Frontiers in Endocrinology,
Год журнала:
2022,
Номер
13
Опубликована: Ноя. 9, 2022
Purpose
Human
follicular
fluid
(FF)
is
a
complex
biological
that
contributes
to
the
micro-environment
of
oocyte
development.
The
aim
this
study
was
evaluate
role
steroid
and
gonadotropic
hormones
levels
mitochondrial
function
in
embryo
development
during
vitro
fertilization
cycles.
Methods
This
cohort
138
women
receiving
IVF/ICSI,
including
136
FF
samples
from
109
infertile
women.
were
tested
by
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
immunoassays.
mRNA
expression
electron
transport
chain
(ETC)
genes
exosomes
detected
qPCR.
Results
Analysis
these
individual
concentrations
revealed
LH
FSH
higher
follicles
which
developed
into
top
quality
(TQ)
blastocyst
(LH:
9.44
±
2.32mIU/ml,
FSH:
9.32
1.01mIU/ml)
than
those
there
failure
5.30
0.84mIU/ml,
6.91
0.62mIU/ml).
In
contrast,
cortisone
lower
for
oocytes
resulted
TQ
(12.20
0.82mIU/ml).
receiver
operating
characteristic
analysis
showed
predicted
with
excellent
AUC
value
0.711
0.747.
Mitochondrial
ETC
I
III
increased
blastocyst.
Correlation
positively
correlated
FF.
Conclusion
single
follicle
can
predetermine
subsequent
some
extent.
Furthermore,
impaired
exosome
dysfunction
potiential
event
causes
hormone
change
Frontiers in Cell and Developmental Biology,
Год журнала:
2021,
Номер
9
Опубликована: Март 19, 2021
Meiosis
is
the
basis
of
sexual
reproduction.
In
female
mammals,
meiosis
oocytes
starts
before
birth
and
sustains
at
dictyate
stage
meiotic
prophase
I
gonadotropins-induced
ovulation
happens.
Once
gets
started,
undergo
leptotene,
zygotene,
pachytene
stages,
then
arrest
stage.
During
each
estrus
cycle
in
or
menstrual
humans,
a
small
portion
within
preovulatory
follicles
may
resume
meiosis.
It
crucial
for
females
to
supply
high
quality
mature
sustaining
fertility,
which
generally
achieved
by
fine-tuning
oocyte
resumption
progression.
Anything
that
disturbs
process
result
failure
oogenesis
seriously
affect
both
fertility
health
females.
Therefore,
uncovering
regulatory
network
progression
illuminates
not
only
how
foundations
mammalian
reproduction
are
laid,
but
mis-regulation
these
steps
infertility.
order
provide
an
overview
recently
uncovered
cellular
molecular
mechanism
during
maturation,
especially
epigenetic
modification,
progress
including
induced
gonadotropins
summarized.
Then,
advances
aspects,
such
as
histone
acetylation,
phosphorylation,
methylation,
glycosylation,
ubiquitination,
SUMOylation
related
maturation
reviewed.
Frontiers in Endocrinology,
Год журнала:
2023,
Номер
14
Опубликована: Дек. 13, 2023
The
ovarian
microenvironment
is
critical
for
follicular
development
and
oocyte
maturation.
Maternal
conditions,
including
polycystic
ovary
syndrome
(PCOS),
endometriosis,
aging,
may
compromise
the
microenvironment,
development,
quality.
Chronic
low-grade
inflammation
can
induce
oxidative
stress
tissue
fibrosis
in
ovary.
In
PCOS,
pro-inflammatory
cytokine
levels
are
often
elevated
fluids.
women
with
obesity
hyperandrogenemia
insulin
resistance
chronic
inflammation,
thereby
disrupting
by
increasing
stress.
endometrioma-derived
iron
overload
stress,
leading
to
ferroptosis
fibrosis.
inflammatory
aging
(inflammaging),
senescent
cells
secrete
senescence-associated
secretory
phenotype
factors,
causing
Therefore,
controlling
would
present
a
novel
therapeutic
strategy
improving
minimizing
dysfunction.
Annual Review of Cell and Developmental Biology,
Год журнала:
2021,
Номер
37(1), С. 199 - 232
Опубликована: Июль 6, 2021
Nuclei
are
central
hubs
for
information
processing
in
eukaryotic
cells.
The
need
to
fit
large
genomes
into
small
nuclei
imposes
severe
restrictions
on
genome
organization
and
the
mechanisms
that
drive
genome-wide
regulatory
processes.
How
a
disordered
polymer
such
as
chromatin,
which
has
vast
heterogeneity
its
DNA
histone
modification
profiles,
folds
discernibly
consistent
patterns
is
fundamental
question
biology.
Outstanding
questions
include
how
spatially
temporally
organized
regulate
cellular
processes
with
high
precision
whether
causally
linked
transcription
regulation.
advent
of
next-generation
sequencing,
super-resolution
imaging,
multiplexed
fluorescent
situ
hybridization,
single-molecule
imaging
individual
living
cells
caused
resurgence
efforts
understand
spatiotemporal
genome.
In
this
review,
we
discuss
structural
mechanistic
properties
at
different
length
scales
examine
changes
higher-order
chromatin
during
important
developmental
transitions.
Journal of Assisted Reproduction and Genetics,
Год журнала:
2022,
Номер
39(4), С. 905 - 918
Опубликована: Март 21, 2022
Abstract
In
mammalian
species
an
optimal
fertilization
window
during
which
successful
occurs.
the
majority
of
mammals
estrus
marks
ovulation
time
and
coincident
with
mating,
thereby
allowing
synchronized
meeting
in
fallopian
tubes,
between
freshly
ejaculated
sperm
ovulated
oocytes.
Conversely,
women
do
not
show
natural
visual
signs
such
that
can
occur
hours
later
involving
aged
oocyte
spermatozoa.
During
this
time,
undergoes
a
rapid
degradation
known
as
“postovulatory
aging”
(POA).
POA
may
become
particularly
important
human-assisted
reproductive
technologies,
retrieved
mature
oocytes
be
delayed
due
to
increased
laboratory
workload
or
because
unforeseeable
circumstances,
like
availability
semen
samples.
This
paper
is
updated
review
consequences
POA,
either
vivo
vitro
,
on
quality
particular
attention
modifications
caused
by
nuclear,
cytoplasmic,
genomic,
epigenetic
maturation,
embryo
development.
Reproductive Biology and Endocrinology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Окт. 2, 2023
Abstract
In
human
female
primordial
germ
cells,
the
transition
from
mitosis
to
meiosis
begins
fetal
stage.
is
arrested
at
diplotene
stage
of
prophase
in
I
(MI)
after
synapsis
and
recombination
homologous
chromosomes,
which
cannot
be
segregated.
Within
follicle,
maintenance
oocyte
meiotic
arrest
primarily
attributed
high
cytoplasmic
concentrations
cyclic
adenosine
monophosphate
(cAMP).
Depending
on
specific
species,
oocytes
can
remain
for
extended
periods
time,
ranging
months
even
years.
During
estrus
phase
animals
or
menstrual
cycle
humans,
resumption
occurs
certain
due
a
surge
luteinizing
hormone
(LH)
levels.
Any
factor
interfering
with
this
process
may
lead
impaired
maturation,
turn
affects
reproductive
function.
Nevertheless,
precise
molecular
mechanisms
underlying
phenomenon
has
not
been
systematically
summarized
yet.
To
provide
comprehensive
understanding
recently
uncovered
regulatory
network
involved
development
progress
cellular
nuclear
maturation
including
summarized.
Additionally,
advancements
events
occurring
oocytes,
such
as
maternal
mRNA
degradation,
posttranslational
regulation,
organelle
distribution
associated
quality
are
reviewed.
Therefore,
pathways
regulating
will
detailed
insight
into
system
theoretical
basis
further
research
potential
approaches
novel
disease
treatments.
Reproductive Biology and Endocrinology,
Год журнала:
2025,
Номер
23(S1)
Опубликована: Март 10, 2025
Abstract
It
is
widely
recognized
that
luteinizing
hormone
(LH)
activity
pivotal
during
folliculogenesis.
Nonetheless,
the
use
of
LH
ovarian
stimulation
remains
a
matter
debate.
Indeed,
women
with
good
function
are
able
to
sustain
follicle
growth
and
maturation
carried
out
regimens
based
on
follicle-stimulating
(FSH)
alone.
However,
evidence
exists
could
be
necessary
in
specific
infertile
subgroups
undergoing
assisted
reproduction
treatment
(ART)
who
characterized
by
functional
or
constitutive
deficiency.
For
instance,
reduced
sensitivity
gonadotropins,
also
called
hypo-responders,
usually
present
genetic
condition
impair
LH.
Furthermore,
advanced
reproductive
age
less
system
consequently
androgen
production.
Reduced
represent
main
criteria
proposed
POSEIDON
group
identify
impaired
prognosis
when
ART.
Hypogonadotropic
hypogonadal
undetectable
levels,
thus
addition
mandatory
achieve
satisfactory
follicular
recruitment.
The
aim
review
describe
role
recombinant
stimulation,
identifying
population
for
whom
supplementation
improve
outcome
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(18), С. 10110 - 10110
Опубликована: Сен. 18, 2021
Follicle
stimulating
hormone
(FSH)
is
produced
by
the
pituitary
gland
in
a
coordinated
hypothalamic–pituitary–gonadal
(HPG)
axis
event,
plays
important
roles
reproduction
and
germ
cell
development
during
different
phases
of
reproductive
(fetal,
neonatal,
puberty,
adult
life),
consequently
essential
for
fertility.
FSH
heterodimeric
glycoprotein
two
dissociable
subunits,
α
β.
The
β-subunit
(FSHβ)
function
starts
upon
coupling
to
its
specific
receptor:
follicle-stimulating
receptor
(FSHR).
FSHRs
are
localized
mainly
on
surface
target
cells
testis
ovary
(granulosa
Sertoli
cells)
have
recently
been
found
testicular
stem
extra-gonadal
tissue.
Several
disorders
associated
with
absent
or
low
secretion,
mutation
receptor,
and/or
signaling
pathways.
However,
influence
still
poorly
understood;
some
studies
suggested
that
this
also
determinant
role
self-renewal
germinative
acts
increase
undifferentiated
spermatogonia
proliferation.
In
addition,
vitro,
together
other
factors,
it
assists
process
differentiation
primordial
(PGCLCs)
into
gametes
(oocyte-like
SSCLCs).
review,
we
describe
relevant
research
spermatogenesis
folliculogenesis,
humans
species.
possible
generation
vitro
presented.
