided
solid‐tumor
tumor‐bearing
BALB/C
mice.
This
study
resolves
stubborn
impotent
penetration
bulky‐sized
nanoparticles
high
interstitial
pressure
with
manner,
long‐term
circulation
manipulated
also
improve
whole
body
phototoxic
symptom.
advantageous
feature
provides
promising
candidate
for
overcoming
off‐target
phototoxicity
inadequate
accumulation
challenges
clinical
translation
photosensitizers
(PSs).
ACS Agricultural Science & Technology,
Год журнала:
2024,
Номер
4(3), С. 337 - 344
Опубликована: Фев. 28, 2024
The
two
main
types
of
cholinesterases,
acetylcholinesterase
(AChE)
and
butyrylcholinesterase
(BChE),
share
similar
structures
functions
are
commonly
present
in
biological
environments.
activity
cholinesterase
is
closely
related
to
pesticide
residues;
it
crucial
develop
enzyme-inhibition-based
fluorescent
probes
for
residue
detection.
However,
the
discovery
a
probe
that
selectively
targets
one
these
consistently
formidable
challenge.
In
this
study,
kinds
probes,
HBT-A
HBT-B,
were
developed
with
2-(2-hydroxyphenyl)
benzothiazole
(HBT)
as
fluorophore,
which
can
distinguish
AChE
BChE,
respectively.
Our
(HBT-A
HBT-B
BChE)
exhibited
high
sensitivity
specificity
toward
their
respective
analytes
cells
zebrafish.
Finally,
we
used
an
example
demonstrated
be
effective
tool
visualizing
residues
living
organisms,
highlighting
potential
application
environmental
monitoring
food
safety.
Chemical Communications,
Год журнала:
2024,
Номер
60(43), С. 5618 - 5621
Опубликована: Янв. 1, 2024
A
tyrosinase-activatable
fluorescent
probe
with
endoplasmic
reticulum
targetability
was
developed
for
the
first
time.
It
can
ratiometrically
fluoresce
and
hence
be
used
to
monitor
refluxed
tyrosinase
into
reticulum.
